LOCUS       sequence-202616-       14126 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Heterologous, cobalt-inducible expression of SQE1 and MRN1 from A. 
            thaliana in Synechocystis sp. PCC6803.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14126)
  AUTHORS   Loeschcke A, Dienst D, Wewer V, Hage-Hulsmann J, Dietsch M, 
            Kranz-Finger S, Huren V, Metzger S, Urlacher VB, Gigolashvili T, 
            Kopriva S, Axmann IM, Drepper T, Jaeger KE
  TITLE     The photosynthetic bacteria Rhodobacter capsulatus and Synechocystis
            sp. PCC 6803 as new hosts for cyclic plant triterpene biosynthesis.
  JOURNAL   PLoS One. 2017 Dec 27;12(12):e0189816. doi: 
            10.1371/journal.pone.0189816. eCollection 2017.
  PUBMED    29281679
REFERENCE   2  (bases 1 to 14126)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2017-12-27"; volume: "12(12):e0189816. doi"; pages: " 
            10.1371/journal.pone.0189816. eCollection 2017"
COMMENT     Sequence Label: pVZ_PcoaT_SQE1_MRN1
FEATURES             Location/Qualifiers
     source          1..14126
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             437..1228
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVMHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     terminator      complement(1344..1391)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     1387..1405
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     CDS             complement(1458..1475)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             3789..3800
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     misc_feature    6599..6620
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     CDS             complement(6827..7678)
                     /codon_start=1
                     /gene="repC"
                     /product="replication protein C of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepC
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     CDS             complement(7665..8504)
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepA
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             complement(9015..9986)
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepB
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     oriT            11225..11312
                     /label=RSF1010 oriT
                     /note="origin of transfer of the broad-host-range plasmid 
                     RSF1010 (Scholz et al., 1989)"
     rep_origin      11654..12048
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     primer_bind     complement(12895..12914)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             12906..13721
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             12906..13721
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(12979..12998)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 gcccggatta cagatcctct agaagaacag caaggccgcc aatgcctgac gatgcgtgga
