LOCUS       sequence-202867-        8683 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  tdTomato expression in differentiated keratinocytes of the periderm 
            layer and during early larval stages also in the basal layer of the 
            epidermis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 8683)
  TITLE     Rieger lab plasmids
REFERENCE   2  (bases 1 to 8683)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pDest-Tol2 (Chien)-Krt4:Gal4VP16:tdTomato:5xUA...
FEATURES             Location/Qualifiers
     source          1..8683
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             241..681
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     polyA_signal    954..1088
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(1213..1914)
                     /codon_start=1
                     /product="dimeric variant of DsRed fluorescent protein 
                     (Shaner et al., 2004)"
                     /label=dTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLYGMDELYK"
     primer_bind     complement(1296..1319)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(1494..1513)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     1763..1781
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      1911..1920
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    2387..2521
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    2614..2748
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    join(3187..3374,3375..3386)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(3420..3436)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3420..3436)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(3473..3493)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(3473..3491)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3512..3528)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3512..3528)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3525..3547)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3536..3552
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3560..3566,3567..3584,3585..3590))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3605..3626
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3743..3760)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3914..4502)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3994..4013)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4673..5464,5465..5533))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5296..5315
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5534..5638)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(5801..5822)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    join(6171..6182,6183..6345)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
ORIGIN
        1 atgcctgtcc tcaagtctca agcctctcct tgcattttct cattccacct ggggcaaagc
       61 cccaggctgg gtgtgacacc atttatctta ccactttctc tctgtacctg tctaacaggt
      121 agggtgtgtg tgagagtgcg tatgtgtgca agtgtgtgtg tgagagcagt cagctccacc
      181 ccctcaagag tgtgtataaa attggtcagc cagctgctga gagacacgca gaggcctagg
      241 atgaagctac tgtcttctat cgaacaagca tgcgatattt gccgacttaa aaagctcaag
      301 tgctccaaag aaaaaccgaa gtgcgccaag tgtctgaaga acaactggga gtgtcgctac
      361 tctcccaaaa ccaaaaggtc tccgctgact agggcacatc tgacagaagt ggaatcaagg
      421 ctagaaagac tggaacagct atttctactg atttttcctc gagaagacct tgacatgatt
      481 ttgaaaatgg attctttaca ggatataaaa gcattgttaa caggattatt tgtacaagat
      541 aatgtgaata aagatgccgt cacagataga ttggcttcag tggagactga tatgcctcta
      601 acattgagac agcatagaat aagtgcgaca tcatcatcgg aagagagtag taacaaaggt
      661 caaagacagt tgactgtatc gtcgaggtcg accccgggaa ttcagatctc tcgagccgcc
      721 cccccgaccg atgtcagcct gggggacgag ctccacttag acggcgagga cgtggcgatg
      781 gcgcatgccg acgcgctaga cgatttcgat ctggacatgt tgggggacgg ggattccccg
      841 ggtccgggat ttacccccca cgactccgcc ccctacggcg ctctggatat gtagcggatc
      901 tactagtgcg gcccctctcg agcctctaga actatagtga gtcgtattac gtagatccag
      961 acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat
     1021 gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata
     1081 aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg
     1141 aggtttttta aagcaagtaa aacctctaca aatgtggtat ggctgattat gatctagagt
     1201 cgcggccgcc tacttgtaca gctcgtccat gccgtacagg aacaggtggt ggcggccctc
     1261 ggagcgctcg tactgttcca cgatggtgta gtcctcgttg tgggaggtga tgtccagctt
