LOCUS       sequence-202764-       18988 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CRISPR/Cas9 2xplex gRNA targeting Medtr3g107390 (MtRdr6). Also 
            expresses Cas9 from Gmubi promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 18988)
  AUTHORS   Shaun Curtin
  TITLE     Shaun Curtin CRISPR plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 18988)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pSC37
FEATURES             Location/Qualifiers
     source          1..18988
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(116..487)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            520..629
                     /note="incP origin of transfer"
     primer_bind     852..871
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1105..1122
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(1310..1960)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    2425..2449
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     protein_bind    3657..3681
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3703..3750
                     /codon_start=1
                     /product="two tandem FLAG(R) epitope tags"
                     /label=2xFLAG
                     /translation="DYKDDDDKDYKDDDDK"
     CDS             3757..3777
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(3802..5238,5428..8091)
                     /codon_start=1
                     /product="Cas9 endonuclease from the Streptococcus pyogenes
                     Type II CRISPR/Cas system"
                     /label=pcoCas9
                     /note="plant codon-optimized Cas9 gene containing the 
                     potato IV2 intron"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     intron          5239..5427
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
     CDS             8092..8139
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     terminator      8150..8402
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    8447..8467
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     misc_RNA        8822..8897
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    complement(8909..8933)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_RNA        9410..9485
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    complement(9497..9521)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(9540..9564)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      9602..10309
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     promoter        10859..11204
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     11147..11167
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             11225..11776
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     terminator      11920..12172
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(12185..12202)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(12185..12201)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12194..12216)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(12341..12360)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     12551..12572
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    12819..12843
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(14439..15587)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(15886..16680)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  16872..17639
