LOCUS       sequence-202890-        6952 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Zebrafish Inhibitor of kappa B kinase alpha with mutation at 
            position 179 from cysteine to alanine.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6952)
  AUTHORS   Lisse TS, Rieger S
  TITLE     IKKalpha regulates human keratinocyte migration through surveillance
            of the redox environment.
  JOURNAL   J Cell Sci. 2017 Mar 1;130(5):975-988. doi: 10.1242/jcs.197343. Epub
            2017 Jan 25.
  PUBMED    28122935
REFERENCE   2  (bases 1 to 6952)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1242/jcs.197343"; journalName: "J Cell Sci"; date: "2017-03-1- 
            1"; volume: "130"; issue: "5"; pages: "975-988"
COMMENT     Sequence Label: ptdTomato-N1-CMVL-ikk1FL(C179A)-tdTomato
FEATURES             Location/Qualifiers
     source          1..6952
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      2848..2857
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2854..3555
                     /codon_start=1
                     /product="dimeric variant of DsRed fluorescent protein 
                     (Shaner et al., 2004)"
                     /label=dTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLYGMDELYK"
     primer_bind     complement(2987..3005)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3255..3274
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     3449..3472
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     polyA_signal    3680..3801
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3717..3736)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3771..3790
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3808..4263)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3945..3966)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4157..4176
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4290..4394
                     /gene="bla"
                     /label=AmpR promoter
     promoter        4396..4753
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        4396..4587
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(4419..4439)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4604..4739
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4788..5582
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4788..5582
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4842..4861)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5452..5471
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5770..5789)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5814..5861
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6190..6778
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6679..6698
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagctgtc
      541 aatatggaga aacccccttt cagacagaat caggtctgtg gctcatgggt tatgaaggag
      601 aggcttggaa caggaggatt tggccatgtc tatttgtatc agaatcagga aacatcagaa
      661 aaaatagctg tgaagctctg tcgccttgag cttaattcaa aaaacaaaga cagatggagt
      721 cgagagatcc agattatgaa aaagctaaag caccttaatg tggtcacagc aaaggatgtg
      781 cctgaggaga tgatgcacat tgccttgaat gatttaccac ttttagccat ggagtattgt
      841 tccaaaggag atcttcgtaa gttgttgagc aaacctgaaa attgctgtgg gttgaaagag
      901 agtgaggtgt tgtctctact caatgatgtt ggttctggaa tccagtacct tcatgagaat
      961 aagatcatac acagggatct caagccagag aatattgtgc tgcaagaaat taatggaaag
     1021 cttgttcaca agataatcga ccttggttat gccaaagacc tggaccaggg gagcttagcc
     1081 acttcatttg tggggacttt acaatatctg gctccagagc tttttgaagg caagtcctac
     1141 acagtcactg tggacttttg gagctttggc accatgatct ttgaatgctg ctgtggattt
     1201 cgtccatttc ttcacaatct tcagcctgtg cagtggacaa gcaaagtgcg aaataaaggt
     1261 ccgaaggata taatggctgt ggaagatatg aatggcgagg tccgcttttc cacccacctt
     1321 ccatacccaa acaatcttag caggacacta ctggagccct tggaaggcct tttacagctg
     1381 atgctcaaat gggaccctgt acagagagga ttaggactga acacagattc aaaacagccc
     1441 cagtgctttg ttcttctaga tcaaatactg agcatgaagg ttgtgcacat cttgaacatg
     1501 actacgaccc aggttcactc atttttgcta agcccagatg aagggcttca ctctctgcaa
     1561 cagaggattg agaacgaaac caaaattgaa cttttgaatc aagatcttct gcaagaaaca
     1621 ggagtcatgt tagatcccag gaaacctgct gcacagtgtg tgttagatgg agtaagagga
     1681 tgggacagct acattgtata tctgtttgac aagagtttga ccaagtacat gggaccttta
     1741 actgctagaa cacttcccga aagtgtcaac tttattgttc gggaaaccaa aactcagctc
     1801 ccactgagta ccctaaaaaa ggtttggggt gaggcggtta gctacatttg tggccccaga
     1861 gaagactaca gccgcctctt ccagggccag cgtgcagcca tgctgagtct tcttcgcttc
     1921 aatacaaatt tgacaagata caagaacatg atgttctcct tttcacaaca gctcaaagca
     1981 aagctggact tttttaagac cagcatacag tacgacttgg agaaatacag tgatcagatg
     2041 cagtacggca tatcttcaga aaagatgctg aaggcttggc acgaaaatga agagagggct
     2101 gctgcttttg cacaggtggc agagattggt catctggatg aggaaataat ggcgttgcat
     2161 tcggaaatcg ttgagttaca gagaagtcct tatgctcgtc gtcaaggaga tgtcatggag
     2221 cagttgcaag agaaagccat tgaactctac aaacagttga aggcaaaatg caaaatgcca
     2281 gaccctcagc atggctacag cgacagctct gagatggtga aggtcatagt acagactgtc
     2341 cagaatcaag accgggtgct aagagatcta tatgcacacc tcagtaaaat ccctctgagt
     2401 aagcagaaga tcatcgacct cttccctaaa atcgagagaa cgctggaatg cataaaggag
