LOCUS       sequence-202595-       12763 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression plasmid for Env from the BaL HIV-1 isolate; QES
            mutant with enhanced binding to PG16.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12763)
  AUTHORS   Heredia JD, Park J, Choi H, Gill KS, Procko E
  TITLE     Conformational engineering of HIV-1 Env based on mutational 
            tolerance in the CD4 and PG16 bound states.
  JOURNAL   J Virol. 2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19.
  PUBMED    30894475
REFERENCE   2  (bases 1 to 12763)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19."
COMMENT     Sequence Label: pCEP4-BaLgp160-QES.01
FEATURES             Location/Qualifiers
     source          1..12763
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      475..2264
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     CDS             complement(2570..4495)
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA
                     GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG
                     GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     primer_bind     complement(4930..4948)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5016..5120
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5121..5189,5190..5981)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5339..5358)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6152..6740
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6641..6660
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        7171..7316
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     primer_bind     complement(8396..8415)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    8440..8488
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        9141..9520
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9521..9724
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9675..9695
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     9721..9745
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             11720..11764
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     polyA_signal    12354..12488
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12378..12397)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     12432..12451
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(12630..12652)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 gccccgccgc cggacgaact aaacctgact acggcatctc tgccccttct tcgctggtac
       61 gaggagcgct tttgttttgt attcggggca gtgcatgtaa tcccttcagt tggttggtac
      121 aacttgccaa ctgggccctg ttccacatgt gacacggggg gggaccaaac acaaaggggt
      181 tctctgactg tagttgacat ccttataaat ggatgtgcac atttgccaac actgagtggc
      241 tttcatcctg gagcagactt tgcatgctgt ggactgcaac acaacattgc ctttatgtgt
      301 aactcttggc tgaagctctt acaccaatgc tgggggacat gtacctccca ggggcccagg
      361 aagactacgg gaggctacac caacgtcaat cagaggggcc tgtgtagcta ccgataagcg
      421 gaccctcaag agggcattag caatagtgtt tataaggccc ccttgttaac cctaaacggg
      481 tagcatatgc ttcccgggta gtagtatata ctatccagac taaccctaat tcaatagcat
      541 atgttaccca acgggaagca tatgctatcg aattagggtt agtaaaaggg tcctaaggaa
      601 cagcgatatc tcccacccca tgagctgtca cggttttatt tacatggggt caggattcca
      661 cgagggtagt gaaccatttt agtcacaagg gcagtggctg aagatcaagg agcgggcagt
      721 gaactctcct gaatcttcgc ctgcttcttc attctccttc gtttagctaa tagaataact
      781 gctgagttgt gaacagtaag gtgtatgtga ggtgctcgaa aacaaggttt caggtgacgc
      841 ccccagaata aaatttggac ggggggttca gtggtggcat tgtgctatga caccaatata
      901 accctcacaa accccttggg caataaatac tagtgtagga atgaaacatt ctgaatatct
