LOCUS       sequence-202633-        7641 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Calcium channel beta2a auxillary subunit in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7641)
  AUTHORS   Wyatt CN, Page KM, Berrow NS, Brice NL, Dolphin AC
  TITLE     The effect of overexpression of auxiliary Ca2+ channel subunits on 
            native Ca2+ channel currents in undifferentiated mammalian NG108-15 
            cells.
  JOURNAL   J Physiol. 1998 Jul 15;510 ( Pt 2):347-60.
  PUBMED    9705988
REFERENCE   2  (bases 1 to 7641)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J 
            Physiol."; date: "1998-07-15"; volume: "510 ( Pt 2)"; pages: 
            "347-60"
COMMENT     Sequence Label: cacnb2a (rat) in pMT2 vector
FEATURES             Location/Qualifiers
     source          1..7641
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(7..336)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      21..156
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     321..341
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     intron          805..1034
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     1017..1036
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             3689..4252
                     /codon_start=1
                     /gene="Mus musculus Dhfr"
                     /product="mouse dihydrofolate reductase"
                     /label=DHFR
                     /translation="MVRPLNCIVAVSQNMGIGKNGDLPWPPLRNEFKYFQRMTTTSSVE
                     GKQNLVIMGRKTWFSIPEKNRPLKDRINIVLSRELKEPPRGAHFLAKSLDDALRLIEQP
                     ELASKVDMVWIVGGSSVYQEAMNQPGHLRLFVTRIMQEFESDTFFPEIDLGKYKLLPEY
                     PGVLSEVQEEKGIKYKFEVYEKKD"
     polyA_signal    4440..4574
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4477..4496)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4531..4550
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5247..5264)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5418..6006)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5498..5517)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6177..6968,6969..7037))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6800..6819
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7038..7142)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7210..7228
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(7266..7288)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     7388..7407
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7601..7623
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7615..7632
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7616..7632
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 aagctttttg caaaagccta ggcctccaaa aaagcctcct cactacttct ggaatagctc
       61 agaggccgag gcggcctcgg cctctgcata aataaaaaaa attagtcagc catggggcgg
      121 agaatgggcg gaactgggcg gagttagggg cgggatgggc ggagttaggg gcgggactat
      181 ggttgctgac taattgagat gcatgctttg catacttctg cctgctgggg agcctgggga
      241 ctttccacac ctggttgctg actaattgag atgcatgctt tgcatacttc tgcctgctgg
      301 ggagcctggg gactttccac accctaactg acacacattc cacaggatcc ggtcgcgcga
      361 atttcgagcg gtgttccgcg gtcctcctcg tatagaaact cggaccactc tgagacgaag
      421 gctcgcgtcc aggccagcac gaaggaggct aagtgggagg ggtagcggtc gttgtccact
      481 agggggtcca ctcgctccag ggtgtgaaga cacatgtcgc cctcttcggc atcaaggaag
      541 gtgattggtt tataggtgta ggccacgtga ccgggtgttc ctgaaggggg gctataaaag
      601 ggggtggggg cgcgttcgtc ctcactctct tccgcatcgc tgtctgcgag ggccagctgt
      661 tgggctcgcg gttgaggaca aactcttcgc ggtctttcca gtactcttgg atcggaaacc
      721 cgtcggcctc cgaacggtac tccgccaccg agggacctga gcgagtccgc atcgaccgga
      781 tcggaaaacc tctcgactgt tggggtgagt actccctctc aaaagcgggc atgacttctg
      841 cgctaagatt gtcagtttcc aaaaacgagg aggatttgat attcacctgg cccgcggtga
      901 tgcctttgag ggtggccgcg tccatctggt cagaaaagac aatctttttg ttgtcaagct
      961 tgaggtgtgg caggcttgag atctggccat acacttgagt gacaatgaca tccactttgc
