LOCUS       sequence-202560-        9709 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Yeast Expression Plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9709)
  AUTHORS   Stewart-Ornstein J, Chen S, Bhatnagar R, Weissman JS, El-Samad H
  TITLE     Model-guided optogenetic study of PKA signaling in budding yeast.
  JOURNAL   Mol Biol Cell. 2017 Jan 1;28(1):221-227. doi: 
            10.1091/mbc.E16-06-0354. Epub 2016 Nov 9.
  PUBMED    28035051
REFERENCE   2  (bases 1 to 9709)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1091/mbc.E16-06-0354"; journalName: "Mol Biol Cell"; date: 
            "2017-01-1- 1"; volume: "28"; issue: "1"; pages: "221-227"
COMMENT     Sequence Label: pHES553  [prGal1-RAS2(KAN) pnh604]
FEATURES             Location/Qualifiers
     source          1..9709
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        2128..2569
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     protein_bind    2128..2245
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     2540..2563
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     gene            3629..4985
                     /label=kanMX
                     /note="yeast selectable marker conferring kanamycin 
                     resistance (Wach et al., 1994)"
     promoter        3629..3972
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             3973..4782
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(4040..4059)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      4788..4985
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     primer_bind     complement(6256..6275)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6257..6275)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6282..6299)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6282..6298)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6291..6313)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6439..6894
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6526..6545)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6736..6757
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7250..7269)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7369..7391
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7429..7447)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7532..7636
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7637..7705,7706..8497)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7855..7874)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8668..9256
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9157..9176
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9410..9427
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9544..9565
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9580..9585,9586..9603,9604..9610)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9618..9634
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9623..9645
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9642..9658
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9642..9658
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        9679..9697
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ggccggccat ttaaatgttt aaacggccac gctggccata tttctaccaa tctctcaaca
       61 ctgagtaatg gtagttataa gaaagagacc gagttaggga cagttagagg cggtggagat
      121 attccttatg gcatgtctgg cgatgataaa acttttcaaa cggcagcccc gatctaaaag
      181 agctgacagg gaaatggtca gaaaaagaaa cgtgcacccg cccgtctgga cgcgccgctc
      241 acccgcacgg cagagaccaa tcagtaaaaa tcaacggtta acgacattac tatatatata
      301 atataggaag catttaatag aacagcatcg taatatatgt gtactttgca gttatgacgc
      361 cagatggcag tagtggaaga tattctttat tgaaaaatag cttgtcacct tacgtacaat
      421 cttgatccgg agcccgtcgc tttgagagaa atcagaagat tccaaaagtc tactgaactt
      481 ttgatcagaa agttgccttt ccaaagacta gtcagagaaa tcgcccaaga tttcaagacc
      541 gatctaagat tccaatcctc tgccatcggt gctctacagg aatccgttga agcttacttg
      601 gtctctttgt tcgaagacac caacttggcc gctatccacg ctaagcgtgt taccatccaa
      661 aagaaggata tcaagttggc tagaagattg agaggtgaaa gatcctaagc acacagggat
      721 tgttgtacaa ttacttttta tgattttaga tacacacaaa tcttttctac gattaccttt
      781 catttatttt cgtttcatgt tttattaatc attctttttg tatatttcac atcgggtggg
      841 gtttttatat taaattttaa taacataatc ttttaaagtt tctactttct atcctgtata
      901 ttatttccat atctatatct atattttcaa gcttgttcac atccttatcg gaattgttcc
      961 agcgtttttg tacagaaaat gatgttatgg taacgagcta tatcagacct actagtctta
     1021 tagagctgta cagagtcttg gaaacataac caacacacca acaagatgtc atttgattcg
     1081 ttactcgaca agaatgataa gctggtaaaa gtttgcggga ttcaaaccgt cgaggctgcc
     1141 gaaactgcgc ttcaagcagg cgctgatttg atagggatca tatgtgtccc caacaggaag
     1201 cggactatcg agagcgctgt ggctcgtgaa atatccaaat tgattcacaa atcaggtact
     1261 acaaagctgg tgggggtgtt caggaatcaa tctgttgagg acgtacatcg gctttctgag
     1321 gaatatgacc ttgacataat ccaattacat ggtgatgaat catggccaga gtactataac
     1381 gtcattaaga aaccaataat caaaagagtc atattcccta gagatgtcga tgttgtaaca