      961 ttaacaatag aaatccatgg ggtggggaca agccgtaaag actggatgtc catctcacac
     1021 gaatttatgg ctatgggcaa cacataatcc tagtgcaata tgatactggg gttattaaga
     1081 tgtgtcccag gcagggacca agacaggtga accatgttgt tacactctat ttgtaacaag
     1141 gggaaagaga gtggacgccg acagcagcgg actccactgg ttgtctctaa cacccccgaa
     1201 aattaaacgg ggctccacgc caatggggcc cataaacaaa gacaagtggc cactcttttt
     1261 tttgaaattg tggagtgggg gcacgcgtca gcccccacac gccgccctgc ggttttggac
     1321 tgtaaaataa gggtgtaata acttggctga ttgtaacccc gctaaccact gcggtcaaac
     1381 cacttgccca caaaaccact aatggcaccc cggggaatac ctgcataagt aggtgggcgg
     1441 gccaagatag gggcgcgatt gctgcgatct ggaggacaaa ttacacacac ttgcgcctga
     1501 gcgccaagca cagggttgtt ggtcctcata ttcacgaggt cgctgagagc acggtgggct
     1561 aatgttgcca tgggtagcat atactaccca aatatctgga tagcatatgc tatcctaatc
     1621 tatatctggg tagcataggc tatcctaatc tatatctggg tagcatatgc tatcctaatc
     1681 tatatctggg tagtatatgc tatcctaatt tatatctggg tagcataggc tatcctaatc
     1741 tatatctggg tagcatatgc tatcctaatc tatatctggg tagtatatgc tatcctaatc
     1801 tgtatccggg tagcatatgc tatcctaata gagattaggg tagtatatgc tatcctaatt
     1861 tatatctggg tagcatatac tacccaaata tctggatagc atatgctatc ctaatctata
     1921 tctgggtagc atatgctatc ctaatctata tctgggtagc ataggctatc ctaatctata
     1981 tctgggtagc atatgctatc ctaatctata tctgggtagt atatgctatc ctaatttata
     2041 tctgggtagc ataggctatc ctaatctata tctgggtagc atatgctatc ctaatctata
     2101 tctgggtagt atatgctatc ctaatctgta tccgggtagc atatgctatc ctcatgcata
     2161 tacagtcagc atatgatacc cagtagtaga gtgggagtgc tatcctttgc atatgccgcc
     2221 acctcccaag ggggcgtgaa ttttcgctgc ttgtcctttt cctgcatgct ggttgctccc
     2281 attcttaggt gaatttaagg aggccaggct aaagccgtcg catgtctgat tgctcaccag
     2341 gtaaatgtcg ctaatgtttt ccaacgcgag aaggtgttga gcgcggagct gagtgacgtg
     2401 acaacatggg tatgcccaat tgccccatgt tgggaggacg aaaatggtga caagacagat
     2461 ggccagaaat acaccaacag cacgcatgat gtctactggg gatttattct ttagtgcggg
     2521 ggaatacacg gcttttaata cgattgaggg cgtctcctaa caagttacat cactcctgcc
     2581 cttcctcacc ctcatctcca tcacctcctt catctccgtc atctccgtca tcaccctccg
     2641 cggcagcccc ttccaccata ggtggaaacc agggaggcaa atctactcca tcgtcaaagc
     2701 tgcacacagt caccctgata ttgcaggtag gagcgggctt tgtcataaca aggtccttaa
     2761 tcgcatcctt caaaacctca gcaaatatat gagtttgtaa aaagaccatg aaataacaga
     2821 caatggactc ccttagcggg ccaggttgtg ggccgggtcc aggggccatt ccaaagggga
     2881 gacgactcaa tggtgtaaga cgacattgtg gaatagcaag ggcagttcct cgccttaggt
     2941 tgtaaaggga ggtcttacta cctccatata cgaacacacc ggcgacccaa gttccttcgt
     3001 cggtagtcct ttctacgtga ctcctagcca ggagagctct taaaccttct gcaatgttct
     3061 caaatttcgg gttggaacct ccttgaccac gatgctttcc aaaccaccct ccttttttgc
     3121 gcctgcctcc atcaccctga ccccggggtc cagtgcttgg gccttctcct gggtcatctg
     3181 cggggccctg ctctatcgct cccgggggca cgtcaggctc accatctggg ccaccttctt
     3241 ggtggtattc aaaataatcg gcttccccta cagggtggaa aaatggcctt ctacctggag
     3301 ggggcctgcg cggtggagac ccggatgatg atgactgact actgggactc ctgggcctct
     3361 tttctccacg tccacgacct ctccccctgg ctctttcacg acttcccccc ctggctcttt
     3421 cacgtcctct accccggcgg cctccactac ctcctcgacc ccggcctcca ctacctcctc
     3481 gaccccggcc tccactgcct cctcgacccc ggcctccacc tcctgctcct gcccctcctg
     3541 ctcctgcccc tcctcctgct cctgcccctc ctgcccctcc tgctcctgcc cctcctgccc
     3601 ctcctgctcc tgcccctcct gcccctcctg ctcctgcccc tcctgcccct cctcctgctc
     3661 ctgcccctcc tgcccctcct cctgctcctg cccctcctgc ccctcctgct cctgcccctc
     3721 ctgcccctcc tgctcctgcc cctcctgccc ctcctgctcc tgcccctcct gctcctgccc
