LOCUS       sequence-20259-e         687 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 687)
  AUTHORS   Ma JB, Yuan YR, Meister G, Pei Y, Tuschl T, Patel DJ
  TITLE     Structural basis for 5'-end-specific recognition of guide RNA by the
            A. fulgidus Piwi protein.
  JOURNAL   Nature. 2005 Mar 31. 434(7033):666-70.
  PUBMED    15800629
REFERENCE   2  (bases 1 to 687)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2005 Mar 31. 434(7033):666-70."
COMMENT     Sequence Label: pDESTFLAGHApuro3-Ago2K570A
FEATURES             Location/Qualifiers
     source          1..687
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      37..46
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             46..69
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             70..96
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    112..136
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gatccatccg atagtggtgg tggaattctg atcgccgccg ccatggacta caaggacgac
       61 gatgacaagt acccttatga cgtgcccgat tacgctttga accagatatc aacaagtttg
      121 tacaaaaaag caggctccac catgggtgtt ctctctgcca ttcccgcact tgcacctcct
      181 gcgccgccgc cccccatcca aggatatgcc ttcaagcctc cacctagacc cgactttggg
      241 acctccggga gaacaatcaa attacaggcc aatttcttcg aaatggacat ccccaaaatt
      301 gacatctatc attatgaatt ggatatcaag ccagagaagt gcccgaggag agttaacagg
      361 gaaatcgtgg aacacatggt ccagcacttt aaaacacaga tctttgggga tcggaagccc
      421 gtgtttgacg gcaggaagaa tctatacaca gccatgcccc ttccgattgg gagggacaag
      481 gtggagctgg aggtcacgct gccaggagaa ggcaaggatc gcatcttcaa ggtgtccatc
      541 aagtgggtgt cctgcgtgag cttgcaggcg ttacacgatg cactttcagg gcggctgccc
      601 agcgtccctt ttgagacgat ccaggccctg gacgtggtca tgaggcactt gccatccatg
      661 aggtacaccc ccgtgggccg ctccttc
//