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      18332..18967
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 ttgctcgtcg gtgatgtact tcaccagctc cgcgaagtcg ctcttcttga tggagcgcat
       61 ggggacgtgc ttggcaatca cgcgcacccc ccggccgttt tagcggctaa aaaagtcatg
      121 gctctgccct cgggcggacc acgcccatca tgaccttgcc aagctcgtcc tgcttctctt
      181 cgatcttcgc cagcagggcg aggatcgtgg catcaccgaa ccgcgccgtg cgcgggtcgt
      241 cggtgagcca gagtttcagc aggccgccca ggcggcccag gtcgccattg atgcgggcca
      301 gctcgcggac gtgctcatag tccacgacgc ccgtgatttt gtagccctgg ccgacggcca
      361 gcaggtaggc cgacaggctc atgccggccg ccgccgcctt ttcctcaatc gctcttcgtt
      421 cgtctggaag gcagtacacc ttgataggtg ggctgccctt cctggttggc ttggtttcat
      481 cagccatccg cttgccctca tctgttacgc cggcggtagc cggccagcct cgcagagcag
      541 gattcccgtt gagcaccgcc aggtgcgaat aagggacagt gaagaaggaa cacccgctcg
      601 cgggtgggcc tacttcacct atcctgcccg gctgacgccg ttggatacac caaggaaagt
      661 ctacacgaac cctttggcaa aatcctgtat atcgtgcgaa aaaggatgga tataccgaaa
      721 aaatcgctat aatgaccccg aagcagggtt atgcagcgga aaagcgccac gcttcccgaa
      781 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
      841 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
      901 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
      961 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     1021 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     1081 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccag
     1141 aaggccgcca gagaggccga gcgcggccgt gaggcttgga cgctagggca gggcatgaaa
     1201 aagcccgtag cgggctgcta cgggcgtctg acgcggtgga aagggggagg ggatgttgtc
     1261 tacatggctc tgctgtagtg agtgggttgc gctccggcag cggtcctgat caatcgtcac
     1321 cctttctcgg tccttcaacg ttcctgacaa cgagcctcct tttcgccaat ccatcgacaa
     1381 tcaccgcgag tccctgctcg aacgctgcgt ccggaccggc ttcgtcgaag gcgtctatcg
     1441 cggcccgcaa cagcggcgag agcggagcct gttcaacggt gccgccgcgc tcgccggcat
     1501 cgctgtcgcc ggcctgctcc tcaagcacgg ccccaacagt gaagtagctg attgtcatca
     1561 gcgcattgac ggcgtccccg gccgaaaaac ccgcctcgca gaggaagcga agctgcgcgt
     1621 cggccgtttc catctgcggt gcgcccggtc gcgtgccggc atggatgcgc gcgccatcgc
     1681 ggtaggcgag cagcgcctgc ctgaagctgc gggcattccc gatcagaaat gagcgccagt
     1741 cgtcgtcggc tctcggcacc gaatgcgtat gattctccgc cagcatggct tcggccagtg
     1801 cgtcgagcag cgcccgcttg ttcctgaagt gccagtaaag cgccggctgc tgaaccccca
     1861 accgttccgc cagtttgcgt gtcgtcagac cgtctacgcc gacctcgttc aacaggtcca
     1921 gggcggcacg gatcactgta ttcggctgca actttgtcat gcttgacact ttatcactga
     1981 taaacataat atgtccacca acttatcagt gataaagaat ccgcgcgttc aatcggacca
     2041 gcggaggctg gtccggaggc cagacgtgaa acccaacata cccctgatcg taattctgag
     2101 cactgtcgcg ctcgacgctg tcggcatcgg cctgattatg ccggtgctgc cgggcctcct
     2161 gcgcgatctg gttcactcga acgacgtcac cgcccactat ggcattctgc tggcgctgta
     2221 tgcgttggtg caatttgcct gcgcacctgt gctgggcgcg ctgtcggatc gtttcgggcg
     2281 gcggccaatc ttgctcgtct cgctggccgg cgccagatct ggggaaccct gtggttggca
     2341 tgcacataca aatggacgaa cggataaacc ttttcacgcc cttttaaata tccgattatt
     2401 ctaataaacg ctcttttctc ttaggtttac ccgccaatat atcctgtcaa acactgatag
     2461 tttaaactga aggcgggaaa cgacaatctg atcatgagcg gagaattaag ggagtcacgt
     2521 tatgaccccc gccgatgacg cgggacaagc cgttttacgt ttggaactga cagaaccgca
     2581 acgttgaagg agccactcag ccgcgggttt ctggagttta atgagctaag cacatacgtc
     2641 agaaaccatt attgcgcgtt caaaagtcgc ctaaggtcac tatcagctag acggacgcgg
