LOCUS       sequence-202797-        6423 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 485, Emission: 507.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6423)
  TITLE     Davidson Green fluorescent proteins
REFERENCE   2  (bases 1 to 6423)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: sfGFP-Zyxin-6
FEATURES             Location/Qualifiers
     source          1..6423
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      574..583
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      2304..2313
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2310..2312,2313..2315,2316..3029)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIK
                     ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(2355..2376)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     2963..2984
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    3151..3272
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3188..3207)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3242..3261
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3279..3734)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3416..3437)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3628..3647
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3761..3865
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3867..4224
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3867..4058
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3890..3910)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4075..4210
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4259..5053
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4259..5053
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4313..4332)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4923..4942
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5241..5260)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5285..5332
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5661..6249
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6150..6169
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      541 ccggactcag atctcgagct caagcttcga attccatggc ggccccccgc ccgtctcccg
      601 cgatccccgt ttcggtctcg gctccggctt tttacgcccc gcagaagaag ttcggccctg
      661 tggtggcccc aaagcccaaa gtgaatccct tccggcccag ggacagcgag cctcccccgg
      721 cacccggggc ccagcgcgca cagatgggcc gggtgggcga tattcccccg ccgcccccgg
      781 aagactttcc cctgcctcca cctccccttg ctggggatgg cgacgatgca gagggtgctc
      841 tgggaggtgc cttcccgccg ccccctcccc cgatcgagga atcatttccc cctgcgcctc
      901 tggaggagga gatcttccct tccccgccgc ctcctccgga ggaggaggga gggcctgagg
      961 cccccatacc gcccccacca cagcccaggg agaaggtgag cagtattgat ttggagatcg
     1021 actctctgtc ctcactgctg gatgacatga ccaagaatga tcctttcaaa gcccgggtgt
     1081 catctggata tgtgccccca ccagtggcca ctccattcag ttccaagtcc agtaccaagc
     1141 ctgcagccgg gggcacagca cccctgcctc cttggaagtc cccttccagc tcccagcctc
     1201 tgccccaggt tccggctccg gctcagagcc agacacagtt ccatgttcag ccccagcccc
     1261 agcccaagcc tcaggtccaa ctccatgtcc agtcccagac ccagcctgtg tctttggcta
     1321 acacccagcc ccgagggccc ccagcctcat ctccggctcc agcccctaag ttttctccag
     1381 tgactcctaa gtttactcct gtggcttcca agttcagtcc tggagcccca ggtggatctg
     1441 ggtcacaacc aaatcaaaaa ttggggcacc ccgaagctct ttctgctggc acaggctccc
     1501 ctcaacctcc cagcttcacc tatgcccagc agagggagaa gccccgagtg caggagaagc
     1561 agcaccccgt gcccccaccg gctcagaacc aaaaccaggt gcgctcccct ggggccccag
     1621 ggcccctgac tctgaaggag gtggaggagc tggagcagct gacccagcag ctaatgcagg
     1681 acatggagca tcctcagagg cagaatgtgg ctgtcaacga actctgcggc cgatgccatc
     1741 aacccctggc ccgggcgcag ccagccgtcc gcgctctagg gcagctgttc cacatcgcct
     1801 gcttcacctg ccaccagtgt gcgcagcagc tccagggcca gcagttctac agtctggagg
     1861 gggcgccgta ctgcgagggc tgttacactg acaccctgga gaagtgtaac acctgcgggg
     1921 agcccatcac tgaccgcatg ctgagggcca cgggcaaggc ctatcacccg cactgcttca
     1981 cctgtgtggt ctgcgcccgc cccctggagg gcacctcctt catcgtggac caggccaacc
     2041 ggccccactg tgtccccgac taccacaagc agtacgcccc gaggtgctcc gtctgctctg
     2101 agcccatcat gcctgagcct ggccgagatg agactgtgcg agtggtcgcc ctggacaaga
     2161 acttccacat gaagtgttac aagtgtgagg actgcgggaa gcccctgtcg attgaggcag
     2221 atgacaatgg ctgcttcccc ctggacggtc acgtgctctg tcggaagtgc cacactgcta
     2281 gagcccagac ggatccaccg gtcgccacca tggtgagcaa gggcgaggag ctgttcaccg
     2341 gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgc
     2401 gcggcgaggg cgagggcgat gccaccaacg gcaagctgac cctgaagttc atctgcacca
     2461 ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt
     2521 gcttcagccg ctaccccgac cacatgaagc gccacgactt cttcaagtcc gccatgcccg
     2581 aaggctacgt ccaggagcgc accatcagct tcaaggacga cggcacctac aagacccgcg
     2641 ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact
     2701 tcaaggagga cggcaacatc ctggggcaca agctggagta caacttcaac agccacaacg
     2761 tctatatcac cgccgacaag cagaagaacg gcatcaaggc caacttcaag atccgccaca
     2821 acgtggagga cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg
     2881 acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgtg ctgagcaaag
     2941 accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca
     3001 ctcacggcat ggacgagctg tacaagtaag cggccgcgac tctagatcat aatcagccat
     3061 accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg
     3121 aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac
     3181 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     3241 tgtggtttgt ccaaactcat caatgtatct taaggcgtaa attgtaagcg ttaatatttt
     3301 gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat
     3361 cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt
     3421 ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt
     3481 ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag
     3541 gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg
     3601 aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc
     3661 gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc
     3721 gctacagggc gcgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     3781 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     3841 tcaataatat tgaaaaagga agagtcctga ggcggaaaga accagctgtg gaatgtgtgt
     3901 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     3961 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     4021 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
     4081 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     4141 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
     4201 tttggaggcc taggcttttg caaagatcga tcaagagaca ggatgaggat cgtttcgcat
     4261 gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg
     4321 ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc
     4381 gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca
     4441 agacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct
     4501 cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga
     4561 tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg
     4621 gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat
     4681 cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga
     4741 gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca tgcccgacgg
     4801 cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg
     4861 ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat
     4921 agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct
     4981 cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga
     5041 cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac gcccaacctg
     5101 ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt
     5161 ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc
     5221 caccctaggg ggaggctaac tgaaacacgg aaggagacaa taccggaagg aacccgcgct
     5281 atgacggcaa taaaaagaca gaataaaacg cacggtgttg ggtcgtttgt tcataaacgc
     5341 ggggttcggt cccagggctg gcactctgtc gataccccac cgagacccca ttggggccaa
     5401 tacgcccgcg tttcttcctt ttccccaccc caccccccaa gttcgggtga aggcccaggg
     5461 ctcgcagcca acgtcggggc ggcaggccct gccatagcct caggttactc atatatactt
     5521 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     5581 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     5641 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     5701 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     5761 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     5821 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     5881 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     5941 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     6001 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     6061 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     6121 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     6181 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     6241 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     6301 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccatg
     6361 cattagttat taatagtaat caattacggg gtcattagtt catagcccat atatggagtt
     6421 ccg
//
