LOCUS       sequence-202532-        9467 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses humanized AsCpf1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9467)
  AUTHORS   Zetsche B, Gootenberg JS, Abudayyeh OO, Slaymaker IM, Makarova KS, 
            Essletzbichler P, Volz SE, Joung J, van der Oost J, Regev A, Koonin 
            EV, Zhang F
  TITLE     Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas 
            System.
  JOURNAL   Cell. 2015 Sep 23. pii: S0092-8674(15)01200-3. doi: 
            10.1016/j.cell.2015.09.038.
  PUBMED    26422227
REFERENCE   2  (bases 1 to 9467)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2015 
            Sep 23. pii: S0092-8674(15)01200-3. doi: 
            10.1016/j.cell.2015.09.038."
COMMENT     Sequence Label: pY010 (pcDNA3.1-hAsCpf1)
FEATURES             Location/Qualifiers
     source          1..9467
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             929..4849
                     /codon_start=1
                     /product="CRISPR-associated protein from Acidaminococcus 
                     sp. BV3L6 (Zetsche et al., 2015)"
                     /label=AsCpf1
                     /translation="MTQFEGFTNLYQVSKTLRFELIPQGKTLKHIQEQGFIEEDKARND
                     HYKELKPIIDRIYKTYADQCLQLVQLDWENLSAAIDSYRKEKTEETRNALIEEQATYRN
                     AIHDYFIGRTDNLTDAINKRHAEIYKGLFKAELFNGKVLKQLGTVTTTEHENALLRSFD
                     KFTTYFSGFYENRKNVFSAEDISTAIPHRIVQDNFPKFKENCHIFTRLITAVPSLREHF
                     ENVKKAIGIFVSTSIEEVFSFPFYNQLLTQTQIDLYNQLLGGISREAGTEKIKGLNEVL
                     NLAIQKNDETAHIIASLPHRFIPLFKQILSDRNTLSFILEEFKSDEEVIQSFCKYKTLL
                     RNENVLETAEALFNELNSIDLTHIFISHKKLETISSALCDHWDTLRNALYERRISELTG
                     KITKSAKEKVQRSLKHEDINLQEIISAAGKELSEAFKQKTSEILSHAHAALDQPLPTTL
                     KKQEEKEILKSQLDSLLGLYHLLDWFAVDESNEVDPEFSARLTGIKLEMEPSLSFYNKA
                     RNYATKKPYSVEKFKLNFQMPTLASGWDVNKEKNNGAILFVKNGLYYLGIMPKQKGRYK
                     ALSFEPTEKTSEGFDKMYYDYFPDAAKMIPKCSTQLKAVTAHFQTHTTPILLSNNFIEP
                     LEITKEIYDLNNPEKEPKKFQTAYAKKTGDQKGYREALCKWIDFTRDFLSKYTKTTSID
                     LSSLRPSSQYKDLGEYYAELNPLLYHISFQRIAEKEIMDAVETGKLYLFQIYNKDFAKG
                     HHGKPNLHTLYWTGLFSPENLAKTSIKLNGQAELFYRPKSRMKRMAHRLGEKMLNKKLK
                     DQKTPIPDTLYQELYDYVNHRLSHDLSDEARALLPNVITKEVSHEIIKDRRFTSDKFFF
                     HVPITLNYQAANSPSKFNQRVNAYLKEHPETPIIGIDRGERNLIYITVIDSTGKILEQR
                     SLNTIQQFDYQKKLDNREKERVAARQAWSVVGTIKDLKQGYLSQVIHEIVDLMIHYQAV
                     VVLENLNFGFKSKRTGIAEKAVYQQFEKMLIDKLNCLVLKDYPAEKVGGVLNPYQLTDQ
                     FTSFAKMGTQSGFLFYVPAPYTSKIDPLTGFVDPFVWKTIKNHESRKHFLEGFDFLHYD
                     VKTGDFILHFKMNRNLSFQRGLPGFMPAWDIVFEKNETQFDAKGTPFIAGKRIVPVIEN
                     HRFTGRYRDLYPANELIALLEEKGIVFRDGSNILPKLLENDDSHAIDTMVALIRSVLQM
                     RNSNAATGEDYINSPVRDLNGVCFDSRFQNPEWPMDADANGAYHIALKGQLLLNHLKES
                     KDLKLQNGISNQDWLAYIQELRN"
     regulatory      4569..4578
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4850..4897
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             4904..4984
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     primer_bind     complement(5058..5075)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5064..5288
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5334..5762
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5421..5440)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5631..5652
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5771..5791)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5776..6105
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5956..6091
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6018..6037
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6172..6966
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6226..6245)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6836..6855
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7140..7261
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7177..7196)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7231..7250
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7310..7326)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7310..7326)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7323..7345)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7334..7350
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7358..7364,7365..7382,7383..7388))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7403..7424
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7541..7558)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7712..8300)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7792..7811)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8471..9262,9263..9331))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9094..9113
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9332..9436)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttggta ccgccaccat gacacagttc gagggcttta ccaacctgta
