LOCUS       sequence-202478-       12519 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of FLAG Myc tagged A.thaliana-Hen1 (codon optimised for 
            Drosophila) in Drosophila using somatic tissue Gal4 drivers.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12519)
  AUTHORS   Alberti C, Manzenreither RA, Sowemimo I, Burkard TR, Wang J, 
            Mahofsky K, Ameres SL, Cochella L
  TITLE     Cell-type specific sequencing of microRNAs from complex animal 
            tissues.
  JOURNAL   Nat Methods. 2018 Feb 26. pii: nmeth.4610. doi: 10.1038/nmeth.4610.
  PUBMED    29481550
REFERENCE   2  (bases 1 to 12519)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2018 Feb 26. pii: nmeth.4610. doi: 10.1038/nmeth.4610."
COMMENT     Sequence Label: pUASt-FMW-attB-AthHen1
FEATURES             Location/Qualifiers
     source          1..12519
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     132..150
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(188..210)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     310..329
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     misc_feature    join(867..897,898..1099)
                     /label=P element 3' end
                     /note="P element 3' end"
     protein_bind    1161..1255
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        1274..1512
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     1459..1480
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     regulatory      1626..1635
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1635..1700
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     regulatory      1716..1725
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1725..1754
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1764..1793
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1803..1832
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1842..1871
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1881..1910
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1944..1973
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    1986..2010
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4876..4900)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     intron          4991..5056
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             5186..5206
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    5478..5612
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5515..5534)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5569..5588
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5639..5657)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(6109..6180,6582..6855,6930..7584,7646..7961,8165..8296,
                     8367..8981)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     misc_feature    join(9760..10314,10315..10345)
                     /label=P element 5' end
     rep_origin      complement(10580..11168)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10660..10679)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11339..12130,12131..12199))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11962..11981
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12200..12304)
                     /gene="bla"
                     /label=AmpR promoter
     protein_bind    12351..12420
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
ORIGIN
        1 gtcacggtga gcacgggacg tgcgacggcg tcggcgggtg cggatacgcg gggcagcgtc
       61 agcgggttct cgacggtcac ggcgggcatg tcgactaaga aaccattatt atcatgacat
      121 taacctataa aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg
      181 gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg
      241 ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc
      301 ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac
      361 cgcaccgaat cgcgcggaac taacgacagt cgctccaagg tcgtcgaaca aaaggtgaat
      421 gtgttgcgga gagcgggtgg gagacagcga aagagcaact acgaaacgtg gtgtggtgga
      481 ggtgaattat gaagagggcg cgcgatttga aaagtatgta tataaaaaat atatcccggt
      541 gttttatgta gcgataaacg agtttttgat gtaaggtatg caggtgtgta agtcttttgg
      601 ttagaagaca aatccaaagt ctacttgtgg ggatgttcga aggggaaata cttgtattct
      661 ataggtcata tcttgttttt attggcacaa atataattac attagctttt tgagggggca
      721 ataaacagta aacacgatgg taataatggt aaaaaaaaaa aacaagcagt tatttcggat
      781 atatgtcggc tactccttgc gtcgggcccg aagtcttaga gccagatatg cgagcacccg
      841 gaagctcacg atgagaatgg ccagaccatg atgaaataac ataaggtggt cccgtcggca
      901 agagacatcc acttaacgta tgcttgcaat aagtgcgagt gaaaggaata gtattctgag
      961 tgtcgtattg agtctgagtg agacagcgat atgattgttg attaaccctt agcatgtccg
     1021 tggggtttga attaactcat aatattaatt agacgaaatt atttttaaag ttttattttt
     1081 aataatttgc gagtacgcaa agcttggctg catccaacgc gttgggagct ctccggatcc
     1141 aagcttgcat gcctgcaggt cggagtactg tcctccgagc ggagtactgt cctccgagcg
     1201 gagtactgtc ctccgagcgg agtactgtcc tccgagcgga gtactgtcct ccgagcggag
     1261 actctagcga gcgccggagt ataaatagag gcgcttcgtc tacggagcga caattcaatt
     1321 caaacaagca aagtgaacac gtcgctaagc gaaagctaag caaataaaca agcgcagctg
     1381 aacaagctaa acaatctgca gtaaagtgca agttaaagtg aatcaattaa aagtaaccag
     1441 caaccaagta aatcaactgc aactactgaa atctgccaag aagtaattat tgaatacaag
     1501 aagagaactc tgaataggga attgggaatt atcgaggcct gtctagagaa gcttgttcga
     1561 atctcgagtg cgcgcttccg gaggtataca cctaggcggt accactgcag tgaattcgga
     1621 gctccgccac catggactac aaagaccatg acggtgatta taaagatcat gacatcgatt
     1681 acaaggatga cgatgacaag caccggttga gctccgccac catggagcaa aagctcattt
     1741 ctgaagagga cttgaatgaa atggagcaaa agctcatttc tgaagaggac ttgaatgaaa
     1801 tggagcaaaa gctcatttct gaagaggact tgaatgaaat ggagcaaaag ctcatttctg
     1861 aagaggactt gaatgaaatg gagcaaaagc tcatttctga agaggacttg aatgaaatgg
     1921 agagcttggg cgacctcacc atggagcaaa agctcatttc tgaagaggac ttgaatcacc
     1981 ggtatacaag tttgtacaaa aaagcaggct ccgcggccgc ccccttcacc atggcgggtg
     2041 gtggcaagca caccccgact ccaaaggcta ttatccatca aaagtttggc gctaaggcct
     2101 cgtatacagt ggaggaagtt catgactcgt cgcagtcggg ctgcctgggc ctggccattc
     2161 cgcagaaggg tccgtgcctc taccggtgtc acctgcagct gccagaattc tccgtggtga
     2221 gcaatgtatt caaaaagaag aaggatagtg agcagagtgc tgctgagctg gccttggata
     2281 aactgggtat tcgaccccag aacgacgatt tgacagtgga tgaggcgcgc gatgagatcg
     2341 tgggcaggat caaatatata ttcagtgacg agttcctgag tgccgagcac ccgttgggcg
     2401 cacacttgcg cgcagccctg cgtcgcgatg gcgaacgttg cggctcggtt ccggttagtg
     2461 tgattgctac cgtggacgcc aaaattaaca gccgctgtaa gataatcaat ccctccgtgg
     2521 agagtgaccc gttcctggcc atttcgtacg tgatgaaagc agcagctaag ctggccgact
     2581 acatagtggc ctcgccacac ggcctccgtc gcaaaaacgc gtacccgtcc gagatagtcg
     2641 aggcgttggc gacgcacgta agcgattcgc tccattcccg ggaggtggcc gctgtttaca
     2701 ttccgtgcat cgatgaggag gttgtagagt tggatacgct ctacatcagc tcgaatcgcc
     2761 attacctgga ttcgatcgcc gagcgactcg gtttgaagga cggcaatcag gttatgatta
     2821 gtcgcatgtt tggcaaggcg tcgtgtggtt ccgagtgtcg tctgtactcg gagatcccaa
     2881 agaagtatct ggacaattcc agcgatgcca gcggtacttc caacgaggat tccagccata
     2941 tcgtcaagag ccgcaacgct cgcgcctcgt acatctgtgg tcaggatatc catggtgacg
     3001 ccatcctcgc cagcgtagga taccgatgga agtcggacga cctggactac gatgatgtga
     3061 cagtcaacag tttctacagg atatgctgcg gcatgtcccc gaatggtata tacaaaataa
     3121 gcaggcaggc cgtgatcgcg gcgcagctgc ccttcgcctt tactacgaag agcaattggc
     3181 gcggtccgtt gccccgtgag atcctgggtc tgttctgcca ccagcaccga ctggcggaac
     3241 caattctgtc cagcagtacc gcgccggtga agagtttgtc cgacatcttt cgcagtcaca