       61 gaccgaaacc ttgcgctcgt tcgccagcca ggacagaaat gcctcgactt cgctgctgcc
      121 caaggttgcc gggtgacgca cactgtggaa acggatgaag gcacgaaccc agtggacata
      181 agcctgttcg gttcgtaagc tgtaatgcaa gtagcgtatg cgctcacgca actggtccag
      241 aaccttgacc gaacgcagcg gtggtaacgg cgcagtggcg gttttcatgg cttgttatga
      301 ctgttttttt ggggtacagt ctatgcctcg ggcatccaag cagcaagcgc gttacgccgt
      361 gggtcgatgt ttgatgttat ggagcagcaa cgatgttacg cagcagggca gtcgccctaa
      421 aacaaagtta aacatcatga gggaagcggt gatcgccgaa gtatcgactc aactatcaga
      481 ggtagttggc gtcatcgagc gccatctcga accgacgttg ctggccgtac atttgtacgg
      541 ctccgcagtg gatggcggcc tgaagccaca cagtgatatt gatttgctgg ttacggtgac
      601 cgtaaggctt gatgaaacaa cgcggcgagc tttgatcaac gaccttttgg aaacttcggc
      661 ttcccctgga gagagcgaga ttctccgcgc tgtagaagtc accattgtta tgcacgacga
      721 catcattccg tggcgttatc cagctaagcg cgaactgcaa tttggagaat ggcagcgcaa
      781 tgacattctt gcaggtatct tcgagccagc cacgatcgac attgatctgg ctatcttgct
      841 gacaaaagca agagaacata gcgttgcctt ggtaggtcca gcggcggagg aactctttga
      901 tccggttcct gaacaggatc tatttgaggc gctaaatgaa accttaacgc tatggaactc
      961 gccgcccgac tgggctggcg atgagcgaaa tgtagtgctt acgttgtccc gcatttggta
     1021 cagcgcagta accggcaaaa tcgcgccgaa ggatgtcgct gccgactggg caatggagcg
     1081 cctgccggcc cagtatcagc ccgtcatact tgaagctaga caggcttatc ttggacaaga
     1141 agaagatcgc ttggcctcgc gcgcagatca gttggaagaa tttgtccact acgtgaaagg
     1201 cgagatcacc aaggtagtcg gcaaataatg tctaacaatt cgttcaagcc gacgccgctt
     1261 cgcggcgcag cttaactcaa gctctagagt cgactgcagc ggccgctact agtcgccaat
     1321 ccggatatag ttcctccttt cagcaaaaaa cccctcaaga cccgtttaga ggccccaagg
     1381 ggttatgcta gttattgctc agcggtggca gcagccaact cagcttcctt tcgggctttg
     1441 ttagcagccg gatctcagtg gtggtggtgg tggtgctcga gtgcggccgc aagcttttag
     1501 ctcaccagca ggcgcagcgc gttggtatac agcgccagcg cccacaggct gaacatgttg
     1561 cgataggtcg gataatgcag cagcatgttc atatagatgc cgcggcgttc ctgctgcggg
     1621 aaatcgccga tatccagctg gctgttgatc agcactttcg ccgcgcgatg caccgggctc
     1681 ggatcgcgtt ccatctggcc gccgatcagc agcaccatca tcgcctggcc ggtgttcacc
     1741 acgttggtca cgttgccttt gctcgggata tatttcttgt tcgggcagct cagcgcgctt
     1801 tcgccccagc cgccttccgc gttctgggtg ttcaggatga actgcaccgc gcggcggatc
     1861 gcttcgctgt tgccgaaggt tttgcccgcc gccgccaggc cgcgcaccgc aaacagggtg
     1921 ccataggtga agcacacgcc ccagttgcca taccagctgc catccggcat ctgcagatct
     1981 tcgatatatt tcaggccttt cttgatcagt ttttcgattt ctttcgggcg atgatccggg
     2041 aattctttct tgaagcacac caggcccgcg atcacgctgc cggtgcattc cagatattcc
     2101 tgttccacca cggtgttttc cacatgttcc accgggctca gccattccag ccatttcttg
     2161 cccggcgccg gttcccacac cggcatgccg ccgttatcgc tctgcagata gatcaggaag
     2221 ttcaccgcat catacagttt ttccacatcc attttttcgc cgatgaattc gctcggcatg
     2281 cgatggaaat ggatgcagca ttcgatgctt tccgcggtgc catcgctgat cggcaggccc
     2341 tgttcgcgat cctggaaggt ccagccgcct ttggtgatat cgcggaacat tttcagatga