     1321 ggtgtccacg tagtagtagc cgggcagttg cacgggcttc ttggccatgt agatggtctt
     1381 gaactccacc aggtagtggc cgccgtcctt cagcttcagg gcctggtgga tctcgccctt
     1441 cagcacgccg tcgcgggggt acaggcgctc ggtggaggcc tcccagccca tggtcttctt
     1501 ctgcattacg gggccgtcgg gggggaagtt ggtgccgcgc atcttcacct tgtagatcag
     1561 cgtgccgtcc tgcagggagg agtcctgggt cacggtcacc agaccgccgt cctcgaagtt
     1621 catcacgcgc tcccacttga agccctcggg gaaggacagc ttcttgtaat cggggatgtc
     1681 ggcggggtgc ttcacgtacg ccttggagcc gtacatgaac tggggggaca ggatgtccca
     1741 ggcgaagggc agggggccgc ccttggtcac cttcagcttg gcggtctggg tgccctcgta
     1801 ggggcggccc tcgccctcgc cctcgatctc gaactcgtgg ccgttcatgg agccctccat
     1861 gcgcaccttg aagcgcatga actctttgat gacctcctcg cccttgctca ccatggtggc
     1921 gaccggtgga tcccgtctag agggtatata atggatccca tcgcgtctca gcctcacttt
     1981 gagctcctcc acacgaattc tttggcccgg agtactgtcc tccgggctgg cggagtactg
     2041 tcctccggca aggtcggagt actgtcctcc gacactagag gtcggagtac tgtcctccga
     2101 cgcaaggcgg agtactgtcc tccgtctaga gggtatataa tggatcccat cgcgtctcag
     2161 cctcactttg agctcctcca cacgaattcc ctcgacctcg aagaccttaa gatggattgg
     2221 ggcacgctgc agacgatcct ggggggtgtg aacaaacact ccaccagcat tggaaagatc
     2281 tggctcaccg tcctcttcat ttttcgcatt atgatcctcg ttgtggctgc aaaggaggtg
     2341 tgggaagatg agcaggccga cttatccttt cttgtacaaa gtgggggatc cagacatgat
     2401 aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat
     2461 ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt
     2521 taacaacaac aattgcattc attttatgtt tcaggttcag ggggaggtgt gggaggtttt
     2581 ttccaacttt attatacagg taccatcgat gatgatccag acatgataag atacattgat
     2641 gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt
     2701 gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat
     2761 tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggtttttta aagcaagtaa
     2821 aacctctaca aatgtggtat ggctgattat gatcctctag atcagatctg cgaagatacg
     2881 gccacgggtg ctcttgatcc tgtggctgat tttggactgt gctgctcgca gctgctgatg
     2941 aatcacatac ttcctccatt ttcttccact gattgactgt tataatttcc ctaatttcca
     3001 ggtcaaggtg ctgtgcattg tggtaataga tgtgacatga cgtcacttcc aaaggaccaa
     3061 tgaacatgtc tgaccaattt catataatgt gaaaacgatt ttcataggca gaataaataa
     3121 catttaaatt aaactgggca tcagcgcaat tcaattggtt tggtaatagc aagggaaaat
     3181 agaatgaagt gatctccaaa aaataagtac tttttgactg taaataaaat tgtaaggagt
     3241 aaaaagtact tttttttcta aaaaaatgta attaagtaaa agtaaaagta ttgattttta
     3301 attgtactca agtaaagtaa aaatccccaa aaataatact taagtacagt aatcaagtaa
     3361 aattactcaa gtactttaca cctctggttc ttgaccccct accttcagca agcccagcag
     3421 atccactagt tctagagcgg ccgccaccgc ggtggagctc cagcttttgt tccctttagt
     3481 gagggttaat tgcgcgcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     3541 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     3601 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     3661 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     3721 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     3781 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     3841 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     3901 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     3961 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     4021 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     4081 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     4141 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     4201 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     4261 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     4321 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     4381 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     4441 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     4501 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     4561 aaggcatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     4621 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     4681 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     4741 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     4801 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     4861 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     4921 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     4981 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     5041 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     5101 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     5161 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     5221 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     5281 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     5341 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     5401 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     5461 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     5521 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     5581 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     5641 catttccccg aaaagtgcca cctaaattgt aagcgttaat attttgttaa aattcgcgtt
