LOCUS       sequence-202819-        9443 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses human codon optimized FnCpf1(RVR mutant) in mammalian 
            cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9443)
  AUTHORS   Toth E, Czene BC, Kulcsar PI, Krausz SL, Talas A, Nyeste A, Varga E,
            Huszar K, Weinhardt N, Ligeti Z, Borsy AE, Fodor E, Welker E
  TITLE     Mb- and FnCpf1 nucleases are active in mammalian cells: activities 
            and PAM preferences of four wild-type Cpf1 nucleases and of their 
            altered PAM specificity variants.
  JOURNAL   Nucleic Acids Res. 2018 Sep 20. pii: 5103950. doi: 
            10.1093/nar/gky815.
  PUBMED    30239882
REFERENCE   2  (bases 1 to 9443)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2018 Sep 20. pii: 5103950. doi: 10.1093/nar/gky815."
COMMENT     Sequence Label: pTE3314
FEATURES             Location/Qualifiers
     source          1..9443
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             4829..4876
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             4883..4963
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     primer_bind     complement(5037..5054)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5043..5267
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5313..5741
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5400..5419)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5610..5631
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5750..5770)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5755..6084
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5935..6070
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5997..6016
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6151..6945
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6205..6224)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6815..6834
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7119..7240
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7156..7175)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7210..7229
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7289..7305)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7289..7305)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7302..7324)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7313..7329
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7337..7343,7344..7361,7362..7367))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7382..7403
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7520..7537)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7691..8276)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7771..7790)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8447..9238,9239..9307))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9070..9089
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9308..9412)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttggta ccgccaccat gagcatctac caggagttcg tcaacaagta
      961 ttcactgagt aagacactgc ggttcgagct gatcccacag ggcaagacac tggagaacat
     1021 caaggcccga ggcctgattc tggacgatga gaagcgggca aaagactata agaaagccaa
     1081 gcagatcatt gataaatacc accagttctt tatcgaggaa attctgagct ccgtgtgcat
     1141 cagtgaggat ctgctgcaga attactcaga cgtgtacttc aagctgaaga agagcgacga
     1201 tgacaacctg cagaaggact tcaagtccgc caaggacacc atcaagaaac agattagcga
     1261 gtacatcaag gactccgaaa agtttaaaaa tctgttcaac cagaatctga tcgatgctaa
     1321 gaaaggccag gagtccgacc tgatcctgtg gctgaaacag tctaaggaca atgggattga
     1381 actgttcaag gctaactccg atatcactga tattgacgag gcactggaaa tcatcaagag
     1441 cttcaaggga tggaccacat actttaaagg cttccacgag aaccgcaaga acgtgtactc
     1501 cagcaacgac attcctacct ccatcatcta ccgaatcgtc gatgacaatc tgccaaagtt
     1561 cctggagaac aaggccaaat atgaatctct gaaggacaaa gctcccgagg caattaatta
     1621 cgaacagatc aagaaagatc tggctgagga actgacattc gatatcgact ataagactag
     1681 cgaggtgaac cagagggtct tttccctgga cgaggtgttt gaaatcgcca atttcaacaa
     1741 ttacctgaac cagtccggca ttactaaatt caataccatc attggcggga agtttgtgaa
     1801 cggggagaat accaagcgca agggaattaa cgaatacatc aatctgtata gccagcagat
     1861 caacgacaaa actctgaaga aatacaagat gtctgtgctg ttcaaacaga tcctgagtga
     1921 taccgagtcc aagtcttttg tcattgataa actggaagat gactcagacg tggtcactac
     1981 catgcagagc ttttatgagc agatcgccgc tttcaagaca gtggaggaaa aatctattaa
     2041 ggaaactctg agtctgctgt tcgatgacct gaaagcccag aagctggacc tgagtaagat
     2101 ctacttcaaa aacgataaga gtctgacaga cctgtcacag caggtgtttg atgactattc
     2161 cgtgattggg accgccgtcc tggagtacat tacacagcag atcgctccaa agaacctgga
     2221 taatccctct aagaaagagc aggaactgat cgctaagaaa accgagaagg caaaatatct
     2281 gagtctggaa acaattaagc tggcactgga ggagttcaac aagcacaggg atattgacaa
     2341 acagtgccgc tttgaggaaa tcctggccaa cttcgcagcc atccccatga tttttgatga
     2401 gatcgcccag aacaaagaca atctggctca gatcagtatt aagtaccaga accagggcaa
     2461 gaaagacctg ctgcaggctt cagcagaaga tgacgtgaaa gccatcaagg atctgctgga
     2521 ccagaccaac aatctgctgc acaagctgaa aatcttccat attagtcagt cagaggataa
     2581 ggctaatatc ctggataaag acgaacactt ctacctggtg ttcgaggaat gttacttcga
     2641 gctggcaaac attgtccccc tgtataacaa gattaggaac tacatcacac agaagcctta
     2701 ctctgacgag aagtttaaac tgaacttcga aaatagtacg ctagccaggg ggtgggataa
     2761 gaacgtggag cctgacagga cagctatcct gttcatcaag gatgacaagt actatctggg
     2821 agtgatgaat aagaaaaaca ataagatctt cgatgacaaa gccattaagg agaacaaagg
     2881 ggaaggatac aagaaaatcg tgtataagct gctgcccggc gcaaataaga tgctgcctaa
     2941 ggtgttcttc agcgccaaga gtatcaaatt ctacaaccca tccgaggaca tcctgcggat
     3001 tagaaatcac tcaacacata ctaagaacgg gagcccccag aagggatatg agaaatttga
     3061 gttcaacatc gaggattgca ggaagtttat tgacttctac aagcagagca tctccaaaca
     3121 ccctgaatgg aaggattttg gcttccggtt ttccgacaca cagagatata actctatcga
     3181 cgagttctac cgcgaggtgg aaaatcaggg gtataagctg acttttgaga acatttctga
     3241 aagttacatc gacagcgtgg tcaatcaggg aaagctgtac ctgttccaga tctataacaa
     3301 agatttttca gcatacagca agggcagacc aaacctgcat acactgtact ggaaggccct
     3361 gttcgatgag aggaatctgc aggacgtggt ctataaactg aacggagagg ccgaactgtt
     3421 ttaccggaag cagtctattc ctaagaaaat cactcaccca gctaaggagg ccatcgctaa
     3481 caagaacaag gacaatccta agaaagagag cgtgttcgaa tacgatctga ttaaggacaa
     3541 gcggttcacc gaagataagt tctttttcca ttgtccaatc accattaact tcaagtcaag
     3601 cggcgctaac aagttcaacg acgagatcaa tctgctgctg aaggaaaaag caaacgatgt
     3661 gcacatcctg agcattgacc gaggagagcg gcatctggcc tactataccc tggtggatgg
