LOCUS       sequence-202874-        1084 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Cas9 upon induction with doxicycline..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1084)
  AUTHORS   Castano J, Bueno C, Jimenez-Delgado S, Roca-Ho H, Fraga MF, 
            Fernandez AF, Nakanishi M, Torres-Ruiz R, Rodriguez-Perales S, 
            Menendez P
  TITLE     Generation and characterization of a human iPSC cell line expressing
            inducible Cas9 in the ""safe harbor"" AAVS1 locus.
  JOURNAL   Stem Cell Res. 2017 May;21:137-140. doi: 10.1016/j.scr.2017.04.011. 
            Epub 2017 Apr 22.
  PUBMED    28677529
REFERENCE   2  (bases 1 to 1084)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cell 
            Res."; date: "2017-05"; volume: "21:137-140. doi"; pages: " 
            10.1016/j.scr.2017.04.011. Epub 2017 Apr 22"
COMMENT     Sequence Label: pAAVS1-TRE-Cas9- puro-polyA-CAG-rtTA
FEATURES             Location/Qualifiers
     source          1..1084
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        29..47
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    264..765
                     /label=HA-L
                     /note="left homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     misc_feature    893..919
                     /label=SA
                     /note="splice acceptor site"
ORIGIN
        1 caaaaaggac atgattacgc cagctcagaa ttaaccctca ctaaagggac tagtcctgca
       61 ggtttaaacg aattcgccct ttgctttctc tgaccagcat tctctcccct gggcctgtgc
      121 cgctttctgt ctgcagcttg tggcctgggt cacctctacg gctggcccag atccttccct
      181 gccgcctcct tcaggttccg tcttcctcca ctccctcttc cccttgctct ctgctgtgtt
      241 gctgcccaag gatgctcttt ccggagcact tccttctcgg cgctgcacca cgtgatgtcc
      301 tctgagcgga tcctccccgt gtctgggtcc tctccgggca tctctcctcc ctcacccaac
      361 cccatgccgt cttcactcgc tgggttccct tttccttctc cttctggggc ctgtgccatc
      421 tctcgtttct taggatggcc ttctccgacg gatgtctccc ttgcgtcccg cctccccttc
      481 ttgtaggcct gcatcatcac cgtttttctg gacaacccca aagtaccccg tctccctggc
      541 tttagccacc tctccatcct cttgctttct ttgcctggac accccgttct cctgtggatt
      601 cgggtcacct ctcactcctt tcatttgggc agctccccta ccccccttac ctctctagtc
      661 tgtgctagct cttccagccc cctgtcatgg catcttccag gggtccgaga gctcagctag
      721 tcttcttcct ccaacccggg cccctatgtc cacttcagga cagcatgttt gctgcctcca
      781 gggatcctgt gtccccgagc tgggaccacc ttatattccc agggccggtt aatgtggctc
      841 tggttctggg tacttttatc tgtcccctcc accccacagt gggggcaagc ttctgacctc
      901 ttcttcttcc tcccacaggg gcctcgaaga gatctggcag cggagaaggg caagaggagt
      961 cttctaacat gcggtgacgt ggaaggagaa tccgggccct agctcaagat gaccggagta
     1021 caagcccacg gtgcgccctt cactaccccg cgaacgacgt ctccagggga cgatagagaa
     1081 acct
//
