LOCUS       sequence-202856-       14745 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Kinase-deleted Ikk1(alpha) expression in basal keratinocytes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14745)
  AUTHORS   Lisse TS, Rieger S
  TITLE     IKKalpha regulates human keratinocyte migration through surveillance
            of the redox environment.
  JOURNAL   J Cell Sci. 2017 Mar 1;130(5):975-988. doi: 10.1242/jcs.197343. Epub
            2017 Jan 25.
  PUBMED    28122935
REFERENCE   2  (bases 1 to 14745)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1242/jcs.197343"; journalName: "J Cell Sci"; date: "2017-03-1- 
            1"; volume: "130"; issue: "5"; pages: "975-988"
COMMENT     Sequence Label: pT2KXIG (Tol2)-tp63:ikk1KD:GFP
FEATURES             Location/Qualifiers
     source          1..14745
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      1384..1393
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1390..2109
                     /codon_start=1
                     /product="Aequorea coerulescens GFP"
                     /label=AcGFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGAELFTGIVPILIELNGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFEDD
                     GNYKSRAEVKFEGDTLVNRIELTGTDFKEDGNILGNKMEYNYNAHNVYIMTDKAKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMIYF
                     GFVTAAAITHGMDELYK"
     polyA_signal    2153..2287
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2177..2196)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2231..2250
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    join(4437..4624,4625..4636)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(4670..4686)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4670..4686)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(4727..4747)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(4727..4745)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4766..4782)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4766..4782)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4779..4801)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4790..4806
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4814..4820,4821..4838,4839..4844))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4859..4880
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4997..5014)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5168..5756)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5248..5267)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5927..6718,6719..6787))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6550..6569
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6788..6892)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(6918..7373)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7055..7076)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7267..7286
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7500..7522
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7514..7531
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7515..7531
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7541..7560
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7541..7559
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    join(9411..9422,9423..9585)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
ORIGIN
        1 atggagaaac cccctttcag acagaatcag gtctgtggct catgggttat gaaggagagg
       61 cttggaacag gaggatttgg cccagatgaa gggcttcact ctctgcaaca gaggattgag
      121 aacgaaacca aaattgaact tttgaatcaa gatcttctgc aagaaacagg agtcatgtta
