LOCUS       sequence-202635-        7734 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Calcium channel beta1b auxillary subunit in mammalian 
            cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7734)
  AUTHORS   Page KM, Stephens GJ, Berrow NS, Dolphin AC
  TITLE     The intracellular loop between domains I and II of the B-type 
            calcium channel confers aspects of G-protein sensitivity to the 
            E-type calcium channel.
  JOURNAL   J Neurosci. 1997 Feb 15;17(4):1330-8.
  PUBMED    9006976
REFERENCE   2  (bases 1 to 7734)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J 
            Neurosci."; date: "1997-02-15"; volume: "17(4)"; pages: "1330-8"
COMMENT     Sequence Label: cacnb1b (rat) in pMT2 vector
FEATURES             Location/Qualifiers
     source          1..7734
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(7..336)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      21..156
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     321..341
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     intron          805..1034
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     1017..1036
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             3782..4345
                     /codon_start=1
                     /gene="Mus musculus Dhfr"
                     /product="mouse dihydrofolate reductase"
                     /label=DHFR
                     /translation="MVRPLNCIVAVSQNMGIGKNGDLPWPPLRNEFKYFQRMTTTSSVE
                     GKQNLVIMGRKTWFSIPEKNRPLKDRINIVLSRELKEPPRGAHFLAKSLDDALRLIEQP
                     ELASKVDMVWIVGGSSVYQEAMNQPGHLRLFVTRIMQEFESDTFFPEIDLGKYKLLPEY
                     PGVLSEVQEEKGIKYKFEVYEKKD"
     polyA_signal    4533..4667
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4570..4589)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4624..4643
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5340..5357)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5511..6099)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5591..5610)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6270..7061,7062..7130))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6893..6912
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7131..7235)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7303..7321
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(7359..7381)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     7481..7500
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7694..7716
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7708..7725
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7709..7725
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 aagctttttg caaaagccta ggcctccaaa aaagcctcct cactacttct ggaatagctc
       61 agaggccgag gcggcctcgg cctctgcata aataaaaaaa attagtcagc catggggcgg
      121 agaatgggcg gaactgggcg gagttagggg cgggatgggc ggagttaggg gcgggactat
      181 ggttgctgac taattgagat gcatgctttg catacttctg cctgctgggg agcctgggga
      241 ctttccacac ctggttgctg actaattgag atgcatgctt tgcatacttc tgcctgctgg
      301 ggagcctggg gactttccac accctaactg acacacattc cacaggatcc ggtcgcgcga
      361 atttcgagcg gtgttccgcg gtcctcctcg tatagaaact cggaccactc tgagacgaag
      421 gctcgcgtcc aggccagcac gaaggaggct aagtgggagg ggtagcggtc gttgtccact
      481 agggggtcca ctcgctccag ggtgtgaaga cacatgtcgc cctcttcggc atcaaggaag
      541 gtgattggtt tataggtgta ggccacgtga ccgggtgttc ctgaaggggg gctataaaag
      601 ggggtggggg cgcgttcgtc ctcactctct tccgcatcgc tgtctgcgag ggccagctgt
      661 tgggctcgcg gttgaggaca aactcttcgc ggtctttcca gtactcttgg atcggaaacc
      721 cgtcggcctc cgaacggtac tccgccaccg agggacctga gcgagtccgc atcgaccgga
      781 tcggaaaacc tctcgactgt tggggtgagt actccctctc aaaagcgggc atgacttctg
      841 cgctaagatt gtcagtttcc aaaaacgagg aggatttgat attcacctgg cccgcggtga
      901 tgcctttgag ggtggccgcg tccatctggt cagaaaagac aatctttttg ttgtcaagct
      961 tgaggtgtgg caggcttgag atctggccat acacttgagt gacaatgaca tccactttgc
