LOCUS       sequence-202525-        1902 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression plasmid to target the roGFP1-mApple sensor to 
            the mitochondrial matrix..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1902)
  AUTHORS   Norcross S, Trull KJ, Snaider J, Doan S, Tat K, Huang L, Tantama M
  TITLE     Extending roGFP Emission via Forster-Type Resonance Energy Transfer 
            Relay Enables Simultaneous Dual Compartment Ratiometric Redox 
            Imaging in Live Cells.
  JOURNAL   ACS Sens. 2017 Nov 22;2(11):1721-1729. doi: 
            10.1021/acssensors.7b00689. Epub 2017 Nov 7.
  PUBMED    29072071
REFERENCE   2  (bases 1 to 1902)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssensors.7b00689"; journalName: "ACS Sens"; date: 
            "2017-11-22- 22"; volume: "2"; issue: "11"; pages: "1721-1729"
COMMENT     Sequence Label: GW1-mito-roGFP1-mApple
FEATURES             Location/Qualifiers
     source          1..1902
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             29..103
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             125..199
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             221..295
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             317..391
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             join(446..448,449..451,452..1162)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFISTTGKLPVPWPTLVTTLSYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNCHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTCSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(491..512)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(752..771)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1099..1120
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             1184..1891
                     /codon_start=1
                     /product="photostable monomeric derivative of DsRed (Shaner
                     et al., 2008)"
                     /label=mApple
                     /note="mammalian codon-optimized"
                     /translation="VSKGEENNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEAF
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKVYIKHPADIPDYFKLSFPEGFRWERVMNFE
                     DGGIIHVNQDSSLQDGVFIYKVKLRGTNFPSDGPVMQKKTMGWEASEERMYPEDGALKS
                     EIKKRLKLKDGGHYAAEVKTTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     1594..1613
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 caggtgtgcc tgaggcctct agaccaccat gagcgtgctg acaccattgt tgctcagagg
       61 cctcactggt tcagccaggc ggctgccagt cccaagagct aagatccata gtctgggaga
      121 tccaatgtca gttctcactc cccttttgct taggggactg accggaagcg ctcggcggtt
      181 gcctgtccca cgcgccaaga ttcactccct gggcgaccct atgagtgtcc ttactcctct
      241 tttgctgaga ggcttgacag ggagcgctcg gaggctccca gtgcctcgcg caaagatcca
      301 ttctctcgga gatcccatgt ccgtcctcac tcctctgctg ctgcgcgggc ttacaggttc
      361 cgctcgccgc ctgcctgttc ctcgggcaaa gatacacagt ctgggcgatc ctcaccggcg
      421 tggaggagca tctggtggag ctagtatggt gagcaagggc gaggagctgt tcaccggggt
      481 ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg
      541 cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct ccaccaccgg
      601 caagctgccc gtgccctggc ccaccctcgt gaccactctg tcttacggcg tgcagtgctt
      661 cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg
      721 ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga
      781 ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa
      841 ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaactgcc acaacgtcta
      901 tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat
      961 cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg
     1021 ccccgtgctg ctgcccgaca accactacct gagcacctgc tccgccctga gcaaagaccc
     1081 caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct
     1141 cggcatggac gagctgtata agggaggttc aggaggtcga tccgtgagca agggcgagga
     1201 gaataacatg gccatcatca aggagttcat gcgcttcaag gtgcacatgg agggctccgt
     1261 gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg aggcctttca
     1321 gaccgctaag ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg acatcctgtc
     1381 ccctcagttc atgtacggct ccaaggtcta cattaagcac ccagccgaca tccccgacta
     1441 cttcaagctg tccttccccg agggcttcag gtgggagcgc gtgatgaact tcgaggacgg
     1501 cggcattatt cacgttaacc aggactcctc cctgcaggac ggcgtgttca tctacaaggt
     1561 gaagctgcgc ggcaccaact tcccctccga cggccccgta atgcagaaga agaccatggg
     1621 ctgggaggcc tccgaggagc ggatgtaccc cgaggacggc gccctgaaga gcgagatcaa
     1681 gaagaggctg aagctgaagg acggcggcca ctacgccgcc gaggtcaaga ccacctacaa
     1741 ggccaagaag cccgtgcagc tgcccggcgc ctacatcgtc gacatcaagt tggacatcgt
     1801 gtcccacaac gaggactaca ccatcgtgga acagtacgaa cgcgccgagg gtcgtcactc
     1861 taccggcggc atggacgagc tgtacaagta ataaactcga cg
//