     1021 ctttctctcc acaggtgtcc actcccaggt ccaactgcag gcgagcctga attccggttt
     1081 ttgtaactta cttgttttag tatatactga tctgaacagg caaaacccca tgttagcaac
     1141 agctcggtca ggaagagaaa tgctaccttt tctagatgtt tgccgggagg cagccccctg
     1201 aaaggagatc tcaaattacc tgtaagaaaa aaaatttttt taaaggcagt cagagagagc
     1261 aaagttttgg catgcctgca aaaacagcag tgtctgtaaa aactacctag agacagcagc
     1321 taagtgttga tttgcccatg actcttacct gggagtggaa ggtgggaaga aatggacagt
     1381 ggccgtgacg agaagctgag gcacggtgca gcttggtgaa gccacgctct gactgcgtcc
     1441 tgccacctct tcatgcagtg ctgcgggctg gtacatcgcc ggcgagtacg ggtgtcctat
     1501 ggttcggcag actcctacac cagccgccca tccgattcag atgtgtcttt ggaagaggac
     1561 cgggaggcag tccgtagaga agctgagcgg caggcccagg cccagttgga aaaagcaaag
     1621 acaaaacctg ttgcatttgc agttcggacc aatgtcaggt acagcgcagc ccaggaggat
     1681 gacgtcccgg tgcccggcat ggccatctca ttcgaggcaa aagattttct gcatgttaag
     1741 gaaaaattta ataatgactg gtggatagga cggctggtta aagaaggctg tgaaatcgga
     1801 tttattccaa gcccggtcaa actagagaac atgaggctac agcatgaaca gagagccaag
     1861 caagggaaat tctactccag taaatcagga ggaaattcat catccagttt gggtgacata
     1921 gtacccagtt ccagaaaatc gacacctcca tcatctgcta tagacataga tgctactggc
     1981 ttagatgcag aagagaatga tattccagca aaccaccgct cccctaagcc cagtgcaaac
     2041 agtgtaacgt caccccactc caaagagaaa agaatgccct tctttaagaa gacagagcac
     2101 actcctcctt acgatgtggt accatccatg agaccagtgg tgttggtggg cccctccctg
     2161 aaggggtatg aggtcacaga tatgatgcaa aaagcactgt ttgattttct aaaacacaga
     2221 tttgaaggac ggatatccat cacaagagtc actgctgaca tctccctggc caaacgctca
     2281 gtattaaaca accccagtaa gcacgcaata atagaaagat ccaacacaag gtcgagctta
     2341 gcggaagttc agagtgaaat tgaaaggatt tttgaacttg caagaacact gcaattggta
     2401 gtccttgacg cggatacaat taatcaccca gctcaactca gtaagacctc tctggcccct
     2461 atcatagtgt atgtaaagat ttcttctcct aaggttttac aaaggttaat aaaatctcga
     2521 ggaaagtctc aagcaaagca cctcaatgtc cagatggtgg cagccgataa actggcccag
     2581 tgtcccccgc aggagtcatt tgatgtgatc ttggatgaga accaacttga ggatgcttgt
     2641 gagcatctcg ccgactatct ggaggcgtac tggaaggcca cccaccctcc cagcagtaac
     2701 ctccccaacc ctctccttag ccggacttta gccacctcaa ctttacctct gagccccacc
     2761 cttgcctcta attcacaggg ttctcaaggt gatcagagga ctgatcgctc tgctccccgg
     2821 tctgcttccc aagctgaaga agaaccttgt ctggaaccag tcaaaaaatc ccaacaccgt
     2881 tcttcctcag ccacacacca aaaccaccgc agtgggacag gtcgaggcct ctctaggcaa
     2941 gaaacgtttg actctgaaac ccaagagagc cgagactctg cctacgtgga gccaaaggaa
     3001 gattattcac atgaacatgt ggaccgctat gttccacacc gtgaacataa ccacagagag
     3061 gagagccaca gcagcaatgg ccacaggcac agggagcctc gccaccgcac tagggacatg
     3121 ggtcgagacc aagaccacaa tgagtgcagc aaacaacgaa gccggcataa atctaaggat
     3181 cgctactgtg acaaggaagg ggaagtaata tccaaaagaa ggagtgaggc tggcgagtgg
     3241 aacagggatg tatacatccg ccaatgaccg tgcgtgtctc tgcccccaag tcttgtgtaa
     3301 catcatcctt aagcaaaatc tttggggctc cattgcaacc atctgtgatc tgtcttgtat
     3361 attttgtatt gctgttgctt gaatagcaat agcatgcaat caagtattac tatacattct
     3421 tttgtaagta ccacagaaat tggcctagta caagctgcag tcctcagtct atatcctgaa
     3481 ctcttcaaaa gctgtttggg gtagctgcca cctgaacaga gtggcttcag aaatatagtt
     3541 aatgtattca gtgagccaga tgcagcacag ataacaagtg gaattccggg gggggggggg
     3601 ggggggacag ctcagggctg cgatttcgcg ccaaacttga cggcaatcct agcgtgaagg
     3661 ctggtaggat tttatccccg ctgccatcat ggttcgacca ttgaactgca tcgtcgccgt
     3721 gtcccaaaat atggggattg gcaagaacgg agacctaccc tggcctccgc tcaggaacga
     3781 gttcaagtac ttccaaagaa tgaccacaac ctcttcagtg gaaggtaaac agaatctggt
     3841 gattatgggt aggaaaacct ggttctccat tcctgagaag aatcgacctt taaaggacag
     3901 aattaatata gttctcagta gagaactcaa agaaccacca cgaggagctc attttcttgc
     3961 caaaagtttg gatgatgcct taagacttat tgaacaaccg gaattggcaa gtaaagtaga
     4021 catggtttgg atagtcggag gcagttctgt ttaccaggaa gccatgaatc aaccaggcca
     4081 cctcagactc tttgtgacaa ggatcatgca ggaatttgaa agtgacacgt ttttcccaga
