LOCUS       sequence-202864-       15653 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of Ikk1(alpha) with mutated cysteine residues at position
            179 in basal keratinocytes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15653)
  AUTHORS   Lisse TS, Rieger S
  TITLE     IKKalpha regulates human keratinocyte migration through surveillance
            of the redox environment.
  JOURNAL   J Cell Sci. 2017 Mar 1;130(5):975-988. doi: 10.1242/jcs.197343. Epub
            2017 Jan 25.
  PUBMED    28122935
REFERENCE   2  (bases 1 to 15653)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1242/jcs.197343"; journalName: "J Cell Sci"; date: "2017-03-1- 
            1"; volume: "130"; issue: "5"; pages: "975-988"
COMMENT     Sequence Label: pT2KXIG (Tol2)-tp63:ikk1C179A:GFP
FEATURES             Location/Qualifiers
     source          1..15653
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      2293..2302
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2299..3018
                     /codon_start=1
                     /product="Aequorea coerulescens GFP"
                     /label=AcGFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGAELFTGIVPILIELNGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFEDD
                     GNYKSRAEVKFEGDTLVNRIELTGTDFKEDGNILGNKMEYNYNAHNVYIMTDKAKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMIYF
                     GFVTAAAITHGMDELYK"
     polyA_signal    3062..3196
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3086..3105)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3140..3159
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    join(5345..5532,5533..5544)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(5578..5594)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5578..5594)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(5635..5655)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(5635..5653)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5674..5690)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5674..5690)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5687..5709)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5698..5714
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5722..5728,5729..5746,5747..5752))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5767..5788
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5905..5922)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6076..6664)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6156..6175)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6835..7626,7627..7695))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7458..7477
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7696..7800)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(7826..8281)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7963..7984)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8175..8194
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8408..8430
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8422..8439
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8423..8439
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8449..8468
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        8449..8467
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    join(10319..10330,10331..10493)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
ORIGIN
        1 atggagaaac cccctttcag acagaatcag gtctgtggct catgggttat gaaggagagg
       61 cttggaacag gaggatttgg ccatgtctat ttgtatcaga atcaggaaac atcagaaaaa
      121 atagctgtga agctctgtcg ccttgagctt aattcaaaaa acaaagacag atggagtcga
      181 gagatccaga ttatgaaaaa gctaaagcac cttaatgtgg tcacagcaaa ggatgtgcct
      241 gaggagatga tgcacattgc cttgaatgat ttaccacttt tagccatgga gtattgttcc