     2401 tcgccctgcg ggttttcgct gatctggctc tgtttcagga aatcatagcc tttgatcagg
     2461 gtgctgcgga tttcatcatc catgttcgcc gccagcatca cctgcaggct cagcgccgcg
     2521 ttccagctct gcatgcccat cagctggaat ttcaggccat cttccgcgat ccagatgaaa
     2581 tccggcacgc gcgccagatg tttcttgaaa taatcgccat ccggatcttc cacccagcac
     2641 gccagcatgt ggaacggttt cgccacgcac gcgccggtga tataatgggt ggtttcatca
     2701 tgataatgca ccagttccat ggtggtgcgc agcgcgcgct ggcgcacgaa tttgttcagc
     2761 ggccagcggt tgatgatgtt ttcgctcagc atgtgcacgc cttcccagaa cagatcctgg
     2821 ccgaagctct tcggatacag cagatcttct ttcgcgcagc ggttgcgcgc tttgctccag
     2881 atgattttgc tatacggctg cggatacagt tcttcgcgca gctgcaggat cagcgcggtc
     2941 ggggtcgcca cgaatttctt gccatacaga tagctcatgc ccatcagcag atcgcggata
     3001 tagatccaca gggtgccgcc gttgatcggg ctgaagctcg gcagcatcca gatttccggc
     3061 ggcagcggtt tgcagccgct ccaatcatac acgcccagca cgctcagcca cgctttcgcc
     3121 accatcggcg cataggtcgc gccgccatga tccaggatcc atttgcgcgc gcgcgcgcac
     3181 gcgcttttct ggccatcatg atccggttcc acgcccagga tgcgcaggca gatatagttc
     3241 agcacggtgc agaacatcac gctatggctt tccacatcca ggccccagcc gccatcttcg
     3301 ttctgggtgt tatacatata gcgcagcagt tctttcacat gttccagggt gaagattttt
     3361 tccagatggc cggtgatata caggcagatc acgaacggcg catccaggaa catgctgccg
     3421 ctcacttcgc tcggccaatg gccatcgttc gcctggcacg ccgcataata cagcacgccg
     3481 cggcgcagcg cggttttcgc atcttcatag gtgatttttt ccgcatcttc gatgcgcacg
     3541 cgcgggattt tctgttcgaa ttttttttcg cgcaggaact ggcggcgcca gaacagatcc
     3601 gggctcgctt tgaagcggct tttgttgttc gcgaagttca ggcgcgcttc ttccacttcc
     3661 gccagttctt ccgggctgcc gccgttcgca tcgaattccc agatctggcg gcccgcgaag
     3721 ttgttggtgg tgaacagatg cggatcctgg cgcgcttccg cgccgatgcg caggcgccac
     3781 atctagtatt tctcctcttt tacgcgttta gctgcatttg gtttcgccca cggtcggcgc
     3841 tttataataa tacgccggca cggtcgccgg gaagaacatc tggcgcacgc cttccgcttt
     3901 gatgatcggg aagatgatgc cgctcgcgcc gctgatcagt ttcgcgccca gccagatgcg
     3961 tttcgggctc gggaacggga tcagcaggcg gatcacgcca tacaccgcca ccgcgaagaa
     4021 atggcacacc agggtcagcg ggcgcgggtt caggccgctc agcaggctca ccgggccgct
     4081 ggtgcacatg ccgcccaggc ccagataatc gaagcacgct tcgcgcattt cgttgcgcgc
     4141 ttcgttttcg ctgctgcaga acacctgata cagcgcgttc gccagggtgt tgatggtcgc
     4201 cgccaccggt ttgcgcaggg tatagaagct ctccagatat ttgcacaggc tcgcgccatc
     4261 gctcagatcg cgcagcgggc gcagcaggtt gcgcagcacc acgatatccg ccagcgccac
     4321 ggtcatgccg ccgccggtca gcggatggcg catgttgaac gcatcgccca tcagcagcgc
     4381 gcccggggtc ggatacgggc tcgccggcat gctgcggttc ggcatgcttt tgatgttgcc
     4441 tttatccacc gccgcgatga agctatcata cacttcatgc ggcatctgcg gcgccaccac
     4501 ggttttcaga tagtttttca tttcgccgtt cgcgatgctc ggcactttct ggcccggcac
     4561 atccaccagg cagcgcactt cggtgctgct gatcggatac atcaggatcg ggctcggatc
     4621 cgccagcacc acatggccat ggttcgcata cggcaggttg cagttttcca gcaccaggcc
     4681 cacgaagcag ctcggcactt ccacctgcgg gttgcacagg ctgcggcgca ggttgctgaa
