LOCUS       sequence-202613-       11766 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral gateway destination vector .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11766)
  AUTHORS   Lu G, Middleton RE, Sun H, Naniong M, Ott CJ, Mitsiades CS, Wong KK,
            Bradner JE, Kaelin WG Jr
  TITLE     The myeloma drug lenalidomide promotes the cereblon-dependent 
            destruction of Ikaros proteins.
  JOURNAL   Science. 2014 Jan 17;343(6168):305-9. doi: 10.1126/science.1244917. 
            Epub 2013 Nov 29.
  PUBMED    24292623
REFERENCE   2  (bases 1 to 11766)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.1244917"; journalName: "Science"; date: 
            "2014-01-17- 17"; volume: "343"; issue: "6168"; pages: "305-9"
COMMENT     Sequence Label: plenti-CAG-gate-FLAG-IRES-GFP
FEATURES             Location/Qualifiers
     source          1..11766
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    129..246
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        344..723
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        726..1001
                     /label=chicken beta-actin promoter
     intron          1002..2010
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1934..1956
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2018..2037
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    2126..2250
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(2275..2280,2281..2298,2299..2305)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             2359..3018
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(2425..2444)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             3360..3665
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     3544..3563
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(3706..3830)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             3850..3873
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    3908..4457
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4052..4069)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4279..4298
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(4460..4462,4463..4465,4466..5179)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4460..4462,4463..4465,4466..5176)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4505..4526)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4766..4785)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5113..5134
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    5255..5843
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5308..5328)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5726..5737)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5846..5862)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5847..5863)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6052..6285
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6357..6478
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6394..6413)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6448..6467
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6518..6653
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6673..6692)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6674..6692)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(6715..7073)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6755..6776)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6967..6986
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        7100..7204
                     /gene="bla"
                     /label=AmpR promoter
     promoter        7206..7563
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        7206..7397
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(7229..7249)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      7414..7549
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7598..8392
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             7598..8392
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7652..7671)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8262..8281
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(8580..8599)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    8624..8671
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      9000..9588
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9489..9508
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        9763..10066
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        10068..10266
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     10221..10241
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             10284..10464
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    10511..10636
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11135..11368
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11553..11597
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca
       61 ttcgattagt gaacggatcg gcactgcgtg cgccaattct gcagacaaat ggcagtattc
      121 atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta
      181 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa
      241 aattttcggg tttattacag ggacagcaga gatccagttt ggttagtacc gggcccgctc
      301 tagccctaga tttaaatcga tgtacgggcc agatatacgc gttgacattg attattgact
      361 agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc
      421 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
      481 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
      541 tgggtggact atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
      601 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
      661 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
      721 atgggtcgag gtgagcccca cgttctgctt cactctcccc atctcccccc cctccccacc
      781 cccaattttg tatttattta ttttttaatt attttgtgca gcgatggggg cggggggggg
      841 gggggcgcgc gccaggcggg gcggggcggg gcgaggggcg gggcggggcg aggcggagag
      901 gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt tccttttatg gcgaggcggc
      961 ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc gggagtcgct gcgttgcctt
     1021 cgccccgtgc cccgctccgc gccgcctcgc gccgcccgcc ccggctctga ctgaccgcgt
     1081 tactcccaca ggtgagcggg cgggacggcc cttctcctcc gggctgtaat tagcgcttgg
