LOCUS       sequence-202586-        5679 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression plasmid for Env from the DU422.1 HIV-1 isolate.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5679)
  AUTHORS   Heredia JD, Park J, Choi H, Gill KS, Procko E
  TITLE     Conformational engineering of HIV-1 Env based on mutational 
            tolerance in the CD4 and PG16 bound states.
  JOURNAL   J Virol. 2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19.
  PUBMED    30894475
REFERENCE   2  (bases 1 to 5679)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19."
COMMENT     Sequence Label: pCEP4-DU422gp160
FEATURES             Location/Qualifiers
     source          1..5679
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        86..231
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             274..1311
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(1309..1328)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    1353..1401
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        2055..2434
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2435..2638
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2589..2609
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2635..2659
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     polyA_signal    5271..5405
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5295..5314)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5349..5368
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(5548..5570)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 gccctgcttc atccccgtgg cccgttgctc gcgtttgctg gcggtgtccc cggaagaaat
       61 atatttgcat gtctttagtt ctatgatgac acaaaccccg cccagcgtct tgtcattggc
      121 gaattcgaac acgcagatgc agtcggggcg gcgcggtccg aggtccactt cgcatattaa
      181 ggtgacgcgt gtggcctcga acaccgagcg accctgcagc gacccgctta acagcgtcaa
      241 cagcgtgccg cagatcccgg ggggcaatga gatatgaaaa agcctgaact caccgcgacg
      301 tctgtcgaga agtttctgat cgaaaagttc gacagcgtct ccgacctgat gcagctctcg
      361 gagggcgaag aatctcgtgc tttcagcttc gatgtaggag ggcgtggata tgtcctgcgg
      421 gtaaatagct gcgccgatgg tttctacaaa gatcgttatg tttatcggca ctttgcatcg
      481 gccgcgctcc cgattccgga agtgcttgac attggggaat tcagcgagag cctgacctat
      541 tgcatctccc gccgtgcaca gggtgtcacg ttgcaagacc tgcctgaaac cgaactgccc
      601 gctgttctgc agccggtcgc ggaggccatg gatgcgatcg ctgcggccga tcttagccag
      661 acgagcgggt tcggcccatt cggaccgcaa ggaatcggtc aatacactac atggcgtgat
      721 ttcatatgcg cgattgctga tccccatgtg tatcactggc aaactgtgat ggacgacacc
      781 gtcagtgcgt ccgtcgcgca ggctctcgat gagctgatgc tttgggccga ggactgcccc
      841 gaagtccggc acctcgtgca cgcggatttc ggctccaaca atgtcctgac ggacaatggc
      901 cgcataacag cggtcattga ctggagcgag gcgatgttcg gggattccca atacgaggtc
      961 gccaacatct tcttctggag gccgtggttg gcttgtatgg agcagcagac gcgctacttc
     1021 gagcggaggc atccggagct tgcaggatcg ccgcggctcc gggcgtatat gctccgcatt
     1081 ggtcttgacc aactctatca gagcttggtt gacggcaatt tcgatgatgc agcttgggcg
     1141 cagggtcgat gcgacgcaat cgtccgatcc ggagccggga ctgtcgggcg tacacaaatc
     1201 gcccgcagaa gcgcggccgt ctggaccgat ggctgtgtag aagtactcgc cgatagtgga
     1261 aaccgacgcc ccagcactcg tccggatcgg gagatggggg aggctaactg aaacacggaa
     1321 ggagacaata ccggaaggaa cccgcgctat gacggcaata aaaagacaga ataaaacgca
     1381 cgggtgttgg gtcgtttgtt cataaacgcg gggttcggtc ccagggctgg cactctgtcg
     1441 ataccccacc gagaccccat tggggccaat acgcccgcgt ttcttccttt tccccacccc
     1501 accccccaag ttcgggtgaa ggcccagggc tcgcagccaa cgtcggggcg gcaggccctg
     1561 ccatagccac tggccccgtg ggttagggac ggggtccccc atggggaatg gtttatggtt
     1621 cgtgggggtt attattttgg gcgttgcgtg gggtcaggtc cacgactgga ctgagcagac
     1681 agacccatgg tttttggatg gcctgggcat ggaccgcatg tactggcgcg acacgaacac
     1741 cgggcgtctg tggctgccaa acacccccga cccccaaaaa ccaccgcgcg gatttctggc
     1801 gtgccaagct agtcgaccaa ttctcatgtt tgacagctta tcatcgcaga tccgggcaac
     1861 gttgttgcca ttgctgcagg cgcagaactg gtaggtatgg aagatctata cattgaatca
     1921 atattggcaa ttagccatat tagtcattgg ttatatagca taaatcaata ttggctattg
     1981 gccattgcat acgttgtatc tatatcataa tatgtacatt tatattggct catgtccaat
     2041 atgaccgcca tgttgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc
     2101 attagttcat agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc
     2161 tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt
     2221 aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca
     2281 cttggcagta catcaagtgt atcatatgcc aagtccgccc cctattgacg tcaatgacgg
     2341 taaatggccc gcctggcatt atgcccagta catgacctta cgggactttc ctacttggca
     2401 gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc agtacaccaa
     2461 tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa
     2521 tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta ataaccccgc
     2581 cccgttgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg
     2641 tttagtgaac cgtcagatct ctagaagctg ggtaccagct gctagcgcca ccatgcccat
     2701 gggttccctc caaccacttg caacgcttta tctgctcggc atgcttgtag cgtcagtgct
     2761 tgcaaatctc gacctttggg tcaccgtata ctacggagtc cccgtgtgga aagaggctaa
     2821 gacgaccctg ttttgtgcgt cagacgcgaa agcgtatgac aaggaagtgc ataacgtgtg
     2881 ggcaacacac gcatgtgttc cgacggaccc taacccgcag gaaatcgtcc ttgaaaacgt
     2941 gaccgaaaac ttcaacatgt ggaagaacga catggttgac caaatgcacg aggacataat
     3001 cagtctttgg gatcaatcct tgaagccctg tgtgaagctg acgcctttgt gcgtaacttt
     3061 gaactgtaaa aacgtaaata tttcagcgaa tgctaacgcg accgctacac ttaattcaag
     3121 tatgaacggc gaaataaaga attgctcctt caacaccacg acagaactta gagataaaaa
     3181 gcaaaaagtc tacgcccttt tctataaacc ggatgtcgtg ccacttaacg gtggtgagca
     3241 caatgagaca ggtgagtaca ttctcatcaa ctgcaattca tctacaataa cgcaagcatg
     3301 tccgaaagtt tccttcgatc cgatccctat tcactactgc gctcctgcag ggtacgccat
     3361 actgaagtgt aacaataaaa cgtttaacgg aacaggccct tgcaataatg taagtactgt
     3421 ccagtgcacg catggcataa aacccgtcgt gagcacacaa cttcttctta acggatcact
     3481 cgccgaggaa gagatcatcg tgcgaagtga gaacttgacg aataatataa agacgatcat
     3541 agtccatctc aacaagtccg tcgagattaa atgcactagg cccaacaaca atacgcggaa
     3601 atccgtacgg ataggtcctg gacaaacatt ctatgctacc ggtgagatta taggtgatat
     3661 acgcgaagct cattgcaata tcagtaggga gacctggaat tccactttga tccaagtgaa
     3721 agaaaaactg cgggagcatt acaacaaaac gatcaaattt gaaccttctt ctggaggtga
     3781 cctggaggtt acgacgcaca gttttaactg taggggagaa ttcttctact gcgatacgac
     3841 caagctgttc aacgagacta agctgtttaa cgagtcagaa tacgttgaca ataagactat
     3901 tatcttgccc tgccggataa agcaaattat caatatgtgg caagaagtgg gaagggcaat
     3961 gtacgcgccg ccgattgaag ggaatattac ttgcaagagc aacataactg gtcttcttct
     4021 cacatgggat ggtggagaga acagtaccga aggcgttttt aggcctggcg gtggcaatat
     4081 gaaggataac tggcgctcag aactctataa gtacaaagtc gtcgaaataa agcccttggg
     4141 cgttgcgcct acgaaaagta aacgaaaagt agtagggagg gaaaagcgag cggttggctt
     4201 gggtgcagtg cttctcgggt tcctgggagc tgcggggtca acaatgggag ctgcttccat
     4261 aaccttgact gtgcaggcaa ggcagctgct gagtgggata gtgcagcaac aatccaatct
     4321 gcttagggca attgaagctc agcaacatct tttgcagctc acagtctggg gcataaagca
     4381 actgcaaacc agagtcctcg cgattgagcg gtatcttaag gaccagcaac tgctgggact
     4441 ctgggggtgt tccgggaagc ttatttgtgc cactgcggta ccatggaact cctcttggtc
     4501 caacaagtct ctgggcgata tttgggacaa tatgacgtgg atgcagtggg atcgcgagat
     4561 atccaactac accaacacga tcttccggct tctcgaagac agtcagaatc aacaagagaa
     4621 gaatgagaag gatctgttgg cccttgacag ctggaaaaat ctttggaatt ggtttgacat
     4681 tactaactgg ctgtggtaca taaaaatctt cataatgatt gtcggtggcc ttatcggtct
     4741 ccgcatcata ttcggggtat tggctatagt caaaagggta cgccaggggt atagcccgtt
     4801 gagtttccaa acgctcattc cgaatccgcg aggtcctgat cgcttgggac ggatcgaaga
     4861 ggaaggaggt gaacaagaca aggatcgatc catacgactg gtctctgggt ttcttgccct
     4921 cgcgtgggac gacctcagat cactgtgcct gttctcttat catcaattga gggacttcat
     4981 actgaccgca gcaagagccg cagagctgct gggtcgcagt tccctgcgag gtcttcagcg
     5041 aggttgggaa gtactcaagt acctcggaaa tctggtccaa tactggggtc tcgaactgaa
     5101 gcgatccgcg ataaacctct ttgacacaat agcaattgcg gtggccgagg gaacggaccg
     5161 aataatagaa gtcatacagc gcatttgcag ggctatccga tatatcccaa cccgaattcg
     5221 acaaggtttc gaagccgcat tgttgtagta actcgaggcc ggcaaggccg gatccagaca
     5281 tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct
     5341 ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac
     5401 aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg
     5461 ttttttaaag caagtaaaac ctctacaaat gtggtatggc tgattatgat ccggctgcct
     5521 cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac
     5581 agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt
     5641 tggcgggtgt cggggcgcag ccatgaggtc gactctaga
//
