LOCUS       sequence-202627-        1878 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian cell expression plasmid for potassium ion ratiometric 
            indicator KIRIN1 with nuclear exclusion sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1878)
  AUTHORS   Shen Y, Wu SY, Rancic V, Aggarwal A, Qian Y, Miyashita SI, Ballanyi 
            K, Campbell RE, Dong M
  TITLE     Genetically encoded fluorescent indicators for imaging intracellular
            potassium ion concentration.
  JOURNAL   Commun Biol. 2019 Jan 14;2:18. doi: 10.1038/s42003-018-0269-2. 
            eCollection 2019.
  PUBMED    30652129
REFERENCE   2  (bases 1 to 1878)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2019-01-14"; volume: "2:18. doi"; pages: " 
            10.1038/s42003-018-0269-2. eCollection 2019"
COMMENT     Sequence Label: pcDNA-NES-KIRIN1
FEATURES             Location/Qualifiers
     source          1..1878
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(46..67)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(307..326)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
       61 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac
      121 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
      181 ctcgtgacca ccctgagctg gggcgtgcag tgcttcgccc gctaccccga ccacatgaag
      241 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
      301 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
      361 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
      421 aagctggagt acaacgccat ccacggcaac gtctatatca ccgccgacaa gcagaagaac
      481 ggcatcaagg ccaacttcgg cctcaactgc aacatcgagg acggcagcgt gcagctcgcc
      541 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
      601 tacctgagca cccagtccaa gctgagcaaa gaccccaacg agaagcgcga tcacatggtc
      661 ctgctggagt tcgtgaccgc ggccgcaggc ctgttcaact tcgtgaagga cgccggcgag
      721 aagctgtggg atgccgtgac aggccagcac gacaaggacg accaggccaa gaaggtgcag
      781 gaacacctga acaagaccgg catccccgac gccgacaaag tgaacatcca gatcgccgac
      841 ggcaaggcca cagtgacagg cgacggactg agccaggaag ccaaagaaaa gatcctggtg
      901 gccgtgggca acatcagcgg aatcgccagc gtggacgatc aagtgaaaac cgccacccct
      961 gccaccgcca gccagttcta cacagtgaag tccggcgaca ccctgagcgc catcagcaaa
     1021 caggtgtacg gcaacgccaa cctgtacaac aagattttcg aggccaacaa gcccatgctg
     1081 aagtcccccg acaaaatcta ccccggccag gtgctgagaa tccccgagga actggagctc
     1141 atgggtgggg tccagcttgc agatcactac cagcagaata ctccgatcgg ggatgggccg
     1201 gttctcctgc ctgacaacca ctacctgtca taccagtcca agctgtccaa agatcctaat
     1261 gaaaagcggg atcacatggt cctgctggaa tttgtcaccg ccgccggaat cactctcgga
     1321 atggacgagc tgtacaaagg tgggaccggg ggatctatgg tgagcaaggg cgaagaattg
     1381 ttcactggtg tagtccctat tttggtggaa ctggatgggg atgtcaacgg tcataaattc
     1441 agcgtgcgag gggagggtga gggggacgca actaatggca aattgacact caagctcatc
     1501 tgcacaacgg ggaaactgcc cgtgccgtgg cccacactcg ttactactct aggatatggc
     1561 cttatgtgtt tcgcccgcta cccagaccac atgaaacaac acgatttctt taaatcagcc
     1621 atgccagagg ggtacgtcca ggagagaact atcttcttca aggacgatgg taattacaag
     1681 actagggccg aggtgaaatt cgaaggggat acacttgtca accgaatcga gctgaaggga
     1741 atcgacttta aggaggacgg aaacatactc gggcataagt tggaatacaa ctacaacagt
     1801 cacaatgttt acatcacagc cgataagcaa aagaacggca ttaaggctaa ttttaagatc
     1861 cgtcacaaca ttgaggat
//
