LOCUS       sequence-202761-       18989 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CRISPR/Cas9 2xplex gRNA targeting Medtr8g060730 (Fbl1-like). Also 
            expresses Cas9 from Gmubi promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 18989)
  AUTHORS   Curtin SJ, Tiffin P, Guhlin J, Trujillo DI, Burghart LT, Atkins P, 
            Baltes NJ, Denny R, Voytas DF, Stupar RM, Young ND
  TITLE     Validating Genome-Wide Association Candidates Controlling 
            Quantitative Variation in Nodulation.
  JOURNAL   Plant Physiol. 2017 Feb;173(2):921-931. doi: 10.1104/pp.16.01923. 
            Epub 2017 Jan 5.
  PUBMED    28057894
REFERENCE   2  (bases 1 to 18989)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1104/pp.16.01923"; journalName: "Plant Physiol"; date: 
            "2017-02"; volume: "173"; issue: "2"; pages: "921-931"
COMMENT     Sequence Label: pSC33
FEATURES             Location/Qualifiers
     source          1..18989
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(92..463)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            496..605
                     /note="incP origin of transfer"
     primer_bind     828..847
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1081..1098
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(1286..1936)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    2401..2425
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     protein_bind    3633..3657
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3679..3726
                     /codon_start=1
                     /product="two tandem FLAG(R) epitope tags"
                     /label=2xFLAG
                     /translation="DYKDDDDKDYKDDDDK"
     CDS             3733..3753
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(3778..5214,5404..8067)
                     /codon_start=1
                     /product="Cas9 endonuclease from the Streptococcus pyogenes
                     Type II CRISPR/Cas system"
                     /label=pcoCas9
                     /note="plant codon-optimized Cas9 gene containing the 
                     potato IV2 intron"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     intron          5215..5403
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
     CDS             8068..8115
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     terminator      8126..8378
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    8423..8443
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     misc_RNA        8798..8873
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    complement(8885..8909)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_RNA        9387..9462
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    complement(9474..9498)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(9517..9541)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      9579..10286
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     promoter        10836..11181
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     11124..11144
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             11202..11753
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     terminator      11897..12149
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(12162..12179)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(12162..12178)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12171..12193)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(12318..12337)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     12528..12549
