LOCUS       sequence-20289-e         819 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 819)
  AUTHORS   Schwarzbauer JE, Tamkun JW, Lemischka IR, Hynes RO
  TITLE     Three different fibronectin mRNAs arise by alternative splicing 
            within the coding region.
  JOURNAL   Cell. 1983 Dec . 35(2 Pt 1):421-31.
  PUBMED    6317187
REFERENCE   2  (bases 1 to 819)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 1983 
            Dec . 35(2 Pt 1):421-31."
COMMENT     Sequence Label: pSR270
FEATURES             Location/Qualifiers
     source          1..819
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(267..285)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(268..285)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(575..594)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 gcctacggat gactcatgct ttgaccctta cacggtttcc cattacgccg ttggagagga
       61 atgggagcgg ttatctgact ctggctttaa gctcacttgc cagtgcttgg gctttggcag
      121 tggtcatttc agatgcgatt catctaaatg gtgccatgac aacggtgtca actacaagat
      181 cggagagaag tgggatcgtc agggagaaaa tggccagcgg atgagctgca catgtctcgg
      241 gaatggaaag ggagaattcc gtgtattcta tagtgtcacc taaatcgtat gtgtatgata
      301 cataaggtta tgtattaatt gtagccgcgt tctaacgaca atatgtacaa gcctaattgt
      361 gtagcatctg gcttactgaa gcagacccta tcatctctct cgtaaactgc cgtcagagtc
      421 ggtttggttg gacgaacctt ctgagtttct ggtaacgccg tcccgcaccc ggaaatggtc
      481 agcgaaccaa tcagcagggt catcgctagc cagatcctct acgccggacg catcgtggcc
      541 ggcatcaccg gcgccacagg tgcggttgct ggcgcctata tcgccgacat caccgatggg
      601 gaagatcggg ctcgccactt cgggctcatg agcgcttgtt tcggcgtggg tatggtggca
      661 ggccccgtgg ccgggggact gttgggcgcc atctccttgc atgcaccatt ccttgcggcg
      721 gcggtgctca acggcctcaa cctactactg ggctgcttcc taatgcagga gtcgcataag
      781 ggagagcgtc gaatggtgca ctctcagtac aatctgctc
//
