LOCUS       sequence-202765-       18336 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CRISPR/Cas9 2xplex gRNA targeting Medtr3g107390 (MtRdr6). Also 
            expresses Cas9 from AtUBQ10 promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 18336)
  AUTHORS   Shaun Curtin
  TITLE     Shaun Curtin CRISPR plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 18336)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pSC38
FEATURES             Location/Qualifiers
     source          1..18336
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             59..106
                     /codon_start=1
                     /product="two tandem FLAG(R) epitope tags"
                     /label=2xFLAG
                     /translation="DYKDDDDKDYKDDDDK"
     CDS             113..133
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(158..1594,1784..4447)
                     /codon_start=1
                     /product="Cas9 endonuclease from the Streptococcus pyogenes
                     Type II CRISPR/Cas system"
                     /label=pcoCas9
                     /note="plant codon-optimized Cas9 gene containing the 
                     potato IV2 intron"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     intron          1595..1783
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
     CDS             4448..4495
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     terminator      4506..4758
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    4803..4823
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     misc_RNA        5178..5253
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    complement(5265..5289)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_RNA        5766..5841
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    complement(5853..5877)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5896..5920)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      5984..6236
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(6254..6270)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6254..6270)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     misc_feature    6536..6560
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             7025..7675
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     primer_bind     complement(7863..7880)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(8114..8133)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     oriT            8356..8465
                     /note="incP origin of transfer"
     CDS             8498..8869
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     rep_origin      8996..9711
                     /label=oriV
                     /note="incP origin of replication"
     mobile_element  10334..11101
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     CDS             11293..12087
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     CDS             12386..13534
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     misc_feature    15130..15154
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     primer_bind     complement(15401..15422)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     15613..15632
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     15772..15788
