LOCUS       sequence-20257-e         688 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 688)
  AUTHORS   Ma JB, Yuan YR, Meister G, Pei Y, Tuschl T, Patel DJ
  TITLE     Structural basis for 5'-end-specific recognition of guide RNA by the
            A. fulgidus Piwi protein.
  JOURNAL   Nature. 2005 Mar 31. 434(7033):666-70.
  PUBMED    15800629
REFERENCE   2  (bases 1 to 688)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2005 Mar 31. 434(7033):666-70."
COMMENT     Sequence Label: pDESTFLAGHApuro3-Ago2K533A
FEATURES             Location/Qualifiers
     source          1..688
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      28..37
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             37..60
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             61..87
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    103..127
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gatagtggtg gtggaattct gatcgccgcc gccatggact acaaggacga cgatgacaag
       61 tacccttatg acgtgcccga ttacgctttg aaccagatat caacaagttt gtacaaaaaa
      121 gcaggctcca ccatgggtgt tctctctgcc attcccgcac ttgcacctcc tgcgccgccg
      181 ccccccatcc aaggatatgc cttcaagcct ccacctagac ccgactttgg gacctccggg
      241 agaacaatca aattacaggc caatttcttc gaaatggaca tccccaaaat tgacatctat
      301 cattatgaat tggatatcaa gccagagaag tgcccgagga gagttaacag ggaaatcgtg
      361 gaacacatgg tccagcactt taaaacacag atctttgggg atcggaagcc cgtgtttgac
      421 ggcaggaaga atctatacac agccatgccc cttccgattg ggagggacaa ggtggagctg
      481 gaggtcacgc tgccaggaga aggcaaggat cgcatcttca aggtgtccat caagtgggtg
      541 tcctgcgtga gcttgcaggc gttacacgat gcactttcag ggcggctgcc cagcgtccct
      601 tttgagacga tccaggccct ggacgtggtc atgaggcact tgccatccat gaggtacacc
      661 cccgtgggcc gctccttctt caccgcgt
//