     1021 ctttctctcc acaggtgtcc actcccaggt ccaactgcag gcgagcctga attcggcacg
     1081 agagcgtgca gctgggcgac tgcacccggt gctggctgcg cgacgccgcg ctgctctcgg
     1141 ctcggacggc ctctcccatg cgctgagggc ccccggctgt gccgggaggg cggccggacc
     1201 ggaggatcct ctccatggtc cagaagagcg gcatgtcccg gggcccttac ccaccttccc
     1261 aagagatccc tatggaggtc ttcgacccca gcccacaggg caaatacagc aagaggaaag
     1321 ggcggttcaa aaggtcagat gggagcacct cctcagatac cacatccaac agctttgtcc
     1381 gtcagggctc agcagagtcc tacacgagcc ggccgtcaga ctccgacgtg tccctggagg
     1441 aggaccggga agccttaagg aaggaggcag agcgccaggc cttagcccag ctcgagaaag
     1501 ccaagaccaa accagtggcc tttgctgttc ggacaaatgt tggctacaat ccgtctccag
     1561 gagatgaggt gccagtgcag ggagtggcca tcacctttga gcccaaggac ttcctgcaca
     1621 tcaaggagaa atacaataat gactggtgga tcgggaggct ggtgaaggaa ggctgtgagg
     1681 ttggtttcat ccccagccct gtcaaactgg acagccttcg cctgctgcag gaacagacgc
     1741 tgcgccaaaa ccgcctcagc tccagcaagt caggtgacaa ctccagttcc agtctgggag
     1801 atgtggtgac tggcacccgc cgccccacac cccccgccag tgccaaacag aagcagaagt
     1861 cgacagagca cgtgcccccc tatgacgtgg tgccttccat gaggcccatc atcctggtgg
     1921 gaccatcgct caagggctat gaggtaactg acatgatgca gaaggcgttg tttgacttct
     1981 tgaagcatcg gtttgatggc aggatttcca tcacccgggt aacggctgac atttccctgg
     2041 ccaagcgctc ggtcctcaac aaccccagca aacacattat catcgagcgc tccaacactc
     2101 gctccagcct ggctgaggta cagagtgaaa tcgagcggat cttcgagctg gcccggacct
     2161 tgcagctggt tgccctggac gccgacacca tcaaccaccc agcccagctc tctaaaacct
     2221 cgctggcccc catcattgtt tacatcaaga tcacatcccc caaggtactg cagaggctca
     2281 tcaaatcccg agggaagtct caatccaaac acctcaatgt ccaaatagca gcctcggaga
     2341 agctggcaca gtgtcccccc gaaatgtttg acataatcct ggacgagaac caattggaag
     2401 atgcctgcga gcacctggcg gagtacttgg aagcctactg gaaggccaca cacccgccta
     2461 gcagcacgcc acccaatccg ctgctgaacc gcaccatggc taccgccgct ctggctgcca
     2521 gccctgcccc cgtctccaac ctccagggac cctaccttgc ttccggggac cagccgctgg
     2581 accgggccac tggggagcat gccagtgtgc acgagtaccc cggggagctg ggccagcccc
     2641 caggccttta ccccagcaac cacccacctg gccgggcagg caccctgcgg gcgctatccc
     2701 gccaagacac ctttgatgct gacacccccg gcagccgaaa ctctgcctac acggagccag
     2761 gagactcgtg tgtggacatg gagacagacc cctcagaggg cccagggcct ggagaccctg
     2821 cagggggagg tacaccacca gctcggcagg gctcctggga agaggaggaa gattatgagg
     2881 aggagatgac cgacaacagg aaccggggcc ggaataaggc ccgctactgt gcggagggtg
     2941 gtgggccggt tctggggcgc aataagaatg agctggaggg ctggggacaa ggcgtctaca
     3001 tccgctgaga ggcaggggca gttctgggag agaagaggct ctgaactcgg ggaggggagg
     3061 gcagaggggc gcaccaccca agatgtgtct tgcctccagg gggcgctttg tccctcattt
     3121 tcagatgcct ttgctcaaag cttggggttt ctttggtggt tatcattccc aactcctggg
     3181 aggtccttaa tccccagctg taggtttttc cctaaactgc tgtgggtgga tgggggaata
     3241 cctactttct gaagtgcccc ctttccccat ctttggggct ttctccctcc cagagaaaag
     3301 gtgcacttcc ttaacttttt actcggggct gtaagcggag gtcctaattg gggtggggtg
     3361 gaggggtttc ttctaccaac gcaggggtac ttgggaaagg ttgggtgctt ttcctgaaga
     3421 gaacccgctg aatcttccac aaaccccaga cccgggatcc cccaccatat ccattccata
     3481 acctagtttc ctagcagcat tctctggtgg gtgaaagcca cagcttggaa acccatgaag
     3541 tgcttgcttt ggggagcaga gctgggtgca aactgacggg catcagtgtg tgctctctgc
     3601 cctccatggt catggcaaga aagcagggcc tagaaacttg aggagtgagc aactcaagat
     3661 gctgcctcgt gccgaattcc gggggggggg ggggggggga cagctcaggg ctgcgatttc
     3721 gcgccaaact tgacggcaat cctagcgtga aggctggtag gattttatcc ccgctgccat
     3781 catggttcga ccattgaact gcatcgtcgc cgtgtcccaa aatatgggga ttggcaagaa
     3841 cggagaccta ccctggcctc cgctcaggaa cgagttcaag tacttccaaa gaatgaccac
     3901 aacctcttca gtggaaggta aacagaatct ggtgattatg ggtaggaaaa cctggttctc
     3961 cattcctgag aagaatcgac ctttaaagga cagaattaat atagttctca gtagagaact
     4021 caaagaacca ccacgaggag ctcattttct tgccaaaagt ttggatgatg ccttaagact
     4081 tattgaacaa ccggaattgg caagtaaagt agacatggtt tggatagtcg gaggcagttc
     4141 tgtttaccag gaagccatga atcaaccagg ccacctcaga ctctttgtga caaggatcat