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    12796..12820
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(14416..15564)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(15863..16657)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  16849..17616
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      18239..18954
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 cagctccgcg aagtcgctct tcttgatgga gcgcatgggg acgtgcttgg caatcacgcg
       61 caccccccgg ccgttttagc ggctaaaaaa gtcatggctc tgccctcggg cggaccacgc
      121 ccatcatgac cttgccaagc tcgtcctgct tctcttcgat cttcgccagc agggcgagga
      181 tcgtggcatc accgaaccgc gccgtgcgcg ggtcgtcggt gagccagagt ttcagcaggc
      241 cgcccaggcg gcccaggtcg ccattgatgc gggccagctc gcggacgtgc tcatagtcca
      301 cgacgcccgt gattttgtag ccctggccga cggccagcag gtaggccgac aggctcatgc
      361 cggccgccgc cgccttttcc tcaatcgctc ttcgttcgtc tggaaggcag tacaccttga
      421 taggtgggct gcccttcctg gttggcttgg tttcatcagc catccgcttg ccctcatctg
      481 ttacgccggc ggtagccggc cagcctcgca gagcaggatt cccgttgagc accgccaggt
      541 gcgaataagg gacagtgaag aaggaacacc cgctcgcggg tgggcctact tcacctatcc
      601 tgcccggctg acgccgttgg atacaccaag gaaagtctac acgaaccctt tggcaaaatc
      661 ctgtatatcg tgcgaaaaag gatggatata ccgaaaaaat cgctataatg accccgaagc
      721 agggttatgc agcggaaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
      781 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
      841 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
      901 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
      961 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     1021 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     1081 agcgagtcag tgagcgagga agcggaagag cgccagaagg ccgccagaga ggccgagcgc
     1141 ggccgtgagg cttggacgct agggcagggc atgaaaaagc ccgtagcggg ctgctacggg
     1201 cgtctgacgc ggtggaaagg gggaggggat gttgtctaca tggctctgct gtagtgagtg
     1261 ggttgcgctc cggcagcggt cctgatcaat cgtcaccctt tctcggtcct tcaacgttcc
     1321 tgacaacgag cctccttttc gccaatccat cgacaatcac cgcgagtccc tgctcgaacg
     1381 ctgcgtccgg accggcttcg tcgaaggcgt ctatcgcggc ccgcaacagc ggcgagagcg
     1441 gagcctgttc aacggtgccg ccgcgctcgc cggcatcgct gtcgccggcc tgctcctcaa
     1501 gcacggcccc aacagtgaag tagctgattg tcatcagcgc attgacggcg tccccggccg
     1561 aaaaacccgc ctcgcagagg aagcgaagct gcgcgtcggc cgtttccatc tgcggtgcgc
     1621 ccggtcgcgt gccggcatgg atgcgcgcgc catcgcggta ggcgagcagc gcctgcctga
     1681 agctgcgggc attcccgatc agaaatgagc gccagtcgtc gtcggctctc ggcaccgaat
     1741 gcgtatgatt ctccgccagc atggcttcgg ccagtgcgtc gagcagcgcc cgcttgttcc
     1801 tgaagtgcca gtaaagcgcc ggctgctgaa cccccaaccg ttccgccagt ttgcgtgtcg
     1861 tcagaccgtc tacgccgacc tcgttcaaca ggtccagggc ggcacggatc actgtattcg
     1921 gctgcaactt tgtcatgctt gacactttat cactgataaa cataatatgt ccaccaactt
     1981 atcagtgata aagaatccgc gcgttcaatc ggaccagcgg aggctggtcc ggaggccaga
     2041 cgtgaaaccc aacatacccc tgatcgtaat tctgagcact gtcgcgctcg acgctgtcgg
     2101 catcggcctg attatgccgg tgctgccggg cctcctgcgc gatctggttc actcgaacga
     2161 cgtcaccgcc cactatggca ttctgctggc gctgtatgcg ttggtgcaat ttgcctgcgc
     2221 acctgtgctg ggcgcgctgt cggatcgttt cgggcggcgg ccaatcttgc tcgtctcgct
     2281 ggccggcgcc agatctgggg aaccctgtgg ttggcatgca catacaaatg gacgaacgga
     2341 taaacctttt cacgcccttt taaatatccg attattctaa taaacgctct tttctcttag