     2701 ccgcgggccc aatataacaa cgacgtcgta acagataaag cgaagcttga aggtgcatgt
     2761 gactccgtca agattacgaa accgccaact accacgcaaa ttgcaattct caatttccta
     2821 gaaggactct ccgaaaatgc atccaatacc aaatattacc cgtgtcatag gcaccaagtg
     2881 acaccataca tgaacacgcg tcacaatatg actggagaag ggttccacac cttatgctat
     2941 aaaacgcccc acacccctcc tccttccttc gcagttcaat tccaatatat tccattctct
     3001 ctgtgtattt ccctacctct cccttcaagg ttagtcgatt tcttctgttt ttcttcttcg
     3061 ttctttccat gaattgtgta tgttctttga tcaatacgat gttgatttga ttgtgttttg
     3121 tttggtttca tcgatcttca attttcataa tcagattcag cttttattat ctttacaaca
     3181 acgtccttaa tttgatgatt ctttaatcgt agatttgctc taattagagc tttttcatgt
     3241 cagatccctt tacaacaagc cttaattgtt gattcattaa tcgtagatta gggctttttt
     3301 cattgattac ttcagatccg ttaaacgtaa ccatagatca gggctttttc atgaattact
     3361 tcagatccgt taaacaacag ccttattttt tatacttctg tggtttttca agaaattgtt
     3421 cagatccgtt gacaaaaagc cttattcgtt gattctatat cgtttttcga gagatattgc
     3481 tcagatctgt tagcaactgc cttgtttgtt gattctattg ccgtggatta gggttttttt
     3541 tcacgagatt gcttcagatc cgtacttaag attacgtaat ggattttgat tctgatttat
     3601 ctgtgattgt tgactcgaca ggtaccttca aacctcgagg cgcgccaagc tatcaaacaa
     3661 gtttgtacaa aaaagcaggc tccgaattcg cccttcacca tggattacaa ggatgatgat
     3721 gataaggatt acaaggatga tgatgataag atggctccaa agaagaagag aaaggttgga
     3781 atccacggag ttccagctgc tgataagaag tactctatcg gacttgacat cggaaccaac
     3841 tctgttggat gggctgttat caccgatgag tacaaggttc catctaagaa gttcaaggtt
     3901 cttggaaaca ccgatagaca ctctatcaag aagaacctta tcggtgctct tcttttcgat
     3961 tctggagaga ccgctgaggc taccagattg aagagaaccg ctagaagaag atacaccaga
     4021 agaaagaaca gaatctgcta ccttcaggaa atcttctcta acgagatggc taaggttgat
     4081 gattctttct tccacagact tgaggagtct ttccttgttg aggaggataa gaagcacgag
     4141 agacacccaa tcttcggaaa catcgttgat gaggttgctt accacgagaa gtacccaacc
     4201 atctaccacc ttagaaagaa gttggttgat tctaccgata aggctgatct tagacttatc
     4261 taccttgctc ttgctcacat gatcaagttc agaggacact tccttatcga gggagacctt
     4321 aacccagata actctgatgt tgataagttg ttcatccagc ttgttcagac ctacaaccag
     4381 cttttcgagg agaacccaat caacgcttct ggagttgatg ctaaggctat cctttctgct
     4441 agactttcta agtctcgtag acttgagaac cttatcgctc agcttccagg agagaagaag
     4501 aacggacttt tcggaaacct tatcgctctt tctcttggac ttaccccaaa cttcaagtct
     4561 aacttcgatc ttgctgagga tgctaagttg cagctttcta aggataccta cgatgatgat
     4621 cttgataacc ttcttgctca gatcggagat cagtacgctg atcttttcct tgctgctaag
     4681 aacctttctg atgctatcct tctttctgac atccttagag ttaacaccga gatcaccaag
     4741 gctccacttt ctgcttctat gatcaagaga tacgatgagc accaccagga tcttaccctt
     4801 ttgaaggctc ttgttagaca gcagcttcca gagaagtaca aggaaatctt cttcgatcag
     4861 tctaagaacg gatacgctgg atacatcgat ggaggagctt ctcaggagga gttctacaag
     4921 ttcatcaagc caatccttga gaagatggat ggaaccgagg agcttcttgt taagttgaac
     4981 agagaggatc ttcttagaaa gcagagaacc ttcgataacg gatctatccc acaccagatc
     5041 caccttggag agcttcacgc tatccttcgt agacaggagg atttctaccc attcttgaag
     5101 gataacagag agaagatcga gaagatcctt accttcagaa tcccatacta cgttggacca
     5161 cttgctagag gaaactctcg tttcgcttgg atgaccagaa agtctgagga gaccatcacc
     5221 ccttggaact tcgaggaggt aagtttctgc ttctaccttt gatatatata taataattat
     5281 cattaattag tagtaatata atatttcaaa tatttttttc aaaataaaag aatgtagtat
     5341 atagcaattg cttttctgta gtttataagt gtgtatattt taatttataa cttttctaat
