LOCUS       sequence-202637-       14784 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pSC218GG-Glycine max Ubiquitin:Gateway expression cassette binary 
            vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14784)
  AUTHORS   Curtin SJ, Xiong Y, Michno JM, Campbell BW, Stec AO, Cermak T, 
            Starker C, Voytas DF, Eamens AL, Stupar RM
  TITLE     CRISPR/Cas9 and TALENs generate heritable mutations for genes 
            involved in small RNA processing of Glycine max and Medicago 
            truncatula.
  JOURNAL   Plant Biotechnol J. 2017 Oct 31. doi: 10.1111/pbi.12857.
  PUBMED    29087011
REFERENCE   2  (bases 1 to 14784)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Biotechnol J. 2017 Oct 31. doi: 10.1111/pbi.12857."
COMMENT     Sequence Label: pSC5
FEATURES             Location/Qualifiers
     source          1..14784
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(126..497)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            530..639
                     /note="incP origin of transfer"
     primer_bind     862..881
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1115..1132
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(1320..1970)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    2435..2459
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     protein_bind    3667..3791
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(3816..3821,3822..3839,3840..3846)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             3900..4580
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQAGRNLE
                     DPAY"
     primer_bind     complement(3966..3985)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             4900..5205
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     5084..5103
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(5246..5370)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      5408..6115
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     promoter        6665..7010
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     6953..6973
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             7031..7582
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     terminator      7726..7978
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(7991..8008)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7991..8007)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8000..8022)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(8147..8166)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8357..8378
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    8625..8649
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(10245..11393)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(11692..12486)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  12678..13445
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      14138..14773
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 cggtcttgcc ttgctcgtcg gtgatgtact tcaccagctc cgcgaagtcg ctcttcttga
       61 tggagcgcat ggggacgtgc ttggcaatca cgcgcacccc ccggccgttt tagcggctaa
