LOCUS       sequence-202788-        4234 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses PHLDB1, puromycin resistant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4234)
  AUTHORS   Aries IM, Bodaar K, Karim SA, Chonghaile TN, Hinze L, Burns MA, 
            Pfirrmann M, Degar J, Landrigan JT, Balbach S, Peirs S, Menten B, 
            Isenhart R, Stevenson KE, Neuberg DS, Devidas M, Loh ML, Hunger SP, 
            Teachey DT, Rabin KR, Winter SS, Dunsmore KP, Wood BL, Silverman LB,
            Sallan SE, Van Vlierberghe P, Orkin SH, Knoechel B, Letai AG, 
            Gutierrez A
  TITLE     PRC2 loss induces chemoresistance by repressing apoptosis in T cell 
            acute lymphoblastic leukemia.
  JOURNAL   J Exp Med. 2018 Nov 7. pii: jem.20180570. doi: 10.1084/jem.20180570.
  PUBMED    30404791
REFERENCE   2  (bases 1 to 4234)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp Med. 
            2018 Nov 7. pii: jem.20180570. doi: 10.1084/jem.20180570."
COMMENT     Sequence Label: pLenti CMV Puro DEST (w118-1) PHLDB1
FEATURES             Location/Qualifiers
     source          1..4234
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(4139..4163)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 accatggatg ccttgaatcg aaatcagata gggccggggt gccagactca gaccatggtt
       61 cagaaggggc ccctagattt aattgaaacc ggtaaagggc tcaaggtgca aacggataag
      121 cctcatttag tttcacttgg atcaggccgc ctctcaaccg ccatcacctt actacccctc
      181 gaagaaggac ggacagttat cggatcagcc gcaagagaca tatctctcca gggcccgggt
      241 ctggccccag agcattgcta tatcgagaac ctacgaggga ctctaactct gtacccatgt
      301 ggtaacgcat gcaccatcga cggacttccc gtccgccaac cgacacgtct gacacagggc
      361 tgcatgctgt gtctgggcca gtccactttc ttaaggttca accatccagc agaagcgaag
      421 tggatgaaaa gtatgatccc ggctggagga cgcgctccgg gaccgccata ctcccctgtc
      481 ccagccgaaa gtgagtcgct ggtcaatggt aaccacactc cacagaccgc tactcgcggt
      541 ccttccgctt gcgcctccca ttcaagtctt gtgtcctcga ttgaaaagga tctccaagaa
      601 attatggatt cgctggtcct cgaggaacca ggcgcggcgg gcaaaaagcc ggcagcaaca
      661 agtcccctct cgccaatggc gaacggtggg cgctacttgc tgtccccacc aacttcgcct
      721 ggagcaatgt ccgtaggttc cagttatgag aatacctcgc ccgctttctc acccctatct
      781 tctcccgctt catcaggctc ctgtgctagc cacagtccta gtgggcaaga gcccggacct
      841 tcagtcccac ctctggtacc tgcgaggtcc agctcttatc acctggcact gcaaccgccg
      901 cagtcccgcc catctggtgc cagaagcgaa agcccacgac ttagtagaaa gggtgggcac
      961 gaacgtcctc ccagcccagg actccgggga ttattgacag attcccctgc ggccacagta
     1021 ctcgctgagg cgcgccgcgc cactgagagt ccacgtctgg gaggacagtt gccagtcgtg
     1081 gccataagct tgtctgagta ccccgcatca ggtgctctct cccaacctac cagtatacca
     1141 ggctcaccga agttccaacc gccggtccct gcccctcgga acaagatcgg cacactccag
     1201 gacaggccgc cttctccctt tagggaacca ccaggatccg aacgcgtttt gaccaccagc
     1261 ccctctagac agttagttgg aagaactttc agtgacggtc ttgccaccag aacccttcag
     1321 ccacccgaaa gtcctaggtt gggccggagg ggcctggata gtatgcgtga acttccacca
     1381 ctaagcccga gcttgtcacg gagggcgcta tcacccctgc cgaccaggac tacaccggat
     1441 cctaagttga acagggaagt tgcagaatca cccaggccac gtcgctgggc tgcacacggc
     1501 gctagtcccg aggacttctc tctgactctt ggagcgcgtg gccgacgcac caggagccct
     1561 tcccctacac ttggcgaaag ccttgctcca cacaagggga gttttagtgg aagactgagc