     2401 gtttacccgc caatatatcc tgtcaaacac tgatagttta aactgaaggc gggaaacgac
     2461 aatctgatca tgagcggaga attaagggag tcacgttatg acccccgccg atgacgcggg
     2521 acaagccgtt ttacgtttgg aactgacaga accgcaacgt tgaaggagcc actcagccgc
     2581 gggtttctgg agtttaatga gctaagcaca tacgtcagaa accattattg cgcgttcaaa
     2641 agtcgcctaa ggtcactatc agctagacgg acgcggccgc gggcccaata taacaacgac
     2701 gtcgtaacag ataaagcgaa gcttgaaggt gcatgtgact ccgtcaagat tacgaaaccg
     2761 ccaactacca cgcaaattgc aattctcaat ttcctagaag gactctccga aaatgcatcc
     2821 aataccaaat attacccgtg tcataggcac caagtgacac catacatgaa cacgcgtcac
     2881 aatatgactg gagaagggtt ccacacctta tgctataaaa cgccccacac ccctcctcct
     2941 tccttcgcag ttcaattcca atatattcca ttctctctgt gtatttccct acctctccct
     3001 tcaaggttag tcgatttctt ctgtttttct tcttcgttct ttccatgaat tgtgtatgtt
     3061 ctttgatcaa tacgatgttg atttgattgt gttttgtttg gtttcatcga tcttcaattt
     3121 tcataatcag attcagcttt tattatcttt acaacaacgt ccttaatttg atgattcttt
     3181 aatcgtagat ttgctctaat tagagctttt tcatgtcaga tccctttaca acaagcctta
     3241 attgttgatt cattaatcgt agattagggc ttttttcatt gattacttca gatccgttaa
     3301 acgtaaccat agatcagggc tttttcatga attacttcag atccgttaaa caacagcctt
     3361 attttttata cttctgtggt ttttcaagaa attgttcaga tccgttgaca aaaagcctta
     3421 ttcgttgatt ctatatcgtt tttcgagaga tattgctcag atctgttagc aactgccttg
     3481 tttgttgatt ctattgccgt ggattagggt tttttttcac gagattgctt cagatccgta
     3541 cttaagatta cgtaatggat tttgattctg atttatctgt gattgttgac tcgacaggta
     3601 ccttcaaacc tcgaggcgcg ccaagctatc aaacaagttt gtacaaaaaa gcaggctccg
     3661 aattcgccct tcaccatgga ttacaaggat gatgatgata aggattacaa ggatgatgat
     3721 gataagatgg ctccaaagaa gaagagaaag gttggaatcc acggagttcc agctgctgat
     3781 aagaagtact ctatcggact tgacatcgga accaactctg ttggatgggc tgttatcacc
     3841 gatgagtaca aggttccatc taagaagttc aaggttcttg gaaacaccga tagacactct
     3901 atcaagaaga accttatcgg tgctcttctt ttcgattctg gagagaccgc tgaggctacc
     3961 agattgaaga gaaccgctag aagaagatac accagaagaa agaacagaat ctgctacctt
     4021 caggaaatct tctctaacga gatggctaag gttgatgatt ctttcttcca cagacttgag
     4081 gagtctttcc ttgttgagga ggataagaag cacgagagac acccaatctt cggaaacatc
     4141 gttgatgagg ttgcttacca cgagaagtac ccaaccatct accaccttag aaagaagttg
     4201 gttgattcta ccgataaggc tgatcttaga cttatctacc ttgctcttgc tcacatgatc
     4261 aagttcagag gacacttcct tatcgaggga gaccttaacc cagataactc tgatgttgat
     4321 aagttgttca tccagcttgt tcagacctac aaccagcttt tcgaggagaa cccaatcaac
     4381 gcttctggag ttgatgctaa ggctatcctt tctgctagac tttctaagtc tcgtagactt
     4441 gagaacctta tcgctcagct tccaggagag aagaagaacg gacttttcgg aaaccttatc
     4501 gctctttctc ttggacttac cccaaacttc aagtctaact tcgatcttgc tgaggatgct
     4561 aagttgcagc tttctaagga tacctacgat gatgatcttg ataaccttct tgctcagatc
     4621 ggagatcagt acgctgatct tttccttgct gctaagaacc tttctgatgc tatccttctt
     4681 tctgacatcc ttagagttaa caccgagatc accaaggctc cactttctgc ttctatgatc
     4741 aagagatacg atgagcacca ccaggatctt acccttttga aggctcttgt tagacagcag
     4801 cttccagaga agtacaagga aatcttcttc gatcagtcta agaacggata cgctggatac
     4861 atcgatggag gagcttctca ggaggagttc tacaagttca tcaagccaat ccttgagaag
     4921 atggatggaa ccgaggagct tcttgttaag ttgaacagag aggatcttct tagaaagcag
     4981 agaaccttcg ataacggatc tatcccacac cagatccacc ttggagagct tcacgctatc
     5041 cttcgtagac aggaggattt ctacccattc ttgaaggata acagagagaa gatcgagaag
     5101 atccttacct tcagaatccc atactacgtt ggaccacttg ctagaggaaa ctctcgtttc
