LOCUS       sequence-20280-e         746 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 746)
  AUTHORS   Nicke B, Bastien J, Khanna SJ, Warne PH, Cowling V, Cook SJ, Peters 
            G, Delpuech O, Schulze A, Berns K, Mullenders J, Beijersbergen RL, 
            Bernards R, Ganesan TS, Downward J, Hancock DC
  TITLE     Involvement of MINK, a Ste20 family kinase, in Ras oncogene-induced 
            growth arrest in human ovarian surface epithelial cells.
  JOURNAL   Mol Cell. 2005 Dec 9. 20(5):673-85.
  PUBMED    16337592
REFERENCE   2  (bases 1 to 746)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2005 Dec 9. 20(5):673-85."
COMMENT     Sequence Label: pMig (Martins Lab)
FEATURES             Location/Qualifiers
     source          1..746
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     5..21
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     promoter        complement(37..251)
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     complement(97..116)
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     primer_bind     complement(297..320)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     694..713
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
ORIGIN
        1 tctctcgagg tcgacggtat ccataagctt agatctgtgg tctcatacag aacttataag
       61 attcccaaat ccaaagacat ttcacgttta tggtgatttc ccagaacaca tagcgacatg
      121 caaatattgc agggcgccac tcccctgtcc ctcacagcca tcttcctgcc agggcgcacg
      181 cgcgctgggt gttcccgcct agtgacactg ggcccgcgat tccttggagc gggttgatga
      241 cgtcagcgtt cgaattctac cgggtagggg aggcgctttt cccaaggcag tctggagcat
      301 gcgctttagc agccccgctg ggcacttggc gctacacaag tggcctctgg cctcgcacac
      361 attccacatc caccggtagg cgccaaccgg ctccgttctt tggtggcccc ttcgcgccac
      421 cttctactcc tcccctagtc aggaagttcc cccccgcccc gcagctcgcg tcgtgcagga
      481 cgtgacaaat ggaagtagca cgtctcacta gtctcgtgca gatggacagc accgctgagc
      541 aatggaagcg ggtaggcctt tggggcagcg gccaatagca gctttgctcc ttcgctttct
      601 gggctcagag gctgggaagg ggtgggtccg ggggcgggct caggggcggg ctcaggggcg
      661 gggcgggcgc ccgaaggtcc tccggaggcc cggcattctg cacgcttcaa aagcgcacgt
      721 ctgccgcgct gttctcctct tcctca
//