     5401 atatgaccaa aatttgttga tgtgcaggtt gttgataagg gagcttctgc tcagtctttc
     5461 atcgagagaa tgaccaactt cgataagaac cttccaaacg agaaggttct tccaaagcac
     5521 tctcttcttt acgagtactt caccgtttac aacgagctta ccaaggttaa gtacgttacc
     5581 gagggaatga gaaagccagc tttcctttct ggagagcaga agaaggctat cgttgatctt
     5641 cttttcaaga ccaacagaaa ggttaccgtt aagcagttga aggaggatta cttcaagaag
     5701 atcgagtgct tcgattctgt tgaaatctct ggagttgagg atagattcaa cgcttctctt
     5761 ggaacctacc acgatctttt gaagatcatc aaggataagg atttccttga taacgaggag
     5821 aacgaggaca tccttgagga catcgttctt acccttaccc ttttcgagga tagagagatg
     5881 atcgaggaga gactcaagac ctacgctcac cttttcgatg ataaggttat gaagcagttg
     5941 aagagaagaa gatacaccgg atggggtaga ctttctcgta agttgatcaa cggaatcaga
     6001 gataagcagt ctggaaagac catccttgat ttcttgaagt ctgatggatt cgctaacaga
     6061 aacttcatgc agcttatcca cgatgattct cttaccttca aggaggacat ccagaaggct
     6121 caggtttctg gacagggaga ttctcttcac gagcacatcg ctaaccttgc tggatctcca
     6181 gctatcaaga agggaatcct tcagaccgtt aaggttgttg atgagcttgt taaggttatg
     6241 ggtagacaca agccagagaa catcgttatc gagatggcta gagagaacca gaccacccag
     6301 aagggacaga agaactctcg tgagagaatg aagagaatcg aggagggaat caaggagctt
     6361 ggatctcaaa tcttgaagga gcacccagtt gagaacaccc agcttcagaa cgagaagttg
     6421 tacctttact accttcagaa cggaagagat atgtacgttg atcaggagct tgacatcaac
     6481 agactttctg attacgatgt tgatcacatc gttccacagt ctttcttgaa ggatgattct
     6541 atcgataaca aggttcttac ccgttctgat aagaacagag gaaagtctga taacgttcca
     6601 tctgaggagg ttgttaagaa gatgaagaac tactggagac agcttcttaa cgctaagttg
     6661 atcacccaga gaaagttcga taaccttacc aaggctgaga gaggaggact ttctgagctt
     6721 gataaggctg gattcatcaa gagacagctt gttgagacca gacagatcac caagcacgtt
     6781 gctcagatcc ttgattctcg tatgaacacc aagtacgatg agaacgataa gttgatcaga
     6841 gaggttaagg ttatcacctt gaagtctaag ttggtttctg atttcagaaa ggatttccag
     6901 ttctacaagg ttagagagat caacaactac caccacgctc acgatgctta ccttaacgct
     6961 gttgttggaa ccgctcttat caagaagtac ccaaagttgg agtctgagtt cgtttacgga
     7021 gattacaagg tttacgatgt tagaaagatg atcgctaagt ctgagcagga gatcggaaag
     7081 gctaccgcta agtacttctt ctactctaac atcatgaact tcttcaagac cgagatcacc
     7141 cttgctaacg gagagatcag aaagagacca cttatcgaga ccaacggaga gaccggagag
     7201 atcgtttggg ataagggaag agatttcgct accgttagaa aggttctttc tatgccacag
     7261 gttaacatcg ttaagaaaac cgaggttcag accggaggat tctctaagga gtctatcctt
     7321 ccaaagagaa actctgataa gttgatcgct agaaagaagg attgggaccc aaagaagtac
     7381 ggaggattcg attctccaac cgttgcttac tctgttcttg ttgttgctaa ggttgagaag
     7441 ggaaagtcta agaagttgaa gtctgttaag gagcttcttg gaatcaccat catggagcgt
     7501 tcttctttcg agaagaaccc aatcgatttc cttgaggcta agggatacaa ggaggttaag
     7561 aaggatctta tcatcaagtt gccaaagtac tctcttttcg agcttgagaa cggaagaaag
     7621 agaatgcttg cttctgctgg agagcttcag aagggaaacg agcttgctct tccatctaag
     7681 tacgttaact tcctttacct tgcttctcac tacgagaagt tgaagggatc tccagaggat
     7741 aacgagcaga agcagctttt cgttgagcag cacaagcact accttgatga gatcatcgag
     7801 caaatctctg agttctctaa gagagttatc cttgctgatg ctaaccttga taaggttctt
     7861 tctgcttaca acaagcacag agataagcca atcagagagc aggctgagaa catcatccac
     7921 cttttcaccc ttaccaacct tggtgctcca gctgctttca agtacttcga taccaccatc
     7981 gatagaaaaa gatacacctc taccaaggag gttcttgatg ctacccttat ccaccagtct
     8041 atcaccggac tttacgagac cagaatcgat ctttctcagc ttggaggaga taagagacca
