LOCUS       sequence-202554-       10024 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Yeast Expression Plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10024)
  AUTHORS   Stewart-Ornstein J, Chen S, Bhatnagar R, Weissman JS, El-Samad H
  TITLE     Model-guided optogenetic study of PKA signaling in budding yeast.
  JOURNAL   Mol Biol Cell. 2017 Jan 1;28(1):221-227. doi: 
            10.1091/mbc.E16-06-0354. Epub 2016 Nov 9.
  PUBMED    28035051
REFERENCE   2  (bases 1 to 10024)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1091/mbc.E16-06-0354"; journalName: "Mol Biol Cell"; date: 
            "2017-01-1- 1"; volume: "28"; issue: "1"; pages: "221-227"
COMMENT     Sequence Label: pHES262  [prADH1-Msn2-mCherry, pnh604]
FEATURES             Location/Qualifiers
     source          1..10024
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        2205..2606
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     CDS             4761..5462
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="SKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGTQ
                     TAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFED
                     GGVATVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGE
                     IKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAEG
                     RHSTGGMDELYK"
     primer_bind     complement(4900..4918)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     5168..5187
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(6571..6590)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6572..6590)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6597..6614)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6597..6613)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6606..6628)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6754..7209
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6841..6860)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7051..7072
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7565..7584)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7684..7706
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7744..7762)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7847..7951
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7952..8020,8021..8812)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8170..8189)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8983..9571
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9472..9491
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9725..9742
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9859..9880
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9895..9900,9901..9918,9919..9925)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9933..9949
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9938..9960
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9957..9973
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9957..9973
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        9994..10012
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ggccggccat ttaaatgttt aaacggccac gctggccata tttctaccaa tctctcaaca
       61 ctgagtaatg gtagttataa gaaagagacc gagttaggga cagttagagg cggtggagat
      121 attccttatg gcatgtctgg cgatgataaa acttttcaaa cggcagcccc gatctaaaag
      181 agctgacagg gaaatggtca gaaaaagaaa cgtgcacccg cccgtctgga cgcgccgctc
      241 acccgcacgg cagagaccaa tcagtaaaaa tcaacggtta acgacattac tatatatata
      301 atataggaag catttaatag aacagcatcg taatatatgt gtactttgca gttatgacgc
      361 cagatggcag tagtggaaga tattctttat tgaaaaatag cttgtcacct tacgtacaat
      421 cttgatccgg agcccgtcgc tttgagagaa atcagaagat tccaaaagtc tactgaactt
      481 ttgatcagaa agttgccttt ccaaagacta gtcagagaaa tcgcccaaga tttcaagacc