     5161 gcttggatga ccagaaagtc tgaggagacc atcacccctt ggaacttcga ggaggtaagt
     5221 ttctgcttct acctttgata tatatataat aattatcatt aattagtagt aatataatat
     5281 ttcaaatatt tttttcaaaa taaaagaatg tagtatatag caattgcttt tctgtagttt
     5341 ataagtgtgt atattttaat ttataacttt tctaatatat gaccaaaatt tgttgatgtg
     5401 caggttgttg ataagggagc ttctgctcag tctttcatcg agagaatgac caacttcgat
     5461 aagaaccttc caaacgagaa ggttcttcca aagcactctc ttctttacga gtacttcacc
     5521 gtttacaacg agcttaccaa ggttaagtac gttaccgagg gaatgagaaa gccagctttc
     5581 ctttctggag agcagaagaa ggctatcgtt gatcttcttt tcaagaccaa cagaaaggtt
     5641 accgttaagc agttgaagga ggattacttc aagaagatcg agtgcttcga ttctgttgaa
     5701 atctctggag ttgaggatag attcaacgct tctcttggaa cctaccacga tcttttgaag
     5761 atcatcaagg ataaggattt ccttgataac gaggagaacg aggacatcct tgaggacatc
     5821 gttcttaccc ttaccctttt cgaggataga gagatgatcg aggagagact caagacctac
     5881 gctcaccttt tcgatgataa ggttatgaag cagttgaaga gaagaagata caccggatgg
     5941 ggtagacttt ctcgtaagtt gatcaacgga atcagagata agcagtctgg aaagaccatc
     6001 cttgatttct tgaagtctga tggattcgct aacagaaact tcatgcagct tatccacgat
     6061 gattctctta ccttcaagga ggacatccag aaggctcagg tttctggaca gggagattct
     6121 cttcacgagc acatcgctaa ccttgctgga tctccagcta tcaagaaggg aatccttcag
     6181 accgttaagg ttgttgatga gcttgttaag gttatgggta gacacaagcc agagaacatc
     6241 gttatcgaga tggctagaga gaaccagacc acccagaagg gacagaagaa ctctcgtgag
     6301 agaatgaaga gaatcgagga gggaatcaag gagcttggat ctcaaatctt gaaggagcac
     6361 ccagttgaga acacccagct tcagaacgag aagttgtacc tttactacct tcagaacgga
     6421 agagatatgt acgttgatca ggagcttgac atcaacagac tttctgatta cgatgttgat
     6481 cacatcgttc cacagtcttt cttgaaggat gattctatcg ataacaaggt tcttacccgt
     6541 tctgataaga acagaggaaa gtctgataac gttccatctg aggaggttgt taagaagatg
     6601 aagaactact ggagacagct tcttaacgct aagttgatca cccagagaaa gttcgataac
     6661 cttaccaagg ctgagagagg aggactttct gagcttgata aggctggatt catcaagaga
     6721 cagcttgttg agaccagaca gatcaccaag cacgttgctc agatccttga ttctcgtatg
     6781 aacaccaagt acgatgagaa cgataagttg atcagagagg ttaaggttat caccttgaag
     6841 tctaagttgg tttctgattt cagaaaggat ttccagttct acaaggttag agagatcaac
     6901 aactaccacc acgctcacga tgcttacctt aacgctgttg ttggaaccgc tcttatcaag
     6961 aagtacccaa agttggagtc tgagttcgtt tacggagatt acaaggttta cgatgttaga
     7021 aagatgatcg ctaagtctga gcaggagatc ggaaaggcta ccgctaagta cttcttctac
     7081 tctaacatca tgaacttctt caagaccgag atcacccttg ctaacggaga gatcagaaag
     7141 agaccactta tcgagaccaa cggagagacc ggagagatcg tttgggataa gggaagagat
     7201 ttcgctaccg ttagaaaggt tctttctatg ccacaggtta acatcgttaa gaaaaccgag
     7261 gttcagaccg gaggattctc taaggagtct atccttccaa agagaaactc tgataagttg
     7321 atcgctagaa agaaggattg ggacccaaag aagtacggag gattcgattc tccaaccgtt
     7381 gcttactctg ttcttgttgt tgctaaggtt gagaagggaa agtctaagaa gttgaagtct
     7441 gttaaggagc ttcttggaat caccatcatg gagcgttctt ctttcgagaa gaacccaatc
     7501 gatttccttg aggctaaggg atacaaggag gttaagaagg atcttatcat caagttgcca
     7561 aagtactctc ttttcgagct tgagaacgga agaaagagaa tgcttgcttc tgctggagag
     7621 cttcagaagg gaaacgagct tgctcttcca tctaagtacg ttaacttcct ttaccttgct
     7681 tctcactacg agaagttgaa gggatctcca gaggataacg agcagaagca gcttttcgtt
     7741 gagcagcaca agcactacct tgatgagatc atcgagcaaa tctctgagtt ctctaagaga
     7801 gttatccttg ctgatgctaa ccttgataag gttctttctg cttacaacaa gcacagagat
     7861 aagccaatca gagagcaggc tgagaacatc atccaccttt tcacccttac caaccttggt