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      complement(15801..16053)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(16197..16748)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(16769..17114)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(16806..16826)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     primer_bind     17650..17666
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ccaagctatc aaacaagttt gtacaaaaaa gcaggctccg aattcgccct tcaccatgga
       61 ttacaaggat gatgatgata aggattacaa ggatgatgat gataagatgg ctccaaagaa
      121 gaagagaaag gttggaatcc acggagttcc agctgctgat aagaagtact ctatcggact
      181 tgacatcgga accaactctg ttggatgggc tgttatcacc gatgagtaca aggttccatc
      241 taagaagttc aaggttcttg gaaacaccga tagacactct atcaagaaga accttatcgg
      301 tgctcttctt ttcgattctg gagagaccgc tgaggctacc agattgaaga gaaccgctag
      361 aagaagatac accagaagaa agaacagaat ctgctacctt caggaaatct tctctaacga
      421 gatggctaag gttgatgatt ctttcttcca cagacttgag gagtctttcc ttgttgagga
      481 ggataagaag cacgagagac acccaatctt cggaaacatc gttgatgagg ttgcttacca
      541 cgagaagtac ccaaccatct accaccttag aaagaagttg gttgattcta ccgataaggc
      601 tgatcttaga cttatctacc ttgctcttgc tcacatgatc aagttcagag gacacttcct
      661 tatcgaggga gaccttaacc cagataactc tgatgttgat aagttgttca tccagcttgt
      721 tcagacctac aaccagcttt tcgaggagaa cccaatcaac gcttctggag ttgatgctaa
      781 ggctatcctt tctgctagac tttctaagtc tcgtagactt gagaacctta tcgctcagct
      841 tccaggagag aagaagaacg gacttttcgg aaaccttatc gctctttctc ttggacttac
      901 cccaaacttc aagtctaact tcgatcttgc tgaggatgct aagttgcagc tttctaagga
      961 tacctacgat gatgatcttg ataaccttct tgctcagatc ggagatcagt acgctgatct
     1021 tttccttgct gctaagaacc tttctgatgc tatccttctt tctgacatcc ttagagttaa
     1081 caccgagatc accaaggctc cactttctgc ttctatgatc aagagatacg atgagcacca
     1141 ccaggatctt acccttttga aggctcttgt tagacagcag cttccagaga agtacaagga
     1201 aatcttcttc gatcagtcta agaacggata cgctggatac atcgatggag gagcttctca
     1261 ggaggagttc tacaagttca tcaagccaat ccttgagaag atggatggaa ccgaggagct
     1321 tcttgttaag ttgaacagag aggatcttct tagaaagcag agaaccttcg ataacggatc
     1381 tatcccacac cagatccacc ttggagagct tcacgctatc cttcgtagac aggaggattt
     1441 ctacccattc ttgaaggata acagagagaa gatcgagaag atccttacct tcagaatccc
     1501 atactacgtt ggaccacttg ctagaggaaa ctctcgtttc gcttggatga ccagaaagtc
     1561 tgaggagacc atcacccctt ggaacttcga ggaggtaagt ttctgcttct acctttgata
     1621 tatatataat aattatcatt aattagtagt aatataatat ttcaaatatt tttttcaaaa
     1681 taaaagaatg tagtatatag caattgcttt tctgtagttt ataagtgtgt atattttaat
     1741 ttataacttt tctaatatat gaccaaaatt tgttgatgtg caggttgttg ataagggagc
     1801 ttctgctcag tctttcatcg agagaatgac caacttcgat aagaaccttc caaacgagaa
     1861 ggttcttcca aagcactctc ttctttacga gtacttcacc gtttacaacg agcttaccaa
     1921 ggttaagtac gttaccgagg gaatgagaaa gccagctttc ctttctggag agcagaagaa
     1981 ggctatcgtt gatcttcttt tcaagaccaa cagaaaggtt accgttaagc agttgaagga
     2041 ggattacttc aagaagatcg agtgcttcga ttctgttgaa atctctggag ttgaggatag
     2101 attcaacgct tctcttggaa cctaccacga tcttttgaag atcatcaagg ataaggattt
     2161 ccttgataac gaggagaacg aggacatcct tgaggacatc gttcttaccc ttaccctttt
     2221 cgaggataga gagatgatcg aggagagact caagacctac gctcaccttt tcgatgataa
     2281 ggttatgaag cagttgaaga gaagaagata caccggatgg ggtagacttt ctcgtaagtt