      961 tcaggtgagc aagacactgc ggtttgagct gatcccacag ggcaagaccc tgaagcacat
     1021 ccaggagcag ggcttcatcg aggaggacaa ggcccgcaat gatcactaca aggagctgaa
     1081 gcccatcatc gatcggatct acaagaccta tgccgaccag tgcctgcagc tggtgcagct
     1141 ggattgggag aacctgagcg ccgccatcga ctcctataga aaggagaaaa ccgaggagac
     1201 aaggaacgcc ctgatcgagg agcaggccac atatcgcaat gccatccacg actacttcat
     1261 cggccggaca gacaacctga ccgatgccat caataagaga cacgccgaga tctacaaggg
     1321 cctgttcaag gccgagctgt ttaatggcaa ggtgctgaag cagctgggca ccgtgaccac
     1381 aaccgagcac gagaacgccc tgctgcggag cttcgacaag tttacaacct acttctccgg
     1441 cttttatgag aacaggaaga acgtgttcag cgccgaggat atcagcacag ccatcccaca
     1501 ccgcatcgtg caggacaact tccccaagtt taaggagaat tgtcacatct tcacacgcct
     1561 gatcaccgcc gtgcccagcc tgcgggagca ctttgagaac gtgaagaagg ccatcggcat
     1621 cttcgtgagc acctccatcg aggaggtgtt ttccttccct ttttataacc agctgctgac
     1681 acagacccag atcgacctgt ataaccagct gctgggagga atctctcggg aggcaggcac
     1741 cgagaagatc aagggcctga acgaggtgct gaatctggcc atccagaaga atgatgagac
     1801 agcccacatc atcgcctccc tgccacacag attcatcccc ctgtttaagc agatcctgtc
     1861 cgataggaac accctgtctt tcatcctgga ggagtttaag agcgacgagg aagtgatcca
     1921 gtccttctgc aagtacaaga cactgctgag aaacgagaac gtgctggaga cagccgaggc
     1981 cctgtttaac gagctgaaca gcatcgacct gacacacatc ttcatcagcc acaagaagct
     2041 ggagacaatc agcagcgccc tgtgcgacca ctgggataca ctgaggaatg ccctgtatga
     2101 gcggagaatc tccgagctga caggcaagat caccaagtct gccaaggaga aggtgcagcg
     2161 cagcctgaag cacgaggata tcaacctgca ggagatcatc tctgccgcag gcaaggagct
     2221 gagcgaggcc ttcaagcaga aaaccagcga gatcctgtcc cacgcacacg ccgccctgga
     2281 tcagccactg cctacaaccc tgaagaagca ggaggagaag gagatcctga agtctcagct
     2341 ggacagcctg ctgggcctgt accacctgct ggactggttt gccgtggatg agtccaacga
     2401 ggtggacccc gagttctctg cccggctgac cggcatcaag ctggagatgg agccttctct
     2461 gagcttctac aacaaggcca gaaattatgc caccaagaag ccctactccg tggagaagtt
     2521 caagctgaac tttcagatgc ctacactggc ctctggctgg gacgtgaata aggagaagaa
     2581 caatggcgcc atcctgtttg tgaagaacgg cctgtactat ctgggcatca tgccaaagca
     2641 gaagggcagg tataaggccc tgagcttcga gcccacagag aaaaccagcg agggctttga
     2701 taagatgtac tatgactact tccctgatgc cgccaagatg atcccaaagt gcagcaccca
     2761 gctgaaggcc gtgacagccc actttcagac ccacacaacc cccatcctgc tgtccaacaa
     2821 tttcatcgag cctctggaga tcacaaagga gatctacgac ctgaacaatc ctgagaagga
     2881 gccaaagaag tttcagacag cctacgccaa gaaaaccggc gaccagaagg gctacagaga
     2941 ggccctgtgc aagtggatcg acttcacaag ggattttctg tccaagtata ccaagacaac
     3001 ctctatcgat ctgtctagcc tgcggccatc ctctcagtat aaggacctgg gcgagtacta
     3061 tgccgagctg aatcccctgc tgtaccacat cagcttccag agaatcgccg agaaggagat
     3121 catggatgcc gtggagacag gcaagctgta cctgttccag atctataaca aggactttgc
     3181 caagggccac cacggcaagc ctaatctgca cacactgtat tggaccggcc tgttttctcc
     3241 agagaacctg gccaagacaa gcatcaagct gaatggccag gccgagctgt tctaccgccc
     3301 taagtccagg atgaagagga tggcacaccg gctgggagag aagatgctga acaagaagct
     3361 gaaggatcag aaaaccccaa tccccgacac cctgtaccag gagctgtacg actatgtgaa
     3421 tcacagactg tcccacgacc tgtctgatga ggccagggcc ctgctgccca acgtgatcac
     3481 caaggaggtg tctcacgaga tcatcaagga taggcgcttt accagcgaca agttcttttt
     3541 ccacgtgcct atcacactga actatcaggc cgccaattcc ccatctaagt tcaaccagag
     3601 ggtgaatgcc tacctgaagg agcaccccga gacacctatc atcggcatcg atcggggcga
     3661 gagaaacctg atctatatca cagtgatcga ctccaccggc aagatcctgg agcagcggag
     3721 cctgaacacc atccagcagt ttgattacca gaagaagctg gacaacaggg agaaggagag
     3781 ggtggcagca aggcaggcct ggtctgtggt gggcacaatc aaggatctga agcagggcta
     3841 tctgagccag gtcatccacg agatcgtgga cctgatgatc cactaccagg ccgtggtggt
     3901 gctggagaac ctgaatttcg gctttaagag caagaggacc ggcatcgccg agaaggccgt
     3961 gtaccagcag ttcgagaaga tgctgatcga taagctgaat tgcctggtgc tgaaggacta
     4021 tccagcagag aaagtgggag gcgtgctgaa cccataccag ctgacagacc agttcacctc
     4081 ctttgccaag atgggcaccc agtctggctt cctgttttac gtgcctgccc catatacatc
     4141 taagatcgat cccctgaccg gcttcgtgga ccccttcgtg tggaaaacca tcaagaatca
     4201 cgagagccgc aagcacttcc tggagggctt cgactttctg cactacgacg tgaaaaccgg
     4261 cgacttcatc ctgcacttta agatgaacag aaatctgtcc ttccagaggg gcctgcccgg
     4321 ctttatgcct gcatgggata tcgtgttcga gaagaacgag acacagtttg acgccaaggg
     4381 cacccctttc atcgccggca agagaatcgt gccagtgatc gagaatcaca gattcaccgg
     4441 cagataccgg gacctgtatc ctgccaacga gctgatcgcc ctgctggagg agaagggcat
     4501 cgtgttcagg gatggctcca acatcctgcc aaagctgctg gagaatgacg attctcacgc
     4561 catcgacacc atggtggccc tgatccgcag cgtgctgcag atgcggaact ccaatgccgc
     4621 cacaggcgag gactatatca acagccccgt gcgcgatctg aatggcgtgt gcttcgactc
     4681 ccggtttcag aacccagagt ggcccatgga cgccgatgcc aatggcgcct accacatcgc
     4741 cctgaagggc cagctgctgc tgaatcacct gaaggagagc aaggatctga agctgcagaa
     4801 cggcatctcc aatcaggact ggctggccta catccaggag ctgcgcaaca aaaggccggc