     4741 gcagccatcg cacacgatgg tcagcgccgc gaacgcggtc tgttcttcgc ccgctttgtt
     4801 tttatagcgc acgcctttga tggtgccgtt ttcttccagc aggctcagca cggtgccctg
     4861 ttccagctgc acgttcggca ggctcgccgc tttttcgcgc atgcgctgga tgaagcggcc
     4921 gttatggaag ctgcggccgc tcacatcttc atggaatttt tccagcggat acgccaggcg
     4981 gatgcgtttg ccgtttttga acagcgcata gccatacacg cgctgcgcat cgatttcttc
     5041 cacgcaatct tcgatgccca gttccagcag tttcagatag ccgcccggct gcagcagttc
     5101 gcccacgatg cgatccggtt cgctcagatc gcgttcgatc acatgcacgc ggcgtttatc
     5161 tttgcccagg gtatacgcca gcgcgctgcc cgccacgccc gcgcccacca cgatcacatc
     5221 cgccacggta tcgccggtga tgttcacgct gcccacatcg ctggtcacgg tgctcacggt
     5281 tttgcgatca tggcgcaggc cgttgcgttt cggtttcacg aagaagccat agaacgccac
     5341 gaagctgatc agcaggctgc tgatcagcag cggcaggatc cagttccaca gctggctagc
     5401 catgcttttt aacttggatt tttaccttct cagcctaaac cttaacatta gtgtcaatgt
     5461 caaggttttg agtaaactaa agggatgaag actaatcact taacgattaa agaactcaca
     5521 gatgcagtgg gaggtggcgt tacgcctcgc atggtgcgcc attaccacac cctgggattg
     5581 cttccccccg ttcaacgctc agagggcaac taccgccttt atactcagca ggacgtacaa
     5641 cggctccaac gagtcattgc cctcaaacag cagggctttc agttgtctca tattcggcaa
     5701 ctgctggata gccattctga agagagcctt gatcccaccc taatggtgca gttgcaacag
     5761 caatatcagg ctgtgattca gcagattact cgactccgcc aaaccgcatc tgctttagaa
     5821 ggattactgg gacgcgacca gagttgccag attacccaag cggaagctct ggcccaactg
     5881 aaacagcttg atgtggatgt ccaggagggg ttaggaaaac tcgatcagtt gtggacgaac
     5941 ctagatgcgg agacaacaac tcatccagaa gcctttcagg aatccctcaa acacctgcta
     6001 ccggatttat cggcttactc tgaaattacc attcacttac tacatcaatt agtgctggcc
     6061 tgtggtgatg ttagcttggt aaacgccgtt cgattgagtc agggagcgat cgcctcggca
     6121 cgagatgcac tgaaagcagg gtgtccagtc gtcaccgatg ttccggttgt ggctgcggct
     6181 cttgatcaaa ctcggttagc tcatttagga tgtacggtta aaacgctgat tgacgaccct
     6241 cacatcacag ggcttaggga agccgagcaa gctttttggc accatgacca ttggcaacag
     6301 cggttacaac agattcccca aggatgtgtg ctggcgatcg gctatgcccc ttctgtttta
     6361 ctcactgcct gtaagctgat agagcaacaa catattcagc cggctcttgt gatcggaatg
     6421 ccgatcggtt ttagtcatgc tccgggggca aaacgacgac tgatgaccag tcccattccc
     6481 catatcacca ttcaggggag cctcggtgga ggacttctag ccgcagtaac gctaaacgct
     6541 ttagtggaaa cattgattgc aaagccagat tgccactgct atctcacttg tctttagtct
     6601 ctagaagcgg ccgcgaattc atgcaggagc agaagagcat acatctggaa gcaaagccag
     6661 gaaagcggcc tatggagctg tgcggcagcg ctcagtaggc aatttttcaa aatattgtta
     6721 agccttttct gagcatggta tttttcatgg tattaccaat tagcaggaaa ataagccatt
     6781 gaatataaaa gataaaaatg tcttgtttac aatagagtgg ggggggtcag cctgccgcct
     6841 tgggccgggt gatgtcgtac ttgcccgccg cgaactcggt taccgtccag cccagcgcga
     6901 ccagctccgg caacgcctcg cgcacccgct ggcggcgctt gcgcatggtc gaaccactgg
     6961 cctctgacgg ccagacatag ccgcacaagg tatctatgga agccttgccg gttttgccgg
     7021 ggtcgatcca gccacacagc cgctggtgca gcaggcgggc ggtttcgctg tccagcgccc
