LOCUS       sequence-202898-        9752 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses ST-Renilla reporter in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9752)
  AUTHORS   Voigt F, Zhang H, Cui XA, Triebold D, Liu AX, Eglinger J, Lee ES, 
            Chao JA, Palazzo AF
  TITLE     Single-Molecule Quantification of Translation-Dependent Association 
            of mRNAs with the Endoplasmic Reticulum.
  JOURNAL   Cell Rep. 2017 Dec 26;21(13):3740-3753. doi: 
            10.1016/j.celrep.2017.12.008.
  PUBMED    29281824
REFERENCE   2  (bases 1 to 9752)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2017.12"; journalName: "Cell Rep"; date: 
            "2017-12-26- 26"; volume: "21"; issue: "13"; pages: "3740-3753"
COMMENT     Sequence Label: pSF4 TetCMV intron 4xGCN4 Renilla FKBP STOP 24...
FEATURES             Location/Qualifiers
     source          1..9752
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..19
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    37..55
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    73..91
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    109..127
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    145..163
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    181..199
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    217..235
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     280..304
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          381..513
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     496..515
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        558..576
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      580..589
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             655..711
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             727..783
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             871..927
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             961..1890
                     /codon_start=1
                     /product="Renilla luciferase"
                     /label=hRluc
                     /note="human codon-optimized"
                     /translation="ASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAENA
                     VIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAWF
                     ELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDIA
                     LIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPREI
                     PLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKVK
                     GLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     CDS             join(1891..1893,1894..2214)
                     /codon_start=1
                     /product="destabilization domain that can be stabilized by 
                     Shield1 in the ProteoTuner(TM) system"
                     /label=DD
                     /note="L106P mutant of FKBP12"
                     /translation="MGVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKVDSSRDRNK
                     PFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVEL
                     LKPE"
     CDS             1894..2217
                     /codon_start=1
                     /product="destabilization domain that can be stabilized by 
                     Shield1 in the ProteoTuner(TM) system"
                     /label=DD-C
                     /note="FKBP12 mutant optimized for use as a C-terminal tag"
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKVDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KPE"
     polyA_signal    3747..3881
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     3825..3844
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     protein_bind    complement(3989..4022)
                     /label=FRT3
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision with other FRT3 sites
                     (Bosch et al., 2015)"
     primer_bind     complement(4075..4091)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(4107..4129)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     misc_feature    4540..4897
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     LTR             4962..5431