      301 aaaggagatc ttcgtaagtt gttgagcaaa cctgaaaatt gctgtgggtt gaaagagagt
      361 gaggtgttgt ctctactcaa tgatgttggt tctggaatcc agtaccttca tgagaataag
      421 atcatacaca gggatctcaa gccagagaat attgtgctgc aagaaattaa tggaaagctt
      481 gttcacaaga taatcgacct tggttatgcc aaagacctgg accaggggag cttagccact
      541 tcatttgtgg ggactttaca atatctggct ccagagcttt ttgaaggcaa gtcctacaca
      601 gtcactgtgg acttttggag ctttggcacc atgatctttg aatgctgctg tggatttcgt
      661 ccatttcttc acaatcttca gcctgtgcag tggacaagca aagtgcgaaa taaaggtccg
      721 aaggatataa tggctgtgga agatatgaat ggcgaggtcc gcttttccac ccaccttcca
      781 tacccaaaca atcttagcag gacactactg gagcccttgg aaggcctttt acagctgatg
      841 ctcaaatggg accctgtaca gagaggatta ggactgaaca cagattcaaa acagccccag
      901 tgctttgttc ttctagatca aatactgagc atgaaggttg tgcacatctt gaacatgact
      961 acgacccagg ttcactcatt tttgctaagc ccagatgaag ggcttcactc tctgcaacag
     1021 aggattgaga acgaaaccaa aattgaactt ttgaatcaag atcttctgca agaaacagga
     1081 gtcatgttag atcccaggaa acctgctgca cagtgtgtgt tagatggagt aagaggatgg
     1141 gacagctaca ttgtatatct gtttgacaag agtttgacca agtacatggg acctttaact
     1201 gctagaacac ttcccgaaag tgtcaacttt attgttcggg aaaccaaaac tcagctccca
     1261 ctgagtaccc taaaaaaggt ttggggtgag gcggttagct acatttgtgg ccccagagaa
     1321 gactacagcc gcctcttcca gggccagcgt gcagccatgc tgagtcttct tcgcttcaat
     1381 acaaatttga caagatacaa gaacatgatg ttctcctttt cacaacagct caaagcaaag
     1441 ctggactttt ttaagaccag catacagtac gacttggaga aatacagtga tcagatgcag
     1501 tacggcatat cttcagaaaa gatgctgaag gcttggcacg aaaatgaaga gagggctgct
     1561 gcttttgcac aggtggcaga gattggtcat ctggatgagg aaataatggc gttgcattcg
     1621 gaaatcgttg agttacagag aagtccttat gctcgtcgtc aaggagatgt catggagcag
     1681 ttgcaagaga aagccattga actctacaaa cagttgaagg caaaatgcaa aatgccagac
     1741 cctcagcatg gctacagcga cagctctgag atggtgaagg tcatagtaca gactgtccag
     1801 aatcaagacc gggtgctaag agatctatat gcacacctca gtaaaatccc tctgagtaag
     1861 cagaagatca tcgacctctt ccctaaaatc gagagaacgc tggaatgcat aaaggaggca
     1921 gacaccacag tcatgcagat gcagatcaag agacagagag agttctggca cttactgaag
     1981 attgcctgtg cacaaaacac cacacggagc tcagtgagtc agagcggaga aatgccctcc
     2041 tcgctgtcta cttggaacca gactcaagcc cagtgttcct cccgtctccc catgtccttg
     2101 caagtgccac acgagggtga ttcagttaat catttactgg aggagaatca gcgttacttg
     2161 actcagctca ccagtttact gcaagaaacc accgaggaga aatcagagag cataatggct
     2221 caggactgga gctggacaaa atacgaatcc ctggttgcta aatcagcgcg tttgtatacg
     2281 gatccaccgg tcgccaccat ggtgagcaag ggcgccgagc tgttcaccgg catcgtgccc
     2341 atcctgatcg agctgaatgg cgatgtgaat ggccacaagt tcagcgtgag cggcgagggc
     2401 gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg
     2461 cctgtgccct ggcccaccct ggtgaccacc ctgagctacg gcgtgcagtg cttctcacgc
     2521 taccccgatc acatgaagca gcacgacttc ttcaagagcg ccatgcctga gggctacatc
     2581 caggagcgca ccatcttctt cgaggatgac ggcaactaca agtcgcgcgc cgaggtgaag
     2641 ttcgagggcg ataccctggt gaatcgcatc gagctgaccg gcaccgattt caaggaggat
     2701 ggcaacatcc tgggcaataa gatggagtac aactacaacg cccacaatgt gtacatcatg
     2761 accgacaagg ccaagaatgg catcaaggtg aacttcaaga tccgccacaa catcgaggat
     2821 ggcagcgtgc agctggccga ccactaccag cagaataccc ccatcggcga tggccctgtg
     2881 ctgctgcccg ataaccacta cctgtccacc cagagcgccc tgtccaagga ccccaacgag
     2941 aagcgcgatc acatgatcta cttcggcttc gtgaccgccg ccgccatcac ccacggcatg
     3001 gatgagctgt acaagtgagc ggccgccacc gcggtggagc tcgaattaat tcatcgatga
     3061 tgatccagac atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg
     3121 aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag
     3181 ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga
     3241 ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa tgtggtatgg ctgattatga