     2341 gatcaacgga atcagagata agcagtctgg aaagaccatc cttgatttct tgaagtctga
     2401 tggattcgct aacagaaact tcatgcagct tatccacgat gattctctta ccttcaagga
     2461 ggacatccag aaggctcagg tttctggaca gggagattct cttcacgagc acatcgctaa
     2521 ccttgctgga tctccagcta tcaagaaggg aatccttcag accgttaagg ttgttgatga
     2581 gcttgttaag gttatgggta gacacaagcc agagaacatc gttatcgaga tggctagaga
     2641 gaaccagacc acccagaagg gacagaagaa ctctcgtgag agaatgaaga gaatcgagga
     2701 gggaatcaag gagcttggat ctcaaatctt gaaggagcac ccagttgaga acacccagct
     2761 tcagaacgag aagttgtacc tttactacct tcagaacgga agagatatgt acgttgatca
     2821 ggagcttgac atcaacagac tttctgatta cgatgttgat cacatcgttc cacagtcttt
     2881 cttgaaggat gattctatcg ataacaaggt tcttacccgt tctgataaga acagaggaaa
     2941 gtctgataac gttccatctg aggaggttgt taagaagatg aagaactact ggagacagct
     3001 tcttaacgct aagttgatca cccagagaaa gttcgataac cttaccaagg ctgagagagg
     3061 aggactttct gagcttgata aggctggatt catcaagaga cagcttgttg agaccagaca
     3121 gatcaccaag cacgttgctc agatccttga ttctcgtatg aacaccaagt acgatgagaa
     3181 cgataagttg atcagagagg ttaaggttat caccttgaag tctaagttgg tttctgattt
     3241 cagaaaggat ttccagttct acaaggttag agagatcaac aactaccacc acgctcacga
     3301 tgcttacctt aacgctgttg ttggaaccgc tcttatcaag aagtacccaa agttggagtc
     3361 tgagttcgtt tacggagatt acaaggttta cgatgttaga aagatgatcg ctaagtctga
     3421 gcaggagatc ggaaaggcta ccgctaagta cttcttctac tctaacatca tgaacttctt
     3481 caagaccgag atcacccttg ctaacggaga gatcagaaag agaccactta tcgagaccaa
     3541 cggagagacc ggagagatcg tttgggataa gggaagagat ttcgctaccg ttagaaaggt
     3601 tctttctatg ccacaggtta acatcgttaa gaaaaccgag gttcagaccg gaggattctc
     3661 taaggagtct atccttccaa agagaaactc tgataagttg atcgctagaa agaaggattg
     3721 ggacccaaag aagtacggag gattcgattc tccaaccgtt gcttactctg ttcttgttgt
     3781 tgctaaggtt gagaagggaa agtctaagaa gttgaagtct gttaaggagc ttcttggaat
     3841 caccatcatg gagcgttctt ctttcgagaa gaacccaatc gatttccttg aggctaaggg
     3901 atacaaggag gttaagaagg atcttatcat caagttgcca aagtactctc ttttcgagct
     3961 tgagaacgga agaaagagaa tgcttgcttc tgctggagag cttcagaagg gaaacgagct
     4021 tgctcttcca tctaagtacg ttaacttcct ttaccttgct tctcactacg agaagttgaa
     4081 gggatctcca gaggataacg agcagaagca gcttttcgtt gagcagcaca agcactacct
     4141 tgatgagatc atcgagcaaa tctctgagtt ctctaagaga gttatccttg ctgatgctaa
     4201 ccttgataag gttctttctg cttacaacaa gcacagagat aagccaatca gagagcaggc
     4261 tgagaacatc atccaccttt tcacccttac caaccttggt gctccagctg ctttcaagta
     4321 cttcgatacc accatcgata gaaaaagata cacctctacc aaggaggttc ttgatgctac
     4381 ccttatccac cagtctatca ccggacttta cgagaccaga atcgatcttt ctcagcttgg
     4441 aggagataag agaccagctg ctaccaagaa ggctggacag gctaagaaga agaagtgaga
     4501 cgtccgatcg ttcaaacatt tggcaataaa gtttcttaag attgaatcct gttgccggtc
     4561 ttgcgatgat tatcatataa tttctgttga attacgttaa gcatgtaata attaacatgt
     4621 aatgcatgac gttatttatg agatgggttt ttatgattag agtcccgcaa ttatacattt
     4681 aatacgcgat agaaaacaaa atatagcgcg caaactagga taaattatcg cgcgcggtgt
     4741 catctatgtt actagatcgg gaattgatcc cccctcgaca gcttccggaa agggcgaatt
     4801 cgcaactttg tatacaaaag ttgccgagct cgcggccgca tgttgttgtt accagaaagt
     4861 aaataaatgt tcaatctctg atgttctcaa gtaagtgagt tttattggga ataatattaa
     4921 cttatgttct tcttgcattt gatttctttg ccgctctctt cttctatctt aaatctgtgt
     4981 atactatttc actattgggc tttttattag tctataatgg gactcaaaat aaggctttgg
