LOCUS       sequence-202857-        9739 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Co-expression of tdTomato and HyPer in differentiated keratinocytes 
            of the periderm layer and ealy on also in the basal layer of the 
            epidermis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 9739)
  AUTHORS   Lisse TS, Middleton LJ, Pellegrini AD, Martin PB, Spaulding EL, 
            Lopes O, Brochu EA, Carter EV, Waldron A, Rieger S
  TITLE     Paclitaxel-induced epithelial damage and ectopic MMP-13 expression 
            promotes neurotoxicity in zebrafish.
  JOURNAL   Proc Natl Acad Sci U S A. 2016 Apr 12;113(15):E2189-98. doi: 
            10.1073/pnas.1525096113. Epub 2016 Mar 28.
  PUBMED    27035978
REFERENCE   2  (bases 1 to 9739)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1525096113"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2016-04-12- 12"; volume: "113"; issue: "15"; pages: 
            "E2189-98"
COMMENT     Sequence Label: 
            pDEST-Tol2(Chien)-Krt4:Gal4VP16:tdTomato:5xUAS:HyPer:pA
FEATURES             Location/Qualifiers
     source          1..9739
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..702
                     /codon_start=1
                     /product="dimeric variant of DsRed fluorescent protein 
                     (Shaner et al., 2004)"
                     /label=dTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLYGMDELYK"
     primer_bind     complement(134..152)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     402..421
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     596..619
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     polyA_signal    827..961
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(1234..1674)
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     misc_feature    join(4253..4415,4416..4427)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     4776..4797
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4960..5064
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5065..5133,5134..5925)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5283..5302)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6096..6684
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6585..6604
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6838..6855
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6972..6993
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7008..7013,7014..7031,7032..7038)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7046..7062
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7051..7073
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7070..7086
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7070..7086
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7105..7125
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7107..7125
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     7162..7178
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7162..7178
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     misc_feature    join(7212..7223,7224..7411)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     polyA_signal    7850..7984
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(8004..9440)
                     /codon_start=1
                     /product="improved fluorescent indicator for intracellular 
                     H2O2 (Bilan et al., 2013)"
                     /label=HyPer-3
                     /translation="MEMASQQGETMSGPLHIGLIPTVGPYLLPHIIPMLHQTFPKLEMY
                     LHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPLFDEPMLLAIYEDHPWANRECVP
                     MADLAGEKLLMLEDGHCLRDQAMSAGYNSDNVYIMADKQKNGIKANFKIRHNVEDGSVQ
                     LADHYQQNTPIGDGPVLLPDNHYLSFQSVLSKDPNEKRDHMVLLEFVTAAGITLGMDEL
                     YNVDGGSGGTGSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKLICT
                     TGKLPVPWPTLVTTLGYGLKCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKT
                     RAEVKFEGDTLVNRIELKGIGFKEDGNILGHKLEYNGTGFCFEAGADEDTHFRATSLET