     3301 tcctctagat cagatctgtc ccgatctttc tcttctgtgc tgtcaattta gagtagtttt
     3361 tgaggtaatt tgggtgcatt ctctgtaaaa acaacaaata aatatttata ttgtatgcaa
     3421 taaatgtaac aattacacat acactctctg attacagcat taaagggtta gttcacccaa
     3481 aaatgaaaag atgtcattaa tgactcaccc tcatgtcgtt ccaagcccgt aacacctccg
     3541 ttcatcttcg gaacacagtt taagatattt tagatttagt ccgagagctt tctgtgcctc
     3601 cattgagaat gtatgtacgg tatactgtcc atgtccagaa aggtaataaa aacatcaaag
     3661 tagtccatgt gacatcagtg ggttagttag aattttttga agcatcgaat acattttggt
     3721 ccaaaaataa caaaacctac gactttattc ggcattgtat tctcttccgg gtctgttgtc
     3781 aatccgcgtt cacgacttcg cagtgacgct acaatgctga ataaagtcgt aggttttgtt
     3841 atttttggac caaaatgtat tttcgatgct tcaaataatt ctacctaacc cactgatgtc
     3901 acatggacta ctttgatgtt tttattacct ttctggacat ggacagtata ccgtacatac
     3961 attttcagtg gagggacaga aagctctcgg actaaatcta aaatatctta aactgtgttc
     4021 cgaagatgaa cggaggtgtt acgggcttgg aacgacatga gggtgagtca ttaatgacat
     4081 tattttcatt tttgggtgaa ctaacccttt aataaaactg acatttgcag ccaattgcac
     4141 actttataat gttaaatact tgttgatttt agaaatacag tagcatttag tgacaatcaa
     4201 acattaattt tatttctgat tatattgaca aaaacacagg actgtaaggc tgacatgaca
     4261 gggtgaattg atttcattac attagttttg atggtaacac ttttacaata aggttcgtta
     4321 gttaactaca ttagttaaca tgaactaata atgaactgca cgtatacatg ttaatttcaa
     4381 catttactaa tactttatat cttgttaaca ttagttaatg cactgtgaac taacatgaac
     4441 aaacaatgaa catctgtatt tctattaact aacgttaaca aagattaaca gatacagtaa
     4501 caaatgtatt gctcatggtt atttaatgtt agttaataca tgaactaatc aacctcattg
     4561 taaagtgtta ccgttttgac aatacaacat gtttggaaaa aaatttctcg cttggttttc
     4621 ttgtgttttg catgaattca aacacaatga gagcacggaa tgataatgat tattcaaagc
     4681 tgcaataggc gccaaatttc ccgtggacct aacgttacca attacaaatg cattttagca
     4741 ttttattacg tgagccttat attgtcaatc gcatgcatta gtgaaaatag gcctactaca
     4801 catataaaag gcgcgccatc aacgtcaaaa catgcacacc caaaaaaaaa aaaaaaaaaa
     4861 aaaaaaanct atcagtaaaa caaaatactt aatgtactta cctcaatatg cttccttagg
     4921 tttgatggcg aacttttgaa ggccgatatt tctttattaa gcgggagaca gaggacacat
     4981 ttcatcttga atgaatcttt atttacacca cttaaagaaa agaattcgcg aagatacggc
     5041 cacgggtgct cttgatcctg tggctgattt tggactgtgc tgctcgcagc tgctgatgaa
     5101 tcacatactt cctccatttt cttccactga ttgactgtta taatttccct aatttccagg
     5161 tcaaggtgct gtgcattgtg gtaatagatg tgacatgacg tcacttccaa aggaccaatg
     5221 aacatgtctg accaatttca tataatgtga aaacgatttt cataggcaga ataaataaca
     5281 tttaaattaa actgggcatc agcgcaattc aattggtttg gtaatagcaa gggaaaatag
     5341 aatgaagtga tctccaaaaa ataagtactt tttgactgta aataaaattg taaggagtaa
     5401 aaagtacttt tttttctaaa aaaatgtaat taagtaaaag taaaagtatt gatttttaat
     5461 tgtactcaag taaagtaaaa atccccaaaa ataatactta agtacagtaa tcaagtaaaa
     5521 ttactcaagt actttacacc tctggttctt gaccccctac cttcagcaag cccagcagat
     5581 ccactagttc tagagcggcc ggccgccacc gcggtggagc tccagctttt gttcccttta
     5641 gtgagggtta attgcgcgct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg
     5701 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
     5761 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     5821 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     5881 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     5941 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     6001 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     6061 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     6121 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     6181 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     6241 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     6301 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     6361 