      181 gatcccagga aacctgctgc acagtgtgtg ttagatggag taagaggatg ggacagctac
      241 attgtatatc tgtttgacaa gagtttgacc aagtacatgg gacctttaac tgctagaaca
      301 cttcccgaaa gtgtcaactt tattgttcgg gaaaccaaaa ctcagctccc actgagtacc
      361 ctaaaaaagg tttggggtga ggcggttagc tacatttgtg gccccagaga agactacagc
      421 cgcctcttcc agggccagcg tgcagccatg ctgagtcttc ttcgcttcaa tacaaatttg
      481 acaagataca agaacatgat gttctccttt tcacaacagc tcaaagcaaa gctggacttt
      541 tttaagacca gcatacagta cgacttggag aaatacagtg atcagatgca gtacggcata
      601 tcttcagaaa agatgctgaa ggcttggcac gaaaatgaag agagggctgc tgcttttgca
      661 caggtggcag agattggtca tctggatgag gaaataatgg cgttgcattc ggaaatcgtt
      721 gagttacaga gaagtcctta tgctcgtcgt caaggagatg tcatggagca gttgcaagag
      781 aaagccgttg aactctacaa acagttgaag gcaaaatgca aaatgccaga ccctcagcat
      841 ggctacagcg acagctctga gatggtgaag gtcatagtac agactgtcca gaatcaagac
      901 cgggtgctaa gagatctata tgcacacctc agtaaaatcc ctctgagtaa gcagaagatc
      961 atcgacctct tccctaaaat cgagagaacg ctggaatgca taaaggaggc agacaccaca
     1021 gtcatgcaga tgcagatcaa gagacagaga gagttctggc acttactgaa gattgcctgt
     1081 gcacaaaaca ccacacggag ctcagtgagt cagagcggag aaatgccctc ctcgctgtct
     1141 acttggaacc agactcaagc ccagtgttcc tcccgtctcc ccatgtcctt gcaagtgcca
     1201 cacgagggtg attcagttaa tcatttactg gaggagaatc agcgttactt gactcagctc
     1261 accagtttac tgcaagaaac caccgaggag aaatcagaga gcataatggc tcaggactgg
     1321 agctggacaa aatacgaatc cctggttgct aaatcagcgc gtttgtatac ggatccaccg
     1381 gtcgccacca tggtgagcaa gggcgccgag ctgttcaccg gcatcgtgcc catcctgatc
     1441 gagctgaatg gcgatgtgaa tggccacaag ttcagcgtga gcggcgaggg cgagggcgat
     1501 gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcctgtgccc
     1561 tggcccaccc tggtgaccac cctgagctac ggcgtgcagt gcttctcacg ctaccccgat
     1621 cacatgaagc agcacgactt cttcaagagc gccatgcctg agggctacat ccaggagcgc
     1681 accatcttct tcgaggatga cggcaactac aagtcgcgcg ccgaggtgaa gttcgagggc
     1741 gataccctgg tgaatcgcat cgagctgacc ggcaccgatt tcaaggagga tggcaacatc
     1801 ctgggcaata agatggagta caactacaac gcccacaatg tgtacatcat gaccgacaag
     1861 gccaagaatg gcatcaaggt gaacttcaag atccgccaca acatcgagga tggcagcgtg
     1921 cagctggccg accactacca gcagaatacc cccatcggcg atggccctgt gctgctgccc
     1981 gataaccact acctgtccac ccagagcgcc ctgtccaagg accccaacga gaagcgcgat
     2041 cacatgatct acttcggctt cgtgaccgcc gccgccatca cccacggcat ggatgagctg
     2101 tacaagtgag cggccgccac cgcggtggag ctcgaattaa ttcatcgatg atgatccaga
     2161 catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg
     2221 ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa
     2281 acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga
     2341 ggttttttaa agcaagtaaa acctctacaa atgtggtatg gctgattatg atcctctaga
     2401 tcagatctgt cccgatcttt ctcttctgtg ctgtcaattt agagtagttt ttgaggtaat
     2461 ttgggtgcat tctctgtaaa aacaacaaat aaatatttat attgtatgca ataaatgtaa
     2521 caattacaca tacactctct gattacagca ttaaagggtt agttcaccca aaaatgaaaa
     2581 gatgtcatta atgactcacc ctcatgtcgt tccaagcccg taacacctcc gttcatcttc
     2641 ggaacacagt ttaagatatt ttagatttag tccgagagct ttctgtgcct ccattgagaa
     2701 tgtatgtacg gtatactgtc catgtccaga aaggtaataa aaacatcaaa gtagtccatg
     2761 tgacatcagt gggttagtta gaattttttg aagcatcgaa tacattttgg tccaaaaata
     2821 acaaaaccta cgactttatt cggcattgta ttctcttccg ggtctgttgt caatccgcgt
     2881 tcacgacttc gcagtgacgc tacaatgctg aataaagtcg taggttttgt tatttttgga
     2941 ccaaaatgta ttttcgatgc ttcaaataat tctacctaac ccactgatgt cacatggact