     1441 caagtgtgtc aaagaaaacc cttggtatgt ctaccattgt tcgactccga ggcaggcggt
     1501 acaggtgaaa agctggactg gtccagtatt tcctcctggg caagcgaaca gaataacgta
     1561 ttttatatac tagcgggtgg actaactgca gaaaacgtca tggaggcggt ggggttgcct
     1621 ggtgtaattg gtgttgacgt ctcaggtggt gtagagactg acggcgtcaa agacaatgac
     1681 aagataatta aatatgtaca aaatgcaaag aaacaatgaa ttacgaagag aagtctcaat
     1741 gaggttttac aaacaatcct ataatacata tatgccactt agatatactt tgaatactga
     1801 atatactgac tcattatatt ctttaattgt agtttctcaa aaatattcat acaggttagc
     1861 tatgaattca tgctataatt tccattttgt tcatgtaaga tacacatagg gataaaatgt
     1921 gataactaat cagcggtacc gggcccttat attgaatttt caaaaattct tacttttttt
     1981 tggatggacg caaagaagtt taatatccat atctaatctt acttatatgt tgtggaaatg
     2041 taaagagccc cattatctta gcctaaaaaa accttctctt tggaactttc agtaatacgc
     2101 ttaactgctc attgctatat tgaagtacgg attagaagcc gccgagcggg cgacagccct
     2161 ccgacggaag actctcctcc gtgcgtcctc gtcttcaccg gtcgcgttcc tgaaacgcag
     2221 atgtgcctcg cgccgcactg ctccgaacaa taaagattct acaatactag cttttatggt
     2281 tatgaagagg aaaaattggc agtaacctgg ccccacaaac cttcaaatta acgaatcaaa
     2341 ttaacaacca taggatgata atgcgattag ttttttagcc ttgtttctgg ggtaattaat
     2401 cagcgaagcg atgatttttg atctattaac agatatataa atggaaaagc tgcataacca
     2461 ctttaactaa tactttcaac attttcagtt tgtattactt cttattcaaa tgtcataaaa
     2521 gtatcaacaa aaaattgtta atatacctct atactttaac gtcaaggaga aaaaactata
     2581 ctcgagaata aaatgccttt gaacaagtcg aacataagag agtacaagct agtcgtcgtt
     2641 ggtggtggtg gtgttggtaa aaatgctttg accatacaat tgacccaatc gcactttgta
     2701 gatgaatacg atcccacaat tgaggattca tacagggagc aagtggtgat tgatgatgaa
     2761 gtgtctatat tggacatttt ggatactgca gggcaggaag aatactctgc tatgagggaa
     2821 caatacatgc gcaacggcga aggattccta ttggtttact ctataacgtc caagtcgtct
     2881 cttgatgagc ttatgactta ctatcaacag atattgagag tcaaagatac cgactatgtt
     2941 ccaattgtgg ttgttggtaa caaatctgat ttagaaaacg aaaaacaggt ctcttaccag
     3001 gacgggttga acatggcaaa gcaaatgaac gctcctttct tggagacatc tgctaagcaa
     3061 gcaatcaacg tggaagaggc gttttacact ctagcacgtt tagttagaga cgaaggcggc
     3121 aagtacaaca agactttgac ggaaaatgac aactccaagc aaacttctca agatacaaaa
     3181 gggagcggtg ccaactctgt gcctagaaat agcggtggcc acaggaagat gagcaatgct
     3241 gccaacggta aaaatgtgaa cagtagcaca actgtcgtga atgccaggaa tgcaagcata
     3301 gagagtaaga cagggttggc aggcaaccag gcgacaaatg gtaagacaca aactgatcgc
     3361 accaatatag acaattccac gggccaagct ggtcaggcca acgctcaaag cgctaatacg
     3421 gttaataatc gtgtaaataa taatagtaag gccggtcaag tttcaaatgc taaacaggct
     3481 aggaagcagc aagctgcacc cggcggtaac accagtgaag cctccaagag cggatcgggt
     3541 ggctgttgta ttataagtta aggatcggat aacagggtaa tatagatctg tttagcttgc
     3601 ctcgtccccg ccgggtcacc cggccagcga catggaggcc cagaataccc tccttgacag
     3661 tcttgacgtg cgcagctcag gggcatgatg tgactgtcgc ccgtacattt agcccataca
     3721 tccccatgta taatcatttg catccataca ttttgatggc cgcacggcgc gaagcaaaaa
     3781 ttacggctcc tcgctgcaga cctgcgagca gggaaacgct cccctcacag acgcgttgaa
     3841 ttgtccccac gccgcgcccc tgtagagaaa tataaaaggt taggatttgc cactgaggtt
     3901 cttctttcat atacttcctt ttaaaatctt gctaggatac agttctcaca tcacatccga
     3961 acataaacaa ccatgggtaa ggaaaagact cacgtttcga ggccgcgatt aaattccaac
     4021 atggatgctg atttatatgg gtataaatgg gctcgcgata atgtcgggca atcaggtgcg
     4081 acaatctatc gattgtatgg gaagcccgat gcgccagagt tgtttctgaa acatggcaaa
     4141 ggtagcgttg ccaatgatgt tacagatgag atggtcagac taaactggct gacggaattt
     4201 atgcctcttc cgaccatcaa gcattttatc cgtactcctg atgatgcatg gttactcacc
     4261 actgcgatcc ccggcaaaac agcattccag gtattagaag aatatcctga ttcaggtgaa
     4321 aatattgttg atgcgctggc agtgttcctg cgccggttgc attcgattcc tgtttgtaat
     4381 tgtcctttta acagcgatcg cgtatttcgt ctcgctcagg cgcaatcacg aatgaataac
     4441 ggtttggttg atgcgagtga ttttgatgac gagcgtaatg gctggcctgt tgaacaagtc
     4501 tggaaagaaa tgcataagct tttgccattc tcaccggatt cagtcgtcac tcatggtgat
     4561 ttctcacttg ataaccttat ttttgacgag gggaaattaa taggttgtat tgatgttgga
     4621 cgagtcggaa tcgcagaccg ataccaggat cttgccatcc tatggaactg cctcggtgag
     4681 ttttctcctt cattacagaa acggcttttt caaaaatatg gtattgataa tcctgatatg
     4741 aataaattgc agtttcattt gatgctcgat gagtttttct aatcagtact gacaataaaa
     4801 agattcttgt tttcaagaac ttgtcatttg tatagttttt ttatattgta gttgttctat
     4861 tttaatcaaa tgttagcgtg atttatattt tttttcgcct cgacatcatc tgcccagatg
     4921 cgaagttaag tgcgcagaaa gtaatatcat gcgtcaatcg tatgtgaatg ctggtcgcta
     4981 tactgctgtc gattcgatac taacgccgcc atccagttta aacgagctcg aattcatcga
     5041 tgatatcaga tccactagtg gcctatgcgg ccgccaccgc ggtggagctc taagcaaata
     5101 gctaaattat atacgaatta atattatgat taagtgttta cgtgagtgcg atatttttat
     5161 tactatagct ctaagcaaat agctaaatta tatacgaatt aatattatga ttaagtgttt
     5221 acgtgagtgc gatattttta ttactatctt atacagttgt atatactcta taaaatgagt
     5281 tgtctattaa ttaacgcgat gaatgctttc tgggtttacc tctccaacaa ctctagttta