     3781 ctcctgctcc tgcccctcct gctcctgccc ctcctgcccc tcctgcccct cctcctgctc
     3841 ctgcccctcc tgctcctgcc cctcctgccc ctcctgcccc tcctgctcct gcccctcctc
     3901 ctgctcctgc ccctcctgcc cctcctgccc ctcctcctgc tcctgcccct cctgcccctc
     3961 ctcctgctcc tgcccctcct cctgctcctg cccctcctgc ccctcctgcc cctcctcctg
     4021 ctcctgcccc tcctgcccct cctcctgctc ctgcccctcc tcctgctcct gcccctcctg
     4081 cccctcctgc ccctcctcct gctcctgccc ctcctcctgc tcctgcccct cctgcccctc
     4141 ctgcccctcc tgcccctcct cctgctcctg cccctcctcc tgctcctgcc cctcctgctc
     4201 ctgcccctcc cgctcctgct cctgctcctg ttccaccgtg ggtccctttg cagccaatgc
     4261 aacttggacg tttttggggt ctccggacac catctctatg tcttggccct gatcctgagc
     4321 cgcccggggc tcctggtctt ccgcctcctc gtcctcgtcc tcttccccgt cctcgtccat
     4381 ggttatcacc ccctcttctt tgaggtccac tgccgccgga gccttctggt ccagatgtgt
     4441 ctcccttctc tcctaggcca tttccaggtc ctgtacctgg cccctcgtca gacatgattc
     4501 acactaaaag agatcaatag acatctttat tagacgacgc tcagtgaata cagggagtgc
     4561 agactcctgc cccctccaac agccccccca ccctcatccc cttcatggtc gctgtcagac
     4621 agatccaggt ctgaaaattc cccatcctcc gaaccatcct cgtcctcatc accaattact
     4681 cgcagcccgg aaaactcccg ctgaacatcc tcaagatttg cgtcctgagc ctcaagccag
     4741 gcctcaaatt cctcgtcccc ctttttgctg gacggtaggg atggggattc tcgggacccc
     4801 tcctcttcct cttcaaggtc accagacaga gatgctactg gggcaacgga agaaaagctg
     4861 ggtgcggcct gtgaggatca gcttatcgat gataagctgt caaacatgag aattcttgaa
     4921 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
     4981 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     5041 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     5101 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     5161 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     5221 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     5281 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     5341 tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac
     5401 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     5461 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     5521 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     5581 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     5641 acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg
     5701 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     5761 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     5821 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     5881 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     5941 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     6001 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     6061 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     6121 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     6181 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     6241 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
     6301 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     6361 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     6421 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     6481 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     6541 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     6601 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     6661 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     6721 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     6781 gctggccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca
     6841 acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc
     6901 ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccccgaga tgcgccgcgt