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     6421 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     6481 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     6541 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     6601 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     6661 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     6721 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     6781 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     6841 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     6901 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     6961 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     7021 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     7081 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     7141 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     7201 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     7261 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     7321 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     7381 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     7441 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     7501 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     7561 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     7621 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     7681 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     7741 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     7801 cacatttccc cgaaaagtgc cacctaaatt gtaagcgtta atattttgtt aaaattcgcg
     7861 ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct
     7921 tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt
     7981 ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat
     8041 ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca
     8101 ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac
     8161 gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta
     8221 gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg
     8281 tcccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg
     8341 ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca
     8401 gggttttccc agtcacgacg ttgtaaaacg acggccagtg agcgcgcgta atacgactca
     8461 ctatagggcg aattgggtac caaatagtag gaattaccca cctgtacaag tgctgaaaac
     8521 ttggatgaat aagcccgttt gccattttct actgctattt taaatctttt ctgtatttgt
     8581 ctgcatttgt ctttaccctt gaataaatgt tttagttgtt ttttttcaat tatgactgtg
     8641 tttaacagac attattacaa taatatgtaa tagtagtaca cactattatt atgtaatagt
     8701 acttgttgac tgtatttgag aactggaccg agtgagtgtt acgtcaccca ttcaaaatga
     8761 cttacttctg gctccaacaa aatgaagtta attcagttgc catttttcac tgtatggaca
     8821 tcgccgtgtt ggagctagac gttgccaata agcaagaaag agccgagatg cgtcgagtct
     8881 gagtcaccgt tcctatggca acccctctaa ccaatcagaa gtaagcttgt tggaagtcca
     8941 cagcctacca cttgaaagcg ggctgcacaa aatctgtcaa acgttttgaa cgttggatgt
     9001 gagagcacat acttttatta aggcatctgg ttggtcagtt tataatatca acaacttggg
     9061 ctacagaaaa gaaaagttat tacagaaatt atgattaaca agtacatgtt aaataaagat
     9121 tttaatatga atgccaccac tggagcattc atgccatttg gagcttcttc ctgtttggat
     9181 cactagaagg aggaggtcac tcattacagt tctcatatac agtcgttggt tggttggttg
     9241 gttggtagat tgattgattg attgattgat tgattgattg attgattgat tgattgattg
     9301 attgattgat tgattgattg attggtagtc aaaataagaa ataatttcca cagattcatt
     9361 acagaaatga ttaaatgcat acataaaaaa ctgggggggg ggggggatac aacaacacac
     9421 ttaagtcaca tttgcctacg taaagaaaag taaagaaaat caatagctat attttacatc
     9481 tccttttttt gctgtctttt aattagcctt gttttgctgt tatcttgatt gaaactgtaa
     9541 cttcttcacc tgcttctttt ctttgtaggt tttgccagcc cagaaacagg catggctgtg
     9601 caaggccaga gcaccatgca caatgcgctg catgtcttca tgaacggctc aatgtcctca