     1621 cctgcctact ccttggggag tctcaccggc gcttcaccgt gccaatcgcc atgtgttcaa
     1681 cgaaaattat catcaggaga cctacgagtg ccagtgacga gagaaagaaa gaattccatt
     1741 actgaaatca gcgacaatga agacgacctg ctcgaatacc accgacgaca acggcaagaa
     1801 agactccgag aacaagagat ggagagactt gagcgccagc ggctggaaac cattctcaat
     1861 ttatgtgctg agtattctcg cgcagatggc ggccctgaag cgggtgaact gcctagcata
     1921 ggcgaagcca cagccgctct tgctcttgca ggccgccgac ctagtcgagg attggccggc
     1981 gcgagtggac gctcgtcaga agagccggga gtggcgactc agagactgtg ggaaagcatg
     2041 gagcggtcgg acgaggagaa cctgaaagag gaatgtagtt caaccgagag tacccagcaa
     2101 gagcacgagg atgctccctc tacgaaattg cagggcgaag tactggctct tgaggaggag
     2161 agagctcagg tattaggtca tgttgagcaa cttaaggtga gggtgaaaga gctggaacaa
     2221 caacttcagg agagtgcaag ggaagcagaa atggagaggg cattacttca gggcgagcgc
     2281 gaggctgaga gagctctctt gcagaaggag cagaaggctg tggaccagct acaggagaaa
     2341 cttgttgcct tagagacggg aattcagaag gaaagggata aagaggcgga agccctcgag
     2401 acagagacaa aacttttcga ggatctcgaa tttcaacagc tcgaaagaga gagtcgagtg
     2461 gaagaggaac gtgagcttgc tggacagggg cttctgagaa gtaaggcaga actattgcgt
     2521 tccattgcta aaaggaaaga gcgactggct atcctggata gccaggcagg acagatcaga
     2581 gctcaggcag ttcaagaaag cgaacgctta gcacgcgaca agaacgcgtc actgcaactg
     2641 ctgcagaagg aaaaagagaa gctcacggtt ttggaacgtc gatatcactc tctgaccgga
     2701 ggcagaccat tccccaaaac tacgtccaca ctcaaggaga tggaaaagtt attgctacct
     2761 gctgtggacc tcgaacagtg gtatcaagaa cttatggccg gactaggcac gggtcccgcc
     2821 gccgccagtc ctcactcttc gcctccacca ttacccgcca aagcgtcccg gcagcttcag
     2881 gtttacagga gcaagatgga tggcgaggca acgtctcctt tgccacgcac tcgctcaggg
     2941 ccgttaccca gctcttccgg ttcgagtagt tcaagctctc agctttcggt ggccacgcta
     3001 gggcgatccc ctagtccgaa gtctgccctg ttgacccaga atggcacagg ttcattacca
     3061 cgcaatctgg cagccaccct gcaggatatt gagaccaagc gccagctggc gttgcagcag
     3121 aagggccagc aagtgattga ggaacagaga aggagactag cagaattaaa acagaaggcc
     3181 gccgctgaag cccagtgcca atgggacgcc ctccacggag ctgcaccttt tcctgctggg
     3241 ccaagcggat ttccacccct catgcaccac tctatactgc atcatctgcc cgcaggacgt
     3301 gagcgcggag aagagggcga gcacgcctat gatactttga gtcttgagtc aagcgatagc
     3361 atggagacat ccatctcgac gggtgggaac agcgcctgct cacccgacaa tatgtcctcc
     3421 gccagcggct tagatatggg caaaattgag gagatggaga agatgcttaa agaggctcac
     3481 gcagagaaga accggctgat ggagtcaagg gagcgggaaa tggagctacg gagacaagcc
     3541 cttgaggaag aacgtagacg gcgcgagcaa gtggagagga ggctgcagag tgaatctgct
     3601 aggaggcaac agctcgttga aaaagaggtt aaaatgagag aaaaacagtt ttcacaagct
     3661 aggcccctga ctcggtacct tcctattagg aaagaagact ttgacctgaa gacgcacatc
     3721 gaaagcagcg ggcacggtgt cgacacctgc ctgcacgtgg tgctcagcag caaagtgtgc
     3781 agagggtatt tggtgaagat gggtggcaag atcaaatcat ggaagaaacg ctggtttgtc
     3841 ttcgatcgct tgaagcgtac tctctcctat tacgttgaca agcacgagac caagttgaaa
     3901 ggggtaatct attttcaggc catcgaggag gtgtactatg accatctgcg tagtgccgca
     3961 aagaaacggt tcttccggtt caccatggtt acagaatcgc cgaaccctgc cctgaccttc
     4021 tgcgtgaaaa ctcacgatcg tctgtattac atggtggccc cgtccgcgga ggcgatgcgc
     4081 atctggatgg atgtgatcgt tacaggtgcg gaaggataca cccagttcat gaattgaaac
     4141 ccagctttct tgtacaaagt tggcattata agaaagcatt gcttatcaat ttgttgcaac
     4201 gaacaggtca ctatcagtca aaataaaatc atta
//
