LOCUS       sequence-202813-        4046 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Negative regulator of cell cycle progression by G1/S transition 
            inhibition.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4046)
  AUTHORS   Razy-Krajka F, Gravez B, Kaplan N, Racioppi C, Wang W, Christiaen L
  TITLE     An FGF-driven feed-forward circuit patterns the cardiopharyngeal 
            mesoderm in space and time.
  JOURNAL   Elife. 2018 Feb 6;7. pii: 29656. doi: 10.7554/eLife.29656.
  PUBMED    29431097
REFERENCE   2  (bases 1 to 4046)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2018-02-6"; volume: "7. pii: 29656. doi"; pages: " 
            10.7554/eLife.29656"
COMMENT     Sequence Label: FoxFTVC+bpFOG_Cdkn1b.b
FEATURES             Location/Qualifiers
     source          1..4046
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    1772..1906
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1809..1828)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1863..1882
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1942..1961)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1943..1961)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2048..2065)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2219..2807)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2299..2318)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2978..3769,3770..3838))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3601..3620
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3839..3943)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4011..4029
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ggcgcgccgc gttgtatctt tcgttaagtg ctggcgtcga gcagggctaa cctcatttcg
       61 atttgacgat tcgggtaaaa ccgagcgatg gacaggacct ggaaatggga aaggcttact
      121 agaatcaggg tcgaacgaga taagaagatc aggattcgca aattagcact tggctttttt
      181 acgtagaaac ataggatgtc cgctgagccc cttatatata cgtagtacga atcaccggcc
      241 tggcgttgta taagcgagac cagcgtggac gaaaaggtaa ttgtttccga atggatcgcg
      301 ttctctagag gatccggcaa agcttcgtgt attgtaccgg cccattgtca atcatgcaaa
      361 cttgatatta tattgacaag agaagaaggc agtttaaatt aaaactctaa agtagagaga
      421 cattaatctc agctgacaag gcaggtggtc acagtaagtt catttaaata gttggccaac
      481 aatagccttt ccaagaaagt atttttgttc caggtctata caaaaataac acacaagcgg
      541 ccgcatggtt cccccacctt cgtacaatgc ccacgaaaca tcagcattcg ctgtttttga
      601 gaagccagtg ctagtgaagc ccccaagtcc tgtttgtcgc caactatttg gtctttcaga
      661 tcaattagag acgagcagac gacttaaaga ggttttggat tctgaagcta aaagatttca
      721 gttcgcctgg aacttcgacg tccgtaaagt tccagctgcg ggaaatcagt cgccggatcc
      781 cggggcagct gcaccgggca aaccgaagcg ttttgagtgg aagttacaac aaagaagtga
      841 agtgccccac ttctacactc gaccctttgc aacccacagg ttggcaatga cgtcacctcg
      901 tctcgaccgt tccccgttga aaaagaaccg cacccagaaa agccaccacg atagacacct
      961 aacaaacact tcctctccga cgccccttag tcaacgagat gaaaacgtct cccgatcgat
     1021 cataaatcca tctaaacgac tttgcacccc cctttttacg agcccaccta agctaaccac
     1081 cgaagcgaag ccagacttga ccagagttcc tgcaatgaga attcccccga ttacgccaag
     1141 cgccgactcc aacaagccca gcatggttag tgacgacatg gaaaaggcaa ccgcatcgga
     1201 gccagtcggg tccatggtcg cttccaactc attgaagcgg cctgcctctc gaaagttgtt
     1261 caacctgagc actccaagtc caaagcgatc gcgaactagc agccgacaac cgaaaataac
     1321 agacgtgatg ccacaacgaa aactccgttc aacgtcgaac aacaaaagcg aaggcaataa
     1381 aactgtgaac cccataaact ctgcctcttt gcaagcagcc aaagcaagta attgactcca
     1441 cctagaggcc agaattccag ctgagcgccg gtcgctacca ttaccagttg gtctggtgtc
     1501 aaaaataata ataaccgggc aggccatgtc tgcccgtatt tcgcgtaagg aaatccatta
     1561 tgtactattt aaaaaacaca aacttttgga tgttcggttt attctttttc ttttactttt
     1621 ttatcatggg agcctacttc ccgtttttcc cgatttggct acatgacatc aaccatatca
     1681 gcaaaagtga tacgggtatt atttttgccg ctatttctct gttctcgcta ttattccaac
     1741 cgctgtttgg tctgctttct gacaaactcg gaacttgttt attgcagctt ataatggtta
     1801 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     1861 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatcgaca aagtcaaagc
     1921 ggccatcaga tctgccggtc tccctatagt gagtcgtatt aatttcgata agccaggtta
     1981 acctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
     2041 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     2101 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     2161 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     2221 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     2281 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     2341 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     2401 tggcgctttc tcaatgctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     2461 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     2521 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     2581 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     2641 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct
     2701 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     2761 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     2821 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     2881 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     2941 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     3001 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     3061 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     3121 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     3181 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     3241 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     3301 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     3361 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     3421 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     3481 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     3541 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     3601 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     3661 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     3721 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     3781 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     3841 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     3901 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     3961 tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta
     4021 tcacgaggcc cttacgtatt aattaa
//
