LOCUS       sequence-202691-       18337 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CRISPR/Cas9 2xplex gRNA targeting Medtr2g044580 (ERDJ2). Also 
            expresses Cas9 from AtUBQ10 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 18337)
  AUTHORS   Curtin SJ, Tiffin P, Guhlin J, Trujillo DI, Burghart LT, Atkins P, 
            Baltes NJ, Denny R, Voytas DF, Stupar RM, Young ND
  TITLE     Validating Genome-Wide Association Candidates Controlling 
            Quantitative Variation in Nodulation.
  JOURNAL   Plant Physiol. 2017 Feb;173(2):921-931. doi: 10.1104/pp.16.01923. 
            Epub 2017 Jan 5.
  PUBMED    28057894
REFERENCE   2  (bases 1 to 18337)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1104/pp.16.01923"; journalName: "Plant Physiol"; date: 
            "2017-02"; volume: "173"; issue: "2"; pages: "921-931"
COMMENT     Sequence Label: pSC14
FEATURES             Location/Qualifiers
     source          1..18337
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    19..43
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             65..112
                     /codon_start=1
                     /product="two tandem FLAG(R) epitope tags"
                     /label=2xFLAG
                     /translation="DYKDDDDKDYKDDDDK"
     CDS             119..139
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(164..1600,1790..4453)
                     /codon_start=1
                     /product="Cas9 endonuclease from the Streptococcus pyogenes
                     Type II CRISPR/Cas system"
                     /label=pcoCas9
                     /note="plant codon-optimized Cas9 gene containing the 
                     potato IV2 intron"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     intron          1601..1789
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
     CDS             4454..4501
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     terminator      4512..4764
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    4809..4829
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     misc_RNA        5184..5259
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    complement(5271..5295)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_RNA        5773..5848
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    complement(5860..5884)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5903..5927)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      5991..6243
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(6261..6277)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6261..6277)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     misc_feature    6543..6567
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             7032..7682
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     primer_bind     complement(7870..7887)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(8121..8140)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     oriT            8363..8472
                     /note="incP origin of transfer"
     CDS             8505..8876
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     rep_origin      9003..9718
                     /label=oriV
                     /note="incP origin of replication"
     mobile_element  10341..11108
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     CDS             11300..12094