      121 aaaagtcatg gctctgccct cgggcggacc acgcccatca tgaccttgcc aagctcgtcc
      181 tgcttctctt cgatcttcgc cagcagggcg aggatcgtgg catcaccgaa ccgcgccgtg
      241 cgcgggtcgt cggtgagcca gagtttcagc aggccgccca ggcggcccag gtcgccattg
      301 atgcgggcca gctcgcggac gtgctcatag tccacgacgc ccgtgatttt gtagccctgg
      361 ccgacggcca gcaggtaggc cgacaggctc atgccggccg ccgccgcctt ttcctcaatc
      421 gctcttcgtt cgtctggaag gcagtacacc ttgataggtg ggctgccctt cctggttggc
      481 ttggtttcat cagccatccg cttgccctca tctgttacgc cggcggtagc cggccagcct
      541 cgcagagcag gattcccgtt gagcaccgcc aggtgcgaat aagggacagt gaagaaggaa
      601 cacccgctcg cgggtgggcc tacttcacct atcctgcccg gctgacgccg ttggatacac
      661 caaggaaagt ctacacgaac cctttggcaa aatcctgtat atcgtgcgaa aaaggatgga
      721 tataccgaaa aaatcgctat aatgaccccg aagcagggtt atgcagcgga aaagcgccac
      781 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
      841 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
      901 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
      961 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     1021 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     1081 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
     1141 agagcgccag aaggccgcca gagaggccga gcgcggccgt gaggcttgga cgctagggca
     1201 gggcatgaaa aagcccgtag cgggctgcta cgggcgtctg acgcggtgga aagggggagg
     1261 ggatgttgtc tacatggctc tgctgtagtg agtgggttgc gctccggcag cggtcctgat
     1321 caatcgtcac cctttctcgg tccttcaacg ttcctgacaa cgagcctcct tttcgccaat
     1381 ccatcgacaa tcaccgcgag tccctgctcg aacgctgcgt ccggaccggc ttcgtcgaag
     1441 gcgtctatcg cggcccgcaa cagcggcgag agcggagcct gttcaacggt gccgccgcgc
     1501 tcgccggcat cgctgtcgcc ggcctgctcc tcaagcacgg ccccaacagt gaagtagctg
     1561 attgtcatca gcgcattgac ggcgtccccg gccgaaaaac ccgcctcgca gaggaagcga
     1621 agctgcgcgt cggccgtttc catctgcggt gcgcccggtc gcgtgccggc atggatgcgc
     1681 gcgccatcgc ggtaggcgag cagcgcctgc ctgaagctgc gggcattccc gatcagaaat
     1741 gagcgccagt cgtcgtcggc tctcggcacc gaatgcgtat gattctccgc cagcatggct
     1801 tcggccagtg cgtcgagcag cgcccgcttg ttcctgaagt gccagtaaag cgccggctgc
     1861 tgaaccccca accgttccgc cagtttgcgt gtcgtcagac cgtctacgcc gacctcgttc
     1921 aacaggtcca gggcggcacg gatcactgta ttcggctgca actttgtcat gcttgacact
     1981 ttatcactga taaacataat atgtccacca acttatcagt gataaagaat ccgcgcgttc
     2041 aatcggacca gcggaggctg gtccggaggc cagacgtgaa acccaacata cccctgatcg
     2101 taattctgag cactgtcgcg ctcgacgctg tcggcatcgg cctgattatg ccggtgctgc
     2161 cgggcctcct gcgcgatctg gttcactcga acgacgtcac cgcccactat ggcattctgc
     2221 tggcgctgta tgcgttggtg caatttgcct gcgcacctgt gctgggcgcg ctgtcggatc
     2281 gtttcgggcg gcggccaatc ttgctcgtct cgctggccgg cgccagatct ggggaaccct
     2341 gtggttggca tgcacataca aatggacgaa cggataaacc ttttcacgcc cttttaaata
     2401 tccgattatt ctaataaacg ctcttttctc ttaggtttac ccgccaatat atcctgtcaa
     2461 acactgatag tttaaactga aggcgggaaa cgacaatctg atcatgagcg gagaattaag
     2521 ggagtcacgt tatgaccccc gccgatgacg cgggacaagc cgttttacgt ttggaactga