      541 gatctaagat tccaatcctc tgccatcggt gctctacagg aatccgttga agcttacttg
      601 gtctctttgt tcgaagacac caacttggcc gctatccacg ctaagcgtgt taccatccaa
      661 aagaaggata tcaagttggc tagaagattg agaggtgaaa gatcctaagc acacagggat
      721 tgttgtacaa ttacttttta tgattttaga tacacacaaa tcttttctac gattaccttt
      781 catttatttt cgtttcatgt tttattaatc attctttttg tatatttcac atcgggtggg
      841 gtttttatat taaattttaa taacataatc ttttaaagtt tctactttct atcctgtata
      901 ttatttccat atctatatct atattttcaa gcttgttcac atccttatcg gaattgttcc
      961 agcgtttttg tacagaaaat gatgttatgg taacgagcta tatcagacct actagtctta
     1021 tagagctgta cagagtcttg gaaacataac caacacacca acaagatgtc atttgattcg
     1081 ttactcgaca agaatgataa gctggtaaaa gtttgcggga ttcaaaccgt cgaggctgcc
     1141 gaaactgcgc ttcaagcagg cgctgatttg atagggatca tatgtgtccc caacaggaag
     1201 cggactatcg agagcgctgt ggctcgtgaa atatccaaat tgattcacaa atcaggtact
     1261 acaaagctgg tgggggtgtt caggaatcaa tctgttgagg acgtacatcg gctttctgag
     1321 gaatatgacc ttgacataat ccaattacat ggtgatgaat catggccaga gtactataac
     1381 gtcattaaga aaccaataat caaaagagtc atattcccta gagatgtcga tgttgtaaca
     1441 caagtgtgtc aaagaaaacc cttggtatgt ctaccattgt tcgactccga ggcaggcggt
     1501 acaggtgaaa agctggactg gtccagtatt tcctcctggg caagcgaaca gaataacgta
     1561 ttttatatac tagcgggtgg actaactgca gaaaacgtca tggaggcggt ggggttgcct
     1621 ggtgtaattg gtgttgacgt ctcaggtggt gtagagactg acggcgtcaa agacaatgac
     1681 aagataatta aatatgtaca aaatgcaaag aaacaatgaa ttacgaagag aagtctcaat
     1741 gaggttttac aaacaatcct ataatacata tatgccactt agatatactt tgaatactga
     1801 atatactgac tcattatatt ctttaattgt agtttctcaa aaatattcat acaggttagc
     1861 tatgaattca tgctataatt tccattttgt tcatgtaaga tacacatagg gataaaatgt
     1921 gataactaat cagcggtacc gggcccggaa cagcaatagg gttgctacca gtatctataa
     1981 taccctcgtt ggtctcccta acatgtaggt ggcggagggg agatatacaa tagaacagat
     2041 accagacaag acataatggg ctaaacaaga ctacaccaat tacactgcct cattgatggt
     2101 ggtacataac gaactaatac tgtagcccta gacttgatag ccatcatcat atcgaagttt
     2161 cactaccctt tttccatttg ccatctattg aagtaataat aggcgcatgc aacttctttt
     2221 cttttttttt cttttctctc tcccccgttg ttgtctcacc atatccgcaa tgacaaaaaa
     2281 atgatggaag acactaaagg aaaaaattaa cgacaaagac agcaccaaca gatgtcgttg
     2341 ttccagagct gatgaggggt atctcgaagc acacgaaact ttttccttcc ttcattcacg
     2401 cacactactc cctaatgagc aacggtatac ggccttcctt ccagttactt gaatttgaaa
     2461 taaaaaaaag tttgctgtct tgctatcaag tataaataga cctgcaatta ttaatctttt
     2521 gtttcctcgt cattgttctc gttccctttc ttccttgttt ctttttctgc acaatatttc
     2581 aagctatacc aagcatacaa tcaactatct cactcgactc taaaatgacg gttgaccatg
     2641 atttcaatag cgaagatatt ttattcccca tagaaagcat gagtagtata caatacgtgg
     2701 agaataataa cccaaataat attaacaacg atgttatccc gtattctcta gatatcaaaa
     2761 acactgtctt agatagtgcg gatctcaatg acattcaaaa tcaagaaact tcactgaatt
     2821 tggggcttcc tccactatct ttcgactctc cactgcccgt aacggaaacg ataccatcca
     2881 ctaccgataa cagcttgcat ttgaaagctg atagcaacaa aaatcgcgat gcaagaacta
     2941 ttgaaaatga tagtgaaatt aagagtacta ataatgctag tggctctggg gcaaatcaat
     3001 acacaactct tacttcacct tatcctatga acgacatttt gtacaacatg aacaatccgt
     3061 tacaatcacc gtcaccttca tcggtacctc aaaatccgac tataaatcct cccataaata
     3121 cagcaagtaa cgaaactaat ttatcgcctc aaacttcaaa tggtaatgaa actcttatat
     3181 ctcctcgagc ccaacaacat acgtccatta aagataatcg tctgtcctta cctaatggtg
     3241 ctaattcgaa tcttttcatt gacactaacc caaacaattt gaacgaaaaa ctaagaaatc
     3301 aattgaactc agatacaaat tcatattcta actccatttc taattcaaac tccaattcta
     3361 cgggtaattt aaattccagt tattttaatt cactgaacat agactccatg ctagatgatt
     3421 acgtttctag tgatctctta ttgaatgatg atgatgatga cactaattta tcacgccgaa
     3481 gatttagcga cgttataaca aaccaatttc cgtcaatgac aaattcgagg aattctattt
     3541 ctcactcttt ggacctttgg aaccatccga aaattaatcc aagcaataga aatacaaatc