     8101 gctgctacca agaaggctgg acaggctaag aagaagaagt gagacgtccg atcgttcaaa
     8161 catttggcaa taaagtttct taagattgaa tcctgttgcc ggtcttgcga tgattatcat
     8221 ataatttctg ttgaattacg ttaagcatgt aataattaac atgtaatgca tgacgttatt
     8281 tatgagatgg gtttttatga ttagagtccc gcaattatac atttaatacg cgatagaaaa
     8341 caaaatatag cgcgcaaact aggataaatt atcgcgcgcg gtgtcatcta tgttactaga
     8401 tcgggaattg atcccccctc gacagcttcc ggaaagggcg aattcgcaac tttgtataca
     8461 aaagttgccg agctcgcggc cgcatgttgt tgttaccaga aagtaaataa atgttcaatc
     8521 tctgatgttc tcaagtaagt gagttttatt gggaataata ttaacttatg ttcttcttgc
     8581 atttgatttc tttgccgctc tcttcttcta tcttaaatct gtgtatacta tttcactatt
     8641 gggcttttta ttagtctata atgggactca aaataaggct ttggcccaca tcaaaaagat
     8701 aagtcacaaa tcaaaactaa attcagagtc ttttctccca catcggtcac tgtactcatt
     8761 ttgtgtttgt ttatatatta cacgaaccga tctttggtac gtgccaaatt gaacaaggcc
     8821 tgttttagag ctagaaatag caagttaaaa taaggctagt ccgttatcaa cttgaaaaag
     8881 tggcaccgag tcggtgcttt ttttctagac ccagctttct tgtacaaagt tggcattacc
     8941 atggcttcgt tgaacaacgg aaactcgact tgccttccgc acaatacatc atttcttctt
     9001 agcttttttt cttcttcttc gttcatacag tttttttttg tttatcagct tacattttct
     9061 tgaaccgtag ctttcgtttt cttcttttta actttccatt cggagttttt gtatcttgtt
     9121 tcatagtttg tcccaggatt agaatgatta ggcatcgaac cttcaagaat ttgattgaat
     9181 aaaacatctt cattcttaag atatgaagat aatcttcaaa aggcccctgg gaatctgaaa
     9241 gaagagaagc aggcccattt atatgggaaa gaacaatagt atttcttata taggcccatt
     9301 taagttgaaa acaatcttca aaagtcccac atcgcttaga taagaaaacg aagctgagtt
     9361 tatatacagc tagagtcgaa gtagtgattg ataaaatgtg attttaaggg ttttagagct
     9421 agaaatagca agttaaaata aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc
     9481 ggtgcttttt ttctagaccc agctttcttg tacaaagttg gcattactgc aggtcgacga
     9541 cccagctttc ttgtacaaag tggttcgata attcttaatt aactagttct aatctagagt
     9601 cctgctttaa tgagatatgc gagacgccta tgatcgcatg atatttgctt tcaattctgt
     9661 tgtgcacgtt gtaaaaaacc tgagcatgtg tagctcagat ccttaccgcc ggtttcggtt
     9721 cattctaatg aatatatcac ccgttactat cgtattttta tgaataatat tctccgttca
     9781 atttactgat tgtaccctac tacttatatg tacaatatta aaatgaaaac aatatattgt
     9841 gctgaatagg tttatagcga catctatgat agagcgccac aataacaaac aattgcgttt
     9901 tattattaca aatccaattt taaaaaaagc ggcagaaccg gtcaaaccta aaagactgat
     9961 tacataaatc ttattcaaat ttcaaaagtg ccccaggggc tagtatctac gacacaccga
    10021 gcggcgaact aataacgctc actgaaggga actccggttc cccgccggcg cgcatgggtg
    10081 agattccttg aagttgagta ttggccgtcc gctctaccga aagttacggg caccattcaa
    10141 cccggtccag cacggcggcc gggtaaccga cttgctgccc cgagaattat gcagcatttt
    10201 tttggtgtat gtgggcccca aatgaagtgc aggtcaaacc ttgacagtga cgacaaatcg
    10261 ttgggcgggt ccagggcgaa ttttgcgaca acatgtcgag gctcagcagg acctgcaggc
    10321 atgcaagcta gtgaagaatc gatttgctac gtattgcccc tgcaggtccc cagattagcc
    10381 ttttcaattt cagaaagaat gctaacccac agatggttag agaggcttac gcagcaggtc
    10441 tcatcaagac gatctacccg agcaataatc tccaggaaat caaatacctt cccaagaagg
    10501 ttaaagatgc agtcaaaaga ttcaggacta actgcatcaa gaacacagag aaagatatat
    10561 ttctcaagat cagaagtact attccagtat ggacgattca aggcttgctt cacaaaccaa
    10621 ggcaagtaat agagattgga gtctctaaaa aggtagttcc cactgaatca aaggccatgg
    10681 agtcaaagat tcaaatagag gacctaacag aactcgccgt aaagactggc gaacagttca
    10741 tacagagtct cttacgactc aatgacaaga agaaaatctt cgtcaacatg gtggagcacg