     2581 cagaaccgca acgttgaagg agccactcag ccgcgggttt ctggagttta atgagctaag
     2641 cacatacgtc agaaaccatt attgcgcgtt caaaagtcgc ctaaggtcac tatcagctag
     2701 acggacgcgg ccgcgggccc aatataacaa cgacgtcgta acagataaag cgaagcttga
     2761 aggtgcatgt gactccgtca agattacgaa accgccaact accacgcaaa ttgcaattct
     2821 caatttccta gaaggactct ccgaaaatgc atccaatacc aaatattacc cgtgtcatag
     2881 gcaccaagtg acaccataca tgaacacgcg tcacaatatg actggagaag ggttccacac
     2941 cttatgctat aaaacgcccc acacccctcc tccttccttc gcagttcaat tccaatatat
     3001 tccattctct ctgtgtattt ccctacctct cccttcaagg ttagtcgatt tcttctgttt
     3061 ttcttcttcg ttctttccat gaattgtgta tgttctttga tcaatacgat gttgatttga
     3121 ttgtgttttg tttggtttca tcgatcttca attttcataa tcagattcag cttttattat
     3181 ctttacaaca acgtccttaa tttgatgatt ctttaatcgt agatttgctc taattagagc
     3241 tttttcatgt cagatccctt tacaacaagc cttaattgtt gattcattaa tcgtagatta
     3301 gggctttttt cattgattac ttcagatccg ttaaacgtaa ccatagatca gggctttttc
     3361 atgaattact tcagatccgt taaacaacag ccttattttt tatacttctg tggtttttca
     3421 agaaattgtt cagatccgtt gacaaaaagc cttattcgtt gattctatat cgtttttcga
     3481 gagatattgc tcagatctgt tagcaactgc cttgtttgtt gattctattg ccgtggatta
     3541 gggttttttt tcacgagatt gcttcagatc cgtacttaag attacgtaat ggattttgat
     3601 tctgatttat ctgtgattgt tgactcgaca ggtaccttca aacctcgagg cgcgccaagc
     3661 tatcaaacaa gtttgtacaa aaaagctgaa cgagaaacgt aaaatgatat aaatatcaat
     3721 atattaaatt agattttgca taaaaaacag actacataat actgtaaaac acaacatatc
     3781 cagtcactat ggcggccgca ttaggcaccc caggctttac actttatgct tccggctcgt
     3841 ataatgtgtg gattttgagt taggatccgt cgagattttc aggagctaag gaagctaaaa
     3901 tggagaaaaa aatcactgga tataccaccg ttgatatatc ccaatggcat cgtaaagaac
     3961 attttgaggc atttcagtca gttgctcaat gtacctataa ccagaccgtt cagctggata
     4021 ttacggcctt tttaaagacc gtaaagaaaa ataagcacaa gttttatccg gcctttattc
     4081 acattcttgc ccgcctgatg aatgctcatc cggaattccg tatggcaatg aaagacggtg
     4141 agctggtgat atgggatagt gttcaccctt gttacaccgt tttccatgag caaactgaaa
     4201 cgttttcatc gctctggagt gaataccacg acgatttccg gcagtttcta cacatatatt
     4261 cgcaagatgt ggcgtgttac ggtgaaaacc tggcctattt ccctaaaggg tttattgaga
     4321 atatgttttt cgtctcagcc aatccctggg tgagtttcac cagttttgat ttaaacgtgg
     4381 ccaatatgga caacttcttc gcccccgttt tcaccatggg caaatattat acgcaaggcg
     4441 acaaggtgct gatgccgctg gcgattcagg ttcatcatgc cgtttgtgat ggcttccatg
     4501 tcggcagaat gcttaatgaa ttacaacagt actgcgatga gtggcaggcg gggcgtaatc
     4561 tagaggatcc ggcttactaa aagccagata acagtatgcg tatttgcgcg ctgatttttg
     4621 cggtataaga atatatactg atatgtatac ccgaagtatg tcaaaaagag gtatgctatg
     4681 aagcagcgta ttacagtgac agttgacagc gacagctatc agttgctcaa ggcatatatg
     4741 atgtcaatat ctccggtctg gtaagcacaa ccatgcagaa tgaagcccgt cgtctgcgtg
     4801 ccgaacgctg gaaagcggaa aatcaggaag ggatggctga ggtcgcccgg tttattgaaa
     4861 tgaacggctc ttttgctgac gagaacaggg gctggtgaaa tgcagtttaa ggtttacacc
     4921 tataaaagag agagccgtta tcgtctgttt gtggatgtac agagtgatat tattgacacg
     4981 cccgggcgac ggatggtgat ccccctggcc agtgcacgtc tgctgtcaga taaagtcccc
