LOCUS       sequence-202553-       10148 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Nd1 and puromycin resistent gene under control of TetON 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10148)
  AUTHORS   Zhang Y, Pak C, Han Y, Ahlenius H, Zhang Z, Chanda S, Marro S, 
            Patzke C, Acuna C, Covy J, Xu W, Yang N, Danko T, Chen L, Wernig M, 
            Sudhof TC
  TITLE     Rapid single-step induction of functional neurons from human 
            pluripotent stem cells.
  JOURNAL   Neuron. 2013 Jun 5;78(5):785-98. doi: 10.1016/j.neuron.2013.05.029.
  PUBMED    23764284
REFERENCE   2  (bases 1 to 10148)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2013.05"; journalName: "Neuron"; date: "2013-06-5-
            5"; volume: "78"; issue: "5"; pages: "785-98"
COMMENT     Sequence Label: pTet-O-Nd1-puro
FEATURES             Location/Qualifiers
     source          1..10148
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1094..1147
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             1154..1753
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(1154..1173)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     1650..1670
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    1792..2380
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1845..1865)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2263..2274)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2383..2399)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2384..2400)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             2905..3085
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(3111..3128)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3117..3341
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3387..3815
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3474..3493)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3684..3705
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3824..3844)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3829..4158
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4009..4144
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4071..4090
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        4206..4253
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             4272..4646
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    4776..4897
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4813..4832)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4867..4886
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4946..4962)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4946..4962)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4959..4981)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4970..4986
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4994..5000,5001..5018,5019..5024))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5039..5060
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5177..5194)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5348..5936)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5428..5447)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6107..6898,6899..6967))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6730..6749
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6968..7072)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7147..7166)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        7338..7717
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7719..7917
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             7935..8115
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8162..8287
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8780..9013
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9198..9242
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9540..9657
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(9714..9732,9733..9755,9756..9774,9775..9797,9798..9816,
                     9817..9839,9840..9858,9859..9881,9882..9900,9901..9923,
                     9924..9942,9943..9965,9966..9984)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        10017..10055
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     10052..10076
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 gaattcgcta gccaccatga ccaaatcata cagcgagagc gggctgatgg gcgagcctca
       61 gccccaaggt cccccaagct ggacagatga gtgtctcagt tctcaggacg aggaacacga
      121 ggcagacaag aaagaggacg agcttgaagc catgaatgca gaggaggact ctctgagaaa
      181 cgggggagag gaggaggagg aagatgagga tctagaggaa gaggaggaag aagaagagga
      241 ggaggaggat caaaagccca agagacgggg tcccaaaaag aaaaagatga ccaaggcgcg
      301 cctagaacgt tttaaattaa ggcgcatgaa ggccaacgcc cgcgagcgga accgcatgca
      361 cgggctgaac gcggcgctgg acaacctgcg caaggtggta ccttgctact ccaagaccca
      421 gaaactgtct aaaatagaga cactgcgctt ggccaagaac tacatctggg ctctgtcaga
      481 gatcctgcgc tcaggcaaaa gccctgatct ggtctccttc gtacagacgc tctgcaaagg
      541 tttgtcccag cccactacca atttggtcgc cggctgcctg cagctcaacc ctcggacttt
      601 cttgcctgag cagaacccgg acatgccccc gcatctgcca accgccagcg cttccttccc
      661 ggtgcatccc tactcctacc agtcccctgg actgcccagc ccgccctacg gcaccatgga
      721 cagctcccac gtcttccacg tcaagccgcc gccacacgcc tacagcgcag ctctggagcc
      781 cttctttgaa agccccctaa ctgactgcac cagcccttcc tttgacggac ccctcagccc
      841 gccgctcagc atcaatggca acttctcttt caaacacgaa ccatccgccg agtttgaaaa
      901 aaattatgcc tttaccatgc actaccctgc agcgacgctg gcagggcccc aaagccacgg
      961 atcaatcttc tcttccggtg ccgctgcccc tcgctgcgag atccccatag acaacattat
     1021 gtctttcgat agccattcgc atcatgagcg agtcatgagt gcccagctta atgccatctt
     1081 tcacgatact agagagggca ggggaagtct tctaacatgc ggggacgtgg aggaaaatcc
     1141 cggcccactc gagatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt
     1201 ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac
     1261 cgtcgatccg gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg
     1321 cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg
     1381 gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc
     1441 cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca
     1501 ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg
     1561 caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt
     1621 gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt
     1681 caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa
     1741 gcccggtgcc tgattaatta agctagctaa ttcgatatca agcttatcga taatcaacct
     1801 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
     1861 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
     1921 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
     1981 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
     2041 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
     2101 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
     2161 gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt
     2221 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
     2281 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
     2341 cctcagacga gtcggatctc cctttgggcc gcctccccgc atcgataccg tcgacctcga
     2401 gacctagaaa aacatggagc aatcacaagt agcaatacag cagctaccaa tgctgattgt
     2461 gcctggctag aagcacaaga ggaggaggag gtgggttttc cagtcacacc tcaggtacct
     2521 ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg
     2581 ggactggaag ggctaattca ctcccaacga agacaagata tccttgatct gtggatctac
     2641 cacacacaag gctacttccc tgattggcag aactacacac cagggccagg gatcagatat
     2701 ccactgacct ttggatggtg ctacaagcta gtaccagttg agcaagagaa ggtagaagaa
     2761 gccaatgaag gagagaacac ccgcttgtta caccctgtga gcctgcatgg gatggatgac
     2821 ccggagagag aagtattaga gtggaggttt gacagccgcc tagcatttca tcacatggcc
     2881 cgagagctgc atccggactg tactgggtct ctctggttag accagatctg agcctgggag
     2941 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     3001 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     3061 tagtcagtgt ggaaaatctc tagcagggcc cgtttaaacc cgctgatcag cctcgactgt