     2461 gcagacacca cagtcatgca gatgcagatc aagagacaga gagagttctg gcacttactg
     2521 aagattgcct gtgcacaaaa caccacacgg agctcagtga gtcagagcgg agaaatgccc
     2581 tcctcgctgt ctacttggaa ccagactcaa gcccagtgtt cctcccgtct ccccatgtcc
     2641 ttgcaagtgc cacacgaggg tgattcagtt aatcatttac tggaggagaa tcagcgttac
     2701 ttgactcagc tcaccagttt actgcaagaa accaccgagg agaaatcaga gagcataatg
     2761 gctcaggact ggagctggac aaaatacgaa tccctggttg ctaaatcagc gcgtttgtcg
     2821 gtaccgcggg cccgggatcc accggtcgcc accatggtga gcaagggcga ggaggtcatc
     2881 aaagagttca tgcgcttcaa ggtgcgcatg gagggctcca tgaacggcca cgagttcgag
     2941 atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg
     3001 accaagggcg gccccctgcc cttcgcctgg gacatcctgt ccccccagtt catgtacggc
     3061 tccaaggcgt acgtgaagca ccccgccgac atccccgatt acaagaagct gtccttcccc
     3121 gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggtctggt gaccgtgacc
     3181 caggactcct ccctgcagga cggcacgctg atctacaagg tgaagatgcg cggcaccaac
     3241 ttcccccccg acggccccgt aatgcagaag aagaccatgg gctgggaggc ctccaccgag
     3301 cgcctgtacc cccgcgacgg cgtgctgaag ggcgagatcc accaggccct gaagctgaag
     3361 gacggcggcc actacctggt ggagttcaag accatctaca tggccaagaa gcccgtgcaa
     3421 ctgcccggct actactacgt ggacaccaag ctggacatca cctcccacaa cgaggactac
     3481 accatcgtgg aacagtacga gcgctccgag ggccgccacc acctgttcct gtacggcatg
     3541 gacgagctgt acaagtaggc ggccgcgact ctagatcata atcagccata ccacatttgt
     3601 agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat
     3661 gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa
     3721 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     3781 caaactcatc aatgtatctt aaggcgtaaa ttgtaagcgt taatattttg ttaaaattcg
     3841 cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc
     3901 cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga
     3961 gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg
     4021 atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag
     4081 cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga
     4141 acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg
     4201 tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg
     4261 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     4321 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     4381 gaaaaaggaa gagtcctgag gcggaaagaa ccagctgtgg aatgtgtgtc agttagggtg
     4441 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     4501 agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     4561 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc
     4621 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     4681 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     4741 aggcttttgc aaagatcgat caagagacag gatgaggatc gtttcgcatg attgaacaag
     4801 atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg
     4861 cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc
     4921 cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcaa gacgaggcag
     4981 cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca
     5041 ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat
     5101 ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata
     5161 cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac
     5221 gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc
     5281 tcgcgccagc cgaactgttc gccaggctca aggcgagcat gcccgacggc gaggatctcg
     5341 tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg
     5401 gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta
     5461 cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg
     5521 gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct
     5581 gagcgggact ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga
     5641 tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc
     5701 cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc accctagggg
     5761 gaggctaact gaaacacgga aggagacaat accggaagga acccgcgcta tgacggcaat
     5821 aaaaagacag aataaaacgc acggtgttgg gtcgtttgtt cataaacgcg gggttcggtc
     5881 ccagggctgg cactctgtcg ataccccacc gagaccccat tggggccaat acgcccgcgt
     5941 ttcttccttt tccccacccc accccccaag ttcgggtgaa ggcccagggc tcgcagccaa
     6001 cgtcggggcg gcaggccctg ccatagcctc aggttactca tatatacttt agattgattt
     6061 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     6121 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     6181 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     6241 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     6301 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     6361 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     6421 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     6481 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     6541 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     6601 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     6661 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     6721 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     6781 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     6841 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgccatgc attagttatt
     6901 aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc cg
//