     3001 actttgatgt ttttattacc tttctggaca tggacagtat accgtacata cattttcagt
     3061 ggagggacag aaagctctcg gactaaatct aaaatatctt aaactgtgtt ccgaagatga
     3121 acggaggtgt tacgggcttg gaacgacatg agggtgagtc attaatgaca ttattttcat
     3181 ttttgggtga actaaccctt taataaaact gacatttgca gccaattgca cactttataa
     3241 tgttaaatac ttgttgattt tagaaataca gtagcattta gtgacaatca aacattaatt
     3301 ttatttctga ttatattgac aaaaacacag gactgtaagg ctgacatgac agggtgaatt
     3361 gatttcatta cattagtttt gatggtaaca cttttacaat aaggttcgtt agttaactac
     3421 attagttaac atgaactaat aatgaactgc acgtatacat gttaatttca acatttacta
     3481 atactttata tcttgttaac attagttaat gcactgtgaa ctaacatgaa caaacaatga
     3541 acatctgtat ttctattaac taacgttaac aaagattaac agatacagta acaaatgtat
     3601 tgctcatggt tatttaatgt tagttaatac atgaactaat caacctcatt gtaaagtgtt
     3661 accgttttga caatacaaca tgtttggaaa aaaatttctc gcttggtttt cttgtgtttt
     3721 gcatgaattc aaacacaatg agagcacgga atgataatga ttattcaaag ctgcaatagg
     3781 cgccaaattt cccgtggacc taacgttacc aattacaaat gcattttagc attttattac
     3841 gtgagcctta tattgtcaat cgcatgcatt agtgaaaata ggcctactac acatataaaa
     3901 ggcgcgccat caacgtcaaa acatgcacac ccnnaaaaaa aaaaaaaaaa aaaaaaaaaa
     3961 ctatcagtaa aacaaaatac ttaatgtact tacctcaata tgcttcctta ggtttgatgg
     4021 cgaacttttg aaggccgata tttctttatt aagcgggaga cagaggacac atttcatctt
     4081 gaatgaatct ttatttacac cacttaaaga aaagaattcg cgaagatacg gccacgggtg
     4141 ctcttgatcc tgtggctgat tttggactgt gctgctcgca gctgctgatg aatcacatac
     4201 ttcctccatt ttcttccact gattgactgt tataatttcc ctaatttcca ggtcaaggtg
     4261 ctgtgcattg tggtaataga tgtgacatga cgtcacttcc aaaggaccaa tgaacatgtc
     4321 tgaccaattt catataatgt gaaaacgatt ttcataggca gaataaataa catttaaatt
     4381 aaactgggca tcagcgcaat tcaattggtt tggtaatagc aagggaaaat agaatgaagt
     4441 gatctccaaa aaataagtac tttttgactg taaataaaat tgtaaggagt aaaaagtact
     4501 tttttttcta aaaaaatgta attaagtaaa agtaaaagta ttgattttta attgtactca
     4561 agtaaagtaa aaatccccaa aaataatact taagtacagt aatcaagtaa aattactcaa
     4621 gtactttaca cctctggttc ttgaccccct accttcagca agcccagcag atccactagt
     4681 tctagagcgg ccggccgcca ccgcggtgga gctccagctt ttgttccctt tagtgagggt
     4741 taattgcgcg cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     4801 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     4861 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     4921 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     4981 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     5041 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     5101 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     5161 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     5221 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     5281 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     5341 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     5401 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     5461 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     5521 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     5581 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     5641 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     5701 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     5761 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     5821 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     5881 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     5941 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     6001 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     6061 