     7081 gcacctcgtc catgctgatg cgcacatgct ggccgccacc catgacggcc tgcgcgatca
     7141 aggggttcag ggccacgtac aggcgcccgt ccgcctcgtc gctggcgtac tccgacagca
     7201 gccgaaaccc ctgccgcttg cggccattct gggcgatgat ggataccttc caaaggcgct
     7261 cgatgcagtc ctgtatgtgc ttgagcgccc caccactatc gacctctgcc ccgatttcct
     7321 ttgccagcgc ccgatagcta cctttgacca ccatggcatc agcggtgacg gcctcccact
     7381 tgggttccag gaacagccgg agctgccgtc cgccttcggt cttgggttcc gggccaagca
     7441 ctaggccatt aggcccagcc atggccacca gcccttgcag gatgcgcaga tcatcagcgc
     7501 ccagcggctc cgggccgctg aactcgatcc gcttgccgtc gccgtagtca tacgtcacgt
     7561 ccagcttgct gcgcttgcgc tcgccccgct tgagggcacg gaacaggccg ggggccagac
     7621 agtgcgccgg gtcgtgccgg acgtggctga ggctgtgctt gttcttaggc ttcaccacgg
     7681 ggcaccccct tgctcttgcg ctgcctctcc agcacggcgg gcttgagcac cccgccgtca
     7741 tgccgcctga accaccgatc agcgaacggt gcgccatagt tggccttgct cacaccgaag
     7801 cggacgaaga accggcgctg gtcgtcgtcc acaccccatt cctcggcctc ggcgctggtc
     7861 atgctcgaca ggtaggactg ccagcggatg ttatcgacca gtaccgagct gccccggctg
     7921 gcctgctgct ggtcgcctgc gcccatcatg gccgcgccct tgctggcatg gtgcaggaac
     7981 acgatagagc acccggtatc ggcggcgatg gcctccatgc gaccgatgac ctgggccatg
     8041 gggccgctgg cgttttcttc ctcgatgtgg aaccggcgca gcgtgtccag caccatcagg
     8101 cggcggccct cggcggcgcg cttgaggccg tcgaaccact ccggggccat gatgttgggc
     8161 aggctgccga tcagcggctg gatcagcagg ccgtcagcca cggcttgccg ttcctcggcg
     8221 ctgaggtgcg ccccaagggc gtgcaggcgg tgatgaatgg cggtgggcgg gtcttcggcg
     8281 ggcaggtaga tcaccgggcc ggtgggcagt tcgcccacct ccagcagatc cggcccgcct
     8341 gcaatctgtg cggccagttg cagggccagc atggatttac cggcaccacc gggcgacacc
     8401 agcgccccga ccgtaccggc caccatgttg ggcaaaacgt agtccagcgg tggcggcgct
     8461 gctgcgaacg cctccagaat attgataggc ttatgggtag ccattgattg cctcctttgc
     8521 aggcagttgg tggttaggcg ctggcggggt cactaccccc gccctgcgcc gctctgagtt
     8581 cttccaggca ctcgcgcagc gcctcgtatt cgtcgtcggt cagccagaac ttgcgctgac
     8641 gcatcccttt ggccttcatg cgctcggcat atcgcgcttg gcgtacagcg tcagggctgg
     8701 ccagcaggtc gccggtctgc ttgtcctttt ggtctttcat atcagtcacc gagaaacttg
     8761 ccggggccga aaggcttgtc ttcgcggaac aaggacaagg tgcagccgtc aaggttaagg
     8821 ctggccatat cagcgactga aaagcggcca gcctcggcct tgtttgacgt ataaccaaag
     8881 ccaccgggca accaatagcc cttgtcactt ttgatcaggt agaccgaccc tgaagcgctt
     8941 ttttcgtatt ccataaaacc cccttctgtg cgtgagtact catagtataa caggcgtgag
     9001 taccaacgca agcactacat gctgaaatct ggcccgcccc tgtccatgcc tcgctggcgg
     9061 ggtgccggtg cccgtgccag ctcggcccgc gcaagctgga cgctgggcag acccatgacc
     9121 ttgctgacgg tgcgctcgat gtaatccgct tcgtggccgg gcttgcgctc tgccagcgct
     9181 gggctggcct cggccatggc cttgccgatt tcctcggcac tgcggccccg gctggccagc
     9241 ttctgcgcgg cgataaagtc gcacttgctg aggtcatcac cgaagcgctt gaccagcccg
     9301 gccatctcgc tgcggtactc gtccagcgcc gtgcgccggt ggcggctaag ctgccgctcg
     9361 ggcagttcga ggctggccag cctgcgggcc ttctcctgct gccgctgggc ctgctcgatc