     3721 caaagggaat atcattaagc aggatacatt caacatcatt ggcaatgacc ggatgaaaac
     3781 caactaccac gataaactgg ctgcaatcga gaaggataga gactcagcta ggaaggactg
     3841 gaagaaaatc aacaacatta aggagatgaa ggaaggctat ctgagccagg tggtccatga
     3901 gattgcaaag ctggtcatcg aatacaatgc cattgtggtg ttcgaggatc tgaacttcgg
     3961 ctttaagagg gggcgcttta aggtggaaaa acaggtctat cagaagctgg agaaaatgct
     4021 gatcgaaaag ctgaattacc tggtgtttaa agataacgag ttcgacaaga ccggaggcgt
     4081 cctgagagcc taccagctga cagctccctt tgaaactttc aagaaaatgg gaaaacagac
     4141 aggcatcatc tactatgtgc cagccggatt cacttccaag atctgccccg tgaccggctt
     4201 tgtcaaccag ctgtacccta aatatgagtc agtgagcaag tcccaggaat ttttcagcaa
     4261 gttcgataag atctgttata atctggacaa ggggtacttc gagttttcct tcgattacaa
     4321 gaacttcggc gacaaggccg ctaaggggaa atggaccatt gcctccttcg gatctcgcct
     4381 gatcaacttt cgaaattccg ataaaaacca caattgggac actagggagg tgtacccaac
     4441 caaggagctg gaaaagctgc tgaaagacta ctctatcgag tatggacatg gcgaatgcat
     4501 caaggcagcc atctgtggcg agagtgataa gaaatttttc gccaagctga cctcagtgct
     4561 gaatacaatc ctgcagatgc ggaactcaaa gaccgggaca gaactggact atctgattag
     4621 ccccgtggct gatgtcaacg gaaacttctt cgacagcaga caggcaccca aaaatatgcc
     4681 tcaggatgca gacgccaacg gggcctacca catcgggctg aagggactga tgctgctggg
     4741 ccggatcaag aacaatcagg aggggaagaa gctgaacctg gtcattaaga acgaggaata
     4801 cttcgagttt gtccagaata gaaataacaa aaggccggcg gccacgaaaa aggccggcca
     4861 ggcaaaaaag aaaaagggat cctacccata cgatgttcca gattacgctt atccctacga
     4921 cgtgcctgat tatgcatacc catatgatgt ccccgactat gcctaagaat tctgcagata
     4981 tccagcacag tggcggccgc tcgagtctag agggcccgtt taaacccgct gatcagcctc
     5041 gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac
     5101 cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg
     5161 tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga
     5221 ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga
     5281 aagaaccagc tggggctcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc
     5341 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
     5401 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     5461 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     5521 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     5581 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
     5641 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg
     5701 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt
     5761 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     5821 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     5881 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
     5941 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     6001 tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt
     6061 tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct
     6121 gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt
     6181 tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc
     6241 tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag
     6301 accgacctgt ccggtgccct gaatgaactg caggacgagg cagcgcggct atcgtggctg
     6361 gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac
     6421 tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc
     6481 gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc
     6541 tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc
     6601 ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg
     6661 ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat
     6721 gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc
     6781 cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa
     6841 gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat
     6901 tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg actctggggt
     6961 tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg agatttcgat tccaccgccg
     7021 ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc
     7081 agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata
     7141 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     7201 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga
     7261 cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     7321 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     7381 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     7441 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     7501 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     7561 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     7621 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     7681 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     7741 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     7801 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     7861 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     7921 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     7981 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     8041 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     8101 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     8161 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     8221 gtagcggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     8281 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     8341 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     8401 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     8461 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     8521 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     8581 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     8641 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     8701 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     8761 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     8821 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     8881 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     8941 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     9001 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     9061 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     9121 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     9181 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     9241 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     9301 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     9361 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     9421 cccgaaaagt gccacctgac gtc
//