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     6121 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     6181 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     6241 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     6301 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     6361 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     6421 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     6481 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     6541 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     6601 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     6661 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     6721 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     6781 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     6841 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     6901 cccgaaaagt gccacctaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt
     6961 ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc
     7021 aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt
     7081 aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact
     7141 acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg
     7201 gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag
     7261 aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac
     7321 gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtcccattc
     7381 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg
     7441 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc
     7501 ccagtcacga cgttgtaaaa cgacggccag tgagcgcgcg taatacgact cactataggg
     7561 cgaattgggt accaaatagt aggaattacc cacctgtaca agtgctgaaa acttggatga
     7621 ataagcccgt ttgccatttt ctactgctat tttaaatctt ttctgtattt gtctgcattt
     7681 gtctttaccc ttgaataaat gttttagttg ttttttttca attatgactg tgtttaacag
     7741 acattattac aataatatgt aatagtagta cacactatta ttatgtaata gtacttgttg
     7801 actgtatttg agaactggac cgagtgagtg ttacgtcacc cattcaaaat gacttacttc
     7861 tggctccaac aaaatgaagt taattcagtt gccatttttc actgtatgga catcgccgtg
     7921 ttggagctag acgttgccaa taagcaagaa agagccgaga tgcgtcgagt ctgagtcacc
     7981 gttcctatgg caacccctct aaccaatcag aagtaagctt gttggaagtc cacagcctac
     8041 cacttgaaag cgggctgcac aaaatctgtc aaacgttttg aacgttggat gtgagagcac
     8101 atacttttat taaggcatct ggttggtcag tttataatat caacaacttg ggctacagaa
     8161 aagaaaagtt attacagaaa ttatgattaa caagtacatg ttaaataaag attttaatat
     8221 gaatgccacc actggagcat tcatgccatt tggagcttct tcctgtttgg atcactagaa
     8281 ggaggaggtc actcattaca gttctcatat acagtcgttg gttggttggt tggttggtag
     8341 attgattgat tgattgattg attgattgat tgattgattg attgattgat tgattgattg
     8401 attgattgat tgattggtag tcaaaataag aaataatttc cacagattca ttacagaaat
     8461 gattaaatgc atacataaaa aactnggggg ggggggggat acaacaacac acttaagtca
     8521 catttgccta cgtaaagaaa agtaaagaaa atcaatagct atattttaca tctccttttt
     8581 ttgctgtctt ttaattagcc ttgttttgct gttatcttga ttgaaactgt aacttcttca
     8641 cctgcttctt ttctttgtag gttttgccag cccagaaaca ggcatggctg tgcaaggcca
     8701 gagcaccatg cacaatgcgc tgcatgtctt catgaacggc tcaatgtcct cagtccaggg
     8761 ctcagccaac gaccccatct tcctccttca ccatgctttc attgacaggt aacaaacacg
     8821 tcatgacatt agactgcaca gtttttgaca aagttcatac aatctgttgt ttatagctgc
     8881 tacaattagt gaagtttgtg aatgtacttg gatgagcagc gaaagatcaa ttgagatcaa
     8941 ttgttagagt ttggttgccc tgcagagcaa agaacaaaaa ataatctggt ggctttactg
     9001 cgtgaggtta ttattggtgg aatagaaaca caaaacataa ttgcatttat ttgtttaatt
     9061 ttttatctta tcttaacttt catcttgcat atttgtttct tacatcattt ctagcatctt
