LOCUS       sequence-202600-        7738 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression plasmid for Env from the Q842.d12 HIV-1 strain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 7738)
  AUTHORS   Heredia JD, Park J, Choi H, Gill KS, Procko E
  TITLE     Conformational engineering of HIV-1 Env based on mutational 
            tolerance in the CD4 and PG16 bound states.
  JOURNAL   J Virol. 2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19.
  PUBMED    30894475
REFERENCE   2  (bases 1 to 7738)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19."
COMMENT     Sequence Label: pCEP4-Q842gp160
FEATURES             Location/Qualifiers
     source          1..7738
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        1..105
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(106..174,175..966)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(324..343)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1137..1725
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1626..1645
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        2156..2301
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             2345..3382
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(3380..3399)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    3424..3472
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        4126..4505
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4506..4709
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4660..4680
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4706..4730
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     polyA_signal    7330..7464
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7354..7373)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7408..7427
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(7607..7629)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
       61 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
      121 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
      181 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
      241 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
      301 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg
      361 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
      421 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
      481 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
      541 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
      601 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca
      661 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
      721 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
      781 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
      841 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
      901 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
      961 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     1021 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     1081 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     1141 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     1201 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     1261 agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag
     1321 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     1381 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     1441 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     1501 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     1561 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     1621 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     1681 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     1741 gcctttttac ggttcctggc cttttgctgg ccttgaagct gtccctgatg gtcgtcatct
     1801 acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga
     1861 atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc
     1921 gcgtcggccc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt
     1981 tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc
     2041 ttggagtggt gaatccgtta gcgaggtgcc gccctgcttc atccccgtgg cccgttgctc
     2101 gcgtttgctg gcggtgtccc cggaagaaat atatttgcat gtctttagtt ctatgatgac
     2161 acaaaccccg cccagcgtct tgtcattggc gaattcgaac acgcagatgc agtcggggcg
     2221 gcgcggtccg aggtccactt cgcatattaa ggtgacgcgt gtggcctcga acaccgagcg
     2281 accctgcagc gacccgctta acagcgtcaa cagcgtgccg cagatcccgg gggggcaatg
     2341 agatatgaaa aagcctgaac tcaccgcgac gtctgtcgag aagtttctga tcgaaaagtt
     2401 cgacagcgtc tccgacctga tgcagctctc ggagggcgaa gaatctcgtg ctttcagctt
     2461 cgatgtagga gggcgtggat atgtcctgcg ggtaaatagc tgcgccgatg gtttctacaa
     2521 agatcgttat gtttatcggc actttgcatc ggccgcgctc ccgattccgg aagtgcttga
     2581 cattggggaa ttcagcgaga gcctgaccta ttgcatctcc cgccgtgcac agggtgtcac
     2641 gttgcaagac ctgcctgaaa ccgaactgcc cgctgttctg cagccggtcg cggaggccat
     2701 ggatgcgatc gctgcggccg atcttagcca gacgagcggg ttcggcccat tcggaccgca
     2761 aggaatcggt caatacacta catggcgtga tttcatatgc gcgattgctg atccccatgt
     2821 gtatcactgg caaactgtga tggacgacac cgtcagtgcg tccgtcgcgc aggctctcga
     2881 tgagctgatg ctttgggccg aggactgccc cgaagtccgg cacctcgtgc acgcggattt
     2941 cggctccaac aatgtcctga cggacaatgg ccgcataaca gcggtcattg actggagcga
     3001 ggcgatgttc ggggattccc aatacgaggt cgccaacatc ttcttctgga ggccgtggtt
     3061 ggcttgtatg gagcagcaga cgcgctactt cgagcggagg catccggagc ttgcaggatc
     3121 gccgcggctc cgggcgtata tgctccgcat tggtcttgac caactctatc agagcttggt
     3181 tgacggcaat ttcgatgatg cagcttgggc gcagggtcga tgcgacgcaa tcgtccgatc
     3241 cggagccggg actgtcgggc gtacacaaat cgcccgcaga agcgcggccg tctggaccga