     1141 tttaatgacg gctcgtttct tttctgtggc tgcgtgaaag ccttaaaggg ctccgggagg
     1201 gccctttgtg cgggggggag cggctcgggg ggtgcgtgcg tgtgtgtgtg cgtggggagc
     1261 gccgcgtgcg gcccgcgctg cccggcggct gtgagcgctg cgggcgcggc gcggggcttt
     1321 gtgcgctccg cgtgtgcgcg aggggagcgc ggccgggggc ggtgccccgc ggtgcggggg
     1381 ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg tgggggggtg agcagggggt
     1441 gtgggcgcgg cggtcgggct gtaacccccc cctgcacccc cctccccgag ttgctgagca
     1501 cggcccggct tcgggtgcgg ggctccgtgc ggggcgtggc gcggggctcg ccgtgccggg
     1561 cggggggtgg cggcaggtgg gggtgccggg cggggcgggg ccgcctcggg ccggggaggg
     1621 ctcgggggag gggcgcggcg gccccggagc gccggcggct gtcgaggcgc ggcgagccgc
     1681 agccattgcc ttttatggta atcgtgcgag agggcgcagg gacttccttt gtcccaaatc
     1741 tggcggagcc gaaatctggg aggcgccgcc gcaccccctc tagcgggcgc gggcgaagcg
     1801 gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt cgtgcgtcgc cgcgccgccg
     1861 tccccttctc catctccagc ctcggggctg ccgcaggggg acggctgcct tcggggggga
     1921 cggggcaggg cggggttcgg cttctggcgt gtgaccggcg gctctagagc ctctgctaac
     1981 catgttcatg ccttcttctt tttcctacag ctcctgggca acgtgctggt tattgtgctg
     2041 tctcatcatt ttggcaaaga attcccctag ccgcggaatc tagactgccg gatcgaattg
     2101 tcgacgaatt cggatctggc caatcacaag tttgtacaaa aaagctgaac gagaaacgta
     2161 aaatgatata aatatcaata tattaaatta gattttgcat aaaaaacaga ctacataata
     2221 ctgtaaaaca caacatatcc agtcactatg gcggccgcat taggcacccc aggctttaca
     2281 ctttatgctt ccggctcgta taatgtgtgg attttgagtt aggatccgtc gagattttca
     2341 ggagctaagg aagctaaaat ggagaaaaaa atcactggat ataccaccgt tgatatatcc
     2401 caatggcatc gtaaagaaca ttttgaggca tttcagtcag ttgctcaatg tacctataac
     2461 cagaccgttc agctggatat tacggccttt ttaaagaccg taaagaaaaa taagcacaag
     2521 ttttatccgg cctttattca cattcttgcc cgcctgatga atgctcatcc ggaattccgt
     2581 atggcaatga aagacggtga gctggtgata tgggatagtg ttcacccttg ttacaccgtt
     2641 ttccatgagc aaactgaaac gttttcatcg ctctggagtg aataccacga cgatttccgg
     2701 cagtttctac acatatattc gcaagatgtg gcgtgttacg gtgaaaacct ggcctatttc
     2761 cctaaagggt ttattgagaa tatgtttttc gtctcagcca atccctgggt gagtttcacc
     2821 agttttgatt taaacgtggc caatatggac aacttcttcg cccccgtttt caccatgggc
     2881 aaatattata cgcaaggcga caaggtgctg atgccgctgg cgattcaggt tcatcatgcc
     2941 gtttgtgatg gcttccatgt cggcagaatg cttaatgaat tacaacagta ctgcgatgag
     3001 tggcagggcg gggcgtaaac gcgtggatcc ggcttactaa aagccagata acagtatgcg
     3061 tatttgcgcg ctgatttttg cggtataaga atatatactg atatgtatac ccgaagtatg
     3121 tcaaaaagag gtatgctatg aagcagcgta ttacagtgac agttgacagc gacagctatc
     3181 agttgctcaa ggcatatatg atgtcaatat ctccggtctg gtaagcacaa ccatgcagaa
     3241 tgaagcccgt cgtctgcgtg ccgaacgctg gaaagcggaa aatcaggaag ggatggctga
     3301 ggtcgcccgg tttattgaaa tgaacggctc ttttgctgac gagaacaggg gctggtgaaa
     3361 tgcagtttaa ggtttacacc tataaaagag agagccgtta tcgtctgttt gtggatgtac
     3421 agagtgatat tattgacacg cccgggcgac ggatggtgat ccccctggcc agtgcacgtc
     3481 tgctgtcaga taaagtctcc cgtgaacttt acccggtggt gcatatcggg gatgaaagct
     3541 ggcgcatgat gaccaccgat atggccagtg tgccggtctc cgttatcggg gaagaagtgg
     3601 ctgatctcag ccaccgcgaa aatgacatca aaaacgccat taacctgatg ttctggggaa
     3661 tataaatgtc aggctccctt atacacagcc agtctgcagg tcgaccatag tgactggata
     3721 tgttgtgttt tacagtatta tgtagtctgt tttttatgca aaatctaatt taatatattg
     3781 atatttatat cattttacgt ttctcgttca gctttcttgt acaaagtggt gggaggaggt
     3841 ggatctggcg actacaagga cgacgatgac aagtgactcg agcgggatca attccgcccc
     3901 ccccctaacg ttactggccg aagccgcttg gaataaggcc ggtgtgcgtt tgtctatatg
     3961 ttattttcca ccatattgcc gtcttttggc aatgtgaggg cccggaaacc tggccctgtc
     4021 ttcttgacga gcattcctag gggtctttcc cctctcgcca aaggaatgca aggtctgttg
     4081 aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa gacaaacaac gtctgtagcg
     4141 accctttgca ggcagcggaa ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca
     4201 cgtgtataag atacacctgc aaaggcggca caaccccagt gccacgttgt gagttggata
     4261 gttgtggaaa gagtcaaatg gctctcctca agcgtattca acaaggggct gaaggatgcc
     4321 cagaaggtac cccattgtat gggatctgat ctggggcctc ggtgcacatg ctttacatgt
     4381 gtttagtcga ggttaaaaaa acgtctaggc cccccgaacc acggggacgt ggttttcctt
     4441 tgaaaaacac gataatacca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc
     4501 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg
     4561 cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct
     4621 gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg
     4681 ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt
     4741 ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa
     4801 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga
     4861 cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg tctatatcat
     4921 ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga
     4981 cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt
     5041 gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga
     5101 gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat
     5161 ggacgagctg tacaagtaag cggccgcgac tctagagtcg acctgcaggc atgcaagctt
     5221 caggtagccg gctaacgtta tcaagcttat cgataatcaa cctctggatt acaaaatttg
     5281 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc
     5341 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta
     5401 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt
     5461 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca
     5521 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc
     5581 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt
     5641 gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg
     5701 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg
     5761 cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat
     5821 ctccctttgg gccgcctccc cgcatcgata ccgtcgacct cgatcgagac ctagaaaaac
     5881 atggagcaat cacaagtagc aatacagcag ctaccaatgc tgattgtgcc tggctagaag
     5941 cacaagagga ggaggaggtg ggttttccag tcacacctca ggtaccttta agaccaatga
     6001 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc
     6061 taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag
     6121 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     6181 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     6241 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc