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     CDS             12393..13541
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     misc_feature    15137..15161
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     primer_bind     complement(15408..15429)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     15620..15639
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     15779..15795
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      complement(15808..16060)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(16204..16755)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(16776..17121)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(16813..16833)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     primer_bind     17657..17673
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ggcgcgccaa gctatcaaac aagtttgtac aaaaaagcag gctccgaatt cgcccttcac
       61 catggattac aaggatgatg atgataagga ttacaaggat gatgatgata agatggctcc
      121 aaagaagaag agaaaggttg gaatccacgg agttccagct gctgataaga agtactctat
      181 cggacttgac atcggaacca actctgttgg atgggctgtt atcaccgatg agtacaaggt
      241 tccatctaag aagttcaagg ttcttggaaa caccgataga cactctatca agaagaacct
      301 tatcggtgct cttcttttcg attctggaga gaccgctgag gctaccagat tgaagagaac
      361 cgctagaaga agatacacca gaagaaagaa cagaatctgc taccttcagg aaatcttctc
      421 taacgagatg gctaaggttg atgattcttt cttccacaga cttgaggagt ctttccttgt
      481 tgaggaggat aagaagcacg agagacaccc aatcttcgga aacatcgttg atgaggttgc
      541 ttaccacgag aagtacccaa ccatctacca ccttagaaag aagttggttg attctaccga
      601 taaggctgat cttagactta tctaccttgc tcttgctcac atgatcaagt tcagaggaca
      661 cttccttatc gagggagacc ttaacccaga taactctgat gttgataagt tgttcatcca
      721 gcttgttcag acctacaacc agcttttcga ggagaaccca atcaacgctt ctggagttga
      781 tgctaaggct atcctttctg ctagactttc taagtctcgt agacttgaga accttatcgc
      841 tcagcttcca ggagagaaga agaacggact tttcggaaac cttatcgctc tttctcttgg
      901 acttacccca aacttcaagt ctaacttcga tcttgctgag gatgctaagt tgcagctttc
      961 taaggatacc tacgatgatg atcttgataa ccttcttgct cagatcggag atcagtacgc
     1021 tgatcttttc cttgctgcta agaacctttc tgatgctatc cttctttctg acatccttag
     1081 agttaacacc gagatcacca aggctccact ttctgcttct atgatcaaga gatacgatga
     1141 gcaccaccag gatcttaccc ttttgaaggc tcttgttaga cagcagcttc cagagaagta
     1201 caaggaaatc ttcttcgatc agtctaagaa cggatacgct ggatacatcg atggaggagc
     1261 ttctcaggag gagttctaca agttcatcaa gccaatcctt gagaagatgg atggaaccga
     1321 ggagcttctt gttaagttga acagagagga tcttcttaga aagcagagaa ccttcgataa
     1381 cggatctatc ccacaccaga tccaccttgg agagcttcac gctatccttc gtagacagga
     1441 ggatttctac ccattcttga aggataacag agagaagatc gagaagatcc ttaccttcag
     1501 aatcccatac tacgttggac cacttgctag aggaaactct cgtttcgctt ggatgaccag
     1561 aaagtctgag gagaccatca ccccttggaa cttcgaggag gtaagtttct gcttctacct
     1621 ttgatatata tataataatt atcattaatt agtagtaata taatatttca aatatttttt
     1681 tcaaaataaa agaatgtagt atatagcaat tgcttttctg tagtttataa gtgtgtatat
     1741 tttaatttat aacttttcta atatatgacc aaaatttgtt gatgtgcagg ttgttgataa
     1801 gggagcttct gctcagtctt tcatcgagag aatgaccaac ttcgataaga accttccaaa
     1861 cgagaaggtt cttccaaagc actctcttct ttacgagtac ttcaccgttt acaacgagct
     1921 taccaaggtt aagtacgtta ccgagggaat gagaaagcca gctttccttt ctggagagca
     1981 gaagaaggct atcgttgatc ttcttttcaa gaccaacaga aaggttaccg ttaagcagtt
