LOCUS       sequence-202896-        8881 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector expressing synonymous tandem MS2 coat protein 
            fused to synonymous tandem Halo-tag for mRNA labeling.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8881)
  AUTHORS   Voigt F, Zhang H, Cui XA, Triebold D, Liu AX, Eglinger J, Lee ES, 
            Chao JA, Palazzo AF
  TITLE     Single-Molecule Quantification of Translation-Dependent Association 
            of mRNAs with the Endoplasmic Reticulum.
  JOURNAL   Cell Rep. 2017 Dec 26;21(13):3740-3753. doi: 
            10.1016/j.celrep.2017.12.008.
  PUBMED    29281824
REFERENCE   2  (bases 1 to 8881)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2017.12"; journalName: "Cell Rep"; date: 
            "2017-12-26- 26"; volume: "21"; issue: "13"; pages: "3740-3753"
COMMENT     Sequence Label: phage UbiC NLS HA stdMCP stdHalo
FEATURES             Location/Qualifiers
     source          1..8881
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..399
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     regulatory      407..416
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             419..439
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             443..469
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             443..469
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             470..493
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     regulatory      1226..1235
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1232..2119
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="MEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSY
                     VWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIH
                     DWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIID
                     QNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVE
                     EYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLI
                     GSEIARWLSTLEISG"
     CDS             2153..3037
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="EIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSYV
                     WRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHD
                     WGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQ
                     NVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVEE
                     YMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLIG
                     SEIARWLSTLEISG"
     misc_feature    3054..3642
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3107..3127)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3525..3536)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3717..3950
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(4069..4087)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4155..4259
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4260..4328,4329..5120)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4478..4497)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5291..5879
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5780..5799
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6033..6050
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6290..6311
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6326..6331,6332..6349,6350..6356)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6364..6380
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6369..6391
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6388..6404
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6388..6404
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6454..6473
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             6665..7298
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    7345..7470
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7967..8200
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8385..8429
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8722..8839
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggcctccgcg ccgggttttg gcgcctcccg cgggcgcccc cctcctcacg gcgagcgctg
       61 ccacgtcaga cgaagggcgc agcgagcgtc ctgatccttc cgcccggacg ctcaggacag
      121 cggcccgctg ctcataagac tcggccttag aaccccagta tcagcagaag gacattttag
      181 gacgggactt gggtgactct agggcactgg ttttctttcc agagagcgga acaggcgagg
      241 aaaagtagtc ccttctcggc gattctgcgg agggatctcc gtggggcggt gaacgccgat
      301 gattatataa ggacgcgccg ggtgtggcac agctagttcc gtcgcagccg ggatttgggt
      361 cgcggttctt gtttgtggat cgctgtgatc gtcacttggc ggccgcgcca ccatgggccc
      421 aaaaaagaaa agaaaagttg gctaccccta cgacgtgccc gactacgccc tggaagttct
      481 gttccagggg cccctgttat taattaacgc ttctaacttt actcagttcg ttctcgtcga
      541 caatggcgga actggcgacg tgactgtcgc cccaagcaac ttcgctaacg ggatcgctga
      601 atggatcagc tctaactcgc gttcacaggc ttacaaagta acctgtagcg ttcgtcagag
      661 ctctgcgcag aatcgcaaat acaccatcaa agtcgaggtg cctaaaggcg cctggcgttc
      721 gtacttaaat atggaactaa ccattccaat tttcgccacg aattccgact gcgagcttat
      781 tgttaaggca atgcaaggtc tcctaaaaga tggaaacccg attccctcag caatcgcagc
      841 aaactccggc atctacgcca tggccagcaa cttcacccag ttcgtgctgg tggacaacgg
      901 cggcaccggc gacgtgaccg tggcccccag caacttcgcc aacggcatcg ccgagtggat
      961 cagcagcaac agcagaagcc aggcctacaa ggtgacctgc agcgtgagac agagcagcgc
     1021 ccagaacaga aagtacacca tcaaggtgga ggtgcccaag ggcgcctgga gaagctacct
     1081 gaacatggag ctgaccatcc ccatcttcgc caccaacagc gactgcgagc tgatcgtgaa
     1141 ggccatgcag ggcctgctga aggacggcaa ccccatcccc agcgccatcg ccgccaacag
     1201 cggcatctac gccgacccac cggtcgccac catggaaatc ggtactggct ttccattcga
     1261 cccccattat gtggaagtcc tgggcgagcg catgcactac gtcgatgttg gtccgcgcga
     1321 tggcacccct gtgctgttcc tgcacggtaa cccgacctcc tcctacgtgt ggcgcaacat
     1381 catcccgcat gttgcaccga cccatcgctg cattgctcca gacctgatcg gtatgggcaa
     1441 atccgacaaa ccagacctgg gttatttctt cgacgaccac gtccgcttca tggatgcctt
     1501 catcgaagcc ctgggtctgg aagaggtcgt cctggtcatt cacgactggg gctccgctct
     1561 gggtttccac tgggccaagc gcaatccaga gcgcgtcaaa ggtattgcat ttatggagtt
     1621 catccgccct atcccgacct gggacgaatg gccagaattt gcccgcgaga ccttccaggc
     1681 cttccgcacc accgacgtcg gccgcaagct gatcatcgat cagaacgttt ttatcgaggg
     1741 tacgctgccg atgggtgtcg tccgcccgct gactgaagtc gagatggacc attaccgcga
     1801 gccgttcctg aatcctgttg accgcgagcc actgtggcgc ttcccaaacg agctgccaat
     1861 cgccggtgag ccagcgaaca tcgtcgcgct ggtcgaagaa tacatggact ggctgcacca
     1921 gtcccctgtc ccgaagctgc tgttctgggg caccccaggc gttctgatcc caccggccga
     1981 agccgctcgc ctggccaaaa gcctgcctaa ctgcaaggct gtggacatcg gcccgggtct
     2041 gaatctgctg caagaagaca acccggacct gatcggcagc gagatcgcgc gctggctgtc
     2101 tactctggag atttccggtg ggagtggcgg ttctggcggt agcggagggg gtgagatcgg
     2161 caccggcttc ccctttgacc ctcactacgt ggaggtgctg ggcgagagga tgcactatgt
     2221 ggacgtggga ccaagagatg gaacccccgt gctgttcctg cacggcaacc ccacaagctc
     2281 ctacgtgtgg cggaatatca tcccacacgt ggcccccaca cacagatgca tcgccccaga
     2341 tctgatcggc atgggcaagt ccgacaagcc cgatctgggc tatttctttg acgatcacgt
     2401 gcggttcatg gacgccttta tcgaggccct gggcctggag gaggtggtgc tggtcatcca
     2461 cgattggggc tctgccctgg gctttcactg ggccaagcgg aaccccgaga gagtgaaggg