     6301 atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact
     6361 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     6421 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     6481 atgtctggct ctagctatcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     6541 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     6601 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctagggac
     6661 gtacccaatt cgccctatag tgagtcgtat tagcatgtga gcaaaaggcc agcataaatc
     6721 aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt
     6781 aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact
     6841 acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg
     6901 gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag
     6961 aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac
     7021 gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtcaggtgg
     7081 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     7141 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     7201 gagtcctgag gcggaaagaa ccagctgtgg aatgtgtgtc agttagggtg tggaaagtcc
     7261 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg
     7321 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     7381 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     7441 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     7501 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
     7561 aaagatcgat caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca
     7621 cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac
     7681 aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt
     7741 tgtcaagacc gacctgtccg gtgccctgaa tgaactgcaa gacgaggcag cgcggctatc
     7801 gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg
     7861 aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc
     7921 tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc
     7981 ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat
     8041 ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc
     8101 cgaactgttc gccaggctca aggcgagcat gcccgacggc gaggatctcg tcgtgaccca
     8161 tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga
     8221 ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat
     8281 tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc
     8341 tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggact
     8401 ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga tttcgattcc
     8461 accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg
     8521 atcctccagc gcggggatct catgctggag ttcttcgccc accctagggg gaggctaact
     8581 gaaacacgga aggagacaat accggaagga acccgcgcta tgacggcaat aaaaagacag
     8641 aataaaacgc acggtgttgg gtcgtttgtt cataaacgcg gggttcggtc ccagggctgg
     8701 cactctgtcg ataccccacc gagaccccat tggggccaat acgcccgcgt ttcttccttt
     8761 tccccacccc accccccaag ttcgggtgaa ggcccagggc tcgcagccaa cgtcggggcg
     8821 gcaggccctg ccatagcctc aggttactca tatatacttt agattgattt aaaacttcat
     8881 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     8941 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     9001 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     9061 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     9121 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc
     9181 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     9241 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     9301 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     9361 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     9421 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     9481 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     9541 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     9601 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     9661 ttatcccctg attctgtgga taaccgtatt accgccatgc attagttatt aatagtaatc
     9721 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
     9781 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
     9841 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
     9901 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
     9961 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
    10021 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
    10081 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
    10141 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
    10201 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
    10261 aagcagcgcg ttttgcctgt actgggtctc tctggttaga ccagatctga gcctgggagc
    10321 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
    10381 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
    10441 agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa
    10501 accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga
    10561 ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga
    10621 tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg
    10681 gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga
    10741 gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat
    10801 actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa
    10861 tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc
    10921 tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgg
    10981 ccgcgctgat cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat
    11041 ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa
    11101 gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg
    11161 gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat
    11221 tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc
    11281 atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg
    11341 aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt
    11401 gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga
    11461 atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact
    11521 ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag
    11581 ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat
    11641 tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta
    11701 tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc
    11761 cgaggg
//