     5041 cgtgaacttt acccggtggt gcatatcggg gatgaaagct ggcgcatgat gaccaccgat
     5101 atggccagtg tgccggtctc cgttatcggg gaagaagtgg ctgatctcag ccaccgcgaa
     5161 aatgacatca aaaacgccat taacctgatg ttctggggaa tataaatgtc aggctccctt
     5221 atacacagcc agtctgcagg tcgaccatag tgactggata tgttgtgttt tacagtatta
     5281 tgtagtctgt tttttatgca aaatctaatt taatatattg atatttatat cattttacgt
     5341 ttctcgttca gctttcttgt acaaagtggt tcgataattc ttaattaact agttctaatc
     5401 tagagtcctg ctttaatgag atatgcgaga cgcctatgat cgcatgatat ttgctttcaa
     5461 ttctgttgtg cacgttgtaa aaaacctgag catgtgtagc tcagatcctt accgccggtt
     5521 tcggttcatt ctaatgaata tatcacccgt tactatcgta tttttatgaa taatattctc
     5581 cgttcaattt actgattgta ccctactact tatatgtaca atattaaaat gaaaacaata
     5641 tattgtgctg aataggttta tagcgacatc tatgatagag cgccacaata acaaacaatt
     5701 gcgttttatt attacaaatc caattttaaa aaaagcggca gaaccggtca aacctaaaag
     5761 actgattaca taaatcttat tcaaatttca aaagtgcccc aggggctagt atctacgaca
     5821 caccgagcgg cgaactaata acgctcactg aagggaactc cggttccccg ccggcgcgca
     5881 tgggtgagat tccttgaagt tgagtattgg ccgtccgctc taccgaaagt tacgggcacc
     5941 attcaacccg gtccagcacg gcggccgggt aaccgacttg ctgccccgag aattatgcag
     6001 catttttttg gtgtatgtgg gccccaaatg aagtgcaggt caaaccttga cagtgacgac
     6061 aaatcgttgg gcgggtccag ggcgaatttt gcgacaacat gtcgaggctc agcaggacct
     6121 gcaggcatgc aagctagtga agaatcgatt tgctacgtat tgcccctgca ggtccccaga
     6181 ttagcctttt caatttcaga aagaatgcta acccacagat ggttagagag gcttacgcag
     6241 caggtctcat caagacgatc tacccgagca ataatctcca ggaaatcaaa taccttccca
     6301 agaaggttaa agatgcagtc aaaagattca ggactaactg catcaagaac acagagaaag
     6361 atatatttct caagatcaga agtactattc cagtatggac gattcaaggc ttgcttcaca
     6421 aaccaaggca agtaatagag attggagtct ctaaaaaggt agttcccact gaatcaaagg
     6481 ccatggagtc aaagattcaa atagaggacc taacagaact cgccgtaaag actggcgaac
     6541 agttcataca gagtctctta cgactcaatg acaagaagaa aatcttcgtc aacatggtgg
     6601 agcacgacac acttgtctac tccaaaaata tcaaagatac agtctcagaa gaccaaaggg
     6661 caattgagac ttttcaacaa agggtaatat ccggaaacct cctcggattc cattgcccag
     6721 ctatctgtca ctttattgtg aagatagtgg aaaaggaagg tggctcctac aaatgccatc
     6781 attgcgataa aggaaaggcc atcgttgaag atgcctctgc cgacagtggt cccaaagatg
     6841 gacccccacc cacgaggagc atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc
     6901 aagtggattg atgtgatatc tccactgacg taagggatga cgcacaatcc cactatcctt
     6961 cgcaagaccc ttcctctata taaggaagtt catttcattt ggagagaaca cgggggactc
     7021 tagaggatcc atgagcccag aacgacgccc ggccgacatc cgccgtgcca ccgaggcgga
     7081 catgccggcg gtctgcacca tcgtcaacca ctacatcgag acaagcacgg tcaacttccg
     7141 taccgagccg caggaaccgc aggagtggac ggacgacctc gtccgtctgc gggagcgcta
     7201 tccctggctc gtcgccgagg tggacggcga ggtcgccggc atcgcctacg cgggcccctg
     7261 gaaggcacgc aacgcctacg actggacggc cgagtcgacc gtgtacgtct ccccccgcca
     7321 ccagcggacg ggactgggct ccacgctcta cacccacctg ctgaagtccc tggaggcaca
     7381 gggcttcaag agcgtggtcg ctgtcatcgg gctgcccaac gacccgagcg tgcgcatgca