     3601 tcaatatcac tactaattct acctcaagtt ccaatgcaag tccgaatacc actactatga
     3661 acgcaaatgc agactcaaat attgctggca acccgaaaaa caatgacgct accatagaca
     3721 atgagttgac acagattctt aacgaatata atatgaactt caacgataat ttgggcacat
     3781 ccgcttctgg caagaacaaa tctgcttgcc caagttcttt tgatgccaat gctatgacaa
     3841 agataaatcc aagtcagcaa ttacagcaac agctaaaccg agttcaacac aagcagctca
     3901 cctcgtcaca taataacagt agcactaaca tgaaatcctt caacagcgat ctttattcaa
     3961 gaaggcaaag agcttcttta cccataatcg atgattcact aagctacgac ctggttaata
     4021 agcaggatga agaccccaag aacgatatgc tgccgaattc aaatttgagt tcatctcaac
     4081 aatttatcaa accgtctatg attctttcag acaatgcgtc cgttattgcg aaagtggcga
     4141 ctacaggctt gagtaatgat atgccatttt tgacagagga aggtgaacaa aatgctaatt
     4201 ctactccaaa tttcgatctt tccatcactc aaatgaatat ggctccatta tcgcctgcat
     4261 catcatcctc cacgtctctt gcaacaaatc atttctatca ccatttccca cagcagggtc
     4321 accataccat gaactctaaa atcggttctt cccttcggag gcggaagtct gctgtgcctt
     4381 tgataggtac ggtgccgctt acaaatcaac aaaataatat aagcagtagt agtgtcaact
     4441 caactggcaa tggtgctggg gttacgaagg aaagaaggcc aagttacagg agaaaatcaa
     4501 tgacaccgtc cagaagatca agtgtcgtaa tagaatcaac aaaggaactc gaggagaaac
     4561 cgttccactg tcacatttgt cccaagagct ttaagcgcag cgaacatttg aaaaggcatg
     4621 tgagatctgt tcactctaac gaacgaccat ttgcttgtca catatgcgat aagaaattta
     4681 gtagaagcga taatttgtcg caacacatca agactcataa aaaacatgga gacattggat
     4741 cctcaggatc tggttccggc agcaagggcg aggaggataa catggccatc atcaaggagt
     4801 tcatgcgctt caaggtgcac atggagggct ccgtgaacgg ccacgagttc gagatcgagg
     4861 gcgagggcga gggccgcccc tacgagggca cccagaccgc caagctgaag gtgaccaagg
     4921 gtggccccct gcccttcgcc tgggacatcc tgtcccctca gttcatgtac ggctccaagg
     4981 cctacgtgaa gcaccccgcc gacatccccg actacttgaa gctgtccttc cccgagggct
     5041 tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt ggcgaccgtg acccaggact
     5101 cctccctgca ggacggcgag ttcatctaca aggtgaagct gcgcggcacc aacttcccct
     5161 ccgacggccc cgtaatgcag aagaagacca tgggctggga ggcctcctcc gagcggatgt
     5221 accccgagga cggcgccctg aagggcgaga tcaagcagag gctgaagctg aaggacggcg
     5281 gccactacga cgctgaggtc aagaccacct acaaggccaa gaagcccgtg cagctgcccg
     5341 gcgcctacaa cgtcaacatc aagttggaca tcacctccca caacgaggac tacaccatcg
     5401 tggaacagta cgaacgcgcc gagggccgcc actccaccgg cggcatggac gagctgtaca
     5461 aggcggccgc caccgcggtg gagctctaag caaatagcta aattatatac gaattaatat
     5521 tatgattaag tgtttacgtg agtgcgatat ttttattact atcttataca gttgtatata
     5581 ctctataaaa tgagttgtct attaattaac gcgatgaatg ctttctgggt ttacctctcc
     5641 aacaactcta gtttacttct caatacattc aattgtattt gatttgtcaa tacttcatca
     5701 ttaatcaatt ctatagtttt gtttttctcg tttatttcca aatttaatgc atcaatttta
     5761 ttattcaatt tgtcgttgat tttggttaat gattttatgg tttgatctct ggcattgatt
     5821 gtttgtgtta gtttttcatt attgataatt aaattattta agttagttat caactcggtg
     5881 ttttcaagtt tcaagttttc aatttcttta gagtttatta gatttgtcaa agtttctgaa
     5941 ttgcttgatt ggtcctgtag aagagtattt gttgttgtgg ataattgatt caatttttga
     6001 gacaattgct ggaaggcgtt gaaatatcta gcatcaatct catggttttt ttcccgagag
     6061 tctcgtagat tcaattgttt taatatatct tgggaccact cttgatttga actcatggaa
     6121 attaaactgg gtgttgtgtt gtggtgtaat gattgtaccc cctttgctta taattgtgtg
     6181 gttccgatgc tgacttactg ggtattatat gtgtgcccaa tagaaagaga acaattgacc
     6241 cggttattgc aaggaaaatt tcaagtcttg taaaagcata taaaaatagt tcaggcactc
     6301 cgaaatactt ggttggcgtg tttcgtaatc aacctaagga ggatgttttg gctctggtca
     6361 atgattacgg cattgatatc gtccaactgc atggagatga gtcgtggcga gaataccaag
     6421 agttcctcgg tttgccagtt attaaaagac tcgtatttcc aaaagactgc aacatactac
     6481 tcagtgcagc ttcacagaaa cctcattcgt ttattccctt gtttgattca gaaggccggc
     6541 catttaaatg tttaaacggc cacgctggcc ccctatagtg agtcgtatta caattcactg