     3301 agaagttgaa ggtaagcggt gttgacgacg caaacgagaa cttgtcccgc cagaaggaag
     3361 atacgcccgg actgggtcac ggcttccgat gtgaggttaa gatttttacc aagagtcagg
     3421 acctggtact cgaatgctcc ccacgtaaat tctacgagaa ggagaacgat gccatccaga
     3481 acgcctcctt gaaggccttg ctctggttca gcaagttctt tgccgacctg gacgttgacg
     3541 gcgagcaaag ttgcgacact gatgatgatc aggatacgaa gagcagtagc ccaaacgtat
     3601 tcgccgcccc ccccatcttg cagaaggagc actcctcgga gagcaagaac accaatgtgc
     3661 tcagtgcgga aaagcgggtc cagtccatca ccaatggcag tgtcgtgagt atttgctact
     3721 ccctcagcct ggccgtcgat cccgagtatt cgtccgatgg cgaaagcccc agggaggata
     3781 atgaaagtaa cgaggagatg gaaagtgaat acagcgccaa ctgtgagtcg agtgtggagc
     3841 tgattgagtc gaacgaggag atcgagttcg aggtcggcac cggcagcatg aacccgcaca
     3901 ttgagagcga ggtgacccaa atgaccgttg gtgagtacgc cagctttcgc atgacaccgc
     3961 cagacgcggc agaggcactg atcctggcag tgggcagcga caccgtgcga atccgctccc
     4021 tgctgagcga gaggccatgc ctcaattaca acatcctgct gctgggcgtt aagggaccca
     4081 gtgaggagcg catggaggcc gccttcttca agccccctct ctcgaagcaa cgagtggagt
     4141 atgccctgaa gcatattcga gagtcgagtg catcgacgct ggtggatttt ggctgtggct
     4201 ccggaagtct gttggatagt ctcctggact acccaacaag cctgcaaacg attataggcg
     4261 tggacatctc gcctaagggc ctcgcccgtg ccgcgaagat gctgcacgtt aaattgaaca
     4321 aggaggcgtg caatgttaag agtgctactc tgtacgatgg aagcattctc gagttcgaca
     4381 gccgcctgca cgatgttgac attggcactt gtttggaagt gattgaacac atggaagagg
     4441 atcaagcctg cgagtttggt gaaaaggtgt tgagtttgtt ccacccgaag ctgctcatag
     4501 tgagcacccc caattatgaa tttaacacga tactgcagcg ctcgacgccc gagacgcagg
     4561 aggagaacaa ctccgagcca cagctcccga aattcaggaa ccatgaccat aaattcgagt
     4621 ggacacggga gcagtttaat caatgggctt cgaagctggg caagcgccac aactactccg
     4681 tggagttcag cggtgtcgga ggcagtggcg aggtggaacc gggctttgcc agccagatcg
     4741 ctatattccg gcgtgaagct agctcggtcg agaatgtcgc agaaagtagc atgcaaccct
     4801 acaaagtgat ttgggaatgg aagaaggagg acgtcgaaaa aaagaagact gacttgtaaa
     4861 agggtgggcg cgccgaccca gctttcttgt acaaagtggt gacgtaagct agaggatctt
     4921 tgtgaaggaa ccttacttct gtggtgtgac ataattggac aaactaccta cagagattta
     4981 aagctctaag gtaaatataa aatttttaag tgtataatgt gttaaactac tgattctaat
     5041 tgtttgtgta ttttagattc caacctatgg aactgatgaa tgggagcagt ggtggaatgc
     5101 ctttaatgag gaaaacctgt tttgctcaga agaaatgcca tctagtgatg atgaggctac
     5161 tgctgactct caacattcta ctcctccaaa aaagaagaga aaggtagaag accccaagga
     5221 ctttccttca gaattgctaa gttttttgag tcatgctgtg tttagtaata gaactcttgc
     5281 ttgctttgct atttacacca caaaggaaaa agctgcactg ctatacaaga aaattatgga
     5341 aaaatatttg atgtatagtg ccttgactag agatcataat cagccatacc acatttgtag
     5401 aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga
     5461 atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     5521 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     5581 aactcatcaa tgtatcttat catgtctgga tccactagaa ggcctaattc tagtatgtat
     5641 gtaagttaat aaaacccatt tttgcggaaa gtagataaaa aaaacatttt ttttttttac
     5701 tgcactggat atcattgaac ttatctgatc agttttaaat ttacttcgat ccaagggtat
     5761 ttgatgtacc aggttctttc gattacctct cactcaaaat gacattccac tcaaagtcag
     5821 cgctgtttgc ctccttctct gtccacagaa atatcgccgt ctctttcgcc gctgcgtccg
     5881 ctatctcttt cgccaccgtt tgtagcgtta cgtagcgtca atgtccgcct tcagttgcat
     5941 tttgtcagcg gtttcgtgac gaagctccaa gcggtttacg ccatcaatta aacacaaagt
     6001 gctgtgccaa aactcctctc gcttcttatt tttgtttgtt ttttgagtga ttggggtggt
     6061 gattggtttt gggtgggtaa gcaggggaaa gtgtgaaaaa tcccggcaat gggccaagag
     6121 gatcaggagc tattaattcg cggaggcagc aaacacccat ctgccgagca tctgaacaat
     6181 gtgagtagta catgtgcata catcttaagt tcacttgatc tataggaact gcgattgcaa
     6241 catcaaattg tctgcggcgt gagaactgcg acccacaaaa atcccaaacc gcaattgcac