                     LRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGSPLRSRYEQL
                     AEAIRARMDGHFDKVLKQAV"
     primer_bind     8422..8441
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8681..8702
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(8811..8832)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 atggtgagca agggcgagga ggtcatcaaa gagttcatgc gcttcaaggt gcgcatggag
       61 ggctccatga acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag
      121 ggcacccaga ccgccaagct gaaggtgacc aagggcggcc ccctgccctt cgcctgggac
      181 atcctgtccc cccagttcat gtacggctcc aaggcgtacg tgaagcaccc cgccgacatc
      241 cccgattaca agaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc
      301 gaggacggcg gtctggtgac cgtgacccag gactcctccc tgcaggacgg cacgctgatc
      361 tacaaggtga agatgcgcgg caccaacttc ccccccgacg gccccgtaat gcagaagaag
      421 accatgggct gggaggcctc caccgagcgc ctgtaccccc gcgacggcgt gctgaagggc
      481 gagatccacc aggccctgaa gctgaaggac ggcggccact acctggtgga gttcaagacc
      541 atctacatgg ccaagaagcc cgtgcaactg cccggctact actacgtgga caccaagctg
      601 gacatcacct cccacaacga ggactacacc atcgtggaac agtacgagcg ctccgagggc
      661 cgccaccacc tgttcctgta cggcatggac gagctgtaca agtaggcggc cgcgactcta
      721 gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca
      781 cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc
      841 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
      901 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat
      961 ctacgtaata cgactcacta tagttctaga ggctcgagag gggccgcact agtagatccg
     1021 ctacatatcc agagcgccgt agggggcgga gtcgtggggg gtaaatcccg gacccgggga
     1081 atccccgtcc cccaacatgt ccagatcgaa atcgtctagc gcgtcggcat gcgccatcgc
     1141 cacgtcctcg ccgtctaagt ggagctcgtc ccccaggctg acatcggtcg ggggggcggc
     1201 tcgagagatc tgaattcccg gggtcgacct cgacgataca gtcaactgtc tttgaccttt
     1261 gttactactc tcttccgatg atgatgtcgc acttattcta tgctgtctca atgttagagg
     1321 catatcagtc tccactgaag ccaatctatc tgtgacggca tctttattca cattatcttg
     1381 tacaaataat cctgttaaca atgcttttat atcctgtaaa gaatccattt tcaaaatcat
     1441 gtcaaggtct tctcgaggaa aaatcagtag aaatagctgt tccagtcttt ctagccttga
     1501 ttccacttct gtcagatgtg ccctagtcag cggagacctt ttggttttgg gagagtagcg
     1561 acactcccag ttgttcttca gacacttggc gcacttcggt ttttctttgg agcacttgag
     1621 ctttttaagt cggcaaatat cgcatgcttg ttcgatagaa gacagtagct tcatcctagg
     1681 cctctgcgtg tctctcagca gctggctgac caattttata cacactcttg agggggtgga
     1741 gctgactgct ctcacacaca cacttgcaca catacgcact ctcacacaca ccctacctgt
     1801 tagacaggta cagagagaaa gtggtaagat aaatggtgtc acacccagcc tggggctttg
     1861 ccccaggtgg aatgagaaaa tgcaaggaga ggcttgagac ttgaggacag gcattccagt
     1921 agaaactctg aaaaagacag tgtagctctc accatctgca agcgggtgtg tttctgcctc
     1981 agtatctggt tggctggaca gccgtagaga gcatgttaac ctctgctctt tttatctggg
     2041 tggggcgcgc agcaactctt acattaatga aaggcttttt tccatccact tttgccagcg
     2101 tttggatctg gcagggcatt tttccccctg agctgaacag ttgcattgtt gagatttaca
     2161 agggaacagg gaacagagtt tccttctgta ttgatattgg aaaatgtgca cacacacaat
     2221 tctgcacagt aatgctagcg gctaacaaag ctaatgtagt ttatggctct aaactccacc
     2281 atatgtgtaa acatatgatt atgtatgggg agggctgcag tagtggaaag agtactgaaa
     2341 aatcatagta aaaataccat tacatgccta aaaatccagt gcgagtaaaa gtatctgttg
     2401 taactgttac tcaaagtatg agtaaaaagt agctttttta aaagtactca agagtagtaa
     2461 gtagtgagta ttatgcctta aaaagctaat gcatttacat gtaatttaca tgtgtgtaaa
     2521 cgtaacattc tgtagtgcat ttagttattg cccagcaggc acacaacatc ataagatgtt
     2581 aatattaggt taaatatagg ttgtggtgac ctaaattcaa agtctagcca gcatctaagg
     2641 atattgttat tttgatgtcc aataacgatg tcataggaag ttaatatttg gtggatttag
     2701 gttgtgttgg aaagcgacat aagtccaatg tcgaaccaac atcttaaaca agtgtaaaat
     2761 aatgacattt attcattagg tatggcaacc ataatccaac ttctgataga catcataggg
     2821 gtaacatcca cacaacatca agctgtacat cattagacat tgatatatga ttttagattg
     2881 gatgttgaac cttgacatcg acctgacgtt gagctctgac gtcaacctaa ttttcatttc
