LOCUS       sequence-202527-        1908 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression plasmid to target the mCherry-roGFP2 sensor to 
            the mitochondrial matrix..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1908)
  AUTHORS   Norcross S, Trull KJ, Snaider J, Doan S, Tat K, Huang L, Tantama M
  TITLE     Extending roGFP Emission via Forster-Type Resonance Energy Transfer 
            Relay Enables Simultaneous Dual Compartment Ratiometric Redox 
            Imaging in Live Cells.
  JOURNAL   ACS Sens. 2017 Nov 22;2(11):1721-1729. doi: 
            10.1021/acssensors.7b00689. Epub 2017 Nov 7.
  PUBMED    29072071
REFERENCE   2  (bases 1 to 1908)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssensors.7b00689"; journalName: "ACS Sens"; date: 
            "2017-11-22- 22"; volume: "2"; issue: "11"; pages: "1721-1729"
COMMENT     Sequence Label: GW1-mito-mCherry-roGFP2
FEATURES             Location/Qualifiers
     source          1..1908
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             38..112
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             134..208
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             230..304
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             326..400
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             455..1162
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(600..618)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     868..887
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             join(1184..1186,1187..1900)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FISTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNCHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTCSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(1226..1247)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1487..1506)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1834..1855
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 tcggcggagc aggtgtgcct gaggcctcta gaccaccatg agcgtgctga caccattgtt
       61 gctcagaggc ctcactggtt cagccaggcg gctgccagtc ccaagagcta agatccatag
      121 tctgggagat ccaatgtcag ttctcactcc ccttttgctt aggggactga ccggaagcgc
      181 tcggcggttg cctgtcccac gcgccaagat tcactccctg ggcgacccta tgagtgtcct
      241 tactcctctt ttgctgagag gcttgacagg gagcgctcgg aggctcccag tgcctcgcgc
      301 aaagatccat tctctcggag atcccatgtc cgtcctcact cctctgctgc tgcgcgggct
      361 tacaggttcc gctcgccgcc tgcctgttcc tcgggcaaag atacacagtc tgggcgatcc
      421 tcaccggcgt ggaggagcat ctggtggagc tagtatggtg agcaagggcg aggaggataa
      481 catggccatc atcaaggagt tcatgcgctt caaggtgcac atggagggct ccgtgaacgg
      541 ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc
      601 caagctgaag gtgaccaagg gtggccccct gcccttcgcc tgggacatcc tgtcccctca
      661 gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc gacatccccg actacttgaa
      721 gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt
      781 ggtgaccgtg acccaggact cctccctgca ggacggcgag ttcatctaca aggtgaagct
      841 gcgcggcacc aacttcccct ccgacggccc cgtaatgcag aagaagacca tgggctggga
      901 ggcctcctcc gagcggatgt accccgagga cggcgccctg aagggcgaga tcaagcagag
      961 gctgaagctg aaggacggcg gccactacga cgctgaggtc aagaccacct acaaggccaa
     1021 gaagcccgtg cagctgcccg gcgcctacaa cgtcaacatc aagttggaca tcacctccca
     1081 caacgaggac tacaccatcg tggaacagta cgaacgcgcc gagggtcgtc actctaccgg
     1141 cggcatggac gagctgtaca agggaggttc aggaggtcga tccgtgagca agggcgagga
     1201 gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa
     1261 gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt
     1321 catctccacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta
     1381 cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc
     1441 cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta
     1501 caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa
     1561 gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa
     1621 ctgccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa
     1681 gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac
     1741 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca cctgctccgc
     1801 cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc
     1861 cgccgggatc actctcggca tggacgagct gtacaagtaa taaactcg
//