     5041 cccacatcaa aaagataagt cacaaatcaa aactaaattc agagtctttt ctcccacatc
     5101 ggtcactgta ctcattttgt gtttgtttat atattacacg aaccgatctt tggtacgtgc
     5161 caaattgaac aaggcctgtt ttagagctag aaatagcaag ttaaaataag gctagtccgt
     5221 tatcaacttg aaaaagtggc accgagtcgg tgcttttttt ctagacccag ctttcttgta
     5281 caaagttggc attaccatgg cttcgttgaa caacggaaac tcgacttgcc ttccgcacaa
     5341 tacatcattt cttcttagct ttttttcttc ttcttcgttc atacagtttt tttttgttta
     5401 tcagcttaca ttttcttgaa ccgtagcttt cgttttcttc tttttaactt tccattcgga
     5461 gtttttgtat cttgtttcat agtttgtccc aggattagaa tgattaggca tcgaaccttc
     5521 aagaatttga ttgaataaaa catcttcatt cttaagatat gaagataatc ttcaaaaggc
     5581 ccctgggaat ctgaaagaag agaagcaggc ccatttatat gggaaagaac aatagtattt
     5641 cttatatagg cccatttaag ttgaaaacaa tcttcaaaag tcccacatcg cttagataag
     5701 aaaacgaagc tgagtttata tacagctaga gtcgaagtag tgattgataa aatgtgattt
     5761 taagggtttt agagctagaa atagcaagtt aaaataaggc tagtccgtta tcaacttgaa
     5821 aaagtggcac cgagtcggtg ctttttttct agacccagct ttcttgtaca aagttggcat
     5881 tactgcaggt cgacgaccca gctttcttgt acaaagtggt tcgataattc ttaattaact
     5941 agttctagag cggccgccac cgcggtggag ctcgaatttc cccgatcgtt caaacatttg
     6001 gcaataaagt ttcttaagat tgaatcctgt tgccggtctt gcgatgatta tcatataatt
     6061 tctgttgaat tacgttaagc atgtaataat taacatgtaa tgcatgacgt tatttatgag
     6121 atgggttttt atgattagag tcccgcaatt atacatttaa tacgcgatag aaaacaaaat
     6181 atagcgcgca aactaggata aattatcgcg cgcggtgtca tctatgttac tagatcggga
     6241 attcgtaatc atggtcatag ctgtttcctg cctagtcgga gcggccgcgt ccgtctagct
     6301 gatagtgacc ttaggcgact tttgaacgcg caataatggt ttctgacgta tgtgcttagc
     6361 tcattaaact ccagaaaccc gcggctgagt ggctccttca acgttgcggt tctgtcagtt
     6421 ccaaacgtaa aacggcttgt cccgcgtcat cggcgggggt cataacgtga ctcccttaat
     6481 tctccgctca tgatcagatt gtcgtttccc gccttcagtt taaactatca gtgtttgaca
     6541 ggatatattg gcgggtaaac ctaagagaaa agagcgttta ttagaataat cggatattta
     6601 aaagggcgtg aaaaggttta tccgttcgtc catttgtatg tgcatgccaa ccacagggtt
     6661 ccccagatct ggcgccggcc agcgagacga gcaagattgg ccgccgcccg aaacgatccg
     6721 acagcgcgcc cagcacaggt gcgcaggcaa attgcaccaa cgcatacagc gccagcagaa
     6781 tgccatagtg ggcggtgacg tcgttcgagt gaaccagatc gcgcaggagg cccggcagca
     6841 ccggcataat caggccgatg ccgacagcgt cgagcgcgac agtgctcaga attacgatca
     6901 ggggtatgtt gggtttcacg tctggcctcc ggaccagcct ccgctggtcc gattgaacgc
     6961 gcggattctt tatcactgat aagttggtgg acatattatg tttatcagtg ataaagtgtc
     7021 aagcatgaca aagttgcagc cgaatacagt gatccgtgcc gccctggacc tgttgaacga
     7081 ggtcggcgta gacggtctga cgacacgcaa actggcggaa cggttggggg ttcagcagcc
     7141 ggcgctttac tggcacttca ggaacaagcg ggcgctgctc gacgcactgg ccgaagccat
     7201 gctggcggag aatcatacgc attcggtgcc gagagccgac gacgactggc gctcatttct
     7261 gatcgggaat gcccgcagct tcaggcaggc gctgctcgcc taccgcgatg gcgcgcgcat
     7321 ccatgccggc acgcgaccgg gcgcaccgca gatggaaacg gccgacgcgc agcttcgctt
     7381 cctctgcgag gcgggttttt cggccgggga cgccgtcaat gcgctgatga caatcagcta
     7441 cttcactgtt ggggccgtgc ttgaggagca ggccggcgac agcgatgccg gcgagcgcgg
     7501 cggcaccgtt gaacaggctc cgctctcgcc gctgttgcgg gccgcgatag acgccttcga
     7561 cgaagccggt ccggacgcag cgttcgagca gggactcgcg gtgattgtcg atggattggc
     7621 gaaaaggagg ctcgttgtca ggaacgttga aggaccgaga aagggtgacg attgatcagg