    10801 acacacttgt ctactccaaa aatatcaaag atacagtctc agaagaccaa agggcaattg
    10861 agacttttca acaaagggta atatccggaa acctcctcgg attccattgc ccagctatct
    10921 gtcactttat tgtgaagata gtggaaaagg aaggtggctc ctacaaatgc catcattgcg
    10981 ataaaggaaa ggccatcgtt gaagatgcct ctgccgacag tggtcccaaa gatggacccc
    11041 cacccacgag gagcatcgtg gaaaaagaag acgttccaac cacgtcttca aagcaagtgg
    11101 attgatgtga tatctccact gacgtaaggg atgacgcaca atcccactat ccttcgcaag
    11161 acccttcctc tatataagga agttcatttc atttggagag aacacggggg actctagagg
    11221 atccatgagc ccagaacgac gcccggccga catccgccgt gccaccgagg cggacatgcc
    11281 ggcggtctgc accatcgtca accactacat cgagacaagc acggtcaact tccgtaccga
    11341 gccgcaggaa ccgcaggagt ggacggacga cctcgtccgt ctgcgggagc gctatccctg
    11401 gctcgtcgcc gaggtggacg gcgaggtcgc cggcatcgcc tacgcgggcc cctggaaggc
    11461 acgcaacgcc tacgactgga cggccgagtc gaccgtgtac gtctcccccc gccaccagcg
    11521 gacgggactg ggctccacgc tctacaccca cctgctgaag tccctggagg cacagggctt
    11581 caagagcgtg gtcgctgtca tcgggctgcc caacgacccg agcgtgcgca tgcacgaggc
    11641 gctcggatat gccccccgcg gcatgctgcg ggcggccggc ttcaagcacg ggaactggca
    11701 tgacgtgggt ttctggcagc tggacttcag cctgccggta ccgccccgtc cggtcctgcc
    11761 cgtcaccgag atctgaacgg agtgcgcgtg gcatcgcccg agttggagct ggtacgggaa
    11821 ctcatcgaac tcaactggca tacccgcaat ggtgaggtgg aaccgcggcg gatcgcgtac
    11881 gaccgtgccc aggagggggt accgagctcg aatttccccg atcgttcaaa catttggcaa
    11941 taaagtttct taagattgaa tcctgttgcc ggtcttgcga tgattatcat ataatttctg
    12001 ttgaattacg ttaagcatgt aataattaac atgtaatgca tgacgttatt tatgagatgg
    12061 gtttttatga ttagagtccc gcaattatac atttaatacg cgatagaaaa caaaatatag
    12121 cgcgcaaact aggataaatt atcgcgcgcg gtgtcatcta tgttactaga tcgggaatta
    12181 attcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta
    12241 atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg
    12301 atcgcccttc ccaacagttg cgcagcctga atggcgcccg ctcctttcgc tttcttccct
    12361 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
    12421 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgattt gggtgatggt
    12481 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
    12541 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcgggctat
    12601 tcttttgatt tataagggat tttgccgatt tcggaaccac catcaaacag gattttcgcc
    12661 tgctggggca aaccagcgtg gaccgcttgc tgcaactctc tcagggccag gcggtgaagg
    12721 gcaatcagct gttgcccgtc tcactggtga aaagaaaaac caccccagta cattaaaaac
    12781 gtccgcaatg tgttattaag ttgtctaagc gtcaatttgt ttacaccaca atatatcctg
    12841 ccaccagcca gccaacagct ccccgaccgg cagctcggca caaaatcacc actcgataca
    12901 ggcagcccat cagtccggga cggcgtcagc gggagagccg ttgtaaggcg gcagactttg
    12961 ctcatgttac cgatgctatt cggaagaacg gcaactaagc tgccgggttt gaaacacgga
    13021 tgatctcgcg gagggtagca tgttgattgt aacgatgaca gagcgttgct gcctgtgatc
    13081 aaatatcatc tccctcgcag agatccgaat tatcagcctt cttattcatt tctcgcttaa
    13141 ccgtgacagg ctgtcgatct tgagaactat gccgacataa taggaaatcg ctggataaag
    13201 ccgctgagga agctgagtgg cgctatttct ttagaagtga acgttgacga tatcaactcc
    13261 cctatccatt gctcaccgaa tggtacaggt cggggacccg aagttccgac tgtcggcctg
    13321 atgcatcccc ggctgatcga ccccagatct ggggctgaga aagcccagta aggaaacaac
    13381 tgtaggttcg agtcgcgaga tcccccggaa ccaaaggaag taggttaaac ccgctccgat
    13441 caggccgagc cacgccaggc cgagaacatt ggttcctgta ggcatcggga ttggcggatc
    13501 aaacactaaa gctactggaa cgagcagaag tcctccggcc gccagttgcc aggcggtaaa