     4861 ggccacgaaa aaggccggcc aggcaaaaaa gaaaaaggga tcctacccat acgatgttcc
     4921 agattacgct tatccctacg acgtgcctga ttatgcatac ccatatgatg tccccgacta
     4981 tgcctaagaa ttctgcagat atccagcaca gtggcggccg ctcgagtcta gagggcccgt
     5041 ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc
     5101 ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa
     5161 tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg
     5221 gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg
     5281 ctctatggct tctgaggcgg aaagaaccag ctggggctct agggggtatc cccacgcgcc
     5341 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     5401 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     5461 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     5521 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
     5581 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     5641 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
     5701 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     5761 ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc
     5821 agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc
     5881 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg
     5941 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     6001 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc tgagctattc
     6061 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct cccgggagct
     6121 tgtatatcca ttttcggatc tgatcaagag acaggatgag gatcgtttcg catgattgaa
     6181 caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac
     6241 tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg
     6301 cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaggacgag
     6361 gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt
     6421 gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg
     6481 tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg
     6541 catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga
     6601 gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag
     6661 gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat
     6721 ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt
     6781 tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg
     6841 gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt
     6901 tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc
     6961 ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac
     7021 gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg
     7081 acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca
     7141 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     7201 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     7261 atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt
     7321 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     7381 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     7441 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     7501 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     7561 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     7621 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     7681 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     7741 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     7801 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     7861 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     7921 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     7981 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     8041 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     8101 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     8161 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     8221 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     8281 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     8341 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     8401 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     8461 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     8521 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     8581 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     8641 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     8701 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     8761 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     8821 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     8881 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     8941 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     9001 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     9061 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     9121 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     9181 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     9241 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     9301 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     9361 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     9421 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtc
//