     2941 taaacaaaat acaacgtccc cattgcaatg gggtacaacg taaacctata gccatgttga
     3001 cgtcttgtgc cgtcttgtgt gcgtttaagg ttatttcggt catcatacag aaaacatccg
     3061 tcatcttctc atcagtgaca tgcatctaag cagtctctgg gttaatgcgg gtaaagattt
     3121 ttgacatctt cttttaattc tggcaaacag tttgctgcaa ttataaatgg cccatgtctt
     3181 gaggaagttc attatgatgt gatttacctt ctatgtgcga tttgattgga caggaatcac
     3241 aggactgatt tgtctaatcc acatagacaa gaaaaaataa agtagtgact gcaggttgaa
     3301 ggaaagtagt gaaataaaag tacaaatact gcactaaaaa tgtactcaag ggaaagtaaa
     3361 agttgctcta actttaaacc atagtattta atgaccactg ataagttccc acgcatagta
     3421 caccctgcat agtcagacaa tttctcattt ctcgtgcaag tcaacacgaa atggcagcag
     3481 ttgggtgtca cagccagcac tgatgggcat ttaatagcat tacgcaatcg tctacaacta
     3541 tcatagtata aaccaggtgt gaaaggtgtg agattatggt acagcaatca tcactttgta
     3601 tgggtgtttg agatgagtaa taagctctaa atttgctttc atgcacaagc tcttaaaagg
     3661 acgtcagaag ttgaagggaa ggaaaggcaa gaaaatgcat gtccgggctg gatctccggg
     3721 acattgtgga gaagctttac tgtagcaaac tattacacta atcagacagg gcttggccag
     3781 gggtttggcc agtcagaagg aaggtgcaca agagttttgg ggcattattt atttgttatt
     3841 atgcagtaaa tgcatgtttt atgtacttaa agtttacctg ctcgagggcc catctggcct
     3901 gtgtttcaga caccagggag tctctgctca cgtttcctgc tatttgcagc ctctctatca
     3961 agactaatac acctcttccc gcatcggctg cctgtgagag gcttttcagc actgcaggat
     4021 tgcttttcag ccccaaaaga gctaggcttg acactaacaa ttttgagaat cagcttctac
     4081 tgaagttaaa tctgaggttt tacaactttg agtagcgtgt actggcatta gattgtctgt
     4141 cttatagttt gataattaaa tacaaacagt tctaaagcag gataaaacct tgtatgcatt
     4201 tcatttaatg ttttttgaga ttaaaagctt aaacaagaat ctctagtttt ctttcttgct
     4261 tttactttta cttccttaat actcaagtac aattttaatg gagtactttt ttacttttac
     4321 tcaagtaaga ttctagccag atacttttac ttttaattga gtaaaatttt ccctaagtac
     4381 ttgtactttc acttgagtaa aatttttgag tactttttac acctctgtca agaactcctg
     4441 gacaaacctc tgacctgtgt ggaacagagt ggatatgggt gtctgaacag atattcacgt
     4501 cttttgcaga tcagagggca tttctggtgt tgtgcaatga cctgggtcca acaggtaggc
     4561 gtactcgtat ccaagagcat tgttggacag gaggtagtct ccattcctat aaagaggaag
     4621 gaatggcacc atgtagtagc cgtcattgtg gccaattggt gcattggtac gtgggtagat
     4681 ggaccgggga ggctgatgag ttcttagcca gcgtgggcca tcgccctgat agacggtttt
     4741 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
     4801 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc
     4861 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt
     4921 aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     4981 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     5041 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     5101 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     5161 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     5221 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     5281 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
     5341 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     5401 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     5461 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     5521 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     5581 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
     5641 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     5701 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     5761 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     5821 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     5881 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     5941 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     6001 aagatccttt ttgataatct catgaccaaa atgccttaac gtgagttttc gttccactga
