LOCUS       sequence-202597-        7748 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression plasmid for Env from the BaL HIV-1 isolate; QES
            mutant with enhanced binding to PG16.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 7748)
  AUTHORS   Heredia JD, Park J, Choi H, Gill KS, Procko E
  TITLE     Conformational engineering of HIV-1 Env based on mutational 
            tolerance in the CD4 and PG16 bound states.
  JOURNAL   J Virol. 2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19.
  PUBMED    30894475
REFERENCE   2  (bases 1 to 7748)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19."
COMMENT     Sequence Label: pCEP4-BaLgp160-QES.01
FEATURES             Location/Qualifiers
     source          1..7748
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        1..105
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(106..174,175..966)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(324..343)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1137..1725
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1626..1645
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        2156..2301
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     primer_bind     complement(3381..3400)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    3425..3473
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        4127..4506
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4507..4710
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4661..4681
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4707..4731
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             6706..6750
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     polyA_signal    7340..7474
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7364..7383)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7418..7437
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(7617..7639)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
       61 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
      121 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
      181 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
      241 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
      301 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg
      361 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
      421 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
      481 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
      541 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
      601 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca
      661 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
      721 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
      781 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
      841 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
      901 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
      961 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     1021 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     1081 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     1141 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     1201 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     1261 agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag
     1321 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     1381 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     1441 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     1501 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     1561 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     1621 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     1681 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     1741 gcctttttac ggttcctggc cttttgctgg ccttgaagct gtccctgatg gtcgtcatct
     1801 acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga
     1861 atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc
     1921 gcgtcggccc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt
     1981 tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc
     2041 ttggagtggt gaatccgtta gcgaggtgcc gccctgcttc atccccgtgg cccgttgctc
     2101 gcgtttgctg gcggtgtccc cggaagaaat atatttgcat gtctttagtt ctatgatgac
     2161 acaaaccccg cccagcgtct tgtcattggc gaattcgaac acgcagatgc agtcggggcg
     2221 gcgcggtccg aggtccactt cgcatattaa ggtgacgcgt gtggcctcga acaccgagcg
     2281 accctgcagc gacccgctta acagcgtcaa cagcgtgccg cagatcccgg gggggcaatg
     2341 agatatgaaa aagcctgaac tcaccgcgac gtctgtcgag aagtttctga tcgaaaagtt
     2401 tcgacagcgt ctccgacctg atgcagctct cggagggcga agaatctcgt gctttcagct
     2461 tcgatgtagg agggcgtgga tatgtcctgc gggtaaatag ctgcgccgat ggtttctaca
     2521 aagatcgtta tgtttatcgg cactttgcat cggccgcgct cccgattccg gaagtgcttg
     2581 acattgggga attcagcgag agcctgacct attgcatctc ccgccgtgca cagggtgtca
     2641 cgttgcaaga cctgcctgaa accgaactgc ccgctgttct gcagccggtc gcggaggcca
     2701 tggatgcgat cgctgcggcc gatcttagcc agacgagcgg gttcggccca ttcggaccgc
     2761 aaggaatcgg tcaatacact acatggcgtg atttcatatg cgcgattgct gatccccatg
     2821 tgtatcactg gcaaactgtg atggacgaca ccgtcagtgc gtccgtcgcg caggctctcg
     2881 atgagctgat gctttgggcc gaggactgcc ccgaagtccg gcacctcgtg cacgcggatt
     2941 tcggctccaa caatgtcctg acggacaatg gccgcataac agcggtcatt gactggagcg
     3001 aggcgatgtt cggggattcc caatacgagg tcgccaacat cttcttctgg aggccgtggt
     3061 tggcttgtat ggagcagcag acgcgctact tcgagcggag gcatccggag cttgcaggat
     3121 cgccgcggct ccgggcgtat atgctccgca ttggtcttga ccaactctat cagagcttgg
     3181 ttgacggcaa tttcgatgat gcagcttggg cgcagggtcg atgcgacgca atcgtccgat
