LOCUS       sequence-202634-         906 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of human alpha synuclein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 906)
  AUTHORS   Mbefo MK, Fares MB, Paleologou K, Oueslati A, Yin G, Tenreiro S, 
            Pinto M, Outeiro T, Zweckstetter M, Masliah E, Lashuel HA
  TITLE     Parkinson disease mutant E46K enhances alpha-synuclein 
            phosphorylation in mammalian cell lines, in yeast, and in vivo.
  JOURNAL   J Biol Chem. 2015 Apr 10;290(15):9412-27. doi: 
            10.1074/jbc.M114.610774. Epub 2015 Feb 5.
  PUBMED    25657004
REFERENCE   2  (bases 1 to 906)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M114.610774"; journalName: "J Biol Chem"; date: 
            "2015-04-10- 10"; volume: "290"; issue: "15"; pages: "9412-27"
COMMENT     Sequence Label: pcDNA6 asyn WT
FEATURES             Location/Qualifiers
     source          1..906
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(534..558)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(605..622)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    611..835
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 atattttgtt tactttaaga aggagatata catatggatg tattcatgaa aggactttca
       61 aaggccaagg agggagttgt ggctgctgct gagaaaacca aacagggtgt ggcagaagca
      121 gcaggaaaga caaaagaggg tgttctctat gtaggctcca aaaccaagga gggagtggtg
      181 catggtgtgg caacagtggc tgagaagacc aaagagcaag tgacaaatgt tggaggagca
      241 gtggtgacgg gtgtgacagc agtagcccag aagacagtgg agggagcagg gagcattgca
      301 gcagccactg gctttgtcaa aaaggaccag ttgggcaaga atgaagaagg agccccacag
      361 gaaggaattc tggaagatat gcctgtggat cctgacaatg aggcttatga aatgccttct
      421 gaggaagggt atcaagacta cgaacctgaa gcctaagaaa tatctttgct cccagtttct
      481 tgagatctgc tgacagatgt tccatccaag cttcgagaag ggtgggcgcg ccgacccagc
      541 tttcttgtac aaagtggtga taattaatta agatctagag ggcccgttta aacccgctga
      601 tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct
      661 tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca
      721 tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag
      781 ggggaggatt gggaagacaa tagcangcat gctggggatg cggtgggctc tatggcttct
      841 gaggcggaaa gaaccagctg gggctctagg gggtatcccc acgcgccctg tancggcgca
      901 ttaagc
//