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(5961..5977)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5961..5977)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5974..5996)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5985..6001
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6009..6015,6016..6033,6034..6039))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6054..6075
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6192..6209)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6363..6951)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6443..6462)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7122..7913,7914..7982))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7745..7764
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7983..8087)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8155..8173
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8211..8233)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8333..8352
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8546..8568
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8560..8577
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8561..8577
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(9659..9706)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
ORIGIN
        1 tccctatcag tgatagagaa cgtatgaaga gtttactccc tatcagtgat agagaacgta
       61 tgcagacttt actccctatc agtgatagag aacgtataag gagtttactc cctatcagtg
      121 atagagaacg tatgaccagt ttactcccta tcagtgatag agaacgtatc tacagtttac
      181 tccctatcag tgatagagaa cgtatatcca gtttactccc tatcagtgat agagaacgta
      241 taagctttag gcgtgtacgg tgggcgccta taaaagcaga gctcgtttag tgaaccgtca
      301 gatcgcctgg agcaattcca caacactttt gtcttatacc aactttccgt accacttcct
      361 accctcgtaa aaccggtcag gtaagtatca aggttacaag acaggtttaa ggagaccaat
      421 agaaactggg cttgtcgaga cagagaagac tcttgcgttt ctgataggca cctattggtc
      481 ttactgacat ccactttgcc tttctctcca caggtgtcca ctcccagttc aattacagct
      541 cttaaggcta gagtacttaa tacgactcac tataggctag ccaccatggg cgccatcata
      601 cacgcggccg caatcatcgg tccgggtgga tctggaggtg gaggttctgg aggagaagaa
      661 cttttgagca agaattatca tcttgagaac gaagtggctc gtcttaagaa aggttctggc
      721 agtggagaag aactgctttc aaagaattac cacctggaaa acgaagtagc tcgattaaag
      781 aaaggttcag ggtcgggtga agaactgctc tcaaaagatt atcatttgga aaatgaggtt
      841 gcacgtttaa aaaagggaag tggcagtggt gaggaacttc tgtcgaaaaa ttatcatctc
      901 gagaatgaag tagcccgact taaaaagggt tctggctcgg gtcagcggcc gcaccggtca
      961 gcttccaagg tgtacgaccc cgagcaacgc aaacgcatga tcactgggcc tcagtggtgg
     1021 gctcgctgca agcaaatgaa cgtgctggac tccttcatca actactatga ttccgagaag
     1081 cacgccgaga acgccgtgat ttttctgcat ggtaacgctg cctccagcta cctgtggagg
     1141 cacgtcgtgc ctcacatcga gcccgtggct agatgcatca tccctgatct gatcggaatg
     1201 ggtaagtccg gcaagagcgg gaatggctca tatcgcctcc tggatcacta caagtacctc
     1261 accgcttggt tcgagctgct gaaccttcca aagaaaatca tctttgtggg ccacgactgg
     1321 ggggcttgtc tggcctttca ctactcctac gagcaccaag acaagatcaa ggccatcgtc
     1381 catgctgaga gtgtcgtgga cgtgatcgag tcctgggacg agtggcctga catcgaggag
     1441 gatatcgccc tgatcaagag cgaagagggc gagaaaatgg tgcttgagaa taacttcttc
     1501 gtcgagacca tgctcccaag caagatcatg cggaaactgg agcctgagga gttcgctgcc
     1561 tacctggagc cattcaagga gaagggcgag gttagacggc ctaccctctc ctggcctcgc
     1621 gagatccctc tcgttaaggg aggcaagccc gacgtcgtcc agattgtccg caactacaac
     1681 gcctaccttc gggccagcga cgatctgcct aagatgttca tcgagtccga ccctgggttc
     1741 ttttccaacg ctattgtcga gggagctaag aagttcccta acaccgagtt cgtgaaggtg
     1801 aagggcctcc acttcagcca ggaggacgct ccagatgaaa tgggtaagta catcaagagc
     1861 ttcgtggagc gcgtgctgaa gaacgagcag atgggagtgc aggtggaaac catctcccca
     1921 ggagacgggc gcaccttccc caagcgcggc cagacctgtg tggtgcacta caccgggatg
     1981 cttgaagatg gaaagaaagt cgattcctcc cgggacagaa acaagccctt taagtttatg
     2041 ctaggcaagc aggaggtgat ccgaggctgg gaagaagggg ttgcccagat gagtgtgggt
     2101 cagagagcca aactgactat atctccagat tatgcctatg gtgccactgg gcacccaggc
     2161 atcatcccac cacatgccac tctcgtcttc gatgtggagc ttctaaaacc ggaataaggg
     2221 ccagggaaga actggctcgg aaagaggacc ccacatgttg tagcttcaag tttaaacgga
     2281 tccaacctac aaacgggtgg aggatcaccc cacccgacac ttcacaatca aggggtacaa
     2341 tacacaaggg tggaggaaca ccccaccctc cagacacatt acacagaaat ccaatcaaac
     2401 agaagcacca tcagggcttc tgctaccaaa tttatctcaa aaaactacaa caaggaatca