     9121 tgagcgctgg ctaagaactc atcagcctcc ccggtccatc tacccacgta ccaatgcacc
     9181 aattggccac aatgacggct actacatggt gccattcctt cctctttata ggaatggaga
     9241 ctacctcctg tccaacaatg ctcttggata cgagtacgcc tacctgttgg acccaggtca
     9301 ttgcacaaca ccagaaatgc cctctgatct gcaaaagacg tgaatatctg ttcagacacc
     9361 catatccact ctgttccaca caggtcagag gtttgtccag gagttcttga cagaggtgta
     9421 aaaagtactc aaaaatttta ctcaagtgaa agtacaagta cttagggaaa attttactca
     9481 attaaaagta aaagtatctg gctagaatct tacttgagta aaagtaaaaa agtactccat
     9541 taaaattgta cttgagtatt aaggaagtaa aagtaaaagc aagaaagaaa actagagatt
     9601 cttgtttaag cttttaatct caaaaaacat taaatgaaat gcatacaagg ttttatcctg
     9661 ctttagaact gtttgtattt aattatcaaa ctataagaca gacaatctaa tgccagtaca
     9721 cgctactcaa agttgtaaaa cctcagattt aacttcagta gaagctgatt ctcaaaattg
     9781 ttagtgtcaa gcctagctct tttggggctg aaaagcaatc ctgcagtgct gaaaagcctc
     9841 tcacaggcag ccgatgcggg aagaggtgta ttagtcttga tagagaggct gcaaatagca
     9901 ggaaacgtga gcagagactc cctggtgtct gaaacacagg ccagatgggc cctcgagaga
     9961 aaccaggctc agtcgggcac gaccattact ccacttgacc aaacgtctgc atatttataa
    10021 ataataattt gcgtattaat atgcagataa agacaattaa aagaagcaac agtagcatta
    10081 atcattcaaa taacagtata caatcaaaca acattttgta tatatattta aaaaaacaat
    10141 acaaggagta aagacaaagg caatatcatt tagttttttt aatattaaca aaccacaggc
    10201 tgtgtgaacc atcagcaatt tcaaaccaga gggatctgct tttttgggaa cgatgttgca
    10261 aatttaatct ctagattttt taaatcatgg atagcctccc taaagcaaaa gctttttagg
    10321 ggctttactt ctcctgattg cagcctgtgg tataatttgt gattgttagg tgggtcaggt
    10381 tttggggtcc agccctttgt gcactttgac tattgtcacc ttaggctgtc ccttaatgct
    10441 gccacctctg accccggtgg gacacataaa taacttctcc acccgagtta gcatttcaag
    10501 ccagtgcaat aaagaaatta aaaataattg atttattaac aactctgttt tatctttaaa
    10561 aaataaaatg ttttatatct aatgttcact caggtacctg atattgtttg ctgtgcaata
    10621 atgtaggtta gtcattatat tgacaaagaa aattaaatat gatgttgttt atttttaatt
    10681 ttacattata gactagatta gattttcatt tggaatttaa ttaggagtgc ctggagtgca
    10741 ttgagaacgt taaaaaaaaa aaatctgctt actcttatag ctttggaaac tattttcaga
    10801 ttattactcc actatagaag cgattccttt ttttgaataa ttaaatatat atatacagtg
    10861 ctcagcataa atgatcatca cagatctcta ttttaaagta ttatttttcc ataggatgct
    10921 ttacaataat atatgtgtgt gtatacatta gaattgtcag ttacaaaatc aaaactgcaa
    10981 caaaataact aaacttaaga tctaatgagt acacccaaat taaaccaaac attagtctat
    11041 gcaaaaatta atatgttaag gaaaaatatt aaatacaatt ttaatattca gtcattcatt
    11101 ttcttttttg cttagtccct ttattaatct ggggtcgcca cagtggaatt aactgccaat
    11161 ttatccagca tatattttat gcagcggatg ctcttccagc tgctacccat cactgggaat
    11221 cacccataca ctctcattca cacacataca ctacggacaa tttagcctac ccaattcacc
    11281 tatagcgtat gtctttggac ttgtggggga aaccggaaca cacggaggaa acccacgcga
    11341 acacagggag atcatgcaaa ctcagaaacg ccaactgatc cagccaaggc tcgaaccagt
    11401 gaccttcttg ctgtgaggca atagggctac ccattgtgtc actgcaccgt ccacaatttt
    11461 aataaacatg aaaaatcccg agaaacataa acaatattaa atgtagttga aattttgttg
    11521 tttgaaattg tatttattaa ataaaaaaaa tgtatgtatt attattattt atttatttat
    11581 aaaagatgtt aggtggccaa actctttttt ttaatggata tagttgttta ataaaacaat
    11641 tttgtttaaa taaaacaaaa tatattgaat gagccatgtt gctgttgttg ttttttacag
    11701 gcttgatgta caatatgatc tttgtgtagt tttatagtaa taaatcattc atgtcttgtg
    11761 tattgtacgt catattatct taaaagtacg ctattaatta aacttgttta tatggtcatc
    11821 ttagacagga ctctcattat aaaaggattt ttgttaattt tattagtacc ttcctggaaa
    11881 aataaaagat aaataaataa aaatgaataa aagtcttggg cttgttttct gtacgagtaa
