LOCUS       sequence-202878-        1186 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Cas9 upon induction with doxicycline..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1186)
  AUTHORS   Castano J, Bueno C, Jimenez-Delgado S, Roca-Ho H, Fraga MF, 
            Fernandez AF, Nakanishi M, Torres-Ruiz R, Rodriguez-Perales S, 
            Menendez P
  TITLE     Generation and characterization of a human iPSC cell line expressing
            inducible Cas9 in the ""safe harbor"" AAVS1 locus.
  JOURNAL   Stem Cell Res. 2017 May;21:137-140. doi: 10.1016/j.scr.2017.04.011. 
            Epub 2017 Apr 22.
  PUBMED    28677529
REFERENCE   2  (bases 1 to 1186)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cell 
            Res."; date: "2017-05"; volume: "21:137-140. doi"; pages: " 
            10.1016/j.scr.2017.04.011. Epub 2017 Apr 22"
COMMENT     Sequence Label: pAAVS1-TRE-Cas9- puro-polyA-CAG-rtTA
FEATURES             Location/Qualifiers
     source          1..1186
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    50..887
                     /label=HA-R
                     /note="right homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
ORIGIN
        1 aggataaact ctataaggcg attgatttag cggccgcgaa ttcgccctta gagcagagcc
       61 aggaacccct gtagggaagg ggcaggagag ccaggggcat gagatggtgg acgaggaagg
      121 gggacaggga agcctgagcg cctctcctgg gcttgccaag gactcaaacc cagaagccca
      181 gagcagggcc ttagggaagc gggaccctgc tctgggcgga ggaatatgtc ccagatagca
      241 ctggggactc tttaaggaaa gaaggatgga gaaagagaaa gggagtagag gcggccacga
      301 cctggtgaac acctaggacg caccattctc acaaagggag ttttccacac ggacaccccc
      361 ctcctcacca cagccctgcc aggacggggc tggctactgg ccttatctca caggtaaaac
      421 tgacgcacgg aggaacaata taaattgggg actagaaagg tgaagagcca aagttagaac
      481 tcaggaccaa cttattctga ttttgttttt ccaaactgct tctcctcttg ggaagtgtaa
      541 ggaagctgca gcaccaggat cagtgaaacg caccagacag ccgcgtcaga gcagctcagg
      601 ttctgggaga gggtagcgca gggtggccac tgagaaccgg gcaggtcacg catccccccc
      661 ttccctccca ccccctgcca agctctccct cccaggatcc tctctggctc catcgtaagc
      721 aaaccttaga ggttctggca aggagagaga tggctccagg aaatgggggt gtgtcaccag
      781 ataaggaatc tgcctaacag gaggtggggg ttagacccaa tatcaggaga ctaggaagga
      841 ggaggcctaa agatggggct tttctgtcac caatccttgt ccctagtaaa gcttagtact
      901 ggtcgagttt actcctatca gtggatagag aacgtatgtc gagtttactc ccctatcatg
      961 atagaggacg atgtccaagt ttactcctat catgatagaa aacgtatgtc cagttttact
     1021 cccttatcat tgataggaga acgttatgtt caaggttaac tctctatcag tgatagaaga
     1081 acgtatggtc gagttttatt ccattatcat tgaataggaa acgttatgtc cagggtttac
     1141 ctccccatac aggtaatagg aaacgttgag gtctcagagg gtatgc
//