     2461 ccatcaggga ttccctgtgc aatatacgtc aaacgagggc cacgacggga ggacgatcac
     2521 gcctcccgaa tatcggcatg tctggctttc gaattcagtg cgtggagcat cagcccacgc
     2581 agccaatcag agtcgaatac aagtcgactt tcgcgaagag catcagcctt cgcgccattc
     2641 ttacacaaac cacactctcc cctacaggaa cagcatcagc gttcctgccc agtacccaac
     2701 tcaagaaaat ttatgtcccc atgcagcatc agcgcatggg ccccaagaat acatccccaa
     2761 caaaatcaca tccgagcacc aacagggctc ggagtgttgt ttcttgtcca actggacaaa
     2821 ccctccatgg accatcaggc catggactct caccaacaag acaaaaacta ctcttctcga
     2881 agcagcatca gcgcttcgaa acactcgagc atacattgtg cctatttctt gggtggacga
     2941 tcacgccacc catgctctca cgaatttcaa aacacggaca aggacgagca ccaccagggc
     3001 tcgtcgttcc acgtccaata cgattactta cctttcggga tcacgatcac ggatcccgca
     3061 gctacatcac ttccactcag gacattcaag catgcacgat cacggcatgc tccacaagtc
     3121 tcaaccacag aaactaccaa atgggttcag caccagcgaa cccactccta cctcaaacct
     3181 cttcccacaa aactggcaag caggatcacc gcttgcccat tccaacatac caaatcaaaa
     3241 acaattactg gtacagcatc agcgtaccag cccacatctc tcactactat caaaaaccaa
     3301 accgttcagc aacagcgaac ggtacacacg gaaaaatcaa ctggtttaca aatacgaaag
     3361 acgatcacgc tttcgtccag cgcaaactat tacgaaaaac atccgacggg aagagcaaca
     3421 gccttcccgc ggcggaaaac ctcacaaaaa cacgacaaac ggatgcacga acacggcatc
     3481 cgccgacaac ccacaaactt acaaccaggc aaacggtgca ggatcaccgc accgtacatc
     3541 aaacacctca gatctcattt cgcgcgggat ccagaccacc tcccctgcga gctaagctgg
     3601 acagccaatg acgggtaaga gagtgacatt tttcactaac ctaagacagg agggccgtca
     3661 gagctactgc ctaatccaaa gacgggtaaa agtgataaaa atgtatcact ccaacctaag
     3721 acaggcgcag cttccgaggg atttgagatc cagacatgat aagatacatt gatgagtttg
     3781 gacaaaccaa aactagaatg cagtgaaaaa aatgccttat ttgtgaaatt tgtgatgcta
     3841 ttgccttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc
     3901 attttatgtt tcaggttcag ggggaggtgt gggaggtttt ttaaagcaag taaaacctct
     3961 acaaatgtgg tatggctgat tatgatctga agttcctata ctatttgaag aataggaact
     4021 tcggaatagg aacttcggat ctcatatggc catatggggg cgcctagaga aggagtgagg
     4081 gctggataaa gggaggatcg aggcggggtc gaacgaggag gttcaagggg gagagacggg
     4141 gcggatggag gaagaggagg cggaggctta gggtgtacaa agggcttgac ccagggaggg
     4201 gggtcaaaag ccaaggcttc ccaggtcacg atgtagggga cctggtctgg gtgtccatgc
     4261 gggccaggtg aaaagacctt gatcttaacc tgggtgatga ggtctcggtt aaaggtgccg
     4321 tctcgcggcc atccgacgtt aaaggttggc cattctgcag agcagaaggt aacccaacgt
     4381 ctcttcttga catctaccga ctggttgtga gcgatccgct cgacatcttt ccagtgacct
     4441 aaggtcaaac ttaagggagt ggtaacaggc tagcccgggc ccatattttc agacaaatac
     4501 agaaacacag tcagacagag acaacacaga acgatgctgc agcagacaag acgcgcggcg
     4561 cggcttcggt cccaaaccga aagcaaaaat tcagacggag gcgggaactg ttttaggttc
     4621 tcgtctccta ccagaaccac atatccctcc tctaaggggg gtgcaccaaa gagtcctaaa
     4681 cgatcgggat tttaggactc aggtcgggcc acaaaaacgg cccccgaagt ccctgggacg
     4741 tctcccaggg ttgcggccgg gtgttccgaa ctcgtcagtt ccaccacggg tccgccagat
     4801 acagagctag ttagctaact agtaccgacg caggcgcata aaatcagtca tagacactag
     4861 acaatcggac agacacagat aagttgctgg ccagcttacc tcccggtggt gggtcggtgg
     4921 tccctgggca ggggtctccc gatcccggac gagcccccaa atgaaagacc cccgctgacg
     4981 ggtagtcaat cactcagagg agaccctccc aaggaacagc gagaccacaa gtcggatgca
     5041 actgcaagag ggtttattgg atacacgggt acccgggcga ctcagtcaat cggaggactg
     5101 gcgcgccgag tgaggggttg tgggctcttt tattgagctc ggggagcaga agcgcgcgaa
     5161 cagaagcgag aagcgaactg attggttagt tcaaataagg cacagggtca tttcaggtcc
     5221 ttggggcacc ctggaaacat ctgatggttc tctagaaact gctgagggct ggaccgcatc
     5281 tggggaccat ctgttcttgg ccctgagccg gggcaggaac tgcttaccac agatatcctg
     5341 tttggcccat attcagctgt tccatctgtt cttggccctg agccggggca ggaactgctt
     5401 accacagata tcctgtttgg cccatattca gctgttccat ctgttcctga ccttgatctg
     5461 aacttttcta ttctcagtta tgtatttttc catgccttgc aaaatggcgt tacttaagct
     5521 agcttgccaa acctacaggt ggggtctttc acatgtatat gtcaaaaata aaaatcaact
     5581 aattgactag taattaatat gactggcata atgggaaatt gatcctgaca gatgcaaact
     5641 ggcttctcag cagcgcattt atgttgtcaa ctgaggaagg aaacgttaat gacagaaact
     5701 ctaagtaatt tccacgttta tctattttta tttatactag ctttggtaac aggaatattg
     5761 cagcattcat gcacattgaa acccttatga aataaaaaca tctgtgcatt taaaatggaa
     5821 ttaacatttt aaatgttaaa aaaagctggc ttagcttccc cccgccccct agggcataga
     5881 acaagtcaaa tgctttatat atttgagttt gggatgtatt aggaaactcc taagagcaaa