     3301 tggctgtgta gaagtactcg ccgatagtgg aaaccgacgc cccagcactc gtccggatcg
     3361 ggagatgggg gaggctaact gaaacacgga aggagacaat accggaagga acccgcgcta
     3421 tgacggcaat aaaaagacag aataaaacgc acgggtgttg ggtcgtttgt tcataaacgc
     3481 ggggttcggt cccagggctg gcactctgtc gataccccac cgagacccca ttggggccaa
     3541 tacgcccgcg tttcttcctt ttccccaccc caccccccaa gttcgggtga aggcccaggg
     3601 ctcgcagcca acgtcggggc ggcaggccct gccatagcca ctggccccgt gggttaggga
     3661 cggggtcccc catggggaat ggtttatggt tcgtgggggt tattattttg ggcgttgcgt
     3721 ggggtcaggt ccacgactgg actgagcaga cagacccatg gtttttggat ggcctgggca
     3781 tggaccgcat gtactggcgc gacacgaaca ccgggcgtct gtggctgcca aacacccccg
     3841 acccccaaaa accaccgcgc ggatttctgg cgtgccaagc tagtcgacca attctcatgt
     3901 ttgacagctt atcatcgcag atccgggcaa cgttgttgcc attgctgcag gcgcagaact
     3961 ggtaggtatg gaagatctat acattgaatc aatattggca attagccata ttagtcattg
     4021 gttatatagc ataaatcaat attggctatt ggccattgca tacgttgtat ctatatcata
     4081 atatgtacat ttatattggc tcatgtccaa tatgaccgcc atgttgacat tgattattga
     4141 ctagttatta atagtaatca attacggggt cattagttca tagcccatat atggagttcc
     4201 gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat
     4261 tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc
     4321 aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc
     4381 caagtccgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt
     4441 acatgacctt acgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta
     4501 ccatggtgat gcggttttgg cagtacacca atgggcgtgg atagcggttt gactcacggg
     4561 gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac
     4621 gggactttcc aaaatgtcgt aataaccccg ccccgttgac gcaaatgggc ggtaggcgtg
     4681 tacggtggga ggtctatata agcagagctc gtttagtgaa ccgtcagatc tctagaagct
     4741 gggtaccagc tgctagcgcc accatgccaa tgggatcact gcagcccttg gcaacgctgt
     4801 acttgcttgg tatgcttgtg gcatctgttc ttgctgtcga taatttgtgg gtcacagtgt
     4861 actacggagt acctgtatgg aaagaggcgg aaactactct tttttgcgcg tcagacgcaa
     4921 aggcatatga gactgaaaag cataatgtat gggctaccca tgcgtgcgtg cccacagacc
     4981 ccaaccccca agaaatccat ctggagaacg ttactgaaga atttaatatg tggaagaata
     5041 atatggtgga acagatgcat acggacatta tcagtctgtg ggatcagtct ctgaaaccat
     5101 gtgttaaact cacccctctc tgcgtaacct tggactgtaa taacgtgacg aataatggaa
     5161 ctagtgacat gagggaagag ataaagaatt gctccttcaa tatgacaaca gaattgcgag
     5221 acaagcgaca aaaagtatat tctctgttct acaaattgga catagtgcag ataaatgagg
     5281 atcaaggtaa cagctccaat aacaagtatc gcctgataac ttgcaacacg tctgcgatta
     5341 cccaagcatg tcctaaggtt acatttgaac ccattccgat tcattattgc gcacctgcag
     5401 gctttgctat cctgaagtgc aaagatgaag agtttaacgg gatcggcccg tgcaaaaacg
     5461 tatccactgt ccaatgcacc cacggtatta agcctgttgt gtccacacag ctccttctca
     5521 atgggtctct ggcagagaag gaggtcaaaa taagatgtga aaatataacg aacaacgcaa
     5581 aaactatcat tgtccaactg gtgaacccag ttaaaatcaa ttgcacccga cctaataata
     5641 atacgagaaa gagtatccac atcggcccag ggcaggcctt ctacgctaca ggcgatataa
     5701 tcggagacat aagacaggcg cactgcaatg taaatcgtac ggaatggaat aatactctcc
     5761 accaggtagt tgagcagttg cgaaagcatt ttaataaaac gattaacttt gctaactcaa
     5821 ccggagggga cttggaaata acaacacact cattcaattg cgggggcgaa tttttttatt
     5881 gtaacacgac taacctgttt aatagcactt ggaaccatac ggctagtatg aactcaaccg
     5941 aatccaatga cacaatcatc ctgccctgtc gaataaaaca aatcatcaat atgtggcaaa
     6001 gagtgggcca agcaatgtat gcacccccaa taagaggtgt cataaggtgt gaatccaata
     6061 taactgggtt gattcttact agggatggag gaaacacgaa cagcacccga gaaactttcc
     6121 gcccaggcgg gggtgatatg agggataatt ggcgatcaga actttacaag tataaggtag
     6181 ttaagataga acccctcggc gttgctccca caaaagccaa gagaagggtt gtagaacgcg
     6241 aaaagcgggc cgtagggatt ggcgctgtat tcatcggatt tctcggagca gctggatcta
     6301 ctatgggtgc agcttcaata acattgacag ttcaagcccg gcaactcctt tcaggtattg
     6361 tacagcagca aagtaacctc ctgcgagcga ttgaggctca gcagcatctc ctgaagctta
     6421 cggtgtgggg tatcaaacag cttcaagcgc gggtactcgc ggttgagcga taccttaagg
     6481 accaacaatt gcttggaatc tggggctgca gcggtaaact tatatgtaca acatctgtgc
     6541 cgtggaacag ttcctggagt aacaaatcac aaaatgagat ttgggacaat atgacctggt
     6601 tgcagtggga taaagaaata tcaaactata cccagatcat ttacgatctc ttggaggagt
     6661 ctcaaaacca gcaggagaaa aacgaacagg acctcctcgc attggataaa tgggccaatt
     6721 tgtggaactg gtttgacata tctaactggc tttggtatat aaagatattc atcatgattg
     6781 tagggggtct tattggtctg aggatagttt tcgcagtctt gtcagtcatt aaccgagtgc
     6841 ggcagggtta ttctcccttg tcatttcaaa ctcacactcc taatccgagg ggactggata
     6901 ggcctgagcg aatagaggaa gagggcggtg agcaagacaa gaaccggtca atcaggctcg
     6961 tgtctggttt cctggcactg gcgtgggatg atctgaggag tctttgtctt ttctcatacc
     7021 atcggttgcg cgacttcata ctcatcgtcg ccaggaccgt agaacttttg ggacattcct
     7081 ccttgaaggg acttcggctc ggatgggagg gattgaagta cttggggaat ctgctgagtt
     7141 attgggggcg ggaattgcga atctccgcca caaatctctt ggatacgatt gctatagtaa
     7201 tagctggatg gacggataga gtaatcgaaa tagggcagcg cctctgtcga gcatttctca
     7261 atatccccag aagaataagg caaggatttg agcgcgctct gctctagtaa ctcgaggccg
     7321 gcaaggccgg atccagacat gataagatac attgatgagt ttggacaaac cacaactaga
     7381 atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc
     7441 attataagct gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt
     7501 cagggggagg tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg tggtatggct
     7561 gattatgatc cggctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc
     7621 agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc
     7681 agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc catgaggtcg actctaga
//