     6301 aaacaaatag tgacacgaaa cagattattc tggtagctgt tctcgctata taagacaatt
     6361 tttgagatca tatcatgatc aagacatcta aaggcattca ttttcgacta tattcttttt
     6421 tacaaaaaat ataacaacca gatattttaa gctgatccta gatgcacaaa aaataaataa
     6481 aagtataaac ctacttcgta ggatacttcg gggtactttt tgttcggggt tagatgagca
     6541 taacgcttgt agttgatatt tgagatcccc tatcattgca gggtgacagc ggagcggctt
     6601 cgcagagctg cattaaccag ggcttcgggc aggccaaaaa ctacggcacg ctccggccac
     6661 ccagtccgcc ggaggactcc ggttcaggga gcggccaact agccgagaac ctcacctatg
     6721 cctggcacaa tatggacatc tttggggcgg tcaatcagcc gggctccgga tggcggcagc
     6781 tggtcaaccg gacacgcgga ctattctgca acgagcgaca cataccggcg cccaggaaac
     6841 atttgctcaa gaacggtgag tttctattcg cagtcggctg atctgtgtga aatcttaata
     6901 aagggtccaa ttaccaattt gaaactcagt ttgcggcgtg gcctatccgg gcgaactttt
     6961 ggccgtgatg ggcagttccg gtgccggaaa gacgaccctg ctgaatgccc ttgcctttcg
     7021 atcgccgcag ggcatccaag tatcgccatc cgggatgcga ctgctcaatg gccaacctgt
     7081 ggacgccaag gagatgcagg ccaggtgcgc ctatgtccag caggatgacc tctttatcgg
     7141 ctccctaacg gccagggaac acctgatttt ccaagccatg gtgcggatgc cacgacatct
     7201 gacctatcgg cagcgagtgg cccgcgtgga tcaggtgatc caggagcttt cgctcagcaa
     7261 atgtcagcac acgatcatcg gtgtgcccgg cagggtgaaa ggtctgtccg gcggagaaag
     7321 gaagcgtctg gcattcgcct ccgaggctct aaccgatccg ccgcttctga tctgcgatga
     7381 gcccacctcc ggactggact cctttaccgc ccacagcgtc gtccaggtgc tgaagaagct
     7441 gtcgcagaag ggcaagaccg tcatcctgac cattcatcag ccgtcttccg agctgtttga
     7501 gctctttgac aagatccttc tgatggccga gggcagggta gctttcttgg gcactcccag
     7561 cgaagccgtc gacttctttt cctagtgagt tcgatgtgtt tattaagggt atctagtatt
     7621 acataacatc tcaactccta tccagcgtgg gtgcccagtg tcctaccaac tacaatccgg
     7681 cggactttta cgtacaggtg ttggccgttg tgcccggacg ggagatcgag tcccgtgatc
     7741 ggatcgccaa gatatgcgac aattttgcca ttagcaaagt agcccgggat atggagcagt
     7801 tgttggccac caaaaatctg gagaagccac tggagcagcc ggagaatggg tacacctaca
     7861 aggccacctg gttcatgcag ttccgggcgg tcctgtggcg atcctggctg tcggtgctca
     7921 aggaaccact cctcgtaaaa gtgcgactta ttcagacaac ggtgagtggt tccagtggaa
     7981 acaaatgata taacgcttac aattcttgga aacaaattcg ctagatttta gatagaattg
     8041 cctgattcca cacccttctt agtttttttc aatgagatgt atagtttata gttttgcaga
     8101 agataaataa atttcattta actcgcgaat attaatgaga tgcgagtaac attttaattt
     8161 gcagatggtt gccatcttga ttggcctcat ctttttgggc caacaactca cgcaagtggg
     8221 tgtgatgaat atcaacggag ccatcttcct cttcctgacc aacatgacct ttcaaaacgt
     8281 ctttgccacg ataaatgtaa gtcatgttta gaatacattt gcatttcaat aatttactaa
     8341 ctttctaatg aatcgattcg atttaggtgt tcacctcaga gctgccagtt tttatgaggg
     8401 aggcccgaag tcgactttat cgctgtgaca catactttct gggcaaaacg attgccgaat
     8461 tgccgctttt tctcacagtg ccactggtct tcacggcgat tgcctatccg atgatcggac
     8521 tgcgggccgg agtgctgcac ttcttcaact gcctggcgct ggtcactctg gtggccaatg
     8581 tgtcaacgtc cttcggatat ctaatatcct gcgccagctc ctcgacctcg atggcgctgt
     8641 ctgtgggtcc gccggttatc ataccattcc tgctctttgg cggcttcttc ttgaactcgg
     8701 gctcggtgcc agtatacctc aaatggttgt cgtacctctc atggttccgt tacgccaacg
     8761 agggtctgct gattaaccaa tgggcggacg tggagccggg cgaaattagc tgcacatcgt
     8821 cgaacaccac gtgccccagt tcgggcaagg tcatcctgga gacgcttaac ttctccgccg
     8881 ccgatctgcc gctggactac gtgggtctgg ccattctcat cgtgagcttc cgggtgctcg
     8941 catatctggc tctaagactt cgggcccgac gcaaggagta gccgacatat atccgaaata
     9001 actgcttgtt tttttttttt accattatta ccatcgtgtt tactgtttat tgccccctca
     9061 aaaagctaat gtaattatat ttgtgccaat aaaaacaaga tatgacctat agaatacaag
     9121 tatttcccct tcgaacatcc ccacaagtag actttggatt tgtcttctaa ccaaaagact
     9181 tacacacctg cataccttac atcaaaaact cgtttatcgc tacataaaac accgggatat
     9241 attttttata tacatacttt tcaaatcgcg cgccctcttc ataattcacc tccaccacac
