LOCUS       sequence-20269-e         864 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 864)
  AUTHORS   Jauliac S, Lopez-Rodriguez C, Shaw LM, Brown LF, Rao A, Toker A
  TITLE     The role of NFAT transcription factors in integrin-mediated 
            carcinoma invasion.
  JOURNAL   Nat Cell Biol. 2002 Jul . 4(7):540-4.
  PUBMED    12080349
REFERENCE   2  (bases 1 to 864)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Cell 
            Biol. 2002 Jul . 4(7):540-4."
COMMENT     Sequence Label: pEGFP NFAT5 DBD DM
FEATURES             Location/Qualifiers
     source          1..864
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      18..27
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(24..26,27..29,30..740)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(24..26,27..29,30..740)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(69..90)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(330..349)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     677..698
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gatccgctag cccggtcgcc accatggtga gcaagggcga ggagctgttc accggggtgg
       61 tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg
      121 agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca
      181 agctgcccgt gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca
      241 gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct
      301 acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg
      361 tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg
      421 aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata
      481 tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg
      541 aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc
      601 ccgtgctgct gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca
      661 acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg
      721 gcatggacga gctgtacaag ggggaaggat ccggcgaagg caactcaaaa gcgggaaatg
      781 gaacattgga aaaccaaaaa ggaactggag taaagaagag ccctatgttg tgtggacaat
      841 atcctgttaa aagtgaggga aagg
//