     9421 tgctggccag cctgctgcac cagcgccggg ccagcggtgg cggtcttgcc cttggattca
     9481 cgcagcagca cccacggctg ataaccggcg cgggtggtgt gcttgtcctt gcggttggtg
     9541 aagcccgcca agcggccata gtggcggctg tcggcgctgg ccgggtcggc gtcgtactcg
     9601 ctggccagcg tccgggcaat ctgcccccga agttcaccgc ctgcggcgtc ggccaccttg
     9661 acccatgcct gatagttctt cgggctggtt tccactacca gggcaggctc ccggccctcg
     9721 gctttcatgt catccaggtc aaactcgctg aggtcgtcca ccagcaccag accatgccgc
     9781 tcctgctcgg cgggcctgat atacacgtca ttgccctggg cattcatccg cttgagccat
     9841 ggcgtgttct ggagcacttc ggcggctgac cattcccggt tcatcatctg gccggtggtg
     9901 gcgtccctga cgccgatatc gaagcgctca cagcccatgg ccttgagctg tcggcctatg
     9961 gcctgcaaag tcctgtcgtt cttcatcggg ccaccaagcg cagccagatc gagccgtcct
    10021 cggttgtcag tggcgtcagg tcgagcaaga gcaacgatgc gatcagcagc accaccgtag
    10081 gcatcatgga agccagcatc acggttagcc atagcttcca gtgccacccc cgcgacgcgc
    10141 tccgggcgct ctgcgcggcg ctgctcacct cggcggctac ctcccgcaac tctttggcca
    10201 gctccaccca tgccgcccct gtctggcgct gggctttcag ccactccgcc gcctgcgcct
    10261 cgctggcctg ctgggtctgg ctcatgacct gccgggcttc gtcggccagt gtcgccatgc
    10321 tctgggccag cggttcgatc tgctccgcta actcgttgat gcctctggat ttcttcactc
    10381 tgtcgattgc gttcatggtc tattgcctcc cggtattcct gtaagtcgat gatctgggcg
    10441 ttggcggtgt cgatgttcag ggccacgtct gcccggtcgg tgcggatgcc ccggccttcc
    10501 atctccacca cgttcggccc caggtgaaca ccgggcaggc gctcgatgcc ctgcgcctca
    10561 agtgttctgt ggtcaatgcg ggcgtcgtgg ccagcccgct ctaatgcccg gttggcatgg
    10621 tcggcccatg cctcgcgggt ctgctcaagc catgccttgg gcttgagcgc ttcggtcttc
    10681 tgtgccccgc ccttctccgg ggtcttgccg ttgtaccgct tgaaccactg agcggcgggc
    10741 cgctcgatgc cgtcattgat ccgctcggag atcatcaggt ggcagtgcgg gttctcgccg
    10801 ccaccggcat ggatggccag cgtatacggc aggcgctcgg caccggtcag gtgctgggcg
    10861 aactcggacg ccagcgcctt ctgctggtcg agggtcagct cgaccggcag ggcaaattcg
    10921 acctccttga acagccgccc attggcgcgt tcatacaggt cggcagcatc ccagtagtcg
    10981 gcgggccgct cgacgaactc cggcatgtgc ccggattcgg cgtgcaagac ttcatccatg
    11041 tcgcgggcat acttgccttc gcgctggatg tagtcggcct tggccctggc cgattggccg
    11101 cccgacctgc tgccggtttt cgccgtaagg tgataaatcg ccatgctgcc tcgctgttgc
    11161 ttttgctttt cggctccatg caatggccct cggagagcgc accgcccgaa gggtggccgt
    11221 taggccagtt tctcgaagag aaaccggtaa gtgcgccctc ccctacaaag tagggtcggg
    11281 attgccgccg ctgtgcctcc atgatagcct acgagacagc acattaacaa tggggtgtca
    11341 agatggttaa ggggagcaac aaggcggcgg atcggctggc caagctcgaa gaacaacgag
    11401 cgcgaatcaa tgccgaaatt cagcgggtgc gggcaaggga acagcagcaa gagcgcaaga
    11461 acgaaacaag gcgcaaggtg ctggtggggg ccatgatttt ggccaaggtg aacagcagcg
    11521 agtggccgga ggatcggctc atggcggcaa tggatgcgta ccttgaacgc gaccacgacc
    11581 gcgccttgtt cggtctgccg ccacgccaga aggatgagcc gggctgaatg atcgaccgag
    11641 acaggccctg cggggctgca cacgcgcccc cacccttcgg gtagggggaa aggccgctaa
    11701 agcggctaaa agcgctccag cgtatttctg cggggtttgg tgtggggttt agcgggcttt
    11761 gcccgccttt ccccctgccg cgcagcggtg gggcggtgtg tagcctagcg cagcgaatag