     9661 gtccagggct cagccaacga ccccatcttc ctccttcacc atgctttcat tgacaggtaa
     9721 caaacacgtc atgacattag actgcacagt ttttgacaaa gttcatacaa tctgttgttt
     9781 atagctgcta caattagtga agtttgtgaa tgtacttgga tgagcagcga aagatcaatt
     9841 gagatcaatt gttagagttt ggttgccctg cagagcaaag aacaaaaaat aatctggtgg
     9901 ctttactgcg tgaggttatt attggtggaa tagaaacaca aaacataatt gcatttattt
     9961 gtttaatttt ttatcttatc ttaactttca tcttgcatat ttgtttctta catcatttct
    10021 agcatctttg agcgctggct aagaactcat cagcctcccc ggtccatcta cccacgtacc
    10081 aatgcaccaa ttggccacaa tgacggctac tacatggtgc cattccttcc tctttatagg
    10141 aatggagact acctcctgtc caacaatgct cttggatacg agtacgccta cctgttggac
    10201 ccaggtcatt gcacaacacc agaaatgccc tctgatctgc aaaagacgtg aatatctgtt
    10261 cagacaccca tatccactct gttccacaca ggtcagaggt ttgtccagga gttcttgaca
    10321 gaggtgtaaa aagtactcaa aaattttact caagtgaaag tacaagtact tagggaaaat
    10381 tttactcaat taaaagtaaa agtatctggc tagaatctta cttgagtaaa agtaaaaaag
    10441 tactccatta aaattgtact tgagtattaa ggaagtaaaa gtaaaagcaa gaaagaaaac
    10501 tagagattct tgtttaagct tttaatctca aaaaacatta aatgaaatgc atacaaggtt
    10561 ttatcctgct ttagaactgt ttgtatttaa ttatcaaact ataagacaga caatctaatg
    10621 ccagtacacg ctactcaaag ttgtaaaacc tcagatttaa cttcagtaga agctgattct
    10681 caaaattgtt agtgtcaagc ctagctcttt tggggctgaa aagcaatcct gcagtgctga
    10741 aaagcctctc acaggcagcc gatgcgggaa gaggtgtatt agtcttgata gagaggctgc
    10801 aaatagcagg aaacgtgagc agagactccc tggtgtctga aacacaggcc agatgggccc
    10861 tcgagagaaa ccaggctcag tcgggcacga ccattactcc acttgaccaa acgtctgcat
    10921 atttataaat aataatttgc gtattaatat gcagataaag acaattaaaa gaagcaacag
    10981 tagcattaat cattcaaata acagtataca atcaaacaac attttgtata tatatttaaa
    11041 aaaacaatac aaggagtaaa gacaaaggca atatcattta gtttttttaa tattaacaaa
    11101 ccacaggctg tgtgaaccat cagcaatttc aaaccagagg gatctgcttt tttgggaacg
    11161 atgttgcaaa tttaatctct agatttttta aatcatggat agcctcccta aagcaaaagc
    11221 tttttagggg ctttacttct cctgattgca gcctgtggta taatttgtga ttgttaggtg
    11281 ggtcaggttt tggggtccag ccctttgtgc actttgacta ttgtcacctt aggctgtccc
    11341 ttaatgctgc cacctctgac cccggtggga cacataaata acttctccac ccgagttagc
    11401 atttcaagcc agtgcaataa agaaattaaa aataattgat ttattaacaa ctctgtttta
    11461 tctttaaaaa ataaaatgtt ttatatctaa tgttcactca ggtacctgat attgtttgct
    11521 gtgcaataat gtaggttagt cattatattg acaaagaaaa ttaaatatga tgttgtttat
    11581 ttttaatttt acattataga ctagattaga ttttcatttg gaatttaatt aggagtgcct
    11641 ggagtgcatt gagaacgtta aaaaaaaaaa atctgcttac tcttatagct ttggaaacta
    11701 ttttcagatt attactccac tatagaagcg attccttttt ttgaataatt aaatatatat
    11761 atacagtgct cagcataaat gatcatcaca gatctctatt ttaaagtatt atttttccat
    11821 aggatgcttt acaataatat atgtgtgtgt atacattaga attgtcagtt acaaaatcaa
    11881 aactgcaaca aaataactaa acttaagatc taatgagtac acccaaatta aaccaaacat
    11941 tagtctatgc aaaaattaat atgttaagga aaaatattaa atacaatttt aatattcagt
    12001 cattcatttt cttttttgct tagtcccttt attaatctgg ggtcgccaca gtggaattaa
    12061 ctgccaattt atccagcata tattttatgc agcggatgct cttccagctg ctacccatca
    12121 ctgggaatca cccatacact ctcattcaca cacatacact acggacaatt tagcctaccc
    12181 aattcaccta tagcgtatgt ctttggactt gtgggggaaa ccggaacaca cggaggaaac
    12241 ccacgcgaac acagggagat catgcaaact cagaaacgcc aactgatcca gccaaggctc
    12301 gaaccagtga ccttcttgct gtgaggcaat agggctaccc attgtgtcac tgcaccgtcc
    12361 acaattttaa taaacatgaa aaatcccgag aaacataaac aatattaaat gtagttgaaa
    12421 ttttgttgtt tgaaattgta tttattaaat aaaaaaaatg tatgtattat tattatttat
    12481 ttatttataa aagatgttag gtggccaaac tctttttttt aatggatata gttgtttaat
    12541 aaaacaattt tgtttaaata aaacaaaata tattgaatga gccatgttgc tgttgttgtt
    12601 ttttacaggc ttgatgtaca atatgatctt tgtgtagttt tatagtaata aatcattcat