     5941 gctagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
     6001 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
     6061 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
     6121 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     6181 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     6241 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     6301 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     6361 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     6421 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     6481 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     6541 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     6601 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     6661 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     6721 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     6781 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     6841 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     6901 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     6961 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttt
     7021 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     7081 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     7141 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     7201 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     7261 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     7321 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     7381 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     7441 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     7501 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     7561 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     7621 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     7681 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     7741 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     7801 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     7861 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     7921 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     7981 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     8041 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     8101 aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg
     8161 cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac
     8221 atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc
     8281 cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta tgcggcatca
     8341 gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg
     8401 agaaaatacc gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga
     8461 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
     8521 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgaa
     8581 ttaattccaa gctaagtgaa agactctgtc tcaaaaacca acctgacacc tgagccggag
     8641 cgttgcctct cactaccata aatatatata cacaacacac acacacacac acacacacac
     8701 acacacacac acacacacaa tcatacacat aaacacaatc acacacacac acatgcacaa
     8761 tcacacacac acaatcacac acaaacatac atacacacaa tcacacacaa ccatacacac
     8821 atatacacaa tcacacacac aaacacaatc acacacacac acatcaatca atcaatcaat
     8881 caatatacca aaaatgatag ctcatacctg taatcttaga tgtgggaagg ctaaaacagc
     8941 aggctaccca tgacttcaag gtcaccctgg gttacagagt agctaatagt ctagcttgaa
     9001 ctatagtgta ggatctttaa aaaaaaatta accaaccaac caacaaacaa acaaacaaac
     9061 aacaacaaca acaaatggaa gacagaggac aaaaaaaaat aaaagaaaaa gtagaaagcc
     9121 aatggagata ctatcttaaa attagaaata tttatgacac attctgaata ctccatgttt
     9181 atttcaagga agccaatgta actttaagta ctatggccat ttaatgtaaa tacttaagaa
     9241 aaaaaaccaa attaattttg atacatgctg catgtgaaag acccccgctg acgggtagtc
     9301 aatcactcag aggagaccct ctcaaggaac agcgagacca caagtcggat gcaactgcaa
     9361 gagggtttat tggatacacg ggtacccggg cgactcagtc aatcggagga ctggcgcccc
     9421 gagtgagggg ttgtgggctc gagctcgggg agcagaagcg cgcgaacaga agtcgagcta
     9481 gcttgccaaa cctacaggtg gggtctttca ttcccccctt tttctggaga ctaaataaaa
     9541 tcttttattt tatctatggc tcgtactcta taggcttcag ctggtgatat tgttgagtca
     9601 aaactagagc ctggaccact gatatcctgt ctttaacaaa ttggactaat ccggatctga
     9661 agttcctata ctttctagag aataggaact tcggaatagg aacttcgcgg ccagatcttt
     9721 tgcggccgct ttgggccggc cgaattcttt ac
//