     2041 gaaggaggat tacttcaaga agatcgagtg cttcgattct gttgaaatct ctggagttga
     2101 ggatagattc aacgcttctc ttggaaccta ccacgatctt ttgaagatca tcaaggataa
     2161 ggatttcctt gataacgagg agaacgagga catccttgag gacatcgttc ttacccttac
     2221 ccttttcgag gatagagaga tgatcgagga gagactcaag acctacgctc accttttcga
     2281 tgataaggtt atgaagcagt tgaagagaag aagatacacc ggatggggta gactttctcg
     2341 taagttgatc aacggaatca gagataagca gtctggaaag accatccttg atttcttgaa
     2401 gtctgatgga ttcgctaaca gaaacttcat gcagcttatc cacgatgatt ctcttacctt
     2461 caaggaggac atccagaagg ctcaggtttc tggacaggga gattctcttc acgagcacat
     2521 cgctaacctt gctggatctc cagctatcaa gaagggaatc cttcagaccg ttaaggttgt
     2581 tgatgagctt gttaaggtta tgggtagaca caagccagag aacatcgtta tcgagatggc
     2641 tagagagaac cagaccaccc agaagggaca gaagaactct cgtgagagaa tgaagagaat
     2701 cgaggaggga atcaaggagc ttggatctca aatcttgaag gagcacccag ttgagaacac
     2761 ccagcttcag aacgagaagt tgtaccttta ctaccttcag aacggaagag atatgtacgt
     2821 tgatcaggag cttgacatca acagactttc tgattacgat gttgatcaca tcgttccaca
     2881 gtctttcttg aaggatgatt ctatcgataa caaggttctt acccgttctg ataagaacag
     2941 aggaaagtct gataacgttc catctgagga ggttgttaag aagatgaaga actactggag
     3001 acagcttctt aacgctaagt tgatcaccca gagaaagttc gataacctta ccaaggctga
     3061 gagaggagga ctttctgagc ttgataaggc tggattcatc aagagacagc ttgttgagac
     3121 cagacagatc accaagcacg ttgctcagat ccttgattct cgtatgaaca ccaagtacga
     3181 tgagaacgat aagttgatca gagaggttaa ggttatcacc ttgaagtcta agttggtttc
     3241 tgatttcaga aaggatttcc agttctacaa ggttagagag atcaacaact accaccacgc
     3301 tcacgatgct taccttaacg ctgttgttgg aaccgctctt atcaagaagt acccaaagtt
     3361 ggagtctgag ttcgtttacg gagattacaa ggtttacgat gttagaaaga tgatcgctaa
     3421 gtctgagcag gagatcggaa aggctaccgc taagtacttc ttctactcta acatcatgaa
     3481 cttcttcaag accgagatca cccttgctaa cggagagatc agaaagagac cacttatcga
     3541 gaccaacgga gagaccggag agatcgtttg ggataaggga agagatttcg ctaccgttag
     3601 aaaggttctt tctatgccac aggttaacat cgttaagaaa accgaggttc agaccggagg
     3661 attctctaag gagtctatcc ttccaaagag aaactctgat aagttgatcg ctagaaagaa
     3721 ggattgggac ccaaagaagt acggaggatt cgattctcca accgttgctt actctgttct
     3781 tgttgttgct aaggttgaga agggaaagtc taagaagttg aagtctgtta aggagcttct
     3841 tggaatcacc atcatggagc gttcttcttt cgagaagaac ccaatcgatt tccttgaggc
     3901 taagggatac aaggaggtta agaaggatct tatcatcaag ttgccaaagt actctctttt
     3961 cgagcttgag aacggaagaa agagaatgct tgcttctgct ggagagcttc agaagggaaa
     4021 cgagcttgct cttccatcta agtacgttaa cttcctttac cttgcttctc actacgagaa
     4081 gttgaaggga tctccagagg ataacgagca gaagcagctt ttcgttgagc agcacaagca
     4141 ctaccttgat gagatcatcg agcaaatctc tgagttctct aagagagtta tccttgctga
     4201 tgctaacctt gataaggttc tttctgctta caacaagcac agagataagc caatcagaga
     4261 gcaggctgag aacatcatcc accttttcac ccttaccaac cttggtgctc cagctgcttt
     4321 caagtacttc gataccacca tcgatagaaa aagatacacc tctaccaagg aggttcttga
     4381 tgctaccctt atccaccagt ctatcaccgg actttacgag accagaatcg atctttctca
     4441 gcttggagga gataagagac cagctgctac caagaaggct ggacaggcta agaagaagaa
     4501 gtgagacgtc cgatcgttca aacatttggc aataaagttt cttaagattg aatcctgttg
     4561 ccggtcttgc gatgattatc atataatttc tgttgaatta cgttaagcat gtaataatta
     4621 acatgtaatg catgacgtta tttatgagat gggtttttat gattagagtc ccgcaattat
     4681 acatttaata cgcgatagaa aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg
