LOCUS       sequence-202593-        3568 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression plasmid for Env from the BaL HIV-1 isolate; QES
            mutant with enhanced binding to PG16.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3568)
  AUTHORS   Heredia JD, Park J, Choi H, Gill KS, Procko E
  TITLE     Conformational engineering of HIV-1 Env based on mutational 
            tolerance in the CD4 and PG16 bound states.
  JOURNAL   J Virol. 2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19.
  PUBMED    30894475
REFERENCE   2  (bases 1 to 3568)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19."
COMMENT     Sequence Label: pCEP4-BaLgp160-QES.02
FEATURES             Location/Qualifiers
     source          1..3568
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     rep_origin      391..2180
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
ORIGIN
        1 ggggcagtgc atgtaatccc ttcagttggt tggtacaact tgccaactgg gccctgttcc
       61 acatgtgaca cgggggggga ccaaacacaa aggggttctc tgactgtagt tgacatcctt
      121 ataaatggat gtgcacattt gccaacactg agtggctttc atcctggagc agactttgca
      181 gtctgtggac tgcaacacaa cattgccttt atgtgtaact cttggctgaa gctcttacac
      241 caatgctggg ggacatgtac ctcccagggg cccaggaaga ctacgggagg ctacaccaac
      301 gtcaatcaga ggggcctgtg tagctaccga taagcggacc ctcaagaggg cattagcaat
      361 agtgtttata aggccccctt gttaacccta aacgggtagc atatgcttcc cgggtagtag
      421 tatatactat ccagactaac cctaattcaa tagcatatgt tacccaacgg gaagcatatg
      481 ctatcgaatt agggttagta aaagggtcct aaggaacagc gatatctccc accccatgag
      541 ctgtcacggt tttatttaca tggggtcagg attccacgag ggtagtgaac cattttagtc
      601 acaagggcag tggctgaaga tcaaggagcg ggcagtgaac tctcctgaat cttcgcctgc
      661 ttcttcattc tccttcgttt agctaataga ataactgctg agttgtgaac agtaaggtgt
      721 atgtgaggtg ctcgaaaaca aggtttcagg tgacgccccc agaataaaat ttggacgggg
      781 ggttcagtgg tggcattgtg ctatgacacc aatataaccc tcacaaaccc cttgggcaat
      841 aaatactagt gtaggaatga aacattctga atatctttaa caatagaaat ccatggggtg
      901 gggacaagcc gtaaagactg gatgtccatc tcacacgaat ttatggctat gggcaacaca
      961 taatcctagt gcaatatgat actggggtta ttaagatgtg tcccaggcag ggaccaagac
     1021 aggtgaacca tgttgttaca ctctatttgt aacaagggga aagagagtgg acgccgacag
     1081 cagcggactc cactggttgt ctctaacacc cccgaaaatt aaacggggct ccacgccaat
     1141 ggggcccata aacaaagaca agtggccact cttttttttg aaattgtgga gtgggggcac
     1201 gcgtcagccc ccacacgccg ccctgcggtt ttggactgta aaataagggt gtaataactt
     1261 ggctgattgt aaccccgcta accactgcgg tcaaaccact tgcccacaaa accactaatg
     1321 gcaccccggg gaatacctgc ataagtaggt gggcgggcca agataggggc gcgattgctg
     1381 cgatctggag gacaaattac acacacttgc gcctgagcgc caagcacagg gttgttggtc
     1441 ctcatattca cgaggtcgct gagagcacgg tgggctaatg ttgccatggg tagcatatac
     1501 tacccaaata tctggatagc atatgctatc ctaatctata tctgggtagc ataggctatc
     1561 ctaatctata tctgggtagc atatgctatc ctaatctata tctgggtagt atatgctatc
     1621 ctaatttata tctgggtagc ataggctatc ctaatctata tctgggtagc atatgctatc
     1681 ctaatctata tctgggtagt atatgctatc ctaatctgta tccgggtagc atatgctatc
     1741 ctaatagaga ttagggtagt atatgctatc ctaatttata tctgggtagc atatactacc
     1801 caaatatctg gatagcatat gctatcctaa tctatatctg ggtagcatat gctatcctaa
     1861 tctatatctg ggtagcatag gctatcctaa tctatatctg ggtagcatat gctatcctaa
     1921 tctatatctg ggtagtatat gctatcctaa tttatatctg ggtagcatag gctatcctaa
     1981 tctatatctg ggtagcatat gctatcctaa tctatatctg ggtagtatat gctatcctaa
     2041 tctgtatccg ggtagcatat gctatcctca tgcatataca gtcagcatat gatacccagt
     2101 agtagagtgg gagtgctatc ctttgcatat gccgccacct cccaaggggg cgtgaatttt
     2161 cgctgcttgt ccttttcctg ctggttgctc ccattcttag gtgaatttaa ggaggccagg
     2221 ctaaagccgt cgcatgtctg attgctcacc aggtaaatgt cgctaatgtt ttccaacgcg
     2281 agaaggtgtt gagcgcggag ctgagtgacg tgacaacatg ggtatgccca attgccccat
     2341 gttgggagga cgaaaatggt gacaagacag atggccagaa atacaccaac agcacgcatg
     2401 atgtctactg gggatttatt ctttagtgcg ggggaataca cggcttttaa tacgattgag
     2461 ggcgtctcct aacaagttac atcactcctg cccttcctca ccctcatctc catcacctcc
     2521 ttcatctccg tcatctccgt catcaccctc cgcggcagcc ccttccacca taggtggaaa
     2581 ccagggaggc aaatctactc catcgtcaaa gctgcacaca gtcaccctga tattgcaggt
     2641 aggagcgggc tttgtcataa caaggtcctt aatcgcatcc ttcaaaacct cagcaaatat
     2701 atgagtttgt aaaaagacca tgaaataaca gacaatggac tcccttagcg ggccaggttg
     2761 tgggccgggt ccaggggcca ttccaaaggg gagacgactc aatggtgtaa gacgacattg
     2821 tggaatagca agggcagttc ctcgccttag gttgtaaagg gaggtcttac tacctccata
     2881 tacgaacaca ccggcgaccc aagttccttc gtcggtagtc ctttctacgt gactcctagc
     2941 caggagagct cttaaacctt ctgcaatgtt ctcaaatttc gggttggaac ctccttgacc
     3001 acgatgcttt ccaaaccacc ctcctttttt gcgcctgcct ccatcaccct gaccccgggg
     3061 tccagtgctt gggccttctc ctgggtcatc tgcggggccc tgctctatcg ctcccggggg
     3121 cacgtcaggc tcaccatctg ggccaccttc ttggtggtat tcaaaataat cggcttcccc
     3181 tacagggtgg aaaaatggcc ttctacctgg agggggcctg cgcggtggag acccggatga
     3241 tgatgactga ctactgggac tcctgggcct cttttctcca cgtccacgac ctctccccct
     3301 ggctctttca cgacttcccc ccctggctct ttcacgtcct ctaccccggc ggcctccact
     3361 acctcctcga ccccggcctc cactacctcc tcgaccccgg cctccactgc ctcctcgacc
     3421 ccggcctcca cctcctgctc ctgcccctcc tgctcctgcc cctcctcctg ctcctgcccc
     3481 tcctgcccct cctgctcctg cccctcctgc ccctcctgct cctgcccctc ctgcccctcc
     3541 tgctcctgcc cctcctgccc ctcctcct
//