     7681 accgctgccg gagcgcaacc cactcactac agcagagcca tgtagacaac atcccctccc
     7741 cctttccacc gcgtcagacg cccgtagcag cccgctacgg gctttttcat gccctgccct
     7801 agcgtccaag cctcacggcc gcgctcggcc tctctggcgg ccttctggcg ctcttccgct
     7861 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     7921 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     7981 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     8041 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     8101 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     8161 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     8221 cttttccgct gcataaccct gcttcggggt cattatagcg attttttcgg tatatccatc
     8281 ctttttcgca cgatatacag gattttgcca aagggttcgt gtagactttc cttggtgtat
     8341 ccaacggcgt cagccgggca ggataggtga agtaggccca cccgcgagcg ggtgttcctt
     8401 cttcactgtc ccttattcgc acctggcggt gctcaacggg aatcctgctc tgcgaggctg
     8461 gccggctacc gccggcgtaa cagatgaggg caagcggatg gctgatgaaa ccaagccaac
     8521 caggaagggc agcccaccta tcaaggtgta ctgccttcca gacgaacgaa gagcgattga
     8581 ggaaaaggcg gcggcggccg gcatgagcct gtcggcctac ctgctggccg tcggccaggg
     8641 ctacaaaatc acgggcgtcg tggactatga gcacgtccgc gagctggccc gcatcaatgg
     8701 cgacctgggc cgcctgggcg gcctgctgaa actctggctc accgacgacc cgcgcacggc
     8761 gcggttcggt gatgccacga tcctcgccct gctggcgaag atcgaagaga agcaggacga
     8821 gcttggcaag gtcatgatgg gcgtggtccg cccgagggca gagccatgac ttttttagcc
     8881 gctaaaacgg ccggggggtg cgcgtgattg ccaagcacgt ccccatgcgc tccatcaaga
     8941 agagcgactt cgcggagctg gtgaagtaca tcaccgacga gcaaggcaag accgagcgcc
     9001 tttccgacgc tcaccgggct ggttgccctc gccgctgggc tggcggccgt ctatggccct
     9061 gcaaacgcgc cagaaacgcc gtcgaagccg tgtgcgagac accgcggccg gccgccggcg
     9121 ttgtggatac ctcgcggaaa acttggccct cactgacaga tgaggggcgg acgttgacac
     9181 ttgaggggcc gactcacccg gcgcggcgtt gacagatgag gggcaggctc gatttcggcc
     9241 ggcgacgtgg agctggccag cctcgcaaat cggcgaaaac gcctgatttt acgcgagttt
     9301 cccacagatg atgtggacaa gcctggggat aagtgccctg cggtattgac acttgagggg
     9361 cgcgactact gacagatgag gggcgcgatc cttgacactt gaggggcaga gtgctgacag
     9421 atgaggggcg cacctattga catttgaggg gctgtccaca ggcagaaaat ccagcatttg
     9481 caagggtttc cgcccgtttt tcggccaccg ctaacctgtc ttttaacctg cttttaaacc
     9541 aatatttata aaccttgttt ttaaccaggg ctgcgccctg tgcgcgtgac cgcgcacgcc
     9601 gaaggggggt gccccccctt ctcgaaccct cccggcccgc taacgcgggc ctcccatccc
     9661 cccaggggct gcgcccctcg gccgcgaacg gcctcacccc aaaaatggca gcgctggcag
     9721 tccttgccat tgccgggatc ggggcagtaa cgggatgggc gatcagcccg agcgcgacgc
     9781 ccggaagcat tgacgtgccg caggtgctgg catcgacatt cagcgaccag gtgccgggca
     9841 gtgagggcgg cggcctgggt ggcggcctgc ccttcacttc ggccgtcggg gcattcacgg
     9901 acttcatggc ggggccggca atttttacct tgggcattct tggcatagtg gtcgcgggtg
     9961 ccgtgctcgt gttcgggggt gcgataaacc cagcgaacca tttgaggtga taggtaagat
    10021 tataccgagg tatgaaaacg agaattggac ctttacagaa ttactctatg aagcgccata
    10081 tttaaaaagc taccaagacg aagaggatga agaggatgag gaggcagatt gccttgaata
    10141 tattgacaat actgataaga taatatatct tttatataga agatatcgcc gtatgtaagg
    10201 atttcagggg gcaaggcata ggcagcgcgc ttatcaatat atctatagaa tgggcaaagc
    10261 ataaaaactt gcatggacta atgcttgaaa cccaggacaa taaccttata gcttgtaaat
    10321 tctatcataa ttgggtaatg actccaactt attgatagtg ttttatgttc agataatgcc
