LOCUS       sequence-202585-        3602 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression plasmid for Env from the DU422.1 HIV-1 isolate.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3602)
  AUTHORS   Heredia JD, Park J, Choi H, Gill KS, Procko E
  TITLE     Conformational engineering of HIV-1 Env based on mutational 
            tolerance in the CD4 and PG16 bound states.
  JOURNAL   J Virol. 2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19.
  PUBMED    30894475
REFERENCE   2  (bases 1 to 3602)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19."
COMMENT     Sequence Label: pCEP4-DU422gp160
FEATURES             Location/Qualifiers
     source          1..3602
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     rep_origin      392..2181
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
ORIGIN
        1 cggggcagtg catgtaatcc cttcagttgg ttggtacaac ttgccaactg ggccctgttc
       61 cacatgtgac acgggggggg accaaacaca aaggggttct ctgactgtag ttgacatcct
      121 tataaatgga tgtgcacatt tgccaacact gagtggcttt catcctggag cagactttgc
      181 agtctgtgga ctgcaacaca acattgcctt tatgtgtaac tcttggctga agctcttaca
      241 ccaatgctgg gggacatgta cctcccaggg gcccaggaag actacgggag gctacaccaa
      301 cgtcaatcag aggggcctgt gtagctaccg ataagcggac cctcaagagg gcattagcaa
      361 tagtgtttat aaggccccct tgttaaccct aaacgggtag catatgcttc ccgggtagta
      421 gtatatacta tccagactaa ccctaattca atagcatatg ttacccaacg ggaagcatat
      481 gctatcgaat tagggttagt aaaagggtcc taaggaacag cgatatctcc caccccatga
      541 gctgtcacgg ttttatttac atggggtcag gattccacga gggtagtgaa ccattttagt
      601 cacaagggca gtggctgaag atcaaggagc gggcagtgaa ctctcctgaa tcttcgcctg
      661 cttcttcatt ctccttcgtt tagctaatag aataactgct gagttgtgaa cagtaaggtg
      721 tatgtgaggt gctcgaaaac aaggtttcag gtgacgcccc cagaataaaa tttggacggg
      781 gggttcagtg gtggcattgt gctatgacac caatataacc ctcacaaacc ccttgggcaa
      841 taaatactag tgtaggaatg aaacattctg aatatcttta acaatagaaa tccatggggt
      901 ggggacaagc cgtaaagact ggatgtccat ctcacacgaa tttatggcta tgggcaacac
      961 ataatcctag tgcaatatga tactggggtt attaagatgt gtcccaggca gggaccaaga
     1021 caggtgaacc atgttgttac actctatttg taacaagggg aaagagagtg gacgccgaca
     1081 gcagcggact ccactggttg tctctaacac ccccgaaaat taaacggggc tccacgccaa
     1141 tggggcccat aaacaaagac aagtggccac tctttttttt gaaattgtgg agtgggggca
     1201 cgcgtcagcc cccacacgcc gccctgcggt tttggactgt aaaataaggg tgtaataact
     1261 tggctgattg taaccccgct aaccactgcg gtcaaaccac ttgcccacaa aaccactaat
     1321 ggcaccccgg ggaatacctg cataagtagg tgggcgggcc aagatagggg cgcgattgct
     1381 gcgatctgga ggacaaatta cacacacttg cgcctgagcg ccaagcacag ggttgttggt
     1441 cctcatattc acgaggtcgc tgagagcacg gtgggctaat gttgccatgg gtagcatata
     1501 ctacccaaat atctggatag catatgctat cctaatctat atctgggtag cataggctat
     1561 cctaatctat atctgggtag catatgctat cctaatctat atctgggtag tatatgctat
     1621 cctaatttat atctgggtag cataggctat cctaatctat atctgggtag catatgctat
     1681 cctaatctat atctgggtag tatatgctat cctaatctgt atccgggtag catatgctat
     1741 cctaatagag attagggtag tatatgctat cctaatttat atctgggtag catatactac
     1801 ccaaatatct ggatagcata tgctatccta atctatatct gggtagcata tgctatccta
     1861 atctatatct gggtagcata ggctatccta atctatatct gggtagcata tgctatccta
     1921 atctatatct gggtagtata tgctatccta atttatatct gggtagcata ggctatccta
     1981 atctatatct gggtagcata tgctatccta atctatatct gggtagtata tgctatccta
     2041 atctgtatcc gggtagcata tgctatcctc atgcatatac agtcagcata tgatacccag
     2101 tagtagagtg ggagtgctat cctttgcata tgccgccacc tcccaagggg gcgtgaattt
     2161 tcgctgcttg tccttttcct gctggttgct cccattctta ggtgaattta aggaggccag
     2221 gctaaagccg tcgcatgtct gattgctcac caggtaaatg tcgctaatgt tttccaacgc
     2281 gagaaggtgt tgagcgcgga gctgagtgac gtgacaacat gggtatgccc aattgcccca
     2341 tgttgggagg acgaaaatgg tgacaagaca gatggccaga aatacaccaa cagcacgcat
     2401 gatgtctact ggggatttat tctttagtgc gggggaatac acggctttta atacgattga
     2461 gggcgtctcc taacaagtta catcactcct gcccttcctc accctcatct ccatcacctc
     2521 cttcatctcc gtcatctccg tcatcaccct ccgcggcagc cccttccacc ataggtggaa
     2581 accagggagg caaatctact ccatcgtcaa agctgcacac agtcaccctg atattgcagg
     2641 taggagcggg ctttgtcata acaaggtcct taatcgcatc cttcaaaacc tcagcaaata
     2701 tatgagtttg taaaaagacc atgaaataac agacaatgga ctcccttagc gggccaggtt
     2761 gtgggccggg tccaggggcc attccaaagg ggagacgact caatggtgta agacgacatt
     2821 gtggaatagc aagggcagtt cctcgcctta ggttgtaaag ggaggtctta ctacctccat
     2881 atacgaacac accggcgacc caagttcctt cgtcggtagt cctttctacg tgactcctag
     2941 ccaggagagc tcttaaacct tctgcaatgt tctcaaattt cgggttggaa cctccttgac
     3001 cacgatgctt tccaaaccac cctccttttt tgcgcctgcc tccatcaccc tgaccccggg
     3061 gtccagtgct tgggccttct cctgggtcat ctgcggggcc ctgctctatc gctcccgggg
     3121 gcacgtcagg ctcaccatct gggccacctt cttggtggta ttcaaaataa tcggcttccc
     3181 ctacagggtg gaaaaatggc cttctacctg gagggggcct gcgcggtgga gacccggatg
     3241 atgatgactg actactggga ctcctgggcc tcttttctcc acgtccacga cctctccccc
     3301 tggctctttc acgacttccc cccctggctc tttcacgtcc tctaccccgg cggcctccac
     3361 tacctcctcg accccggcct ccactacctc ctcgaccccg gcctccactg cctcctcgac
     3421 cccggcctcc acctcctgct cctgcccctc ctgctcctgc ccctcctcct gctcctgccc
     3481 ctcctgcccc tcctgctcct gcccctcctg cccctcctgc tcctgcccct cctgcccctc
     3541 ctgctcctgc ccctcctgcc cctcctcctg ctcctgcccc tcctgcccct cctcctgctc
     3601 ct
//
