LOCUS       sequence-20255-e         388 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 388)
  AUTHORS   Yuan YR, Pei Y, Ma JB, Kuryavyi V, Zhadina M, Meister G, Chen HY, 
            Dauter Z, Tuschl T, Patel DJ
  TITLE     Crystal structure of A. aeolicus argonaute, a site-specific 
            DNA-guided endoribonuclease, provides insights into RISC-mediated 
            mRNA cleavage.
  JOURNAL   Mol Cell. 2005 Aug 5. 19(3):405-19.
  PUBMED    16061186
REFERENCE   2  (bases 1 to 388)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2005 Aug 5. 19(3):405-19."
COMMENT     Sequence Label: pDESTFLAGHApuro3-Ago Chi-c
FEATURES             Location/Qualifiers
     source          1..388
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      36..45
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             45..68
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             69..95
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    111..135
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 cgatccatcg atagtgtggt ggaattctga tcgccgccgc catggactac aaggacgacg
       61 atgacaagta cccttatgac gtgcccgatt acgctttgaa ccagatatca acaagtttgt
      121 acaaaaaagc aggctccacc atgggaacca attcagtcga ctggatccgg atggaagcgg
      181 gaccctcggg agcagctgcg ggcgcttacc tgccccccct gcagcaggtg ttccaggcac
      241 ctcgccggcc tggcattggc actgtgggga aaccaatcaa gctcctggcc aattactttg
      301 aggtggacat ccccaagatc gacgtgtacc actacgaggt ggacatcaag ccggataagt
      361 gtccccgtag agtcaaccgg gaagtggt
//