     7441 cgaggcgctc ggatatgccc cccgcggcat gctgcgggcg gccggcttca agcacgggaa
     7501 ctggcatgac gtgggtttct ggcagctgga cttcagcctg ccggtaccgc cccgtccggt
     7561 cctgcccgtc accgagatct gaacggagtg cgcgtggcat cgcccgagtt ggagctggta
     7621 cgggaactca tcgaactcaa ctggcatacc cgcaatggtg aggtggaacc gcggcggatc
     7681 gcgtacgacc gtgcccagga gggggtaccg agctcgaatt tccccgatcg ttcaaacatt
     7741 tggcaataaa gtttcttaag attgaatcct gttgccggtc ttgcgatgat tatcatataa
     7801 tttctgttga attacgttaa gcatgtaata attaacatgt aatgcatgac gttatttatg
     7861 agatgggttt ttatgattag agtcccgcaa ttatacattt aatacgcgat agaaaacaaa
     7921 atatagcgcg caaactagga taaattatcg cgcgcggtgt catctatgtt actagatcgg
     7981 gaattaattc actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc
     8041 aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc
     8101 gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgcccgctcc tttcgctttc
     8161 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     8221 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgatttgggt
     8281 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     8341 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     8401 ggctattctt ttgatttata agggattttg ccgatttcgg aaccaccatc aaacaggatt
     8461 ttcgcctgct ggggcaaacc agcgtggacc gcttgctgca actctctcag ggccaggcgg
     8521 tgaagggcaa tcagctgttg cccgtctcac tggtgaaaag aaaaaccacc ccagtacatt
     8581 aaaaacgtcc gcaatgtgtt attaagttgt ctaagcgtca atttgtttac accacaatat
     8641 atcctgccac cagccagcca acagctcccc gaccggcagc tcggcacaaa atcaccactc
     8701 gatacaggca gcccatcagt ccgggacggc gtcagcggga gagccgttgt aaggcggcag
     8761 actttgctca tgttaccgat gctattcgga agaacggcaa ctaagctgcc gggtttgaaa
     8821 cacggatgat ctcgcggagg gtagcatgtt gattgtaacg atgacagagc gttgctgcct
     8881 gtgatcaaat atcatctccc tcgcagagat ccgaattatc agccttctta ttcatttctc
     8941 gcttaaccgt gacaggctgt cgatcttgag aactatgccg acataatagg aaatcgctgg
     9001 ataaagccgc tgaggaagct gagtggcgct atttctttag aagtgaacgt tgacgatatc
     9061 aactccccta tccattgctc accgaatggt acaggtcggg gacccgaagt tccgactgtc
     9121 ggcctgatgc atccccggct gatcgacccc agatctgggg ctgagaaagc ccagtaagga
     9181 aacaactgta ggttcgagtc gcgagatccc ccggaaccaa aggaagtagg ttaaacccgc
     9241 tccgatcagg ccgagccacg ccaggccgag aacattggtt cctgtaggca tcgggattgg
     9301 cggatcaaac actaaagcta ctggaacgag cagaagtcct ccggccgcca gttgccaggc
     9361 ggtaaaggtg agcagaggca cgggaggttg ccacttgcgg gtcagcacgg ttccgaacgc
     9421 catggaaacc gcccccgcca ggcccgctgc gacgccgaca ggatctagcg ctgcgtttgg
     9481 tgtcaacacc aacagcgcca cgcccgcagt tccgcaaata gcccccagga ccgccatcaa
     9541 tcgtatcggg ctacctagca gagcggcaga gatgaacacg accatcagcg gctgcacagc
     9601 gcctaccgtc gccgcgaccc cgcccggcag gcggtagacc gaaataaaca acaagctcca
     9661 gaatagcgaa atattaagtg cgccgaggat gaagatgcgc atccaccaga ttcccgttgg
     9721 aatctgtcgg acgatcatca cgagcaataa acccgccggc aacgcccgca gcagcatacc
     9781 ggcgacccct cggcctcgct gttcgggctc cacgaaaacg ccggacagat gcgccttgtg
     9841 agcgtccttg gggccgtcct cctgtttgaa gaccgacagc ccaatgatct cgccgtcgat