     9301 cacgtttcgt agttgctctt tcgctgtctc ccacccgctc tccgcaacac attcaccttt
     9361 tgttcgacga ccttggagcg actgtcgtta gttccgcgcg attcggttcg ctcaaatggt
     9421 tccgagtggt tcatttcgtc tcaatagaaa ttagtaataa atatttgtat gtacaattta
     9481 tttgctccaa tatatttgta tatatttccc tcacagctat atttattcta atttaatatt
     9541 atgacttttt aaggtaattt tttgtgacct gttcggagtg attagcgtta caatttgaac
     9601 tgaaagtgac atccagtgtt tgttccttgt gtagatgcat ctcaaaaaaa tggtgggcat
     9661 aatagtgttg tttatatata tcaaaaataa caactataat aataagaata catttaattt
     9721 agaaaatgct tggatttcac tggaactaga attaattcgg ctgctgctct aaacgacgca
     9781 tttcgtactc caaagtacga attttttccc tcaagctctt attttcatta aacaatgaac
     9841 aggacctaac gcacagtcac gttattgttt acataaatga ttttttttac tattcaaact
     9901 tactctgttt gtgtactccc actggtatag ccttctttta tcttttctgg ttcaggctct
     9961 atcactttac taggtacggc atctgcgttg agtcgcctcc ttttaaatgt ctgacctttt
    10021 gcaggtgcag ccttccactg cgaatcatta aagtgggtat cacaaatttg ggagttttca
    10081 ccaaggctgc acccaaggct ctgctcccac aattttctct taatagcaca cttcggcacg
    10141 tgaattaatt ttactccagt cacagctttg cagcaaaatt tgcaatattt catttttttt
    10201 tattccacgt aagggttaat gttttcaaaa aaaaattcgt ccgcacacaa cctttcctct
    10261 caacaagcaa acgtgcactg aatttaagtg tatacttcgg taagcttcgg ctatcgacgg
    10321 gaccacctta tgttatttca tcatgggcca gacccacgta gtccagcggc agatcggcgg
    10381 cggagaagtt aagcgtctcc aggatgacct tgcccgaact ggggcacgtg gtgttcgacg
    10441 atgtgcagct aatttcgccc ggctccacgt ccgcccattg gttaatcagc agaccctcgt
    10501 tggcgtaacg gaaccatgag aggtacgaca accatttgag gtatactggc accgagcccg
    10561 agttcaagaa gaaggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
    10621 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
    10681 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
    10741 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
    10801 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
    10861 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
    10921 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
    10981 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
    11041 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
    11101 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
    11161 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
    11221 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
    11281 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
    11341 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
    11401 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
    11461 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
    11521 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
    11581 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
    11641 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
    11701 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
    11761 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
    11821 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
    11881 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
    11941 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
    12001 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
    12061 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
    12121 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
    12181 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
    12241 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
    12301 cgcgcacatt tccccgaaaa gtgccacctg tcgacgatgt aggtcacggt ctcgaagccg
    12361 cggtgcgggt gccagggcgt gcccttgggc tccccgggcg cgtactccac ctcacccatc
    12421 tggtccatca tgatgaacgg gtcgaggtgg cggtagttga tcccggcgaa cgcgcggcgc
    12481 accgggaagc cctcgccctc gaaaccgctg ggcgcggtg
//