    11941 taaaaaagag taagcaaata ttctgcatga caatattgtt caaagtttaa gggctgtatt
    12001 ttttataatt gttggttatt aacatgtttt atacaaatta ctttcaacat ggctgaaata
    12061 ataattgtta tcactatttg aaacagtcct agaatagatt ttactatatt tacatttatt
    12121 cctctgatga caaagttgaa attcctctga tgacaaagca aaactctcaa ctctagtttt
    12181 attttattgt aataaccatg tcaagggata atgacaatgt tttccattat attttcaata
    12241 tagaataaaa ttataattta ttaatcattt agtagggcag catggtggca caatgggtag
    12301 cacaatcacc tcacaccaag aaggttgcta gttcgagccc cggctgggtc agtttgcata
    12361 tctgtgtgga gaatgttctc tctgtgttgc catgggtttc ctctgggtgc tctagtttcc
    12421 accacaagtc taaaaacaag tggcatcggt gaattgtgta ggctaaattg tctgtagtgt
    12481 ttgtgtgtga ttgagtgttt atggatgttt cccagtgatg ggttgcagtt ggaagggcat
    12541 tctctgcgta aaactataag ttggcggttc attccactgt ggtgacccca gattaataaa
    12601 gggattaagc cgaaaagaaa atgaatcatt aaatgatcat ttagttgatt ggagagcatt
    12661 gcataatgag aatcaaataa tgtatagttt tttgtaaaat gattgtgtat tagaggcaca
    12721 ttgtacgtag tgtggaatgt ttaggttgtc tttctgattg ttttcatttt gcagaagttc
    12781 atttgcagtc agtgtagtca aagcacctca gctcaattta tagcagcagc ctgctgtgat
    12841 ttaagtgcca caggagtgaa ggctttaacc cctgttgtag attgcaacca atgtttttct
    12901 aaggtaatta gaagcttgtt tagctaacgc tacccacccc accccccaca aaaaagttgc
    12961 tctgaatagc agcatctacc cccaggaggg tgcaagcttc tgttttgaag aaatttgctc
    13021 atggtgttac ataggtgttt aaaaatgtat gttttttaat agcatatatt ttaaaagtga
    13081 ctcattaatg acatcatctg ccatctgcct ttctcagaac tgacaaagga cccttttaag
    13141 gacccctata caccacattt ttatctatct aatcacaagt gggtgatatt agagggatga
    13201 ttcacctaaa aatgaatttt ccgcatttta atttggggtt ccaaaccagt ttgaattctt
    13261 ttgttctgtt gaacacaaaa taagatgttt taagaatgtt ggaaatgttg gagactttta
    13321 tagtaggaaa aacatatact aatatttgca ttcatgtttg aaatgtttaa aacatttttc
    13381 aaaatatctt atttttcaac tgttttggaa caagtcaaaa gtgagtaaat gatgactgat
    13441 aaacaatttc tttaaataca gatgaaaata gcgtctaaca cattttgaac tagttcactt
    13501 tcaaccacag caagaggtag tcgaaaacac atttgatcac attttgttgt gggaccgctt
    13561 gttgttcagt gtgttttcat tgttgagtta caaaccggta agtggcatta aaacatgttt
    13621 tggcatcact gatgatcata aataaattgg ttaaaaaatt aaaaattaga tgcttacagt
    13681 acagtttgtt taaaaacttg cactttttgt agtgtgaaat gacattagta cctgcagtgg
    13741 gctttctttg ttctgtatct tctgatgaat ctaacataaa gacatttaaa tattcacaat
    13801 ggagtgggtt ggttctcatc tatctgaaac aacattagtg tgttcaaaac aaatctacac
    13861 cagtgatctc tgatagatac aagctgtcaa ccgtagtcct attgtttctc atgcgactgt
    13921 cacagggcca tgctagaggc tctttgggtg gtgagtagag actcattctt atgcaaacag
    13981 gctatggatg ctacaactga agagaattta gtgctctaag gtcatcgcag ttctataaag
    14041 cagattccct ccaaaaagct agagaaaatt atgtgtctgg aggcaccatc tatcagtgca
    14101 gtcattcttt tattactttt tttttttgtc catgcaattt tttaatcgca ttttgaagta
    14161 tttaaaccat taagagtgtc aaattatcat gttattattt caactcataa tatctccttt
    14221 taaagatgat tcatttgatt gtagttataa taattgtttt attaatgcaa aaattgctta
    14281 aatgtttggc ctctgagatt ttcagttgac tcaatatttt tggagcctgt ttcatgactt
    14341 tatttaaaat ataatctgca ctcgcaaata ttacaattat atcatatttt gaaaatctta
    14401 gatgtacatt tgttttgaaa atggtttaaa ggaaatttag gtcaaaatgt gtgttttttt
    14461 taaataataa ttcagtcttt tttttgatat aatagggaaa atgacagcaa ttaaaaggct
    14521 gttttggaaa gacatctagg tctggtgcag acttcgtaac ttcttagagt taaatcttga
    14581 aaacggagcc agaagacggg caacaccttc ctaaaagcag ggtggggtgt tggacaggta
    14641 gcttcttgga acctcccctc tttgtcgctg tggtatatat agaaatccca ttgaattcct
    14701 atagagacct taagcccgta ccatttacct ctgaaggatc ctaat
//