    13561 ggtgagcaga ggcacgggag gttgccactt gcgggtcagc acggttccga acgccatgga
    13621 aaccgccccc gccaggcccg ctgcgacgcc gacaggatct agcgctgcgt ttggtgtcaa
    13681 caccaacagc gccacgcccg cagttccgca aatagccccc aggaccgcca tcaatcgtat
    13741 cgggctacct agcagagcgg cagagatgaa cacgaccatc agcggctgca cagcgcctac
    13801 cgtcgccgcg accccgcccg gcaggcggta gaccgaaata aacaacaagc tccagaatag
    13861 cgaaatatta agtgcgccga ggatgaagat gcgcatccac cagattcccg ttggaatctg
    13921 tcggacgatc atcacgagca ataaacccgc cggcaacgcc cgcagcagca taccggcgac
    13981 ccctcggcct cgctgttcgg gctccacgaa aacgccggac agatgcgcct tgtgagcgtc
    14041 cttggggccg tcctcctgtt tgaagaccga cagcccaatg atctcgccgt cgatgtaggc
    14101 gccgaatgcc acggcatctc gcaaccgttc agcgaacgcc tccatgggct ttttctcctc
    14161 gtgctcgtaa acggacccga acatctctgg agctttcttc agggccgaca atcggatctc
    14221 gcggaaatcc tgcacgtcgg ccgctccaag ccgtcgaatc tgagccttaa tcacaattgt
    14281 caattttaat cctctgttta tcggcagttc gtagagcgcg ccgtgcgtcc cgagcgatac
    14341 tgagcgaagc aagtgcgtcg agcagtgccc gcttgttcct gaaatgccag taaagcgctg
    14401 gctgctgaac ccccagccgg aactgacccc acaaggccct agcgtttgca atgcaccagg
    14461 tcatcattga cccaggcgtg ttccaccagg ccgctgcctc gcaactcttc gcaggcttcg
    14521 ccgacctgct cgcgccactt cttcacgcgg gtggaatccg atccgcacat gaggcggaag
    14581 gtttccagct tgagcgggta cggctcccgg tgcgagctga aatagtcgaa catccgtcgg
    14641 gccgtcggcg acagcttgcg gtacttctcc catatgaatt tcgtgtagtg gtcgccagca
    14701 aacagcacga cgatttcctc gtcgatcagg acctggcaac gggacgtttt cttgccacgg
    14761 tccaggacgc ggaagcggtg cagcagcgac accgattcca ggtgcccaac gcggtcggac
    14821 gtgaagccca tcgccgtcgc ctgtaggcgc gacaggcatt cctcggcctt cgtgtaatac
    14881 cggccattga tcgaccagcc caggtcctgg caaagctcgt agaacgtgaa ggtgatcggc
    14941 tcgccgatag gggtgcgctt cgcgtactcc aacacctgct gccacaccag ttcgtcatcg
    15001 tcggcccgca gctcgacgcc ggtgtaggtg atcttcacgt ccttgttgac gtggaaaatg
    15061 accttgtttt gcagcgcctc gcgcgggatt ttcttgttgc gcgtggtgaa cagggcagag
    15121 cgggccgtgt cgtttggcat cgctcgcatc gtgtccggcc acggcgcaat atcgaacaag
    15181 gaaagctgca tttccttgat ctgctgcttc gtgtgtttca gcaacgcggc ctgcttggcc
    15241 tcgctgacct gttttgccag gtcctcgccg gcggtttttc gcttcttggt cgtcatagtt
    15301 cctcgcgtgt cgatggtcat cgacttcgcc aaacctgccg cctcctgttc gagacgacgc
    15361 gaacgctcca cggcggccga tggcgcgggc agggcagggg gagccagttg cacgctgtcg
    15421 cgctcgatct tggccgtagc ttgctggacc atcgagccga cggactggaa ggtttcgcgg
    15481 ggcgcacgca tgacggtgcg gcttgcgatg gtttcggcat cctcggcgga aaaccccgcg
    15541 tcgatcagtt cttgcctgta tgccttccgg tcaaacgtcc gattcattca ccctccttgc
    15601 gggattgccc cgactcacgc cggggcaatg tgcccttatt cctgatttga cccgcctggt
    15661 gccttggtgt ccagataatc caccttatcg gcaatgaagt cggtcccgta gaccgtctgg
    15721 ccgtccttct cgtacttggt attccgaatc ttgccctgca cgaataccag cgaccccttg
    15781 cccaaatact tgccgtgggc ctcggcctga gagccaaaac acttgatgcg gaagaagtcg
    15841 gtgcgctcct gcttgtcgcc ggcatcgttg cgccacatct aggtactaaa acaattcatc
    15901 cagtaaaata taatatttta ttttctccca atcaggcttg atccccagta agtcaaaaaa
    15961 tagctcgaca tactgttctt ccccgatatc ctccctgatc gaccggacgc agaaggcaat
    16021 gtcataccac ttgtccgccc tgccgcttct cccaagatca ataaagccac ttactttgcc
    16081 atctttcaca aagatgttgc tgtctcccag gtcgccgtgg gaaaagacaa gttcctcttc
    16141 gggcttttcc gtctttaaaa aatcatacag ctcgcgcgga tctttaaatg gagtgtcttc
    16201 ttcccagttt tcgcaatcca catcggccag atcgttattc agtaagtaat ccaattcggc