     6061 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     6121 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     6181 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     6241 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     6301 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     6361 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     6421 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     6481 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     6541 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     6601 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     6661 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     6721 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     6781 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     6841 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt
     6901 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag
     6961 cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg
     7021 cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc
     7081 tatgaccatg attacgccaa gcgcgcaatt aaccctcact aaagggaaca aaagctggag
     7141 ctccaccgcg gtggcggccg ctctagaact agtggatctg ctgggcttgc tgaaggtagg
     7201 gggtcaagaa ccagaggtgt aaagtacttg agtaatttta cttgattact gtacttaagt
     7261 attatttttg gggattttta ctttacttga gtacaattaa aaatcaatac ttttactttt
     7321 acttaattac atttttttag aaaaaaaagt actttttact ccttacaatt ttatttacag
     7381 tcaaaaagta cttatttttt ggagatcact tcattctatt ttcccttgct attaccaaac
     7441 caattgaatt gcgctgatgc ccagtttaat ttaaatgtta tttattctgc ctatgaaaat
     7501 cgttttcaca ttatatgaaa ttggtcagac atgttcattg gtcctttgga agtgacgtca
     7561 tgtcacatct attaccacaa tgcacagcac cttgacctgg aaattaggga aattataaca
     7621 gtcaatcagt ggaagaaaat ggaggaagta tgtgattcat cagcagctgc gagcagcaca
     7681 gtccaaaatc agccacagga tcaagagcac ccgtggccgt atcttcgcag atctgatcta
     7741 gaggatcata atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc
     7801 acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat
     7861 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     7921 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     7981 gatcatcatc gatggtacat ctattaaacc gcctgtttta aaactttatc gaaatggcca
     8041 tccattcttg cgcggatggc ctctgccagc tgctcatagc ggctgcgcag cggtgagcca
     8101 ggacgataaa ccaggccaat agtgcggcgt ggttccggct taatgcacgg cagataaaca
     8161 accccatcgc gtttgcgctc cggcggcaca gccagcgctg gcagtaaagt gatcccgcta
     8221 cctgccgcca ccatgttgcg cagagtttcc aggctggtcg cgcggaagtg tgtatcttca
     8281 tccgccccgg cttcaaaaca gaaaccggtg ccgttgtact ccagcttgtg ccccaggatg
     8341 ttgccgtcct ccttgaagcc gatgcccttc agctcgatgc ggttcaccag ggtgtcgccc
     8401 tcgaacttca cctcggcgcg ggtcttgtag ttgccgtcgt ccttgaagaa gatggtgcgc
     8461 tcctggacgt agccttcggg catggcggac ttgaagaagt cgtgctgctt catgtggtcg
     8521 gggtagcggg cgaagcactt caggccgtag ccgagggtgg tcacgagggt gggccagggc
     8581 acgggcagct tgccggtggt gcagatcagc ttcagggtca gcttgccgta ggtggcatcg
     8641 ccctcgccct cgccggacac gctgaacttg tggccgttta cgtcgccgtc cagctcgacc
     8701 aggatgggca ccaccccggt gaacagctcc tcgcccttgc tgccggtgcc accgctaccg
     8761 ccatccacgt tgtacagctc gtccatgccg agagtgatcc cggcggcggt cacgaactcc
     8821 agcaggacca tgtgatcgcg cttctcgttg gggtctttgc tcaggacgga ctggaagctc
     8881 aggtagtggt tgtcgggcag cagcacgggg ccgtcgccga tgggggtgtt ctgctggtag
     8941 tggtcggcga gctgcacgct gccgtcctcg acgttgtggc ggatcttgaa gttggccttg
     9001 atgccgttct tctgcttgtc ggccatgata tagacgttgt cgctgttgta gccggcggac
     9061 attgcctgat cgcgcaaaca gtgaccatct tccagcatca gcagtttttc ccctgccaga
     9121 tcggccatcg gtacgcattc gcggttcgcc cacgggtgat cttcatagat agccagcaac
     9181 attggctcat caaacaacgg cacttcaatg aatgcttcgc tctctttcac cagcgcgagg
     9241 atcacgcaat cgagtttgcc gctgtccagt tgcgccagta actggtgggt ctgtgcttca
     9301 tgcagataca tttccagctt tggaaaggtc tggtgcagca tagggataat atgcggtagc
     9361 aggtacggtc caactgtggg aatcaaacca atgtgcagcg gtccggacat cgtctcgccc
     9421 tgctggcttg ccatctccat tacttaaggt cttcgaggtc gagggaattc gtgtggagga
     9481 gctcaaagtg aggctgagac gcgatgggat ccattatata ccctctagac ggaggacagt
     9541 actccgcctt gcgtcggagg acagtactcc gacctctagt gtcggaggac agtactccga
     9601 ccttgccgga ggacagtact ccgccagccc ggaggacagt actccgggcc aaagaattcg
     9661 tgtggaggag ctcaaagtga ggctgagacg cgatgggatc cattatatac cctctagacg
     9721 ggatccaccg gtcgccacc
//
