LOCUS       sequence-202592-        5676 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression plasmid for Env from the BaL HIV-1 isolate; QES
            mutant with enhanced binding to PG16.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5676)
  AUTHORS   Heredia JD, Park J, Choi H, Gill KS, Procko E
  TITLE     Conformational engineering of HIV-1 Env based on mutational 
            tolerance in the CD4 and PG16 bound states.
  JOURNAL   J Virol. 2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19.
  PUBMED    30894475
REFERENCE   2  (bases 1 to 5676)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19."
COMMENT     Sequence Label: pCEP4-BaLgp160-QES.02
FEATURES             Location/Qualifiers
     source          1..5676
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        86..231
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             274..1311
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(1309..1328)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    1353..1401
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        2055..2434
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2435..2638
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2589..2609
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2635..2659
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     polyA_signal    5268..5402
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5292..5311)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5346..5365
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(5545..5567)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 gccctgcttc atccccgtgg cccgttgctc gcgtttgctg gcggtgtccc cggaagaaat
       61 atatttgcat gtctttagtt ctatgatgac acaaaccccg cccagcgtct tgtcattggc
      121 gaattcgaac acgcagatgc agtcggggcg gcgcggtccg aggtccactt cgcatattaa
      181 ggtgacgcgt gtggcctcga acaccgagcg accctgcagc gacccgctta acagcgtcaa
      241 cagcgtgccg cagatcccgg ggggcaatga gatatgaaaa agcctgaact caccgcgacg
      301 tctgtcgaga agtttctgat cgaaaagttc gacagcgtct ccgacctgat gcagctctcg
      361 gagggcgaag aatctcgtgc tttcagcttc gatgtaggag ggcgtggata tgtcctgcgg
      421 gtaaatagct gcgccgatgg tttctacaaa gatcgttatg tttatcggca ctttgcatcg
      481 gccgcgctcc cgattccgga agtgcttgac attggggaat tcagcgagag cctgacctat
      541 tgcatctccc gccgtgcaca gggtgtcacg ttgcaagacc tgcctgaaac cgaactgccc
      601 gctgttctgc agccggtcgc ggaggccatg gatgcgatcg ctgcggccga tcttagccag
      661 acgagcgggt tcggcccatt cggaccgcaa ggaatcggtc aatacactac atggcgtgat
      721 ttcatatgcg cgattgctga tccccatgtg tatcactggc aaactgtgat ggacgacacc
      781 gtcagtgcgt ccgtcgcgca ggctctcgat gagctgatgc tttgggccga ggactgcccc
      841 gaagtccggc acctcgtgca cgcggatttc ggctccaaca atgtcctgac ggacaatggc
      901 cgcataacag cggtcattga ctggagcgag gcgatgttcg gggattccca atacgaggtc
      961 gccaacatct tcttctggag gccgtggttg gcttgtatgg agcagcagac gcgctacttc
     1021 gagcggaggc atccggagct tgcaggatcg ccgcggctcc gggcgtatat gctccgcatt
     1081 ggtcttgacc aactctatca gagcttggtt gacggcaatt tcgatgatgc agcttgggcg
     1141 cagggtcgat gcgacgcaat cgtccgatcc ggagccggga ctgtcgggcg tacacaaatc
     1201 gcccgcagaa gcgcggccgt ctggaccgat ggctgtgtag aagtactcgc cgatagtgga
     1261 aaccgacgcc ccagcactcg tccggatcgg gagatggggg aggctaactg aaacacggaa
     1321 ggagacaata ccggaaggaa cccgcgctat gacggcaata aaaagacaga ataaaacgca
     1381 cgggtgttgg gtcgtttgtt cataaacgcg gggttcggtc ccagggctgg cactctgtcg
     1441 ataccccacc gagaccccat tggggccaat acgcccgcgt ttcttccttt tccccacccc
     1501 accccccaag ttcgggtgaa ggcccagggc tcgcagccaa cgtcggggcg gcaggccctg
     1561 ccatagccac tggccccgtg ggttagggac ggggtccccc atggggaatg gtttatggtt
     1621 cgtgggggtt attattttgg gcgttgcgtg gggtcaggtc cacgactgga ctgagcagac
     1681 agacccatgg tttttggatg gcctgggcat ggaccgcatg tactggcgcg acacgaacac
     1741 cgggcgtctg tggctgccaa acacccccga cccccaaaaa ccaccgcgcg gatttctggc
     1801 gtgccaagct agtcgaccaa ttctcatgtt tgacagctta tcatcgcaga tccgggcaac
     1861 gttgttgcca ttgctgcagg cgcagaactg gtaggtatgg aagatctata cattgaatca
     1921 atattggcaa ttagccatat tagtcattgg ttatatagca taaatcaata ttggctattg
     1981 gccattgcat acgttgtatc tatatcataa tatgtacatt tatattggct catgtccaat
     2041 atgaccgcca tgttgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc
     2101 attagttcat agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc
     2161 tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt
     2221 aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca
     2281 cttggcagta catcaagtgt atcatatgcc aagtccgccc cctattgacg tcaatgacgg
     2341 taaatggccc gcctggcatt atgcccagta catgacctta cgggactttc ctacttggca
     2401 gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc agtacaccaa
     2461 tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa
     2521 tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta ataaccccgc
     2581 cccgttgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg
     2641 tttagtgaac cgtcagatct ctagaagctg ggtaccagct gctagcgcca ccatgcccat
     2701 ggggagcttg caaccgctcg ccactctcta tctcctcggc atgttggtag catctgtgct
     2761 ggctgaggaa aagctctggg tgactgtgta ttacggggtc ccagtttgga aggaggctga
     2821 taccacgctc ttctgcgcct ccgaccggaa ggcatacgac accgaggtgc ataatgtgtg
     2881 ggccacacac gcctgtgtgc ctaccgaccc caacccacag gaagtggaac tgaagaacgt
     2941 gactgagaat ttcaatatgt ggaaaaataa tatggtggag cagatgcacg aggacattat
     3001 tagcctctgg gatcagtccc ttaaaccctg cgtgaaactg acagatctgt gcgtaacgct
     3061 gaattgtact gacctccgga atgccacgaa cggaaacgac acaaatacca cgtcaagctc
     3121 ccgagggatg gtgggagggg gagaaatgaa gaactgctca ttcaatataa cgacgaatat
     3181 ccgagggaag gttcagaaag agtatgccct gttttacaaa ctggacatcg ctcctatcga
     3241 caacaacagc aataacaggt accgcctgat tagctgcaac acttctgtga ttacccaggc
     3301 ctgccccaag gtgagcttcg agcctatccc aatccactac tgcgccccag caggctttgc
     3361 catcctgaaa tgcaaggaca agaagttcaa cggcaaaggc ccatgtacaa atgtcagtac
     3421 agtacagtgc acacacggta tccgcccagt tgtctctaca caactgctgc tgaatggatc
     3481 cttggcagag gaagaggtgg tgatcagatc tgctaacttc gccgataatg ctaaagtcat
     3541 cattgttcag ctgaacgagt ctgtcgaaat caattgtact cgcccaaaca acaacacgcg
     3601 caagagcatt catatcggac cgggcagggc cttttacaca acaggggaga ttattggtga
     3661 tattagacaa gcccactgca acctcagtcg agcaaagtgg aatgatactc tcaacaaaat
     3721 tgttatcaaa ttgcgggaac agtttgggaa caagacaatc gtatttaagc actcatccgg
     3781 aggcgatcca gaaatcgtga cacactcttt taactgcggt ggagaatttt tctactgcaa
     3841 ctccacccag ctgttcaaca gtacgtggaa cgtgacagag gagtctaaca acaccgtgga
     3901 gaataatacg atcacccttc catgtcggat taagcagatc atcaatatgt ggcaggaggt
     3961 gggaagagca atgtacgcgc cgcccattag gggccagatc agatgcagtt caaatatcac
     4021 aggcctgctc ctgacacgcg acggaggccc agaggacaac aagacagagg tgtttaggcc
     4081 cggcggcggc gacatgcgag ataattggag gtctgaattg tacaagtaca aggtggtgaa
     4141 aatcgaaccc ctgggagtgg cgcctactaa ggcaaagcgc cgcgttgtgc agagagagaa
     4201 gagggcagtg ggaattgggg cggtttttct tgggttcctg ggggctgcag gtagcaccat
     4261 gggagcggcc gctatgacac tgacagtgca agccagactc ctgctgtctg gtattgtcca
     4321 acagcaaaac aatttgctcc gcgcaataga agcccagcag cacctgctcc aactgactgt
     4381 gtggggcatt aaacaattgc aggctcgcgt gctggcagtc gaacggtacc tgagggatca
     4441 acagctcctg ggcatctggg gctgctccgg gaaactcatc tgtacgacag cggtcccttg
     4501 gaatgccagc tggtccaaca aaagcctcaa caagatttgg gacaatatga cgtggattga
     4561 gtgggacagg gaaattaaca attatacctc aatcatatat tctctgattg aagagtccca
     4621 gaatcagcaa gagaagaatg agcaggaatt gttggaaaat gataaatggg caagcctgtg
     4681 gaactggttt gacataacca aatggttgtg gtatatcaaa atcttcataa tgatcgtcgg
     4741 cggactcatc gggctccgaa ttgtgttctc agtcctcagc attgtgaata gggtgcgcca
     4801 gggctattct cccctgagct tccagaccca tctgccctct agccgaggac cagataggcc
     4861 tggtgggatc gaggaagaag gcggggaacg ggacagggat aggagtggtc ctcttgttaa
     4921 cgggttcctg gcacttatat gggtggactt gagaagcctg ttcctgttct cctatcatag
     4981 gctccgggat cttctcctga tagtcatgcg gatcgtcgag ctgttggggc ttgctggcgg
     5041 ctgggaagta ctgaaatatt ggtggaatct gttgcagtat tggagccagg aacttaagaa
     5101 ctctgctgtg agtctcctta atgccacagc tgttgctgtg gccgaaggaa ccgatcgagt
     5161 tattgaagta ctgcaacgag cagttcgcgc tattctgcat atcccacgac gcattcggca
     5221 ggggctggag agagctctcc tgtagtaact cgaggccggc aaggccggat ccagacatga
     5281 taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta
     5341 tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag
     5401 ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggaggtg tgggaggttt
     5461 tttaaagcaa gtaaaacctc tacaaatgtg gtatggctga ttatgatccg gctgcctcgc
     5521 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc
     5581 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg
     5641 cgggtgtcgg ggcgcagcca tgaggtcgac tctaga
//