     7921 gctccagctg ctttcaagta cttcgatacc accatcgata gaaaaagata cacctctacc
     7981 aaggaggttc ttgatgctac ccttatccac cagtctatca ccggacttta cgagaccaga
     8041 atcgatcttt ctcagcttgg aggagataag agaccagctg ctaccaagaa ggctggacag
     8101 gctaagaaga agaagtgaga cgtccgatcg ttcaaacatt tggcaataaa gtttcttaag
     8161 attgaatcct gttgccggtc ttgcgatgat tatcatataa tttctgttga attacgttaa
     8221 gcatgtaata attaacatgt aatgcatgac gttatttatg agatgggttt ttatgattag
     8281 agtcccgcaa ttatacattt aatacgcgat agaaaacaaa atatagcgcg caaactagga
     8341 taaattatcg cgcgcggtgt catctatgtt actagatcgg gaattgatcc cccctcgaca
     8401 gcttccggaa agggcgaatt cgcaactttg tatacaaaag ttgccgagct cgcggccgca
     8461 tgttgttgtt accagaaagt aaataaatgt tcaatctctg atgttctcaa gtaagtgagt
     8521 tttattggga ataatattaa cttatgttct tcttgcattt gatttctttg ccgctctctt
     8581 cttctatctt aaatctgtgt atactatttc actattgggc tttttattag tctataatgg
     8641 gactcaaaat aaggctttgg cccacatcaa aaagataagt cacaaatcaa aactaaattc
     8701 agagtctttt ctcccacatc ggtcactgta ctcattttgt gtttgtttat atattacacg
     8761 aaccgatctt tggtacgcaa ctggaagctt ctcgaaggtt ttagagctag aaatagcaag
     8821 ttaaaataag gctagtccgt tatcaacttg aaaaagtggc accgagtcgg tgcttttttt
     8881 ctagacccag ctttcttgta caaagttggc attaccatgg cttcgttgaa caacggaaac
     8941 tcgacttgcc ttccgcacaa tacatcattt cttcttagct ttttttcttc ttcttcgttc
     9001 atacagtttt tttttgttta tcagcttaca ttttcttgaa ccgtagcttt cgttttcttc
     9061 tttttaactt tccattcgga gtttttgtat cttgtttcat agtttgtccc aggattagaa
     9121 tgattaggca tcgaaccttc aagaatttga ttgaataaaa catcttcatt cttaagatat
     9181 gaagataatc ttcaaaaggc ccctgggaat ctgaaagaag agaagcaggc ccatttatat
     9241 gggaaagaac aatagtattt cttatatagg cccatttaag ttgaaaacaa tcttcaaaag
     9301 tcccacatcg cttagataag aaaacgaagc tgagtttata tacagctaga gtcgaagtag
     9361 tgattgagta aacctgcaat attccagttt tagagctaga aatagcaagt taaaataagg
     9421 ctagtccgtt atcaacttga aaaagtggca ccgagtcggt gctttttttc tagacccagc
     9481 tttcttgtac aaagttggca ttactgcagg tcgacgaccc agctttcttg tacaaagtgg
     9541 ttcgataatt cttaattaac tagttctaat ctagagtcct gctttaatga gatatgcgag
     9601 acgcctatga tcgcatgata tttgctttca attctgttgt gcacgttgta aaaaacctga
     9661 gcatgtgtag ctcagatcct taccgccggt ttcggttcat tctaatgaat atatcacccg
     9721 ttactatcgt atttttatga ataatattct ccgttcaatt tactgattgt accctactac
     9781 ttatatgtac aatattaaaa tgaaaacaat atattgtgct gaataggttt atagcgacat
     9841 ctatgataga gcgccacaat aacaaacaat tgcgttttat tattacaaat ccaattttaa
     9901 aaaaagcggc agaaccggtc aaacctaaaa gactgattac ataaatctta ttcaaatttc
     9961 aaaagtgccc caggggctag tatctacgac acaccgagcg gcgaactaat aacgctcact
    10021 gaagggaact ccggttcccc gccggcgcgc atgggtgaga ttccttgaag ttgagtattg
    10081 gccgtccgct ctaccgaaag ttacgggcac cattcaaccc ggtccagcac ggcggccggg
    10141 taaccgactt gctgccccga gaattatgca gcattttttt ggtgtatgtg ggccccaaat
    10201 gaagtgcagg tcaaaccttg acagtgacga caaatcgttg ggcgggtcca gggcgaattt
    10261 tgcgacaaca tgtcgaggct cagcaggacc tgcaggcatg caagctagtg aagaatcgat
    10321 ttgctacgta ttgcccctgc aggtccccag attagccttt tcaatttcag aaagaatgct
    10381 aacccacaga tggttagaga ggcttacgca gcaggtctca tcaagacgat ctacccgagc
    10441 aataatctcc aggaaatcaa ataccttccc aagaaggtta aagatgcagt caaaagattc
    10501 aggactaact gcatcaagaa cacagagaaa gatatatttc tcaagatcag aagtactatt
    10561 ccagtatgga cgattcaagg cttgcttcac aaaccaaggc aagtaataga gattggagtc
    10621 tctaaaaagg tagttcccac tgaatcaaag gccatggagt caaagattca aatagaggac