    11821 accagctatc cggcctctgg ccgggcatat tgggcaaggg cagcagcgcc ccacaagggc
    11881 gctgataacc gcgcctagtg gattattctt agataatcat ggatggattt ttccaacacc
    11941 ccgccagccc ccgcccctgc tgggtttgca ggtttggggg cgtgacagtt attgcagggg
    12001 ttcgtgacag ttattgcagg ggggcgtgac agttattgca ggggttcgtg acagttagta
    12061 cgggagtgac gggcactggc tggcaatgtc tagcaacggc aggcatttcg gctgagggta
    12121 aaagaacttt ccgctaagcg atagactgta tgtaaacaca gtattgcaag gacgcggaac
    12181 atgcctcatg tggcggccag gacggccagc cgggatcggg atactggtcg ttaccagagc
    12241 caccgacccg agcaaaccct tctctatcag atcgttgacg agtattaccc ggcattcgct
    12301 gcgcttatgg cagagcaggg aaaggaattg ccgggctatg tgcaacggga atttgaagaa
    12361 tttctccaat gcgggcggct ggagcatggc tttctacggg ttcgctgcga gtcttgccac
    12421 gccgagcacc tggtcgcttt cagctgtaat ccgggcagcg caacggaaca ttcatcagtg
    12481 taaaaatgga atcaataaag ccctgcgcag cgcgcagggt cagcctgaat acgcgtttaa
    12541 tgaccagcac agtcgtgatg gcaaggtcag aatagcgctg aggtctgcct cgtgaagaag
    12601 gtgttgctga ctcataccag gcctgaatcg ccccatcatc cagccagaaa gtgagggagc
    12661 cacggttgat gagagctttg ttgtaggtgg accagttggt gattttgaac ttttgctttg
    12721 ccacggaacg gtctgcgttg tcgggaagat gcgtgatctg atccttcaac tcagcaaaag
    12781 ttcgatttat tcaacaaagc cacgttgtgt ctcaaaatct ctgatgttac attgcacaag
    12841 ataaaaatat atcatcatga acaataaaac tgtctgctta cataaacagt aatacaaggg
    12901 gtgttatgag ccatattcaa cgggaaacgt cttgctcgag gccgcgatta aattccaaca
    12961 tggatgctga tttatatggg tataaatggg ctcgcgataa tgtcgggcaa tcaggtgcga
    13021 caatctatcg attgtatggg aagcccgatg cgccagagtt gtttctgaaa catggcaaag
    13081 gtagcgttgc caatgatgtt acagatgaga tggtcagact aaactggctg acggaattta
    13141 tgcctcttcc gaccatcaag cattttatcc gtactcctga tgatgcatgg ttactcacca
    13201 ctgcgatccc cgggaaaaca gcattccagg tattagaaga atatcctgat tcaggtgaaa
    13261 atattgttga tgcgctggca gtgttcctgc gccggttgca ttcgattcct gtttgtaatt
    13321 gtccttttaa cagcgatcgc gtatttcgtc tcgctcaggc gcaatcacga atgaataacg
    13381 gtttggttga tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt gaacaagtct
    13441 ggaaagaaat gcataagctt ttgccattct caccggattc agtcgtcact catggtgatt
    13501 tctcacttga taaccttatt tttgacgagg ggaaattaat aggttgtatt gatgttggac
    13561 gagtcggaat cgcagaccga taccaggatc ttgccatcct atggaactgc ctcggtgagt
    13621 tttctccttc attacagaaa cggctttttc aaaaatatgg tattgataat cctgatatga
    13681 ataaattgca gtttcatttg atgctcgatg agtttttcta atcagaattg gttaattggt
    13741 tgtaacactg gcagagcatt acgctgactt gacgggacgg cggctttgtt gaataaatcg
    13801 aacttttgct gagttgaagg atcagatcac gcatcttccc gacaacgcag accgttccgt
    13861 ggcaaagcaa aagttcaaaa tcaccaactg gtccacctac aacaaagctc tcatcaaccg
    13921 tggctccctc actttctggc tggatgatgg ggcgattcag gcctggtatg agtcagcaac
    13981 accttcttca cgaggcagac ctcagcgcta ttctgacctt gccatcacga ctgtgctggt
    14041 cattaaacgc gtattcaggc tgaccctgcg cgctgcgcag ggctttattg attccatttt
    14101 tacactgatg aatgttccgt tgcgct
//