     4741 cggtgtcatc tatgttacta gatcgggaat tgatcccccc tcgacagctt ccggaaaggg
     4801 cgaattcgca actttgtata caaaagttgc cgagctcgcg gccgcatgtt gttgttacca
     4861 gaaagtaaat aaatgttcaa tctctgatgt tctcaagtaa gtgagtttta ttgggaataa
     4921 tattaactta tgttcttctt gcatttgatt tctttgccgc tctcttcttc tatcttaaat
     4981 ctgtgtatac tatttcacta ttgggctttt tattagtcta taatgggact caaaataagg
     5041 ctttggccca catcaaaaag ataagtcaca aatcaaaact aaattcagag tcttttctcc
     5101 cacatcggtc actgtactca ttttgtgttt gtttatatat tacacgaacc gatctttggt
     5161 acggtactga aggttagcga cgagttttag agctagaaat agcaagttaa aataaggcta
     5221 gtccgttatc aacttgaaaa agtggcaccg agtcggtgct ttttttctag acccagcttt
     5281 cttgtacaaa gttggcatta ccatggcttc gttgaacaac ggaaactcga cttgccttcc
     5341 gcacaataca tcatttcttc ttagcttttt ttcttcttct tcgttcatac agtttttttt
     5401 tgtttatcag cttacatttt cttgaaccgt agctttcgtt ttcttctttt taactttcca
     5461 ttcggagttt ttgtatcttg tttcatagtt tgtcccagga ttagaatgat taggcatcga
     5521 accttcaaga atttgattga ataaaacatc ttcattctta agatatgaag ataatcttca
     5581 aaaggcccct gggaatctga aagaagagaa gcaggcccat ttatatggga aagaacaata
     5641 gtatttctta tataggccca tttaagttga aaacaatctt caaaagtccc acatcgctta
     5701 gataagaaaa cgaagctgag tttatataca gctagagtcg aagtagtgat tgaatattga
     5761 tggggcatct gggttttaga gctagaaata gcaagttaaa ataaggctag tccgttatca
     5821 acttgaaaaa gtggcaccga gtcggtgctt tttttctaga cccagctttc ttgtacaaag
     5881 ttggcattac tgcaggtcga cgacccagct ttcttgtaca aagtggttcg ataattctta
     5941 attaactagt tctagagcgg ccgccaccgc ggtggagctc gaatttcccc gatcgttcaa
     6001 acatttggca ataaagtttc ttaagattga atcctgttgc cggtcttgcg atgattatca
     6061 tataatttct gttgaattac gttaagcatg taataattaa catgtaatgc atgacgttat
     6121 ttatgagatg ggtttttatg attagagtcc cgcaattata catttaatac gcgatagaaa
     6181 acaaaatata gcgcgcaaac taggataaat tatcgcgcgc ggtgtcatct atgttactag
     6241 atcgggaatt cgtaatcatg gtcatagctg tttcctgcct agtcggagcg gccgcgtccg
     6301 tctagctgat agtgacctta ggcgactttt gaacgcgcaa taatggtttc tgacgtatgt
     6361 gcttagctca ttaaactcca gaaacccgcg gctgagtggc tccttcaacg ttgcggttct
     6421 gtcagttcca aacgtaaaac ggcttgtccc gcgtcatcgg cgggggtcat aacgtgactc
     6481 ccttaattct ccgctcatga tcagattgtc gtttcccgcc ttcagtttaa actatcagtg
     6541 tttgacagga tatattggcg ggtaaaccta agagaaaaga gcgtttatta gaataatcgg
     6601 atatttaaaa gggcgtgaaa aggtttatcc gttcgtccat ttgtatgtgc atgccaacca
     6661 cagggttccc cagatctggc gccggccagc gagacgagca agattggccg ccgcccgaaa
     6721 cgatccgaca gcgcgcccag cacaggtgcg caggcaaatt gcaccaacgc atacagcgcc
     6781 agcagaatgc catagtgggc ggtgacgtcg ttcgagtgaa ccagatcgcg caggaggccc
     6841 ggcagcaccg gcataatcag gccgatgccg acagcgtcga gcgcgacagt gctcagaatt
     6901 acgatcaggg gtatgttggg tttcacgtct ggcctccgga ccagcctccg ctggtccgat
     6961 tgaacgcgcg gattctttat cactgataag ttggtggaca tattatgttt atcagtgata
     7021 aagtgtcaag catgacaaag ttgcagccga atacagtgat ccgtgccgcc ctggacctgt
     7081 tgaacgaggt cggcgtagac ggtctgacga cacgcaaact ggcggaacgg ttgggggttc
     7141 agcagccggc gctttactgg cacttcagga acaagcgggc gctgctcgac gcactggccg
     7201 aagccatgct ggcggagaat catacgcatt cggtgccgag agccgacgac gactggcgct
     7261 catttctgat cgggaatgcc cgcagcttca ggcaggcgct gctcgcctac cgcgatggcg
     7321 cgcgcatcca tgccggcacg cgaccgggcg caccgcagat ggaaacggcc gacgcgcagc
     7381 ttcgcttcct ctgcgaggcg ggtttttcgg ccggggacgc cgtcaatgcg ctgatgacaa