    10381 cgatgacttt gtcatgcagc tccaccgatt ttgagaacga cagcgacttc cgtcccagcc
    10441 gtgccaggtg ctgcctcaga ttcaggttat gccgctcaat tcgctgcgta tatcgcttgc
    10501 tgattacgtg cagctttccc ttcaggcggg attcatacag cggccagcca tccgtcatcc
    10561 atatcaccac gtcaaagggt gacagcaggc tcataagacg ccccagcgtc gccatagtgc
    10621 gttcaccgaa tacgtgcgca acaaccgtct tccggagact gtcatacgcg taaaacagcc
    10681 agcgctggcg cgatttagcc ccgacatagc cccactgttc gtccatttcc gcgcagacga
    10741 tgacgtcact gcccggctgt atgcgcgagg ttaccgactg cggcctgagt tttttaagtg
    10801 acgtaaaatc gtgttgaggc caacgcccat aatgcgggct gttgcccggc atccaacgcc
    10861 attcatggcc atatcaatga ttttctggtg cgtaccgggt tgagaagcgg tgtaagtgaa
    10921 ctgcagttgc catgttttac ggcagtgaga gcagagatag cgctgatgtc cggcggtgct
    10981 tttgccgtta cgcaccaccc cgtcagtagc tgaacaggag ggacagctga tagaaacaga
    11041 agccactgga gcacctcaaa aacaccatca tacactaaat cagtaagttg gcagcatcac
    11101 ccataattgt ggtttcaaaa tcggctccgt cgatactatg ttatacgcca actttgaaaa
    11161 caactttgaa aaagctgttt tctggtattt aaggttttag aatgcaagga acagtgaatt
    11221 ggagttcgtc ttgttataat tagcttcttg gggtatcttt aaatactgta gaaaagagga
    11281 aggaaataat aaatggctaa aatgagaata tcaccggaat tgaaaaaact gatcgaaaaa
    11341 taccgctgcg taaaagatac ggaaggaatg tctcctgcta aggtatataa gctggtggga
    11401 gaaaatgaaa acctatattt aaaaatgacg gacagccggt ataaagggac cacctatgat
    11461 gtggaacggg aaaaggacat gatgctatgg ctggaaggaa agctgcctgt tccaaaggtc
    11521 ctgcactttg aacggcatga tggctggagc aatctgctca tgagtgaggc cgatggcgtc
    11581 ctttgctcgg aagagtatga agatgaacaa agccctgaaa agattatcga gctgtatgcg
    11641 gagtgcatca ggctctttca ctccatcgac atatcggatt gtccctatac gaatagctta
    11701 gacagccgct tagccgaatt ggattactta ctgaataacg atctggccga tgtggattgc
    11761 gaaaactggg aagaagacac tccatttaaa gatccgcgcg agctgtatga ttttttaaag
    11821 acggaaaagc ccgaagagga acttgtcttt tcccacggcg acctgggaga cagcaacatc
    11881 tttgtgaaag atggcaaagt aagtggcttt attgatcttg ggagaagcgg cagggcggac
    11941 aagtggtatg acattgcctt ctgcgtccgg tcgatcaggg aggatatcgg ggaagaacag
    12001 tatgtcgagc tattttttga cttactgggg atcaagcctg attgggagaa aataaaatat
    12061 tatattttac tggatgaatt gttttagtac ctagatgtgg cgcaacgatg ccggcgacaa
    12121 gcaggagcgc accgacttct tccgcatcaa gtgttttggc tctcaggccg aggcccacgg
    12181 caagtatttg ggcaaggggt cgctggtatt cgtgcagggc aagattcgga ataccaagta
    12241 cgagaaggac ggccagacgg tctacgggac cgacttcatt gccgataagg tggattatct
    12301 ggacaccaag gcaccaggcg ggtcaaatca ggaataaggg cacattgccc cggcgtgagt
    12361 cggggcaatc ccgcaaggag ggtgaatgaa tcggacgttt gaccggaagg catacaggca
    12421 agaactgatc gacgcggggt tttccgccga ggatgccgaa accatcgcaa gccgcaccgt
    12481 catgcgtgcg ccccgcgaaa ccttccagtc cgtcggctcg atggtccagc aagctacggc
    12541 caagatcgag cgcgacagcg tgcaactggc tccccctgcc ctgcccgcgc catcggccgc
    12601 cgtggagcgt tcgcgtcgtc tcgaacagga ggcggcaggt ttggcgaagt cgatgaccat
    12661 cgacacgcga ggaactatga cgaccaagaa gcgaaaaacc gccggcgagg acctggcaaa
    12721 acaggtcagc gaggccaagc aggccgcgtt gctgaaacac acgaagcagc agatcaagga
    12781 aatgcagctt tccttgttcg atattgcgcc gtggccggac acgatgcgag cgatgccaaa
    12841 cgacacggcc cgctctgccc tgttcaccac gcgcaacaag aaaatcccgc gcgaggcgct
    12901 gcaaaacaag gtcattttcc acgtcaacaa ggacgtgaag atcacctaca ccggcgtcga
    12961 gctgcgggcc gacgatgacg aactggtgtg gcagcaggtg ttggagtacg cgaagcgcac