    10681 ctaacagaac tcgccgtaaa gactggcgaa cagttcatac agagtctctt acgactcaat
    10741 gacaagaaga aaatcttcgt caacatggtg gagcacgaca cacttgtcta ctccaaaaat
    10801 atcaaagata cagtctcaga agaccaaagg gcaattgaga cttttcaaca aagggtaata
    10861 tccggaaacc tcctcggatt ccattgccca gctatctgtc actttattgt gaagatagtg
    10921 gaaaaggaag gtggctccta caaatgccat cattgcgata aaggaaaggc catcgttgaa
    10981 gatgcctctg ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa
    11041 aaagaagacg ttccaaccac gtcttcaaag caagtggatt gatgtgatat ctccactgac
    11101 gtaagggatg acgcacaatc ccactatcct tcgcaagacc cttcctctat ataaggaagt
    11161 tcatttcatt tggagagaac acgggggact ctagaggatc catgagccca gaacgacgcc
    11221 cggccgacat ccgccgtgcc accgaggcgg acatgccggc ggtctgcacc atcgtcaacc
    11281 actacatcga gacaagcacg gtcaacttcc gtaccgagcc gcaggaaccg caggagtgga
    11341 cggacgacct cgtccgtctg cgggagcgct atccctggct cgtcgccgag gtggacggcg
    11401 aggtcgccgg catcgcctac gcgggcccct ggaaggcacg caacgcctac gactggacgg
    11461 ccgagtcgac cgtgtacgtc tccccccgcc accagcggac gggactgggc tccacgctct
    11521 acacccacct gctgaagtcc ctggaggcac agggcttcaa gagcgtggtc gctgtcatcg
    11581 ggctgcccaa cgacccgagc gtgcgcatgc acgaggcgct cggatatgcc ccccgcggca
    11641 tgctgcgggc ggccggcttc aagcacggga actggcatga cgtgggtttc tggcagctgg
    11701 acttcagcct gccggtaccg ccccgtccgg tcctgcccgt caccgagatc tgaacggagt
    11761 gcgcgtggca tcgcccgagt tggagctggt acgggaactc atcgaactca actggcatac
    11821 ccgcaatggt gaggtggaac cgcggcggat cgcgtacgac cgtgcccagg agggggtacc
    11881 gagctcgaat ttccccgatc gttcaaacat ttggcaataa agtttcttaa gattgaatcc
    11941 tgttgccggt cttgcgatga ttatcatata atttctgttg aattacgtta agcatgtaat
    12001 aattaacatg taatgcatga cgttatttat gagatgggtt tttatgatta gagtcccgca
    12061 attatacatt taatacgcga tagaaaacaa aatatagcgc gcaaactagg ataaattatc
    12121 gcgcgcggtg tcatctatgt tactagatcg ggaattaatt cactggccgt cgttttacaa
    12181 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct
    12241 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
    12301 agcctgaatg gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
    12361 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
    12421 gcacctcgac cccaaaaaac ttgatttggg tgatggttca cgtagtgggc catcgccctg
    12481 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
    12541 ccaaactgga acaacactca accctatctc gggctattct tttgatttat aagggatttt
    12601 gccgatttcg gaaccaccat caaacaggat tttcgcctgc tggggcaaac cagcgtggac
    12661 cgcttgctgc aactctctca gggccaggcg gtgaagggca atcagctgtt gcccgtctca
    12721 ctggtgaaaa gaaaaaccac cccagtacat taaaaacgtc cgcaatgtgt tattaagttg
    12781 tctaagcgtc aatttgttta caccacaata tatcctgcca ccagccagcc aacagctccc
    12841 cgaccggcag ctcggcacaa aatcaccact cgatacaggc agcccatcag tccgggacgg
    12901 cgtcagcggg agagccgttg taaggcggca gactttgctc atgttaccga tgctattcgg
    12961 aagaacggca actaagctgc cgggtttgaa acacggatga tctcgcggag ggtagcatgt
    13021 tgattgtaac gatgacagag cgttgctgcc tgtgatcaaa tatcatctcc ctcgcagaga
    13081 tccgaattat cagccttctt attcatttct cgcttaaccg tgacaggctg tcgatcttga
    13141 gaactatgcc gacataatag gaaatcgctg gataaagccg ctgaggaagc tgagtggcgc
    13201 tatttcttta gaagtgaacg ttgacgatat caactcccct atccattgct caccgaatgg
    13261 tacaggtcgg ggacccgaag ttccgactgt cggcctgatg catccccggc tgatcgaccc
    13321 cagatctggg gctgagaaag cccagtaagg aaacaactgt aggttcgagt cgcgagatcc
    13381 cccggaacca aaggaagtag gttaaacccg ctccgatcag gccgagccac gccaggccga
    13441 gaacattggt tcctgtaggc atcgggattg gcggatcaaa cactaaagct actggaacga