     7441 tcagctactt cactgttggg gccgtgcttg aggagcaggc cggcgacagc gatgccggcg
     7501 agcgcggcgg caccgttgaa caggctccgc tctcgccgct gttgcgggcc gcgatagacg
     7561 ccttcgacga agccggtccg gacgcagcgt tcgagcaggg actcgcggtg attgtcgatg
     7621 gattggcgaa aaggaggctc gttgtcagga acgttgaagg accgagaaag ggtgacgatt
     7681 gatcaggacc gctgccggag cgcaacccac tcactacagc agagccatgt agacaacatc
     7741 ccctccccct ttccaccgcg tcagacgccc gtagcagccc gctacgggct ttttcatgcc
     7801 ctgccctagc gtccaagcct cacggccgcg ctcggcctct ctggcggcct tctggcgctc
     7861 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     7921 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     7981 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     8041 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     8101 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     8161 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     8221 cgtggcgctt ttccgctgca taaccctgct tcggggtcat tatagcgatt ttttcggtat
     8281 atccatcctt tttcgcacga tatacaggat tttgccaaag ggttcgtgta gactttcctt
     8341 ggtgtatcca acggcgtcag ccgggcagga taggtgaagt aggcccaccc gcgagcgggt
     8401 gttccttctt cactgtccct tattcgcacc tggcggtgct caacgggaat cctgctctgc
     8461 gaggctggcc ggctaccgcc ggcgtaacag atgagggcaa gcggatggct gatgaaacca
     8521 agccaaccag gaagggcagc ccacctatca aggtgtactg ccttccagac gaacgaagag
     8581 cgattgagga aaaggcggcg gcggccggca tgagcctgtc ggcctacctg ctggccgtcg
     8641 gccagggcta caaaatcacg ggcgtcgtgg actatgagca cgtccgcgag ctggcccgca
     8701 tcaatggcga cctgggccgc ctgggcggcc tgctgaaact ctggctcacc gacgacccgc
     8761 gcacggcgcg gttcggtgat gccacgatcc tcgccctgct ggcgaagatc gaagagaagc
     8821 aggacgagct tggcaaggtc atgatgggcg tggtccgccc gagggcagag ccatgacttt
     8881 tttagccgct aaaacggccg gggggtgcgc gtgattgcca agcacgtccc catgcgctcc
     8941 atcaagaaga gcgacttcgc ggagctggtg aagtacatca ccgacgagca aggcaagacc
     9001 gagcgccttt ccgacgctca ccgggctggt tgccctcgcc gctgggctgg cggccgtcta
     9061 tggccctgca aacgcgccag aaacgccgtc gaagccgtgt gcgagacacc gcggccggcc
     9121 gccggcgttg tggatacctc gcggaaaact tggccctcac tgacagatga ggggcggacg
     9181 ttgacacttg aggggccgac tcacccggcg cggcgttgac agatgagggg caggctcgat
     9241 ttcggccggc gacgtggagc tggccagcct cgcaaatcgg cgaaaacgcc tgattttacg
     9301 cgagtttccc acagatgatg tggacaagcc tggggataag tgccctgcgg tattgacact
     9361 tgaggggcgc gactactgac agatgagggg cgcgatcctt gacacttgag gggcagagtg
     9421 ctgacagatg aggggcgcac ctattgacat ttgaggggct gtccacaggc agaaaatcca
     9481 gcatttgcaa gggtttccgc ccgtttttcg gccaccgcta acctgtcttt taacctgctt
     9541 ttaaaccaat atttataaac cttgttttta accagggctg cgccctgtgc gcgtgaccgc
     9601 gcacgccgaa ggggggtgcc cccccttctc gaaccctccc ggcccgctaa cgcgggcctc
     9661 ccatcccccc aggggctgcg cccctcggcc gcgaacggcc tcaccccaaa aatggcagcg
     9721 ctggcagtcc ttgccattgc cgggatcggg gcagtaacgg gatgggcgat cagcccgagc
     9781 gcgacgcccg gaagcattga cgtgccgcag gtgctggcat cgacattcag cgaccaggtg
     9841 ccgggcagtg agggcggcgg cctgggtggc ggcctgccct tcacttcggc cgtcggggca
     9901 ttcacggact tcatggcggg gccggcaatt tttaccttgg gcattcttgg catagtggtc
     9961 gcgggtgccg tgctcgtgtt cgggggtgcg ataaacccag cgaaccattt gaggtgatag
    10021 gtaagattat accgaggtat gaaaacgaga attggacctt tacagaatta ctctatgaag
    10081 cgccatattt aaaaagctac caagacgaag aggatgaaga ggatgaggag gcagattgcc
    10141 ttgaatatat tgacaatact gataagataa tatatctttt atatagaaga tatcgccgta
