LOCUS       sequence-20290-e         745 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 745)
  AUTHORS   Paul JI, Schwarzbauer JE, Tamkun JW, Hynes RO
  TITLE     Cell-type-specific fibronectin subunits generated by alternative 
            splicing.
  JOURNAL   J Biol Chem. 1986 Sep 15. 261(26):12258-65.
  PUBMED    3528152
REFERENCE   2  (bases 1 to 745)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 1986 Sep 15. 261(26):12258-65."
COMMENT     Sequence Label: pEIIIA
FEATURES             Location/Qualifiers
     source          1..745
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(248..266)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(249..266)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(556..575)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 ggcgagctcg aattccgcga ttccatcaaa attgcctggg aaagcccaca ggggcaagtt
       61 tccaggtaca gggtgaccta ctcaagccct gaggatggaa tccatgagct tttccctgcg
      121 cctgatggtg acgaggacac ggcagagctg cacggcctca ggccgggttc tgagtacaca
      181 gtcagtgtgg ttgccttgca cggtggcatg gagagccagc ccctgattgg agtggaattc
      241 cgtgtattct atagtgtcac ctaaatcgta tgtgtatgat acataaggtt atgtattaat
      301 tgtagccgcg ttctaacgac aatatgtaca agcctaattg tgtagcatct ggcttactga
      361 agcagaccct atcatctctc tcgtaaactg ccgtcagagt cggtttggtt ggacgaacct
      421 tctgagtttc tggtaacgcc gtcccgcacc cggaaatggt cagcgaacca atcagcaggg
      481 tcatcgctag ccagatcctc tacgccggac gcatcgtggc cggcatcacc ggcgccacag
      541 gtgcggttgc tggcgcctat atcgccgaca tcaccgatgg ggaagatcgg gctcgccact
      601 tcgggctcat gagcgcttgt ttcggcgtgn gtatggtggc aggccccgtg gccgggggac
      661 tgttgggcgc catctccttg catgcaccat tccttgcggc ggcggtgctc aacggcctca
      721 acctactact gggctgcttc ctaat
//