     2521 catcgccttc atggagttta tcagaccaat cccaacctgg gatgagtggc ctgagttcgc
     2581 cagagagaca ttccaggcct ttcggaccac agacgtgggc agaaagctga tcatcgatca
     2641 gaacgtgttt atcgaaggca ccctgcctat gggagtggtg aggccactga cagaggtgga
     2701 gatggaccac taccgcgagc ctttcctgaa tccagtggat agggagcctc tgtggcgctt
     2761 tcccaacgag ctgcctatcg ccggcgagcc agccaatatc gtggccctgg tggaggagta
     2821 tatggactgg ctgcaccagt cccctgtgcc aaagctgctg ttctggggca ccccaggcgt
     2881 gctgatccct cccgcagagg cagcaaggct ggccaagtct ctgcccaatt gtaaggccgt
     2941 ggatatcggc cctggcctga acctgctgca ggaggacaat cctgatctga tcggcagcga
     3001 gatcgcccgc tggctgtcta cactggagat cagcggctaa atcgatagat cctaatcaac
     3061 ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta
     3121 cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt
     3181 tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg
     3241 ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg
     3301 gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca
     3361 cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca
     3421 ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctgtg
     3481 ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag
     3541 cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc
     3601 gccctcagac gagtcggatc tccctttggg ccgcctcccc gcctgagatc ctttaagacc
     3661 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga
     3721 agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg ggtctctctg
     3781 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     3841 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     3901 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc
     3961 atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag
     4021 gcccgggtta attaaggaaa gggctagatc attcttgaag acgaaagggc ctcgtgatac
     4081 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt
     4141 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     4201 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     4261 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     4321 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     4381 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     4441 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     4501 gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     4561 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     4621 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     4681 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     4741 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     4801 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     4861 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     4921 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     4981 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     5041 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     5101 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     5161 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     5221 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     5281 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     5341 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     5401 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     5461 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     5521 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     5581 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     5641 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     5701 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     5761 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     5821 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     5881 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     5941 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     6001 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     6061 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagcaagc
     6121 tcatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
     6181 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctccccgt
     6241 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
     6301 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
     6361 gaattgtgag cggataacaa tttcacacag gaaacagcta tgacatgatt acgaatttca
     6421 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     6481 cttatcatgt ctggatcaac tggataactc aagctaacca aaatcatccc aaacttccca
     6541 ccccataccc tattaccact gccaattacc tgtggtttca tttactctaa acctgtgatt
     6601 cctctgaatt attttcattt taaagaaatt gtatttgtta aatatgtact acaaacttag
     6661 tagttggaag ggctaattca ctcccaaaga agacaagata tccttgatct gtggatctac
     6721 cacacacaag gctacttccc tgattagcag aactacacac cagggccagg ggtcagatat
     6781 ccactgacct ttggatggtg ctacaagcta gtaccagttg agccagataa ggtagaagag
     6841 gccaataaag gagagaacac cagcttgtta caccctgtga gcctgcatgg gatggatgac
     6901 ccggagagag aagtgttaga gtggaggttt gacagccgcc tagcatttca tcacgtggcc
     6961 cgagagctgc atccggagta cttcaagaac tgctgatatc gagcttgcta caagggactt
     7021 tccgctgggg actttccagg gaggcgtggc ctgggcggga ctggggagtg gcgagccctc
     7081 agatcctgca tataagcagc tgctttttgc ctgtactggg tctctctggt tagaccagat
     7141 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     7201 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     7261 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg
     7321 aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc
     7381 acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc
     7441 tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga
     7501 tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta
     7561 tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa
     7621 ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt
     7681 agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa
     7741 gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca
     7801 cagcaagcgg ccggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga
     7861 gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca
     7921 aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc
     7981 ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac
     8041 aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg
     8101 aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa
     8161 tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg
     8221 gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg
     8281 aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa
     8341 gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat
     8401 tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt
     8461 ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg
     8521 ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc
     8581 acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa ggtggagaga
     8641 gagacagaga cagatccatt cgattagtga acggatctcg acggtatcgc cgaattcaca
     8701 aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg
     8761 ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat
     8821 tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccag tttggactag
     8881 t
//