    10201 tgtaaggatt tcagggggca aggcataggc agcgcgctta tcaatatatc tatagaatgg
    10261 gcaaagcata aaaacttgca tggactaatg cttgaaaccc aggacaataa ccttatagct
    10321 tgtaaattct atcataattg ggtaatgact ccaacttatt gatagtgttt tatgttcaga
    10381 taatgcccga tgactttgtc atgcagctcc accgattttg agaacgacag cgacttccgt
    10441 cccagccgtg ccaggtgctg cctcagattc aggttatgcc gctcaattcg ctgcgtatat
    10501 cgcttgctga ttacgtgcag ctttcccttc aggcgggatt catacagcgg ccagccatcc
    10561 gtcatccata tcaccacgtc aaagggtgac agcaggctca taagacgccc cagcgtcgcc
    10621 atagtgcgtt caccgaatac gtgcgcaaca accgtcttcc ggagactgtc atacgcgtaa
    10681 aacagccagc gctggcgcga tttagccccg acatagcccc actgttcgtc catttccgcg
    10741 cagacgatga cgtcactgcc cggctgtatg cgcgaggtta ccgactgcgg cctgagtttt
    10801 ttaagtgacg taaaatcgtg ttgaggccaa cgcccataat gcgggctgtt gcccggcatc
    10861 caacgccatt catggccata tcaatgattt tctggtgcgt accgggttga gaagcggtgt
    10921 aagtgaactg cagttgccat gttttacggc agtgagagca gagatagcgc tgatgtccgg
    10981 cggtgctttt gccgttacgc accaccccgt cagtagctga acaggaggga cagctgatag
    11041 aaacagaagc cactggagca cctcaaaaac accatcatac actaaatcag taagttggca
    11101 gcatcaccca taattgtggt ttcaaaatcg gctccgtcga tactatgtta tacgccaact
    11161 ttgaaaacaa ctttgaaaaa gctgttttct ggtatttaag gttttagaat gcaaggaaca
    11221 gtgaattgga gttcgtcttg ttataattag cttcttgggg tatctttaaa tactgtagaa
    11281 aagaggaagg aaataataaa tggctaaaat gagaatatca ccggaattga aaaaactgat
    11341 cgaaaaatac cgctgcgtaa aagatacgga aggaatgtct cctgctaagg tatataagct
    11401 ggtgggagaa aatgaaaacc tatatttaaa aatgacggac agccggtata aagggaccac
    11461 ctatgatgtg gaacgggaaa aggacatgat gctatggctg gaaggaaagc tgcctgttcc
    11521 aaaggtcctg cactttgaac ggcatgatgg ctggagcaat ctgctcatga gtgaggccga
    11581 tggcgtcctt tgctcggaag agtatgaaga tgaacaaagc cctgaaaaga ttatcgagct
    11641 gtatgcggag tgcatcaggc tctttcactc catcgacata tcggattgtc cctatacgaa
    11701 tagcttagac agccgcttag ccgaattgga ttacttactg aataacgatc tggccgatgt
    11761 ggattgcgaa aactgggaag aagacactcc atttaaagat ccgcgcgagc tgtatgattt
    11821 tttaaagacg gaaaagcccg aagaggaact tgtcttttcc cacggcgacc tgggagacag
    11881 caacatcttt gtgaaagatg gcaaagtaag tggctttatt gatcttggga gaagcggcag
    11941 ggcggacaag tggtatgaca ttgccttctg cgtccggtcg atcagggagg atatcgggga
    12001 agaacagtat gtcgagctat tttttgactt actggggatc aagcctgatt gggagaaaat
    12061 aaaatattat attttactgg atgaattgtt ttagtaccta gatgtggcgc aacgatgccg
    12121 gcgacaagca ggagcgcacc gacttcttcc gcatcaagtg ttttggctct caggccgagg
    12181 cccacggcaa gtatttgggc aaggggtcgc tggtattcgt gcagggcaag attcggaata
    12241 ccaagtacga gaaggacggc cagacggtct acgggaccga cttcattgcc gataaggtgg
    12301 attatctgga caccaaggca ccaggcgggt caaatcagga ataagggcac attgccccgg
    12361 cgtgagtcgg ggcaatcccg caaggagggt gaatgaatcg gacgtttgac cggaaggcat
    12421 acaggcaaga actgatcgac gcggggtttt ccgccgagga tgccgaaacc atcgcaagcc
    12481 gcaccgtcat gcgtgcgccc cgcgaaacct tccagtccgt cggctcgatg gtccagcaag
    12541 ctacggccaa gatcgagcgc gacagcgtgc aactggctcc ccctgccctg cccgcgccat
    12601 cggccgccgt ggagcgttcg cgtcgtctcg aacaggaggc ggcaggtttg gcgaagtcga
    12661 tgaccatcga cacgcgagga actatgacga ccaagaagcg aaaaaccgcc ggcgaggacc
    12721 tggcaaaaca ggtcagcgag gccaagcagg ccgcgttgct gaaacacacg aagcagcaga
    12781 tcaaggaaat gcagctttcc ttgttcgata ttgcgccgtg gccggacacg atgcgagcga
    12841 tgccaaacga cacggcccgc tctgccctgt tcaccacgcg caacaagaaa atcccgcgcg
