LOCUS       sequence-20254-e         668 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 668)
  AUTHORS   Yuan YR, Pei Y, Ma JB, Kuryavyi V, Zhadina M, Meister G, Chen HY, 
            Dauter Z, Tuschl T, Patel DJ
  TITLE     Crystal structure of A. aeolicus argonaute, a site-specific 
            DNA-guided endoribonuclease, provides insights into RISC-mediated 
            mRNA cleavage.
  JOURNAL   Mol Cell. 2005 Aug 5. 19(3):405-19.
  PUBMED    16061186
REFERENCE   2  (bases 1 to 668)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2005 Aug 5. 19(3):405-19."
COMMENT     Sequence Label: pDESTFLAGHApuro3-Ago Chi-b
FEATURES             Location/Qualifiers
     source          1..668
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             9..23
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
     CDS             24..50
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    66..90
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 actacaagga cgacgatgac aagtaccctt atgacgtgcc cgattacgct ttgaaccaga
       61 tatcaacaag tttgtacaaa aaagcaggct ccaccatggg aaccaattca gtcgactgga
      121 tccggatgta ctcgggagcc ggccccgcac ttgcacctcc tgcgccgccg ccccccatcc
      181 aaggatatgc cttcaagcct ccacctagac ccgactttgg gacctccggg agaacaatca
      241 aattacaggc caatttcttc gaaatggaca tccccaaaat tgacatctat cattatgaat
      301 tggatatcaa gccagagaag tgcccgagga gagttaacag ggaaatcgtg gaacacatgg
      361 tccagcactt taaaacacag atctttgggg atcggaagcc cgtgtttgac ggcaggaaga
      421 atctatacac agccatgccc cttccgattg ggagggacaa ggtggagctg gaggtcacgc
      481 tgccaggaga aggcaaggat cgcatcttca aggtgtccat caagtgggtg tcctgcgtga
      541 gcttgcaggc gttacacgat gcactttcag ggcggctgcc cagcgtccct tttgagacga
      601 tccaggccct ggacgtggtc atgaggcact tgccatccat gaggtacacc cccgtgggcc
      661 gctccttc
//
