LOCUS       sequence-20253-e         732 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 732)
  AUTHORS   Yuan YR, Pei Y, Ma JB, Kuryavyi V, Zhadina M, Meister G, Chen HY, 
            Dauter Z, Tuschl T, Patel DJ
  TITLE     Crystal structure of A. aeolicus argonaute, a site-specific 
            DNA-guided endoribonuclease, provides insights into RISC-mediated 
            mRNA cleavage.
  JOURNAL   Mol Cell. 2005 Aug 5. 19(3):405-19.
  PUBMED    16061186
REFERENCE   2  (bases 1 to 732)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2005 Aug 5. 19(3):405-19."
COMMENT     Sequence Label: pDESTFLAGHApuro3-Ago Chi-a
FEATURES             Location/Qualifiers
     source          1..732
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      37..46
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             46..69
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             70..96
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    112..136
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gatccatccg atagtggtgg tggaattctg atcgccgccg ccatggacta caaggacgac
       61 gatgacaagt acccttatga cgtgcccgat tacgctttga accagatatc aacaagtttg
      121 tacaaaaaag caggctccac catgggaacc aattcagtcg actggatccg gatggaagcg
      181 ggaccctcgg gagcagctgc gggcgcttac ctgccccccc tgcagcaggt gttccaggca
      241 cctcgccggc ctggcattgg cactgtgggg aaaccaatca agctcctggc caattacttt
      301 gaggtggaca tccctaagat cgacgtgtac cactacgagg tggacatcaa gccggataag
      361 tgtccccgta gagtcaaccg ggaagtggtg gaatacatgg tccagcattt caagcctcag
      421 atctttggtg atcgcaagcc tgtgtatgat ggaaagaaga acatttacac tgtcacagca
      481 ctgcccattg gcaacgaacg ggtcgacttt gaggtgacaa tccctgggga agggaaggat
      541 cgaatcttta aggtctccat caagtggcta gccattgtga gctggcgaat gctgcatgag
      601 gccctggtca gcggccagat ccctgttccc ttggagtctg tgcaagccct ggatgtggcc
      661 atgaggcacc tggcatccat gaggtacacc cctgtgggcc gctccttctt ctcaccgcct
      721 gagggctact ac
//