     4141 aattgatttg gggaaatata aacttctccc agaataccca ggcgtcctct ctgaggtcca
     4201 ggaggaaaaa ggcatcaagt ataagtttga agtctacgag aagaaagact aacaggaaga
     4261 tgctttcaag ttctctgctc ccctcctaaa gctatgcatt ttttataaga ccatgggact
     4321 tttgctggct ttagatcata atcagccata ccacatttgt agaggtttta cttgctttaa
     4381 aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta
     4441 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     4501 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     4561 atcatgtctg gatccccggc caacggtctg gtgacccggc tgcgagagct cggtgtacct
     4621 gagacgcgag taagcccttg agtcaaagac gtagtcgttg caagtccgca ccaggtactg
     4681 atatcccacc aaaaagtgcg gcggcggctg gcggtagagg ggccagcgta gggtggccgg
     4741 ggctccgggg gcgaggtctt ccaacataag gcgatgatat ccgtagatgt acctggacat
     4801 ccaggtgatg ccggcggcgg tggtggaggc gcgcggaaag tcgcggacgc ggttccagat
     4861 gttgcgcagc ggcaaaaagt gctccatggt cgggacgctc tggccggtga ggcgtgcgca
     4921 gtcgttgacg ctctagaccg tgcaaaagga gagcctgtaa gcgggcactc ttccgtggtc
     4981 tggtggataa attcgcaagg gtatcatggc ggacgaccgg ggttcgaacc ccggatccgg
     5041 ccgtccgccg tgatccatcc ggttaccgcc cgcgtgtcga acccaggtgt gcgacgtcag
     5101 acaacggggg agcgctcctt ttggcttcct tccaggcgcg gcggctgctg cgctagcttt
     5161 tttggcgagc tcgaattaat tctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     5221 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     5281 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     5341 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     5401 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     5461 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     5521 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     5581 tttctccctt cgggaagcgt ggcgctttct caatgctcac gctgtaggta tctcagttcg
     5641 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     5701 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     5761 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     5821 ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct
     5881 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     5941 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     6001 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     6061 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     6121 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     6181 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     6241 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     6301 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     6361 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     6421 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     6481 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     6541 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     6601 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     6661 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     6721 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     6781 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     6841 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     6901 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     6961 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     7021 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     7081 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     7141 cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta
     7201 acctataaaa ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt
     7261 gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc
     7321 gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt
     7381 aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg
     7441 cacagatgcg taaggagaaa ataccgcatc aggcgccatt cgccattcag gctgcgcaac
     7501 tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga
     7561 tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa
     7621 acgacggcca gtgccaagct t
//