     9901 gtaggcgccg aatgccacgg catctcgcaa ccgttcagcg aacgcctcca tgggcttttt
     9961 ctcctcgtgc tcgtaaacgg acccgaacat ctctggagct ttcttcaggg ccgacaatcg
    10021 gatctcgcgg aaatcctgca cgtcggccgc tccaagccgt cgaatctgag ccttaatcac
    10081 aattgtcaat tttaatcctc tgtttatcgg cagttcgtag agcgcgccgt gcgtcccgag
    10141 cgatactgag cgaagcaagt gcgtcgagca gtgcccgctt gttcctgaaa tgccagtaaa
    10201 gcgctggctg ctgaaccccc agccggaact gaccccacaa ggccctagcg tttgcaatgc
    10261 accaggtcat cattgaccca ggcgtgttcc accaggccgc tgcctcgcaa ctcttcgcag
    10321 gcttcgccga cctgctcgcg ccacttcttc acgcgggtgg aatccgatcc gcacatgagg
    10381 cggaaggttt ccagcttgag cgggtacggc tcccggtgcg agctgaaata gtcgaacatc
    10441 cgtcgggccg tcggcgacag cttgcggtac ttctcccata tgaatttcgt gtagtggtcg
    10501 ccagcaaaca gcacgacgat ttcctcgtcg atcaggacct ggcaacggga cgttttcttg
    10561 ccacggtcca ggacgcggaa gcggtgcagc agcgacaccg attccaggtg cccaacgcgg
    10621 tcggacgtga agcccatcgc cgtcgcctgt aggcgcgaca ggcattcctc ggccttcgtg
    10681 taataccggc cattgatcga ccagcccagg tcctggcaaa gctcgtagaa cgtgaaggtg
    10741 atcggctcgc cgataggggt gcgcttcgcg tactccaaca cctgctgcca caccagttcg
    10801 tcatcgtcgg cccgcagctc gacgccggtg taggtgatct tcacgtcctt gttgacgtgg
    10861 aaaatgacct tgttttgcag cgcctcgcgc gggattttct tgttgcgcgt ggtgaacagg
    10921 gcagagcggg ccgtgtcgtt tggcatcgct cgcatcgtgt ccggccacgg cgcaatatcg
    10981 aacaaggaaa gctgcatttc cttgatctgc tgcttcgtgt gtttcagcaa cgcggcctgc
    11041 ttggcctcgc tgacctgttt tgccaggtcc tcgccggcgg tttttcgctt cttggtcgtc
    11101 atagttcctc gcgtgtcgat ggtcatcgac ttcgccaaac ctgccgcctc ctgttcgaga
    11161 cgacgcgaac gctccacggc ggccgatggc gcgggcaggg cagggggagc cagttgcacg
    11221 ctgtcgcgct cgatcttggc cgtagcttgc tggaccatcg agccgacgga ctggaaggtt
    11281 tcgcggggcg cacgcatgac ggtgcggctt gcgatggttt cggcatcctc ggcggaaaac
    11341 cccgcgtcga tcagttcttg cctgtatgcc ttccggtcaa acgtccgatt cattcaccct
    11401 ccttgcggga ttgccccgac tcacgccggg gcaatgtgcc cttattcctg atttgacccg
    11461 cctggtgcct tggtgtccag ataatccacc ttatcggcaa tgaagtcggt cccgtagacc
    11521 gtctggccgt ccttctcgta cttggtattc cgaatcttgc cctgcacgaa taccagcgac
    11581 cccttgccca aatacttgcc gtgggcctcg gcctgagagc caaaacactt gatgcggaag
    11641 aagtcggtgc gctcctgctt gtcgccggca tcgttgcgcc acatctaggt actaaaacaa
    11701 ttcatccagt aaaatataat attttatttt ctcccaatca ggcttgatcc ccagtaagtc
    11761 aaaaaatagc tcgacatact gttcttcccc gatatcctcc ctgatcgacc ggacgcagaa
    11821 ggcaatgtca taccacttgt ccgccctgcc gcttctccca agatcaataa agccacttac
    11881 tttgccatct ttcacaaaga tgttgctgtc tcccaggtcg ccgtgggaaa agacaagttc
    11941 ctcttcgggc ttttccgtct ttaaaaaatc atacagctcg cgcggatctt taaatggagt
    12001 gtcttcttcc cagttttcgc aatccacatc ggccagatcg ttattcagta agtaatccaa
    12061 ttcggctaag cggctgtcta agctattcgt atagggacaa tccgatatgt cgatggagtg
    12121 aaagagcctg atgcactccg catacagctc gataatcttt tcagggcttt gttcatcttc
    12181 atactcttcc gagcaaagga cgccatcggc ctcactcatg agcagattgc tccagccatc
    12241 atgccgttca aagtgcagga cctttggaac aggcagcttt ccttccagcc atagcatcat
    12301 gtccttttcc cgttccacat cataggtggt ccctttatac cggctgtccg tcatttttaa