     6601 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt
     6661 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
     6721 tcccaacagt tgcgcagcct gaatggcgaa tggacgcgcc ctgtagcggc gcattaagcg
     6781 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
     6841 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
     6901 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
     6961 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
     7021 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
     7081 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt
     7141 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc
     7201 ttacaatttc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat
     7261 agggtaataa ctgatataat taaattgaag ctctaatttg tgagtttagt attaggatct
     7321 ctacccttgg cgaaaagtcc tctgccaaca atgatgatat ctgatccacc acttacaact
     7381 tcgtcgacgg ttctgtactg ctgacccaat atgcgtatat ataccaatct aagtctgtgc
     7441 tccttccttc gttcttcctt ctgttcggag attaccgaat caaaaaaatt tcaaggaaac
     7501 cgaaatcaaa aaaaagaata aaaaaaaaat gatgaattga aaaggtggta tggtgcactc
     7561 tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg
     7621 ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg
     7681 tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa
     7741 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga
     7801 cgtcggcgcg ccgaggacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
     7861 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     7921 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     7981 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     8041 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
     8101 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
     8161 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
     8221 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
     8281 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     8341 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
     8401 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     8461 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
     8521 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     8581 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     8641 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
     8701 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     8761 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     8821 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     8881 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     8941 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     9001 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     9061 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     9121 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     9181 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     9241 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     9301 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     9361 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     9421 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     9481 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     9541 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     9601 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     9661 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     9721 gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc
     9781 cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg
     9841 cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca
     9901 ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg
     9961 aaacagctat gaccatgatt acgccaagct cggaattaac cctcactaaa gggaacaaaa
    10021 gctg
//