    13501 gcagaagtcc tccggccgcc agttgccagg cggtaaaggt gagcagaggc acgggaggtt
    13561 gccacttgcg ggtcagcacg gttccgaacg ccatggaaac cgcccccgcc aggcccgctg
    13621 cgacgccgac aggatctagc gctgcgtttg gtgtcaacac caacagcgcc acgcccgcag
    13681 ttccgcaaat agcccccagg accgccatca atcgtatcgg gctacctagc agagcggcag
    13741 agatgaacac gaccatcagc ggctgcacag cgcctaccgt cgccgcgacc ccgcccggca
    13801 ggcggtagac cgaaataaac aacaagctcc agaatagcga aatattaagt gcgccgagga
    13861 tgaagatgcg catccaccag attcccgttg gaatctgtcg gacgatcatc acgagcaata
    13921 aacccgccgg caacgcccgc agcagcatac cggcgacccc tcggcctcgc tgttcgggct
    13981 ccacgaaaac gccggacaga tgcgccttgt gagcgtcctt ggggccgtcc tcctgtttga
    14041 agaccgacag cccaatgatc tcgccgtcga tgtaggcgcc gaatgccacg gcatctcgca
    14101 accgttcagc gaacgcctcc atgggctttt tctcctcgtg ctcgtaaacg gacccgaaca
    14161 tctctggagc tttcttcagg gccgacaatc ggatctcgcg gaaatcctgc acgtcggccg
    14221 ctccaagccg tcgaatctga gccttaatca caattgtcaa ttttaatcct ctgtttatcg
    14281 gcagttcgta gagcgcgccg tgcgtcccga gcgatactga gcgaagcaag tgcgtcgagc
    14341 agtgcccgct tgttcctgaa atgccagtaa agcgctggct gctgaacccc cagccggaac
    14401 tgaccccaca aggccctagc gtttgcaatg caccaggtca tcattgaccc aggcgtgttc
    14461 caccaggccg ctgcctcgca actcttcgca ggcttcgccg acctgctcgc gccacttctt
    14521 cacgcgggtg gaatccgatc cgcacatgag gcggaaggtt tccagcttga gcgggtacgg
    14581 ctcccggtgc gagctgaaat agtcgaacat ccgtcgggcc gtcggcgaca gcttgcggta
    14641 cttctcccat atgaatttcg tgtagtggtc gccagcaaac agcacgacga tttcctcgtc
    14701 gatcaggacc tggcaacggg acgttttctt gccacggtcc aggacgcgga agcggtgcag
    14761 cagcgacacc gattccaggt gcccaacgcg gtcggacgtg aagcccatcg ccgtcgcctg
    14821 taggcgcgac aggcattcct cggccttcgt gtaataccgg ccattgatcg accagcccag
    14881 gtcctggcaa agctcgtaga acgtgaaggt gatcggctcg ccgatagggg tgcgcttcgc
    14941 gtactccaac acctgctgcc acaccagttc gtcatcgtcg gcccgcagct cgacgccggt
    15001 gtaggtgatc ttcacgtcct tgttgacgtg gaaaatgacc ttgttttgca gcgcctcgcg
    15061 cgggattttc ttgttgcgcg tggtgaacag ggcagagcgg gccgtgtcgt ttggcatcgc
    15121 tcgcatcgtg tccggccacg gcgcaatatc gaacaaggaa agctgcattt ccttgatctg
    15181 ctgcttcgtg tgtttcagca acgcggcctg cttggcctcg ctgacctgtt ttgccaggtc
    15241 ctcgccggcg gtttttcgct tcttggtcgt catagttcct cgcgtgtcga tggtcatcga
    15301 cttcgccaaa cctgccgcct cctgttcgag acgacgcgaa cgctccacgg cggccgatgg
    15361 cgcgggcagg gcagggggag ccagttgcac gctgtcgcgc tcgatcttgg ccgtagcttg
    15421 ctggaccatc gagccgacgg actggaaggt ttcgcggggc gcacgcatga cggtgcggct
    15481 tgcgatggtt tcggcatcct cggcggaaaa ccccgcgtcg atcagttctt gcctgtatgc
    15541 cttccggtca aacgtccgat tcattcaccc tccttgcggg attgccccga ctcacgccgg
    15601 ggcaatgtgc ccttattcct gatttgaccc gcctggtgcc ttggtgtcca gataatccac
    15661 cttatcggca atgaagtcgg tcccgtagac cgtctggccg tccttctcgt acttggtatt
    15721 ccgaatcttg ccctgcacga ataccagcga ccccttgccc aaatacttgc cgtgggcctc
    15781 ggcctgagag ccaaaacact tgatgcggaa gaagtcggtg cgctcctgct tgtcgccggc
    15841 atcgttgcgc cacatctagg tactaaaaca attcatccag taaaatataa tattttattt
    15901 tctcccaatc aggcttgatc cccagtaagt caaaaaatag ctcgacatac tgttcttccc
    15961 cgatatcctc cctgatcgac cggacgcaga aggcaatgtc ataccacttg tccgccctgc
    16021 cgcttctccc aagatcaata aagccactta ctttgccatc tttcacaaag atgttgctgt
    16081 ctcccaggtc gccgtgggaa aagacaagtt cctcttcggg cttttccgtc tttaaaaaat
    16141 catacagctc gcgcggatct ttaaatggag tgtcttcttc ccagttttcg caatccacat
    16201 cggccagatc gttattcagt aagtaatcca attcggctaa gcggctgtct aagctattcg