     4201 gcaggaattt gaaagtgaca cgtttttccc agaaattgat ttggggaaat ataaacttct
     4261 cccagaatac ccaggcgtcc tctctgaggt ccaggaggaa aaaggcatca agtataagtt
     4321 tgaagtctac gagaagaaag actaacagga agatgctttc aagttctctg ctcccctcct
     4381 aaagctatgc attttttata agaccatggg acttttgctg gctttagatc ataatcagcc
     4441 ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc
     4501 tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt
     4561 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     4621 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatcccc ggccaacggt
     4681 ctggtgaccc ggctgcgaga gctcggtgta cctgagacgc gagtaagccc ttgagtcaaa
     4741 gacgtagtcg ttgcaagtcc gcaccaggta ctgatatccc accaaaaagt gcggcggcgg
     4801 ctggcggtag aggggccagc gtagggtggc cggggctccg ggggcgaggt cttccaacat
     4861 aaggcgatga tatccgtaga tgtacctgga catccaggtg atgccggcgg cggtggtgga
     4921 ggcgcgcgga aagtcgcgga cgcggttcca gatgttgcgc agcggcaaaa agtgctccat
     4981 ggtcgggacg ctctggccgg tgaggcgtgc gcagtcgttg acgctctaga ccgtgcaaaa
     5041 ggagagcctg taagcgggca ctcttccgtg gtctggtgga taaattcgca agggtatcat
     5101 ggcggacgac cggggttcga accccggatc cggccgtccg ccgtgatcca tccggttacc
     5161 gcccgcgtgt cgaacccagg tgtgcgacgt cagacaacgg gggagcgctc cttttggctt
     5221 ccttccaggc gcggcggctg ctgcgctagc ttttttggcg agctcgaatt aattctgcat
     5281 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     5341 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     5401 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     5461 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     5521 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     5581 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     5641 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     5701 tctcaatgct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     5761 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     5821 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     5881 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     5941 tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     6001 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     6061 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     6121 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     6181 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     6241 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     6301 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     6361 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     6421 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     6481 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     6541 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     6601 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     6661 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     6721 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     6781 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     6841 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     6901 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     6961 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     7021 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     7081 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     7141 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     7201 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     7261 gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg
     7321 ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg
     7381 gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg
     7441 tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta
     7501 ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc
     7561 atcaggcgcc attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc
     7621 tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta
     7681 acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gctt
//