     3121 gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga
     3181 aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag
     3241 taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga
     3301 agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac
     3361 cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg
     3421 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     3481 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     3541 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     3601 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     3661 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
     3721 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
     3781 aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt gtgtcagtta
     3841 gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     3901 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     3961 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
     4021 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     4081 gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     4141 ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg atctgatcag
     4201 cacgtgttga caattaatca tcggcatagt atatcggcat agtataatac gacaaggtga
     4261 ggaactaaac catggccaag ttgaccagtg ccgttccggt gctcaccgcg cgcgacgtcg
     4321 ccggagcggt cgagttctgg accgaccggc tcgggttctc ccgggacttc gtggaggacg
     4381 acttcgccgg tgtggtccgg gacgacgtga ccctgttcat cagcgcggtc caggaccagg
     4441 tggtgccgga caacaccctg gcctgggtgt gggtgcgcgg cctggacgag ctgtacgccg
     4501 agtggtcgga ggtcgtgtcc acgaacttcc gggacgcctc cgggccggcc atgaccgaga
     4561 tcggcgagca gccgtggggg cgggagttcg ccctgcgcga cccggccggc aactgcgtgc
     4621 acttcgtggc cgaggagcag gactgacacg tgctacgaga tttcgattcc accgccgcct
     4681 tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc
     4741 gcggggatct catgctggag ttcttcgccc accccaactt gtttattgca gcttataatg
     4801 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     4861 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgtata ccgtcgacct
     4921 ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     4981 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     5041 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     5101 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     5161 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     5221 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     5281 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     5341 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     5401 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     5461 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     5521 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     5581 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     5641 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     5701 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     5761 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     5821 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     5881 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     5941 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     6001 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     6061 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     6121 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     6181 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     6241 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     6301 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     6361 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     6421 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     6481 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     6541 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     6601 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     6661 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     6721 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     6781 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     6841 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     6901 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     6961 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     7021 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     7081 cccgaaaagt gccacctgac gtcgacggat cgggagatct cccgatcccc tatggtgcac
     7141 tctcagtaca atctgctctg atgccgcata gttaagccag tatctgctcc ctgcttgtgt
     7201 gttggaggtc gctgagtagt gcgcgagcaa aatttaagct acaacaaggc aaggcttgac
     7261 cgacaattgc atgaagaatc tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg
     7321 ggccagatat acgcgttgac attgattatt gactagttat taatagtaat caattacggg
     7381 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
     7441 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
     7501 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
     7561 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga
     7621 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg
     7681 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat
     7741 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
     7801 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc
     7861 cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagcgc
     7921 gttttgcctg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
     7981 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     8041 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     8101 ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga
     8161 gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg
     8221 actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga
     8281 gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc
     8341 agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg
     8401 attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca
     8461 gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc
     8521 aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa
     8581 gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg ctgatcttca
     8641 gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa tataaagtag
     8701 taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag
     8761 aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca
     8821 ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag
     8881 tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca
     8941 cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg
     9001 atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc
     9061 cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga
     9121 tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat
     9181 cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt
     9241 tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag
     9301 taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta
     9361 ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccgaca
     9421 ggcccgaagg aatagaagaa gaaggtggag agagagacag agacagatcc attcgattag
     9481 tgaacggatc ggcactgcgt gcgccaattc tgcagacaaa tggcagtatt catccacaat
     9541 tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata
     9601 gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttcgg
     9661 gtttattaca gggacagcag agatccagtt tggttagatc tcgagtttac cactccctat
     9721 cagtgataga gaaaagtgaa agtcgagttt accactccct atcagtgata gagaaaagtg
     9781 aaagtcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gtttaccact
     9841 ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga
     9901 aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa agtcgagttt
     9961 accactccct atcagtgata gagaaaagtg aaagtcgagc tcggtacccg ggtcgaggta
    10021 ggcgtgtacg gtgggaggcc tatataagca gagctcgttt agtgaaccgt cagatcgcct
    10081 ggagacgcca tccacgctgt tttgacctcc atagaagaca ccgggaccga tccagcctcc
    10141 gcggcccc
//
