LOCUS       sequence-20260-e         680 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 680)
  AUTHORS   Ma JB, Yuan YR, Meister G, Pei Y, Tuschl T, Patel DJ
  TITLE     Structural basis for 5'-end-specific recognition of guide RNA by the
            A. fulgidus Piwi protein.
  JOURNAL   Nature. 2005 Mar 31. 434(7033):666-70.
  PUBMED    15800629
REFERENCE   2  (bases 1 to 680)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2005 Mar 31. 434(7033):666-70."
COMMENT     Sequence Label: pDESTFLAGHApuro3-Ago2K533AQ545A
FEATURES             Location/Qualifiers
     source          1..680
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      19..28
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             28..51
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             52..78
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    94..118
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ggtggaattc tgatcgccgc cgccatggac tacaaggacg acgatgacaa gtacccttat
       61 gacgtgcccg attacgcttt gaaccagata tcaacaagtt tgtacaaaaa agcaggctcc
      121 accatgggtg ttctctctgc cattcccgca cttgcacctc ctgcgccgcc gccccccatc
      181 caaggatatg ccttcaagcc tccacctaga cccgactttg ggacctccgg gagaacaatc
      241 aaattacagg ccaatttctt cgaaatggac atccccaaaa ttgacatcta tcattatgaa
      301 ttggatatca agccagagaa gtgcccgagg agagttaaca gggaaatcgt ggaacacatg
      361 gtccagcact ttaaaacaca gatctttggg gatcggaagc ccgtgtttga cggcaggaag
      421 aatctataca cagccatgcc ccttccgatt gggagggaca aggtggagct ggaggtcacg
      481 ctgccaggag aaggcaagga tcgcatcttc aaggtgtcca tcaagtgggt gtcctgcgtg
      541 agcttgcagg cgttacacga tgcactttca gggcggctgc ccagcgtccc ttttgagacg
      601 atccaggccc tggacgtggt catgaggcac ttgccatcca tgaggtacac ccccgtgggc
      661 cgctccttct tcaccgcgtc
//