     5701 aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta
     5761 taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc
     5821 actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg
     5881 cccacgctgg ctaagaactc atcagcctcc ccggtccatc tacccacgta ccaatgcacc
     5941 aattggccac aatgacggct actacatggt gccattcctt cctctttata ggaatggaga
     6001 ctacctcctg tccaacaatg ctcttggata cgagtacgcc tacctgttgg acccaggtca
     6061 ttgcacaaca ccagaaatgc cctctgatct gcaaaagacg tgaatatctg ttcagacacc
     6121 catatccact ctgttccaca caggtcagag gtttgtccag gagttcttga cagaggtgta
     6181 aaaagtactc aaaaatttta ctcaagtgaa agtacaagta cttagggaaa attttactca
     6241 attaaaagta aaagtatctg gctagaatct tacttgagta aaagtaaaaa agtactccat
     6301 taaaattgta cttgagtatt aaggaagtaa aagtaaaagc aagaaagaaa actagagatt
     6361 cttgtttaag cttttaatct caaaaaacat taaatgaaat gcatacaagg ttttatcctg
     6421 ctttagaact gtttgtattt aattatcaaa ctataagaca gacaatctaa tgccagtaca
     6481 cgctactcaa agttgtaaaa cctcagattt aacttcagta gaagctgatt ctcaaaattg
     6541 ttagtgtcaa gcctagctct tttggggctg aaaagcaatc ctgcagtgct gaaaagcctc
     6601 tcacaggcag ccgatgcggg aagaggtgta ttagtcttga tagagaggct gcaaatagca
     6661 ggaaacgtga gcagagactc cctggtgtct gaaacacagg ccagatgggc cctcgagcag
     6721 gtaaacttta agtacataaa acatgcattt actgcataat aacaaataaa taatgcccca
     6781 aaactcttgt gcaccttcct tctgactggc caaacccctg gccaagccct gtctgattag
     6841 tgtaatagtt tgctacagta aagcttctcc acaatgtccc ggagatccag cccggacatg
     6901 cattttcttg cctttccttc ccttcaactt ctgacgtcct tttaagagct tgtgcatgaa
     6961 agcaaattta gagcttatta ctcatctcaa acacccatac aaagtgatga ttgctgtacc
     7021 ataatctcac acctttcaca cctggtttat actatgatag ttgtagacga ttgcgtaatg
     7081 ctattaaatg cccatcagtg ctggctgtga cacccaactg ctgccatttc gtgttgactt
     7141 gcacgagaaa tgagaaattg tctgactatg cagggtgtac tatgcgtggg aacttatcag
     7201 tggtcattaa atactatggt ttaaagttag agcaactttt actttccctt gagtacattt
     7261 ttagtgcagt atttgtactt ttatttcact actttccttc aacctgcagt cactacttta
     7321 ttttttcttg tctatgtgga ttagacaaat cagtcctgtg attcctgtcc aatcaaatcg
     7381 cacatagaag gtaaatcaca tcataatgaa cttcctcaag acatgggcca tttataattg
     7441 cagcaaactg tttgccagaa ttaaaagaag atgtcaaaaa tctttacccg cattaaccca
     7501 gagactgctt agatgcatgt cactgatgag aagatgacgg atgttttctg tatgatgacc
     7561 gaaataacct taaacgcaca caagacggca caagacgtca acatggctat aggtttacgt
     7621 tgtaccccat tgcaatgggg acgttgtatt ttgtttagaa atgaaaatta ggttgacgtc
     7681 agagctcaac gtcaggtcga tgtcaaggtt caacatccaa tctaaaatca tatatcaatg
     7741 tctaatgatg tacagcttga tgttgtgtgg atgttacccc tatgatgtct atcagaagtt
     7801 ggattatggt tgccatacct aatgaataaa tgtcattatt ttacacttgt ttaagatgtt
     7861 ggttcgacat tggacttatg tcgctttcca acacaaccta aatccaccaa atattaactt
     7921 cctatgacat cgttattgga catcaaaata acaatatcct tagatgctgg ctagactttg
     7981 aatttaggtc accacaacct atatttaacc taatattaac atcttatgat gttgtgtgcc
     8041 tgctgggcaa taactaaatg cactacagaa tgttacgttt acacacatgt aaattacatg
     8101 taaatgcatt agctttttaa ggcataatac tcactactta ctactcttga gtacttttaa
     8161 aaaagctact ttttactcat actttgagta acagttacaa cagatacttt tactcgcact
     8221 ggatttttag gcatgtaatg gtatttttac tatgattttt cagtactctt tccactactg
     8281 cagccctccc catacataat catatgttta cacatatggt ggagtttaga gccataaact
     8341 acattagctt tgttagccgc tagcattact gtgcagaatt gtgtgtgtgc acattttcca
     8401 atatcaatac agaaggaaac tctgttccct gttcccttgt aaatctcaac aatgcaactg
     8461 ttcagctcag ggggaaaaat gccctgccag atccaaacgc tggcaaaagt ggatggaaaa
     8521 aagcctttca ttaatgtaag agttgctgcg cgccccaccc agataaaaag agcagaggtt
     8581 aacatgctct ctacggctgt ccagccaacc agatactgag gcagaaacac acccgcttgc
     8641 agatggtgag agctacactg tctttttcag agtttctact gga
//