     6961 gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt
     7021 cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag
     7081 gtgccgccct gcttcatccc cgtggcccgt tgctcgcgtt tgctggcggt gtccccggaa
     7141 gaaatatatt tgcatgtctt tagttctatg atgacacaaa ccccgcccag cgtcttgtca
     7201 ttggcgaatt cgaacacgca gatgcagtcg gggcggcgcg gtccgaggtc cacttcgcat
     7261 attaaggtga cgcgtgtggc ctcgaacacc gagcgaccct gcagcgaccc gcttaacagc
     7321 gtcaacagcg tgccgcagat cccggggggg caatgagata tgaaaaagcc tgaactcacc
     7381 gcgacgtctg tcgagaagtt tctgatcgaa aagtttcgac agcgtctccg acctgatgca
     7441 gctctcggag ggcgaagaat ctcgtgcttt cagcttcgat gtaggagggc gtggatatgt
     7501 cctgcgggta aatagctgcg ccgatggttt ctacaaagat cgttatgttt atcggcactt
     7561 tgcatcggcc gcgctcccga ttccggaagt gcttgacatt ggggaattca gcgagagcct
     7621 gacctattgc atctcccgcc gtgcacaggg tgtcacgttg caagacctgc ctgaaaccga
     7681 actgcccgct gttctgcagc cggtcgcgga ggccatggat gcgatcgctg cggccgatct
     7741 tagccagacg agcgggttcg gcccattcgg accgcaagga atcggtcaat acactacatg
     7801 gcgtgatttc atatgcgcga ttgctgatcc ccatgtgtat cactggcaaa ctgtgatgga
     7861 cgacaccgtc agtgcgtccg tcgcgcaggc tctcgatgag ctgatgcttt gggccgagga
     7921 ctgccccgaa gtccggcacc tcgtgcacgc ggatttcggc tccaacaatg tcctgacgga
     7981 caatggccgc ataacagcgg tcattgactg gagcgaggcg atgttcgggg attcccaata
     8041 cgaggtcgcc aacatcttct tctggaggcc gtggttggct tgtatggagc agcagacgcg
     8101 ctacttcgag cggaggcatc cggagcttgc aggatcgccg cggctccggg cgtatatgct
     8161 ccgcattggt cttgaccaac tctatcagag cttggttgac ggcaatttcg atgatgcagc
     8221 ttgggcgcag ggtcgatgcg acgcaatcgt ccgatccgga gccgggactg tcgggcgtac
     8281 acaaatcgcc cgcagaagcg cggccgtctg gaccgatggc tgtgtagaag tactcgccga
     8341 tagtggaaac cgacgcccca gcactcgtcc ggatcgggag atgggggagg ctaactgaaa
     8401 cacggaagga gacaataccg gaaggaaccc gcgctatgac ggcaataaaa agacagaata
     8461 aaacgcacgg gtgttgggtc gtttgttcat aaacgcgggg ttcggtccca gggctggcac
     8521 tctgtcgata ccccaccgag accccattgg ggccaatacg cccgcgtttc ttccttttcc
     8581 ccaccccacc ccccaagttc gggtgaaggc ccagggctcg cagccaacgt cggggcggca
     8641 ggccctgcca tagccactgg ccccgtgggt tagggacggg gtcccccatg gggaatggtt
     8701 tatggttcgt gggggttatt attttgggcg ttgcgtgggg tctggtccac gactggactg
     8761 agcagacaga cccatggttt ttggatggcc tgggcatgga ccgcatgtac tggcgcgaca
     8821 cgaacaccgg gcgtctgtgg ctgccaaaca cccccgaccc ccaaaaacca ccgcgcggat
     8881 ttctggcgtg ccaagctagt cgaccaattc tcatgtttga cagcttatca tcgcagatcc
     8941 gggcaacgtt gttgcattgc tgcaggcgca gaactggtag gtatggaaga tctatacatt
     9001 gaatcaatat tggcaattag ccatattagt cattggttat atagcataaa tcaatattgg
     9061 ctattggcca ttgcatacgt tgtatctata tcataatatg tacatttata ttggctcatg
     9121 tccaatatga ccgccatgtt gacattgatt attgactagt tattaatagt aatcaattac
     9181 ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg
     9241 cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc
     9301 catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac
     9361 tgcccacttg gcagtacatc aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa
     9421 tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttacggg actttcctac
     9481 ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta
     9541 caccaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga
     9601 cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaataa
     9661 ccccgccccg ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag
     9721 agctcgttta gtgaaccgtc agatctctag aagctgggta ccagctgcta gcgccaccat
     9781 gcccatgggg agcttgcaac cgctcgccac tctctatctc ctcggcatgt tggtagcatc
     9841 tgtgctggct gaggaaaagc tctgggtgac tgtgtattac ggggtcccag tttggaagga
