LOCUS       sequence-20258-e         688 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 688)
  AUTHORS   Ma JB, Yuan YR, Meister G, Pei Y, Tuschl T, Patel DJ
  TITLE     Structural basis for 5'-end-specific recognition of guide RNA by the
            A. fulgidus Piwi protein.
  JOURNAL   Nature. 2005 Mar 31. 434(7033):666-70.
  PUBMED    15800629
REFERENCE   2  (bases 1 to 688)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2005 Mar 31. 434(7033):666-70."
COMMENT     Sequence Label: pDESTFLAGHApuro3-Ago2Q545A
FEATURES             Location/Qualifiers
     source          1..688
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      27..36
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             36..59
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             60..86
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    102..126
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 atagtggtgg tggaattctg atcgccgccg ccatggacta caaggacgac gatgacaagt
       61 acccttatga cgtgcccgat tacgctttga accagatatc aacaagtttg tacaaaaaag
      121 caggctccac catgggtgtt ctctctgcca ttcccgcact tgcacctcct gcgccgccgc
      181 cccccatcca aggatatgcc ttcaagcctc cacctagacc cgactttggg acctccggga
      241 gaacaatcaa attacaggcc aatttcttcg aaatggacat ccccaaaatt gacatctatc
      301 attatgaatt ggatatcaag ccagagaagt gcccgaggag agttaacagg gaaatcgtgg
      361 aacacatggt ccagcacttt aaaacacaga tctttgggga tcggaagccc gtgtttgacg
      421 gcaggaagaa tctatacaca gccatgcccc ttccgattgg gagggacaag gtggagctgg
      481 aggtcacgct gccaggagaa ggcaaggatc gcatcttcaa ggtgtccatc aagtgggtgt
      541 cctgcgtgag cttgcaggcg ttacacgatg cactttcagg gcggctgccc agcgtccctt
      601 ttgagacgat ccaggccctg gacgtggtca tgaggcactt gccatccatg aggtacaccc
      661 ccgtgggccg ctccttcttc accgcgtc
//