    16261 taagcggctg tctaagctat tcgtataggg acaatccgat atgtcgatgg agtgaaagag
    16321 cctgatgcac tccgcataca gctcgataat cttttcaggg ctttgttcat cttcatactc
    16381 ttccgagcaa aggacgccat cggcctcact catgagcaga ttgctccagc catcatgccg
    16441 ttcaaagtgc aggacctttg gaacaggcag ctttccttcc agccatagca tcatgtcctt
    16501 ttcccgttcc acatcatagg tggtcccttt ataccggctg tccgtcattt ttaaatatag
    16561 gttttcattt tctcccacca gcttatatac cttagcagga gacattcctt ccgtatcttt
    16621 tacgcagcgg tatttttcga tcagtttttt caattccggt gatattctca ttttagccat
    16681 ttattatttc cttcctcttt tctacagtat ttaaagatac cccaagaagc taattataac
    16741 aagacgaact ccaattcact gttccttgca ttctaaaacc ttaaatacca gaaaacagct
    16801 ttttcaaagt tgttttcaaa gttggcgtat aacatagtat cgacggagcc gattttgaaa
    16861 ccacaattat gggtgatgct gccaacttac tgatttagtg tatgatggtg tttttgaggt
    16921 gctccagtgg cttctgtttc tatcagctgt ccctcctgtt cagctactga cggggtggtg
    16981 cgtaacggca aaagcaccgc cggacatcag cgctatctct gctctcactg ccgtaaaaca
    17041 tggcaactgc agttcactta caccgcttct caacccggta cgcaccagaa aatcattgat
    17101 atggccatga atggcgttgg atgccgggca acagcccgca ttatgggcgt tggcctcaac
    17161 acgattttac gtcacttaaa aaactcaggc cgcagtcggt aacctcgcgc atacagccgg
    17221 gcagtgacgt catcgtctgc gcggaaatgg acgaacagtg gggctatgtc ggggctaaat
    17281 cgcgccagcg ctggctgttt tacgcgtatg acagtctccg gaagacggtt gttgcgcacg
    17341 tattcggtga acgcactatg gcgacgctgg ggcgtcttat gagcctgctg tcaccctttg
    17401 acgtggtgat atggatgacg gatggctggc cgctgtatga atcccgcctg aagggaaagc
    17461 tgcacgtaat cagcaagcga tatacgcagc gaattgagcg gcataacctg aatctgaggc
    17521 agcacctggc acggctggga cggaagtcgc tgtcgttctc aaaatcggtg gagctgcatg
    17581 acaaagtcat cgggcattat ctgaacataa aacactatca ataagttgga gtcattaccc
    17641 aattatgata gaatttacaa gctataaggt tattgtcctg ggtttcaagc attagtccat
    17701 gcaagttttt atgctttgcc cattctatag atatattgat aagcgcgctg cctatgcctt
    17761 gccccctgaa atccttacat acggcgatat cttctatata aaagatatat tatcttatca
    17821 gtattgtcaa tatattcaag gcaatctgcc tcctcatcct cttcatcctc ttcgtcttgg
    17881 tagcttttta aatatggcgc ttcatagagt aattctgtaa aggtccaatt ctcgttttca
    17941 tacctcggta taatcttacc tatcacctca aatggttcgc tgggtttatc gcacccccga
    18001 acacgagcac ggcacccgcg accactatgc caagaatgcc caaggtaaaa attgccggcc
    18061 ccgccatgaa gtccgtgaat gccccgacgg ccgaagtgaa gggcaggccg ccacccaggc
    18121 cgccgccctc actgcccggc acctggtcgc tgaatgtcga tgccagcacc tgcggcacgt
    18181 caatgcttcc gggcgtcgcg ctcgggctga tcgcccatcc cgttactgcc ccgatcccgg
    18241 caatggcaag gactgccagc gctgccattt ttggggtgag gccgttcgcg gccgaggggc
    18301 gcagcccctg gggggatggg aggcccgcgt tagcgggccg ggagggttcg agaagggggg
    18361 gcacccccct tcggcgtgcg cggtcacgcg cacagggcgc agccctggtt aaaaacaagg
    18421 tttataaata ttggtttaaa agcaggttaa aagacaggtt agcggtggcc gaaaaacggg
    18481 cggaaaccct tgcaaatgct ggattttctg cctgtggaca gcccctcaaa tgtcaatagg
    18541 tgcgcccctc atctgtcagc actctgcccc tcaagtgtca aggatcgcgc ccctcatctg
    18601 tcagtagtcg cgcccctcaa gtgtcaatac cgcagggcac ttatccccag gcttgtccac
    18661 atcatctgtg ggaaactcgc gtaaaatcag gcgttttcgc cgatttgcga ggctggccag
    18721 ctccacgtcg ccggccgaaa tcgagcctgc ccctcatctg tcaacgccgc gccgggtgag
    18781 tcggcccctc aagtgtcaac gtccgcccct catctgtcag tgagggccaa gttttccgcg
    18841 aggtatccac aacgccggcg gccggccgcg gtgtctcgca cacggcttcg acggcgtttc
    18901 tggcgcgttt gcagggccat agacggccgc cagcccagcg gcgagggcaa ccagcccggt
    18961 gagcgtcgga aaggcgctcg gtcttgcc
//
