LOCUS       sequence-202877-        1159 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Cas9 upon induction with doxicycline..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1159)
  AUTHORS   Castano J, Bueno C, Jimenez-Delgado S, Roca-Ho H, Fraga MF, 
            Fernandez AF, Nakanishi M, Torres-Ruiz R, Rodriguez-Perales S, 
            Menendez P
  TITLE     Generation and characterization of a human iPSC cell line expressing
            inducible Cas9 in the ""safe harbor"" AAVS1 locus.
  JOURNAL   Stem Cell Res. 2017 May;21:137-140. doi: 10.1016/j.scr.2017.04.011. 
            Epub 2017 Apr 22.
  PUBMED    28677529
REFERENCE   2  (bases 1 to 1159)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cell 
            Res."; date: "2017-05"; volume: "21:137-140. doi"; pages: " 
            10.1016/j.scr.2017.04.011. Epub 2017 Apr 22"
COMMENT     Sequence Label: pAAVS1-TRE-Cas9- puro-polyA-CAG-rtTA
FEATURES             Location/Qualifiers
     source          1..1159
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    40..58
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    76..94
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    111..129
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    147..165
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    183..201
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    218..236
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    254..272
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 gnnnnnngct ccaccgtaca cgcctacctc gacatacgtt ctctatcact gatagggagt
       61 aaactcgaca tacgttctct atcactgata gggataaact cgacatacgt tctctatcac
      121 tgatagggag taaactcgac atacgttctc tatcactgat agggagtaaa ctcgacatac
      181 gttctctatc actgataggg agtaaactcg acatcgttct ctatcactga tagggagtaa
      241 actcgacata cgttctctat cactgatagg gagtaaactc gacagtacta agctttacta
      301 gggacaggat tggtgacaga aaagccccat ccttaggcct cctccttcct agtctcctga
      361 tattgggtct aacccccacc tcctgttagg cagattcctt atctggtgac acacccccat
      421 ttcctggagc catctctctc cttgccagaa cctctaaggt ttgcttacga tggagccaga
      481 gaggatcctg ggagggagag cttggcaggg ggtgggaggg aaggggggga tgcgtgacct
      541 gcccggttct cagtggccac cctgcgctac cctctcccag aacctgagct gctctgacgc
      601 ggctgtctgg tgcgtttcac tgatcctggt gctgcagctt ccttacactt cccaagagga
      661 gaagcagttt ggaaaaacaa aatcagaata agttggtcct gagttctaac tttggctctt
      721 cacctttcta gtccccaatt tatattgttc ctccgtgcgt cagttttacc tgtgagataa
      781 ggccagtagc cagccccgtc ctggcagggc tgtggtgagg aggggggtgt ccgtgtggaa
      841 aactcccttt gtgagaatgg tgcgtcctag gtgttcacca ggtcgtgggc cgcctctact
      901 ccctttctct ttctccatcc ttcttttcct taaagagtcc ccagggctat ctgggtacat
      961 attccctccc acccagaaca tggatcccgc cttccctaaa agcactgact ctaggacttc
     1021 tgggtttnna ngtcctttga caagncccaa atnnnaggct cctcaaggct ttccctgttc
     1081 ccactgtact tcnnnacgac catcgttaga tatcccatga catacgtcct agncngcatn
     1141 nccctgaatc agagnngtc
//