     9901 ggctgatacc acgctcttct gcgcctccga ccggaaggca tacgacaccg aggtgcataa
     9961 tgtgtgggcc acacacgcct gtgtgcctac cgaccccaac ccacaggaag tggaactgaa
    10021 gaacgtgact gagaatttca atatgtggaa aaataatatg gtggagcaga tgcacgagga
    10081 cattattagc ctctgggatc agtcccttaa accctgcgtg aaactgacag atctgtgcgt
    10141 aacgctgaat tgtactgacc tccggaatgc cacgaacgga aacgacacaa ataccacgtc
    10201 aagctcccga gggatggtgg gagggggaga aatgaagaac tgctcattca atataacgac
    10261 gaatatccga gggaaggttc agaaagagta tgccctgttt tacaaactgg acatcgctcc
    10321 tatcgacaac aacagcaata acaggtaccg cctgattagc tgcaacactt ctgtgattac
    10381 ccaggcctgc cccaaggtga gcttcgagcc tatcccaatc cactactgcg ccccagcagg
    10441 ctttgccatc ctgaaatgca aggacaagaa gttcaacggc aaaggcccat gtacaaatgt
    10501 cagtacagta cagtgcacac acggtatccg cccagttgtc tctacacaac tgctgctgaa
    10561 tggatccttg gcagaggaag aggtggtgat cagatctgct aacttcgccg ataatgctaa
    10621 agtcatcatt gttcagctga acgagtctgt cgaaatcaat tgtactcgcc caaacaacaa
    10681 cacgcgcaag agcattcata tcggaccggg cagggccttt tacacaacag gggagattat
    10741 tggtgatatt agacaagccc actgcaacct cagtcgagca aagtggaatg atactctcaa
    10801 caaaattgtt atcaaattgc gggaacagtt tgggaacaag acaatcgtat ttaagcactc
    10861 atccggaggc gatccagaaa tcgtgacaca ctcttttaac tgcggtggag aatttttcta
    10921 ctgcaactcc acccagctgt tcaacagtac gtggaacgtg acagaggagt ctaacaacac
    10981 cgtggagaat aatacgatca cccttccatg tcggattaag cagatcatca atatgtggca
    11041 ggaggtggga agagcaatgt acgcgccgcc cattaggggc cagatcagat gcagttcaaa
    11101 tatcacaggc ctgctcctga cacgcgacgg aggcccagag gacaacaaga cagaggtgtt
    11161 taggcccggc ggcggcgaca tgcgagataa ttggaggtct gaattgtaca agtacaaggt
    11221 ggtgaaaatc gaacccctgg gagtggcgcc tactaaggca aagcgccgcg ttgtgcagag
    11281 agagaagagg gcagtgggaa ttggggcggt ttttcttggg ttcctggggg ctgcaggtag
    11341 caccatggga gcggccgcta tgacactgac agtgcaagcc agactcctgc tgtctggtat
    11401 tgtccaacag caaaacaatt tgctccgcgc aatagaagcc cagcagcacc tgctccaact
    11461 gactgtgtgg ggcattaaac aattgcaggc tcgcgtgctg gcagtcgaac ggtacctgag
    11521 ggatcaacag ctcctgggca tgtggggctg ctccgggaaa ctcatctgta cgacagcggt
    11581 cccttggaat gccagctggt ccaacaaaag cctcaacaag atttgggaca atatgacgtg
    11641 gattgagtgg gacagggaaa ttaacaatta tacctcaatc atatattctc tgattgaaga
    11701 gtcccagaat cagcaagaga agaatgagca ggaattgttg gaacttgata aatgggcaag
    11761 cctgtggaac tggtttgaca taaccaaatg gttgtggtat atcaaaatct tcataatgat
    11821 cgtcggcgga ctcatcgggc tccgaattgt gttctcagtc ctcagcattg tgaatagggt
    11881 gcgccagggc tattctcccc tgagcttcca gacccatctg ccctctagcc gaggaccaga
    11941 taggcctggt gggatcgagg aagaaggcgg ggaacgggac agggatagga gtggtcctct
    12001 tgttaacggg ttcctggcac ttatatgggt ggacttgaga agcctgttcc tgttctccta
    12061 tcataggctc cgggatcttc tcctgatagt catgcggatc gtcgagctgt tggggcttgc
    12121 tggcggctgg gaagtactga aatattggtg gaatctgttg cagtattgga gccaggaact
    12181 taagaactct gctgtgagtc tccttaatgc cacagctgtt gctgtggccg aaggaaccga
    12241 tcgagttatt gaagtactgc aacgagcagt tcgcgctatt ctgcatatcc cacgacgcat
    12301 tcggcagggg ctggagagag ctctcctgta gtaactcgag gccggcaagg ccggatccag
    12361 acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat
    12421 gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata
    12481 aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgggga
    12541 ggttttttaa agcaagtaaa acctctacaa atgtggtatg gctgattatg atccggctgc
    12601 ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc
    12661 acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt
    12721 gttggcgggt gtcggggcgc agccatgacc ggtcgactct aga
//