    12661 gtcttgtgta ttgtacgtca tattatctta aaagtacgct attaattaaa cttgtttata
    12721 tggtcatctt agacaggact ctcattataa aaggattttt gttaatttta ttagtacctt
    12781 cctggaaaaa taaaagataa ataaataaaa atgaataaaa gtcttgggct tgttttctgt
    12841 acgagtaata aaaaagagta agcaaatatt ctgcatgaca atattgttca aagtttaagg
    12901 gctgtatttt ttataattgt tggttattaa catgttttat acaaattact ttcaacatgg
    12961 ctgaaataat aattgttatc actatttgaa acagtcctag aatagatttt actatattta
    13021 catttattcc tctgatgaca aagttgaaat tcctctgatg acaaagcaaa actctcaact
    13081 ctagttttat tttattgtaa taaccatgtc aagggataat gacaatgttt tccattatat
    13141 tttcaatata gaataaaatt ataatttatt aatcatttag tagggcagca tggtggcaca
    13201 atgggtagca caatcacctc acaccaagaa ggttgctagt tcgagccccg gctgggtcag
    13261 tttgcatatc tgtgtggaga atgttctctc tgtgttgcca tgggtttcct ctgggtgctc
    13321 tagtttccac cacaagtcta aaaacaagtg gcatcggtga attgtgtagg ctaaattgtc
    13381 tgtagtgttt gtgtgtgatt gagtgtttat ggatgtttcc cagtgatggg ttgcagttgg
    13441 aagggcattc tctgcgtaaa actataagtt ggcggttcat tccactgtgg tgaccccaga
    13501 ttaataaagg gattaagccg aaaagaaaat gaatcattaa atgatcattt agttgattgg
    13561 agagcattgc ataatgagaa tcaaataatg tatagttttt tgtaaaatga ttgtgtatta
    13621 gaggcacatt gtacgtagtg tggaatgttt aggttgtctt tctgattgtt ttcattttgc
    13681 agaagttcat ttgcagtcag tgtagtcaaa gcacctcagc tcaatttata gcagcagcct
    13741 gctgtgattt aagtgccaca ggagtgaagg ctttaacccc tgttgtagat tgcaaccaat
    13801 gtttttctaa ggtaattaga agcttgttta gctaacgcta cccaccccac cccccacaaa
    13861 aaagttgctc tgaatagcag catctacccc caggagggtg caagcttctg ttttgaagaa
    13921 atttgctcat ggtgttacat aggtgtttaa aaatgtatgt tttttaatag catatatttt
    13981 aaaagtgact cattaatgac atcatctgcc atctgccttt ctcagaactg acaaaggacc
    14041 cttttaagga cccctataca ccacattttt atctatctaa tcacaagtgg gtgatattag
    14101 agggatgatt cacctaaaaa tgaattttcc gcattttaat ttggggttcc aaaccagttt
    14161 gaattctttt gttctgttga acacaaaata agatgtttta agaatgttgg aaatgttgga
    14221 gacttttata gtaggaaaaa catatactaa tatttgcatt catgtttgaa atgtttaaaa
    14281 catttttcaa aatatcttat ttttcaactg ttttggaaca agtcaaaagt gagtaaatga
    14341 tgactgataa acaatttctt taaatacaga tgaaaatagc gtctaacaca ttttgaacta
    14401 gttcactttc aaccacagca agaggtagtc gaaaacacat ttgatcacat tttgttgtgg
    14461 gaccgcttgt tgttcagtgt gttttcattg ttgagttaca aaccggtaag tggcattaaa
    14521 acatgttttg gcatcactga tgatcataaa taaattggtt aaaaaattaa aaattagatg
    14581 cttacagtac agtttgttta aaaacttgca ctttttgtag tgtgaaatga cattagtacc
    14641 tgcagtgggc tttctttgtt ctgtatcttc tgatgaatct aacataaaga catttaaata
    14701 ttcacaatgg agtgggttgg ttctcatcta tctgaaacaa cattagtgtg ttcaaaacaa
    14761 atctacacca gtgatctctg atagatacaa gctgtcaacc gtagtcctat tgtttctcat
    14821 gcgactgtca cagggccatg ctagaggctc tttgggtggt gagtagagac tcattcttat
    14881 gcaaacaggc tatggatgct acaactgaag agaatttagt gctctaaggt catcgcagtt
    14941 ctataaagca gattccctcc aaaaagctag agaaaattat gtgtctggag gcaccatcta
    15001 tcagtgcagt cattctttta ttactttttt tttttgtcca tgcaattttt taatcgcatt
    15061 ttgaagtatt taaaccatta agagtgtcaa attatcatgt tattatttca actcataata
    15121 tctcctttta aagatgattc atttgattgt agttataata attgttttat taatgcaaaa
    15181 attgcttaaa tgtttggcct ctgagatttt cagttgactc aatatttttg gagcctgttt
    15241 catgacttta tttaaaatat aatctgcact cgcaaatatt acaattatat catattttga
    15301 aaatcttaga tgtacatttg ttttgaaaat ggtttaaagg aaatttaggt caaaatgtgt
    15361 gtttttttta aataataatt cagtcttttt tttgatataa tagggaaaat gacagcaatt
    15421 aaaaggctgt tttggaaaga catctaggtc tggtgcagac ttcgtaactt cttagagtta
    15481 aatcttgaaa acggagccag aagacgggca acaccttcct aaaagcaggg tggggtgttg
    15541 gacaggtagc ttcttggaac ctcccctctt tgtcgctgtg gtatatatag aaatcccatt
    15601 gaattcctat agagacctta agcccgtacc atttacctct gaaggatcct aat
//