    16261 tatagggaca atccgatatg tcgatggagt gaaagagcct gatgcactcc gcatacagct
    16321 cgataatctt ttcagggctt tgttcatctt catactcttc cgagcaaagg acgccatcgg
    16381 cctcactcat gagcagattg ctccagccat catgccgttc aaagtgcagg acctttggaa
    16441 caggcagctt tccttccagc catagcatca tgtccttttc ccgttccaca tcataggtgg
    16501 tccctttata ccggctgtcc gtcattttta aatataggtt ttcattttct cccaccagct
    16561 tatatacctt agcaggagac attccttccg tatcttttac gcagcggtat ttttcgatca
    16621 gttttttcaa ttccggtgat attctcattt tagccattta ttatttcctt cctcttttct
    16681 acagtattta aagatacccc aagaagctaa ttataacaag acgaactcca attcactgtt
    16741 ccttgcattc taaaacctta aataccagaa aacagctttt tcaaagttgt tttcaaagtt
    16801 ggcgtataac atagtatcga cggagccgat tttgaaacca caattatggg tgatgctgcc
    16861 aacttactga tttagtgtat gatggtgttt ttgaggtgct ccagtggctt ctgtttctat
    16921 cagctgtccc tcctgttcag ctactgacgg ggtggtgcgt aacggcaaaa gcaccgccgg
    16981 acatcagcgc tatctctgct ctcactgccg taaaacatgg caactgcagt tcacttacac
    17041 cgcttctcaa cccggtacgc accagaaaat cattgatatg gccatgaatg gcgttggatg
    17101 ccgggcaaca gcccgcatta tgggcgttgg cctcaacacg attttacgtc acttaaaaaa
    17161 ctcaggccgc agtcggtaac ctcgcgcata cagccgggca gtgacgtcat cgtctgcgcg
    17221 gaaatggacg aacagtgggg ctatgtcggg gctaaatcgc gccagcgctg gctgttttac
    17281 gcgtatgaca gtctccggaa gacggttgtt gcgcacgtat tcggtgaacg cactatggcg
    17341 acgctggggc gtcttatgag cctgctgtca ccctttgacg tggtgatatg gatgacggat
    17401 ggctggccgc tgtatgaatc ccgcctgaag ggaaagctgc acgtaatcag caagcgatat
    17461 acgcagcgaa ttgagcggca taacctgaat ctgaggcagc acctggcacg gctgggacgg
    17521 aagtcgctgt cgttctcaaa atcggtggag ctgcatgaca aagtcatcgg gcattatctg
    17581 aacataaaac actatcaata agttggagtc attacccaat tatgatagaa tttacaagct
    17641 ataaggttat tgtcctgggt ttcaagcatt agtccatgca agtttttatg ctttgcccat
    17701 tctatagata tattgataag cgcgctgcct atgccttgcc ccctgaaatc cttacatacg
    17761 gcgatatctt ctatataaaa gatatattat cttatcagta ttgtcaatat attcaaggca
    17821 atctgcctcc tcatcctctt catcctcttc gtcttggtag ctttttaaat atggcgcttc
    17881 atagagtaat tctgtaaagg tccaattctc gttttcatac ctcggtataa tcttacctat
    17941 cacctcaaat ggttcgctgg gtttatcgca cccccgaaca cgagcacggc acccgcgacc
    18001 actatgccaa gaatgcccaa ggtaaaaatt gccggccccg ccatgaagtc cgtgaatgcc
    18061 ccgacggccg aagtgaaggg caggccgcca cccaggccgc cgccctcact gcccggcacc
    18121 tggtcgctga atgtcgatgc cagcacctgc ggcacgtcaa tgcttccggg cgtcgcgctc
    18181 gggctgatcg cccatcccgt tactgccccg atcccggcaa tggcaaggac tgccagcgct
    18241 gccatttttg gggtgaggcc gttcgcggcc gaggggcgca gcccctgggg ggatgggagg
    18301 cccgcgttag cgggccggga gggttcgaga agggggggca ccccccttcg gcgtgcgcgg
    18361 tcacgcgcac agggcgcagc cctggttaaa aacaaggttt ataaatattg gtttaaaagc
    18421 aggttaaaag acaggttagc ggtggccgaa aaacgggcgg aaacccttgc aaatgctgga
    18481 ttttctgcct gtggacagcc cctcaaatgt caataggtgc gcccctcatc tgtcagcact
    18541 ctgcccctca agtgtcaagg atcgcgcccc tcatctgtca gtagtcgcgc ccctcaagtg
    18601 tcaataccgc agggcactta tccccaggct tgtccacatc atctgtggga aactcgcgta
    18661 aaatcaggcg ttttcgccga tttgcgaggc tggccagctc cacgtcgccg gccgaaatcg
    18721 agcctgcccc tcatctgtca acgccgcgcc gggtgagtcg gcccctcaag tgtcaacgtc
    18781 cgcccctcat ctgtcagtga gggccaagtt ttccgcgagg tatccacaac gccggcggcc
    18841 ggccgcggtg tctcgcacac ggcttcgacg gcgtttctgg cgcgtttgca gggccataga
    18901 cggccgccag cccagcggcg agggcaacca gcccggtgag cgtcggaaag gcgctcggtc
    18961 ttgccttgct cgtcggtgat gtacttcac
//