     5341 cttctcaata cattcaattg tatttgattt gtcaatactt catcattaat caattctata
     5401 gttttgtttt tctcgtttat ttccaaattt aatgcatcaa ttttattatt caatttgtcg
     5461 ttgattttgg ttaatgattt tatggtttga tctctggcat tgattgtttg tgttagtttt
     5521 tcattattga taattaaatt atttaagtta gttatcaact cggtgttttc aagtttcaag
     5581 ttttcaattt ctttagagtt tattagattt gtcaaagttt ctgaattgct tgattggtcc
     5641 tgtagaagag tatttgttgt tgtggataat tgattcaatt tttgagacaa ttgctggaag
     5701 gcgttgaaat atctagcatc aatctcatgg tttttttccc gagagtctcg tagattcaat
     5761 tgttttaata tatcttggga ccactcttga tttgaactca tggaaattaa actgggtgtt
     5821 gtgttgtggt gtaatgattg tacccccttt gcttataatt gtgtggttcc gatgctgact
     5881 tactgggtat tatatgtgtg cccaatagaa agagaacaat tgacccggtt attgcaagga
     5941 aaatttcaag tcttgtaaaa gcatataaaa atagttcagg cactccgaaa tacttggttg
     6001 gcgtgtttcg taatcaacct aaggaggatg ttttggctct ggtcaatgat tacggcattg
     6061 atatcgtcca actgcatgga gatgagtcgt ggcgagaata ccaagagttc ctcggtttgc
     6121 cagttattaa aagactcgta tttccaaaag actgcaacat actactcagt gcagcttcac
     6181 agaaacctca ttcgtttatt cccttgtttg attcagaagg ccggccattt aaatgtttaa
     6241 acggccacgc tggcccccta tagtgagtcg tattacaatt cactggccgt cgttttacaa
     6301 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct
     6361 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
     6421 agcctgaatg gcgaatggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
     6481 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
     6541 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
     6601 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg
     6661 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
     6721 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg
     6781 tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc
     6841 tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttcctgat
     6901 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatagggt aataactgat
     6961 ataattaaat tgaagctcta atttgtgagt ttagtattag gatctctacc cttggcgaaa
     7021 agtcctctgc caacaatgat gatatctgat ccaccactta caacttcgtc gacggttctg
     7081 tactgctgac ccaatatgcg tatatatacc aatctaagtc tgtgctcctt ccttcgttct
     7141 tccttctgtt cggagattac cgaatcaaaa aaatttcaag gaaaccgaaa tcaaaaaaaa
     7201 gaataaaaaa aaaatgatga attgaaaagg tggtatggtg cactctcagt acaatctgct
     7261 ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac
     7321 gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca
     7381 tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac
     7441 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtcg gcgcgccgag
     7501 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     7561 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     7621 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     7681 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     7741 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     7801 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     7861 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     7921 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     7981 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     8041 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     8101 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     8161 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
     8221 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     8281 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     8341 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     8401 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     8461 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     8521 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     8581 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     8641 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     8701 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     8761 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     8821 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     8881 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     8941 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     9001 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     9061 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     9121 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     9181 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     9241 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     9301 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     9361 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     9421 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
     9481 tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc
     9541 aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc
     9601 tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca
     9661 tgattacgcc aagctcggaa ttaaccctca ctaaagggaa caaaagctg
//