    12361 atataggttt tcattttctc ccaccagctt atatacctta gcaggagaca ttccttccgt
    12421 atcttttacg cagcggtatt tttcgatcag ttttttcaat tccggtgata ttctcatttt
    12481 agccatttat tatttccttc ctcttttcta cagtatttaa agatacccca agaagctaat
    12541 tataacaaga cgaactccaa ttcactgttc cttgcattct aaaaccttaa ataccagaaa
    12601 acagcttttt caaagttgtt ttcaaagttg gcgtataaca tagtatcgac ggagccgatt
    12661 ttgaaaccac aattatgggt gatgctgcca acttactgat ttagtgtatg atggtgtttt
    12721 tgaggtgctc cagtggcttc tgtttctatc agctgtccct cctgttcagc tactgacggg
    12781 gtggtgcgta acggcaaaag caccgccgga catcagcgct atctctgctc tcactgccgt
    12841 aaaacatggc aactgcagtt cacttacacc gcttctcaac ccggtacgca ccagaaaatc
    12901 attgatatgg ccatgaatgg cgttggatgc cgggcaacag cccgcattat gggcgttggc
    12961 ctcaacacga ttttacgtca cttaaaaaac tcaggccgca gtcggtaacc tcgcgcatac
    13021 agccgggcag tgacgtcatc gtctgcgcgg aaatggacga acagtggggc tatgtcgggg
    13081 ctaaatcgcg ccagcgctgg ctgttttacg cgtatgacag tctccggaag acggttgttg
    13141 cgcacgtatt cggtgaacgc actatggcga cgctggggcg tcttatgagc ctgctgtcac
    13201 cctttgacgt ggtgatatgg atgacggatg gctggccgct gtatgaatcc cgcctgaagg
    13261 gaaagctgca cgtaatcagc aagcgatata cgcagcgaat tgagcggcat aacctgaatc
    13321 tgaggcagca cctggcacgg ctgggacgga agtcgctgtc gttctcaaaa tcggtggagc
    13381 tgcatgacaa agtcatcggg cattatctga acataaaaca ctatcaataa gttggagtca
    13441 ttacccaatt atgatagaat ttacaagcta taaggttatt gtcctgggtt tcaagcatta
    13501 gtccatgcaa gtttttatgc tttgcccatt ctatagatat attgataagc gcgctgccta
    13561 tgccttgccc cctgaaatcc ttacatacgg cgatatcttc tatataaaag atatattatc
    13621 ttatcagtat tgtcaatata ttcaaggcaa tctgcctcct catcctcttc atcctcttcg
    13681 tcttggtagc tttttaaata tggcgcttca tagagtaatt ctgtaaaggt ccaattctcg
    13741 ttttcatacc tcggtataat cttacctatc acctcaaatg gttcgctggg tttatcgcac
    13801 ccccgaacac gagcacggca cccgcgacca ctatgccaag aatgcccaag gtaaaaattg
    13861 ccggccccgc catgaagtcc gtgaatgccc cgacggccga agtgaagggc aggccgccac
    13921 ccaggccgcc gccctcactg cccggcacct ggtcgctgaa tgtcgatgcc agcacctgcg
    13981 gcacgtcaat gcttccgggc gtcgcgctcg ggctgatcgc ccatcccgtt actgccccga
    14041 tcccggcaat ggcaaggact gccagcgctg ccatttttgg ggtgaggccg ttcgcggccg
    14101 aggggcgcag cccctggggg gatgggaggc ccgcgttagc gggccgggag ggttcgagaa
    14161 gggggggcac cccccttcgg cgtgcgcggt cacgcgcaca gggcgcagcc ctggttaaaa
    14221 acaaggttta taaatattgg tttaaaagca ggttaaaaga caggttagcg gtggccgaaa
    14281 aacgggcgga aacccttgca aatgctggat tttctgcctg tggacagccc ctcaaatgtc
    14341 aataggtgcg cccctcatct gtcagcactc tgcccctcaa gtgtcaagga tcgcgcccct
    14401 catctgtcag tagtcgcgcc cctcaagtgt caataccgca gggcacttat ccccaggctt
    14461 gtccacatca tctgtgggaa actcgcgtaa aatcaggcgt tttcgccgat ttgcgaggct
    14521 ggccagctcc acgtcgccgg ccgaaatcga gcctgcccct catctgtcaa cgccgcgccg
    14581 ggtgagtcgg cccctcaagt gtcaacgtcc gcccctcatc tgtcagtgag ggccaagttt
    14641 tccgcgaggt atccacaacg ccggcggccg gccgcggtgt ctcgcacacg gcttcgacgg
    14701 cgtttctggc gcgtttgcag ggccatagac ggccgccagc ccagcggcga gggcaaccag
    14761 cccggtgagc gtcggaaagg cgct
//