    12901 aggcgctgca aaacaaggtc attttccacg tcaacaagga cgtgaagatc acctacaccg
    12961 gcgtcgagct gcgggccgac gatgacgaac tggtgtggca gcaggtgttg gagtacgcga
    13021 agcgcacccc tatcggcgag ccgatcacct tcacgttcta cgagctttgc caggacctgg
    13081 gctggtcgat caatggccgg tattacacga aggccgagga atgcctgtcg cgcctacagg
    13141 cgacggcgat gggcttcacg tccgaccgcg ttgggcacct ggaatcggtg tcgctgctgc
    13201 accgcttccg cgtcctggac cgtggcaaga aaacgtcccg ttgccaggtc ctgatcgacg
    13261 aggaaatcgt cgtgctgttt gctggcgacc actacacgaa attcatatgg gagaagtacc
    13321 gcaagctgtc gccgacggcc cgacggatgt tcgactattt cagctcgcac cgggagccgt
    13381 acccgctcaa gctggaaacc ttccgcctca tgtgcggatc ggattccacc cgcgtgaaga
    13441 agtggcgcga gcaggtcggc gaagcctgcg aagagttgcg aggcagcggc ctggtggaac
    13501 acgcctgggt caatgatgac ctggtgcatt gcaaacgcta gggccttgtg gggtcagttc
    13561 cggctggggg ttcagcagcc agcgctttac tggcatttca ggaacaagcg ggcactgctc
    13621 gacgcacttg cttcgctcag tatcgctcgg gacgcacggc gcgctctacg aactgccgat
    13681 aaacagagga ttaaaattga caattgtgat taaggctcag attcgacggc ttggagcggc
    13741 cgacgtgcag gatttccgcg agatccgatt gtcggccctg aagaaagctc cagagatgtt
    13801 cgggtccgtt tacgagcacg aggagaaaaa gcccatggag gcgttcgctg aacggttgcg
    13861 agatgccgtg gcattcggcg cctacatcga cggcgagatc attgggctgt cggtcttcaa
    13921 acaggaggac ggccccaagg acgctcacaa ggcgcatctg tccggcgttt tcgtggagcc
    13981 cgaacagcga ggccgagggg tcgccggtat gctgctgcgg gcgttgccgg cgggtttatt
    14041 gctcgtgatg atcgtccgac agattccaac gggaatctgg tggatgcgca tcttcatcct
    14101 cggcgcactt aatatttcgc tattctggag cttgttgttt atttcggtct accgcctgcc
    14161 gggcggggtc gcggcgacgg taggcgctgt gcagccgctg atggtcgtgt tcatctctgc
    14221 cgctctgcta ggtagcccga tacgattgat ggcggtcctg ggggctattt gcggaactgc
    14281 gggcgtggcg ctgttggtgt tgacaccaaa cgcagcgcta gatcctgtcg gcgtcgcagc
    14341 gggcctggcg ggggcggttt ccatggcgtt cggaaccgtg ctgacccgca agtggcaacc
    14401 tcccgtgcct ctgctcacct ttaccgcctg gcaactggcg gccggaggac ttctgctcgt
    14461 tccagtagct ttagtgtttg atccgccaat cccgatgcct acaggaacca atgttctcgg
    14521 cctggcgtgg ctcggcctga tcggagcggg tttaacctac ttcctttggt tccgggggat
    14581 ctcgcgactc gaacctacag ttgtttcctt actgggcttt ctcagcccca gatctggggt
    14641 cgatcagccg gggatgcatc aggccgacag tcggaacttc gggtccccga cctgtaccat
    14701 tcggtgagca atggataggg gagttgatat cgtcaacgtt cacttctaaa gaaatagcgc
    14761 cactcagctt cctcagcggc tttatccagc gatttcctat tatgtcggca tagttctcaa
    14821 gatcgacagc ctgtcacggt taagcgagaa atgaataaga aggctgataa ttcggatctc
    14881 tgcgagggag atgatatttg atcacaggca gcaacgctct gtcatcgtta caatcaacat
    14941 gctaccctcc gcgagatcat ccgtgtttca aacccggcag cttagttgcc gttcttccga
    15001 atagcatcgg taacatgagc aaagtctgcc gccttacaac ggctctcccg ctgacgccgt
    15061 cccggactga tgggctgcct gtatcgagtg gtgattttgt gccgagctgc cggtcgggga
    15121 gctgttggct ggctggtggc aggatatatt gtggtgtaaa caaattgacg cttagacaac
    15181 ttaataacac attgcggacg tttttaatgt actggggtgg tttttctttt caccagtgag
    15241 acgggcaaca gctgattgcc cttcaccgcc tggccctgag agagttgcag caagcggtcc
    15301 acgctggttt gccccagcag gcgaaaatcc tgtttgatgg tggttccgaa atcggcaaaa
    15361 tcccttataa atcaaaagaa tagcccgaga tagggttgag tgttgttcca gtttggaaca
    15421 agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg
    15481 gcgatggccc actacgtgaa ccatcaccca aatcaagttt tttggggtcg aggtgccgta
    15541 aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg
    15601 cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgccatt caggctgcgc
    15661 aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg
    15721 ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt
    15781 aaaacgacgg ccagtgaatt aattcccgat ctagtaacat agatgacacc gcgcgcgata
    15841 atttatccta gtttgcgcgc tatattttgt tttctatcgc gtattaaatg tataattgcg
    15901 ggactctaat cataaaaacc catctcataa ataacgtcat gcattacatg ttaattatta
    15961 catgcttaac gtaattcaac agaaattata tgataatcat cgcaagaccg gcaacaggat
    16021 tcaatcttaa gaaactttat tgccaaatgt ttgaacgatc ggggaaattc gagctcggta
    16081 ccccctcctg ggcacggtcg tacgcgatcc gccgcggttc cacctcacca ttgcgggtat
    16141 gccagttgag ttcgatgagt tcccgtacca gctccaactc gggcgatgcc acgcgcactc
    16201 cgttcagatc tcggtgacgg gcaggaccgg acggggcggt accggcaggc tgaagtccag
    16261 ctgccagaaa cccacgtcat gccagttccc gtgcttgaag ccggccgccc gcagcatgcc
    16321 gcggggggca tatccgagcg cctcgtgcat gcgcacgctc gggtcgttgg gcagcccgat
    16381 gacagcgacc acgctcttga agccctgtgc ctccagggac ttcagcaggt gggtgtagag
    16441 cgtggagccc agtcccgtcc gctggtggcg gggggagacg tacacggtcg actcggccgt
    16501 ccagtcgtag gcgttgcgtg ccttccaggg gcccgcgtag gcgatgccgg cgacctcgcc
    16561 gtccacctcg gcgacgagcc agggatagcg ctcccgcaga cggacgaggt cgtccgtcca
    16621 ctcctgcggt tcctgcggct cggtacggaa gttgaccgtg cttgtctcga tgtagtggtt
    16681 gacgatggtg cagaccgccg gcatgtccgc ctcggtggca cggcggatgt cggccgggcg
    16741 tcgttctggg ctcatggatc ctctagagtc ccccgtgttc tctccaaatg aaatgaactt
    16801 ccttatatag aggaagggtc ttgcgaagga tagtgggatt gtgcgtcatc ccttacgtca
    16861 gtggagatat cacatcaatc cacttgcttt gaagacgtgg ttggaacgtc ttctttttcc
    16921 acgatgctcc tcgtgggtgg gggtccatct ttgggaccac tgtcggcaga ggcatcttca
    16981 acgatggcct ttcctttatc gcaatgatgg catttgtagg agccaccttc cttttccact
    17041 atcttcacaa taaagtgaca gatagctggg caatggaatc cgaggaggtt tccggatatt
    17101 accctttgtt gaaaagtctc aattgccctt tggtcttctg agactgtatc tttgatattt
    17161 ttggagtaga caagtgtgtc gtgctccacc atgttgacga agattttctt cttgtcattg
    17221 agtcgtaaga gactctgtat gaactgttcg ccagtcttta cggcgagttc tgttaggtcc
    17281 tctatttgaa tctttgactc catggccttt gattcagtgg gaactacctt tttagagact
    17341 ccaatctcta ttacttgcct tggtttgtga agcaagcctt gaatcgtcca tactggaata
    17401 gtacttctga tcttgagaaa tatatctttc tctgtgttct tgatgcagtt agtcctgaat
    17461 cttttgactg catctttaac cttcttggga aggtatttga tttcctggag attattgctc
    17521 gggtagatcg tcttgatgag acctgctgcg taagcctctc taaccatctg tgggttagca
    17581 ttctttctga aattgaaaag gctaatctgg ggacctgcag gggcaatacc gccagggttt
    17641 tcccagtcac gacgttgtaa aacgacggcc agtgccaagc tttcgacgag tcagtaataa
    17701 acggcgtcaa agtggttgca gccggcacac acgagtcgtg tttatcaact caaagcacaa
    17761 atacttttcc tcaacctaaa aataaggcaa ttagccaaaa acaactttgc gtgtaaacaa
    17821 cgctcaatac acgtgtcatt ttattattag ctattgcttc accgccttag ctttctcgtg
    17881 acctagtcgt cctcgtcttt tcttcttctt cttctataaa acaataccca aagagctctt
    17941 cttcttcaca attcagattt caatttctca aaatcttaaa aactttctct caattctctc
    18001 taccgtgatc aaggtaaatt tctgtgttcc ttattctctc aaaatcttcg attttgtttt
    18061 cgttcgatcc caatttcgta tatgttcttt ggtttagatt ctgttaatct tagatcgaag
    18121 acgattttct gggtttgatc gttagatatc atcttaattc tcgattaggg tttcatagat
    18181 atcatccgat ttgttcaaat aatttgagtt ttgtcgaata attactcttc gatttgtgat
    18241 ttctatctag atctggtgtt agtttctagt ttgtgcgatc gaatttgtag attaatctga
    18301 gtttttctga ttaacagggt accgggcccc ccctcga
//