    13021 ccctatcggc gagccgatca ccttcacgtt ctacgagctt tgccaggacc tgggctggtc
    13081 gatcaatggc cggtattaca cgaaggccga ggaatgcctg tcgcgcctac aggcgacggc
    13141 gatgggcttc acgtccgacc gcgttgggca cctggaatcg gtgtcgctgc tgcaccgctt
    13201 ccgcgtcctg gaccgtggca agaaaacgtc ccgttgccag gtcctgatcg acgaggaaat
    13261 cgtcgtgctg tttgctggcg accactacac gaaattcata tgggagaagt accgcaagct
    13321 gtcgccgacg gcccgacgga tgttcgacta tttcagctcg caccgggagc cgtacccgct
    13381 caagctggaa accttccgcc tcatgtgcgg atcggattcc acccgcgtga agaagtggcg
    13441 cgagcaggtc ggcgaagcct gcgaagagtt gcgaggcagc ggcctggtgg aacacgcctg
    13501 ggtcaatgat gacctggtgc attgcaaacg ctagggcctt gtggggtcag ttccggctgg
    13561 gggttcagca gccagcgctt tactggcatt tcaggaacaa gcgggcactg ctcgacgcac
    13621 ttgcttcgct cagtatcgct cgggacgcac ggcgcgctct acgaactgcc gataaacaga
    13681 ggattaaaat tgacaattgt gattaaggct cagattcgac ggcttggagc ggccgacgtg
    13741 caggatttcc gcgagatccg attgtcggcc ctgaagaaag ctccagagat gttcgggtcc
    13801 gtttacgagc acgaggagaa aaagcccatg gaggcgttcg ctgaacggtt gcgagatgcc
    13861 gtggcattcg gcgcctacat cgacggcgag atcattgggc tgtcggtctt caaacaggag
    13921 gacggcccca aggacgctca caaggcgcat ctgtccggcg ttttcgtgga gcccgaacag
    13981 cgaggccgag gggtcgccgg tatgctgctg cgggcgttgc cggcgggttt attgctcgtg
    14041 atgatcgtcc gacagattcc aacgggaatc tggtggatgc gcatcttcat cctcggcgca
    14101 cttaatattt cgctattctg gagcttgttg tttatttcgg tctaccgcct gccgggcggg
    14161 gtcgcggcga cggtaggcgc tgtgcagccg ctgatggtcg tgttcatctc tgccgctctg
    14221 ctaggtagcc cgatacgatt gatggcggtc ctgggggcta tttgcggaac tgcgggcgtg
    14281 gcgctgttgg tgttgacacc aaacgcagcg ctagatcctg tcggcgtcgc agcgggcctg
    14341 gcgggggcgg tttccatggc gttcggaacc gtgctgaccc gcaagtggca acctcccgtg
    14401 cctctgctca cctttaccgc ctggcaactg gcggccggag gacttctgct cgttccagta
    14461 gctttagtgt ttgatccgcc aatcccgatg cctacaggaa ccaatgttct cggcctggcg
    14521 tggctcggcc tgatcggagc gggtttaacc tacttccttt ggttccgggg gatctcgcga
    14581 ctcgaaccta cagttgtttc cttactgggc tttctcagcc ccagatctgg ggtcgatcag
    14641 ccggggatgc atcaggccga cagtcggaac ttcgggtccc cgacctgtac cattcggtga
    14701 gcaatggata ggggagttga tatcgtcaac gttcacttct aaagaaatag cgccactcag
    14761 cttcctcagc ggctttatcc agcgatttcc tattatgtcg gcatagttct caagatcgac
    14821 agcctgtcac ggttaagcga gaaatgaata agaaggctga taattcggat ctctgcgagg
    14881 gagatgatat ttgatcacag gcagcaacgc tctgtcatcg ttacaatcaa catgctaccc
    14941 tccgcgagat catccgtgtt tcaaacccgg cagcttagtt gccgttcttc cgaatagcat
    15001 cggtaacatg agcaaagtct gccgccttac aacggctctc ccgctgacgc cgtcccggac
    15061 tgatgggctg cctgtatcga gtggtgattt tgtgccgagc tgccggtcgg ggagctgttg
    15121 gctggctggt ggcaggatat attgtggtgt aaacaaattg acgcttagac aacttaataa
    15181 cacattgcgg acgtttttaa tgtactgggg tggtttttct tttcaccagt gagacgggca
    15241 acagctgatt gcccttcacc gcctggccct gagagagttg cagcaagcgg tccacgctgg
    15301 tttgccccag caggcgaaaa tcctgtttga tggtggttcc gaaatcggca aaatccctta
    15361 taaatcaaaa gaatagcccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc
    15421 actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg
    15481 cccactacgt gaaccatcac ccaaatcaag ttttttgggg tcgaggtgcc gtaaagcact
    15541 aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt
    15601 ggcgagaaag gaagggaaga aagcgaaagg agcgggcgcc attcaggctg cgcaactgtt