     3241 ccggagccgg gactgtcggg cgtacacaaa tcgcccgcag aagcgcggcc gtctggaccg
     3301 atggctgtgt agaagtactc gccgatagtg gaaaccgacg ccccagcact cgtccggatc
     3361 gggagatggg ggaggctaac tgaaacacgg aaggagacaa taccggaagg aacccgcgct
     3421 atgacggcaa taaaaagaca gaataaaacg cacgggtgtt gggtcgtttg ttcataaacg
     3481 cggggttcgg tcccagggct ggcactctgt cgatacccca ccgagacccc attggggcca
     3541 atacgcccgc gtttcttcct tttccccacc ccacccccca agttcgggtg aaggcccagg
     3601 gctcgcagcc aacgtcgggg cggcaggccc tgccatagcc actggccccg tgggttaggg
     3661 acggggtccc ccatggggaa tggtttatgg ttcgtggggg ttattatttt gggcgttgcg
     3721 tggggtcagg tccacgactg gactgagcag acagacccat ggtttttgga tggcctgggc
     3781 atggaccgca tgtactggcg cgacacgaac accgggcgtc tgtggctgcc aaacaccccc
     3841 gacccccaaa aaccaccgcg cggatttctg gcgtgccaag ctagtcgacc aattctcatg
     3901 tttgacagct tatcatcgca gatccgggca acgttgttgc cattgctgca ggcgcagaac
     3961 tggtaggtat ggaagatcta tacattgaat caatattggc aattagccat attagtcatt
     4021 ggttatatag cataaatcaa tattggctat tggccattgc atacgttgta tctatatcat
     4081 aatatgtaca tttatattgg ctcatgtcca atatgaccgc catgttgaca ttgattattg
     4141 actagttatt aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc
     4201 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca
     4261 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt
     4321 caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg
     4381 ccaagtccgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag
     4441 tacatgacct tacgggactt tcctacttgg cagtacatct acgtattagt catcgctatt
     4501 accatggtga tgcggttttg gcagtacacc aatgggcgtg gatagcggtt tgactcacgg
     4561 ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa
     4621 cgggactttc caaaatgtcg taataacccc gccccgttga cgcaaatggg cggtaggcgt
     4681 gtacggtggg aggtctatat aagcagagct cgtttagtga accgtcagat ctctagaagc
     4741 tgggtaccag ctgctagcgc caccatgccc atggggagct tgcaaccgct cgccactctc
     4801 tatctcctcg gcatgttggt agcatctgtg ctggctgagg aaaagctctg ggtgactgtg
     4861 tattacgggg tcccagtttg gaaggaggct gataccacgc tcttctgcgc ctccgaccgg
     4921 aaggcatacg acaccgaggt gcataatgtg tgggccacac acgcctgtgt gcctaccgac
     4981 cccaacccac aggaagtgga actgaagaac gtgactgaga atttcaatat gtggaaaaat
     5041 aatatggtgg agcagatgca cgaggacatt attagcctct gggatcagtc ccttaaaccc
     5101 tgcgtgaaac tgacagatct gtgcgtaacg ctgaattgta ctgacctccg gaatgccacg
     5161 aacggaaacg acacaaatac cacgtcaagc tcccgaggga tggtgggagg gggagaaatg
     5221 aagaactgct cattcaatat aacgacgaat atccgaggga aggttcagaa agagtatgcc
     5281 ctgttttaca aactggacat cgctcctatc gacaacaaca gcaataacag gtaccgcctg
     5341 attagctgca acacttctgt gattacccag gcctgcccca aggtgagctt cgagcctatc
     5401 ccaatccact actgcgcccc agcaggcttt gccatcctga aatgcaagga caagaagttc
     5461 aacggcaaag gcccatgtac aaatgtcagt acagtacagt gcacacacgg tatccgccca
     5521 gttgtctcta cacaactgct gctgaatgga tccttggcag aggaagaggt ggtgatcaga
     5581 tctgctaact tcgccgataa tgctaaagtc atcattgttc agctgaacga gtctgtcgaa
     5641 atcaattgta ctcgcccaaa caacaacacg cgcaagagca ttcatatcgg accgggcagg
     5701 gccttttaca caacagggga gattattggt gatattagac aagcccactg caacctcagt
     5761 cgagcaaagt ggaatgatac tctcaacaaa attgttatca aattgcggga acagtttggg
     5821 aacaagacaa tcgtatttaa gcactcatcc ggaggcgatc cagaaatcgt gacacactct
     5881 tttaactgcg gtggagaatt tttctactgc aactccaccc agctgttcaa cagtacgtgg
     5941 aacgtgacag aggagtctaa caacaccgtg gagaataata cgatcaccct tccatgtcgg
     6001 attaagcaga tcatcaatat gtggcaggag gtgggaagag caatgtacgc gccgcccatt
     6061 aggggccaga tcagatgcag ttcaaatatc acaggcctgc tcctgacacg cgacggaggc
     6121 ccagaggaca acaagacaga ggtgtttagg cccggcggcg gcgacatgcg agataattgg
     6181 aggtctgaat tgtacaagta caaggtggtg aaaatcgaac ccctgggagt ggcgcctact
     6241 aaggcaaagc gccgcgttgt gcagagagag aagagggcag tgggaattgg ggcggttttt
     6301 cttgggttcc tgggggctgc aggtagcacc atgggagcgg ccgctatgac actgacagtg
     6361 caagccagac tcctgctgtc tggtattgtc caacagcaaa acaatttgct ccgcgcaata
     6421 gaagcccagc agcacctgct ccaactgact gtgtggggca ttaaacaatt gcaggctcgc
     6481 gtgctggcag tcgaacggta cctgagggat caacagctcc tgggcatgtg gggctgctcc
     6541 gggaaactca tctgtacgac agcggtccct tggaatgcca gctggtccaa caaaagcctc
     6601 aacaagattt gggacaatat gacgtggatt gagtgggaca gggaaattaa caattatacc
     6661 tcaatcatat attctctgat tgaagagtcc cagaatcagc aagagaagaa tgagcaggaa
     6721 ttgttggaac ttgataaatg ggcaagcctg tggaactggt ttgacataac caaatggttg
     6781 tggtatatca aaatcttcat aatgatcgtc ggcggactca tcgggctccg aattgtgttc
     6841 tcagtcctca gcattgtgaa tagggtgcgc cagggctatt ctcccctgag cttccagacc
     6901 catctgccct ctagccgagg accagatagg cctggtggga tcgaggaaga aggcggggaa
     6961 cgggacaggg ataggagtgg tcctcttgtt aacgggttcc tggcacttat atgggtggac
     7021 ttgagaagcc tgttcctgtt ctcctatcat aggctccggg atcttctcct gatagtcatg
     7081 cggatcgtcg agctgttggg gcttgctggc ggctgggaag tactgaaata ttggtggaat
     7141 ctgttgcagt attggagcca ggaacttaag aactctgctg tgagtctcct taatgccaca
     7201 gctgttgctg tggccgaagg aaccgatcga gttattgaag tactgcaacg agcagttcgc
     7261 gctattctgc atatcccacg acgcattcgg caggggctgg agagagctct cctgtagtaa
     7321 ctcgaggccg gcaaggccgg atccagacat gataagatac attgatgagt ttggacaaac
     7381 cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt
     7441 atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca ttcattttat
     7501 gtttcaggtt cagggggagg tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg
     7561 tggtatggct gattatgatc cggctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc
     7621 tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga
     7681 caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc catgaggtcg
     7741 actctaga
//