    15661 gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg
    15721 ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga
    15781 cggccagtga attaattccc gatctagtaa catagatgac accgcgcgcg ataatttatc
    15841 ctagtttgcg cgctatattt tgttttctat cgcgtattaa atgtataatt gcgggactct
    15901 aatcataaaa acccatctca taaataacgt catgcattac atgttaatta ttacatgctt
    15961 aacgtaattc aacagaaatt atatgataat catcgcaaga ccggcaacag gattcaatct
    16021 taagaaactt tattgccaaa tgtttgaacg atcggggaaa ttcgagctcg gtaccccctc
    16081 ctgggcacgg tcgtacgcga tccgccgcgg ttccacctca ccattgcggg tatgccagtt
    16141 gagttcgatg agttcccgta ccagctccaa ctcgggcgat gccacgcgca ctccgttcag
    16201 atctcggtga cgggcaggac cggacggggc ggtaccggca ggctgaagtc cagctgccag
    16261 aaacccacgt catgccagtt cccgtgcttg aagccggccg cccgcagcat gccgcggggg
    16321 gcatatccga gcgcctcgtg catgcgcacg ctcgggtcgt tgggcagccc gatgacagcg
    16381 accacgctct tgaagccctg tgcctccagg gacttcagca ggtgggtgta gagcgtggag
    16441 cccagtcccg tccgctggtg gcggggggag acgtacacgg tcgactcggc cgtccagtcg
    16501 taggcgttgc gtgccttcca ggggcccgcg taggcgatgc cggcgacctc gccgtccacc
    16561 tcggcgacga gccagggata gcgctcccgc agacggacga ggtcgtccgt ccactcctgc
    16621 ggttcctgcg gctcggtacg gaagttgacc gtgcttgtct cgatgtagtg gttgacgatg
    16681 gtgcagaccg ccggcatgtc cgcctcggtg gcacggcgga tgtcggccgg gcgtcgttct
    16741 gggctcatgg atcctctaga gtcccccgtg ttctctccaa atgaaatgaa cttccttata
    16801 tagaggaagg gtcttgcgaa ggatagtggg attgtgcgtc atcccttacg tcagtggaga
    16861 tatcacatca atccacttgc tttgaagacg tggttggaac gtcttctttt tccacgatgc
    16921 tcctcgtggg tgggggtcca tctttgggac cactgtcggc agaggcatct tcaacgatgg
    16981 cctttccttt atcgcaatga tggcatttgt aggagccacc ttccttttcc actatcttca
    17041 caataaagtg acagatagct gggcaatgga atccgaggag gtttccggat attacccttt
    17101 gttgaaaagt ctcaattgcc ctttggtctt ctgagactgt atctttgata tttttggagt
    17161 agacaagtgt gtcgtgctcc accatgttga cgaagatttt cttcttgtca ttgagtcgta
    17221 agagactctg tatgaactgt tcgccagtct ttacggcgag ttctgttagg tcctctattt
    17281 gaatctttga ctccatggcc tttgattcag tgggaactac ctttttagag actccaatct
    17341 ctattacttg ccttggtttg tgaagcaagc cttgaatcgt ccatactgga atagtacttc
    17401 tgatcttgag aaatatatct ttctctgtgt tcttgatgca gttagtcctg aatcttttga
    17461 ctgcatcttt aaccttcttg ggaaggtatt tgatttcctg gagattattg ctcgggtaga
    17521 tcgtcttgat gagacctgct gcgtaagcct ctctaaccat ctgtgggtta gcattctttc
    17581 tgaaattgaa aaggctaatc tggggacctg caggggcaat accgccaggg ttttcccagt
    17641 cacgacgttg taaaacgacg gccagtgcca agctttcgac gagtcagtaa taaacggcgt
    17701 caaagtggtt gcagccggca cacacgagtc gtgtttatca actcaaagca caaatacttt
    17761 tcctcaacct aaaaataagg caattagcca aaaacaactt tgcgtgtaaa caacgctcaa
    17821 tacacgtgtc attttattat tagctattgc ttcaccgcct tagctttctc gtgacctagt
    17881 cgtcctcgtc ttttcttctt cttcttctat aaaacaatac ccaaagagct cttcttcttc
    17941 acaattcaga tttcaatttc tcaaaatctt aaaaactttc tctcaattct ctctaccgtg
    18001 atcaaggtaa atttctgtgt tccttattct ctcaaaatct tcgattttgt tttcgttcga
    18061 tcccaatttc gtatatgttc tttggtttag attctgttaa tcttagatcg aagacgattt
    18121 tctgggtttg atcgttagat atcatcttaa ttctcgatta gggtttcata gatatcatcc
    18181 gatttgttca aataatttga gttttgtcga ataattactc ttcgatttgt gatttctatc
    18241 tagatctggt gttagtttct agtttgtgcg atcgaatttg tagattaatc tgagtttttc
    18301 tgattaacag ggtaccgggc cccccctcga ggcgcg
//
